Separation and purification device for protein

Abstract

The utility model relates to a separation and purification device, in particular to a separation and purification device for protein, which comprises a first storage barrel, a placing frame, a second storage barrel, a mixing barrel, a motor, a stirring rod and the like, the top of the first storage barrel is movably connected with the placing frame, the second storage barrel is arranged at the top of the placing frame, and the mixing barrel is mounted at the top of the second storage barrel. The first storage cylinder, the placing frame, the second storage cylinder and the mixing cylinder are arranged in a stacked manner and are communicated with one another, a motor is mounted at the top of the mixing cylinder, and an output shaft of the motor extends into the mixing cylinder and is connected with a stirring rod. Through the driving of the motor, the stirring rod can effectively mix a protein solution, a buffer solution and an organic solvent, the reaction rate of the protein solution and the reagent is accelerated, and the efficient mixing promotes the separation and precipitation of protein from the solution.

Description

Technical Field

[0001] The utility model relates to a separation and purification device, in particular to a separation and purification device for protein. Background Art

[0002] Protein separation and purification has a wide range of applications in biochemistry, molecular biology, medicine, and biotechnology. Protein purification exploits the inherent similarities and differences between different proteins. This similarity is used to remove non-protein contamination, while the differences between proteins are exploited to purify the target protein from other proteins. Because each protein varies in size, shape, charge, hydrophobicity, solubility, and biological activity, these differences can be exploited to extract proteins from a mixture.

[0003] In existing separation and purification operations, most of them require the preparation of a protein mixed solution in advance. After the protein is precipitated and extracted from the mixed solution, the mixed solution is chromatographed through a separation and purification device to extract the required protein, thereby completing the separation and purification work of the protein by the separation and purification device. However, since the separation and purification device cannot complete the two processes of preparation and separation and purification at the same time, there is a window period in the work, which affects work efficiency.

[0004] Therefore, in order to solve the above problems, a protein separation and purification device with the function of preparing a protein mixed solution needs to be designed. Utility Model Content

[0005] In order to overcome the shortcoming of the prior art that the two processes of preparation and separation and purification cannot be achieved simultaneously, the technical problem is: to provide a protein separation and purification device with the function of preparing a protein mixed solution.

[0006] A protein separation and purification device includes support feet, a first storage cylinder, a placement frame, a second storage cylinder, a mixing cylinder, a motor and a stirring rod. The top of the first storage cylinder is movably connected to the placement frame, the second storage cylinder is arranged on the top of the placement frame, the side walls of the first storage cylinder and the second storage cylinder are fixedly connected to support feet for fixing, the top of the second storage cylinder is installed, the first storage cylinder, the placement frame, the second storage cylinder and the mixing cylinder are stacked and interconnected, the top of the mixing cylinder is installed with a motor, the output shaft of the motor extends into the interior of the mixing cylinder and is connected to the stirring rod.

[0007] Optionally, the side walls of the first storage tube and the second storage tube are fixedly connected to a mounting plate, the mounting plate is fixedly connected to a third guide rod, the third guide rod is slidably connected to a wedge block, a second spring is wound around the third guide rod, the two ends of the second spring are respectively connected to the mounting plate and the wedge block, and the wedge block is plugged into and fitted with the protruding block of the placement frame.

[0008] Optionally, a first guide rod is fixedly connected to the top of the second storage tube, the first guide rod is slidably connected to a connecting plate, a first spring is wound around the first guide rod, two ends of the first spring are respectively connected to the connecting plate and the end of the first guide rod, the connecting plate is fixedly connected to a sealing plate, the second guide rod is fixedly connected to the sliding groove opened on the side wall of the second storage tube, and the end of the connecting plate away from the sealing plate is slidably connected to the second guide rod.

[0009] Optionally, the top of the second storage tube is rotatably connected to a limiting plate, the limiting plate is L-shaped, and the long end of the limiting plate extends into the interior of the second storage tube to limit the sealing plate.

[0010] Optionally, a cover plate is rotatably connected to the top of the mixing drum, and the cover plate is used to seal the opening of the mixing drum.

[0011] Optionally, a water pump is installed on the top of the mixing drum, and the water inlet and outlet of the water pump are connected to hoses. The end of the hose connected to the water inlet of the water pump is fixedly connected to an extension pipe, and an electric slide rail is installed on the top of the mixing drum, and the slider of the electric slide rail is fixedly connected to the extension pipe.

[0012] Optionally, a valve is provided below the side wall of the first storage cylinder, and the valve is connected to a two-way liquid discharge channel.

[0013] The beneficial effects are: 1. The utility model is driven by a motor, and the stirring rod can effectively mix the protein solution, buffer solution and organic solvent, thereby accelerating the reaction rate of the protein solution and the reagent. This efficient mixing promotes the separation and precipitation of the protein from the solution. At the same time, the chromatographic medium can further separate the protein in the mixed solution according to its own characteristics, thereby achieving efficient protein separation and purification.

[0014] 2. By sliding the wedge block and compressing the second spring, the limit on the placement frame can be easily released, allowing the placement frame to slide outward, thereby easily replacing the chromatography medium. This design not only simplifies the steps of replacing the chromatography medium, but also improves the maintainability and service life of the device.

[0015] 3. The combination of electric slide rails and water pumps can quickly extract the upper layer of waste liquid, reducing the tedious manual operation and improving work efficiency. BRIEF DESCRIPTION OF THE DRAWINGS

[0016] Figure 1 It is a schematic diagram of the three-dimensional structure of the utility model.

[0017] Figure 2 This is a three-dimensional structural sectional view of the mixing cylinder and cover plate of the utility model.

[0018] Figure 3 This is a cross-sectional view of the mixing cylinder and cover plate of the utility model.

[0019] Figure 4This is a sectional view of the three-dimensional structure of the second storage tube of the present invention.

[0020] Figure 5 This is a sectional view of the three-dimensional structure of the placement frame, mounting plate and wedge block of the utility model.

[0021] In the accompanying drawings: 1-support foot, 2-first storage cylinder, 3-placement frame, 4-second storage cylinder, 401-sealing plate, 402-limiting plate, 403-connecting plate, 404-first guide rod, 405-first spring, 406-second guide rod, 5-mixing cylinder, 6-cover plate, 7-motor, 701-stirring rod, 8-water pump, 801-hose, 802-extension tube, 803-electric slide rail, 9-mounting plate, 901-wedge block, 902-third guide rod, 903-second spring, 10-valve. DETAILED DESCRIPTION

[0022] The present invention will be described in detail below with reference to the accompanying drawings and specific embodiments.

[0023] Example: A protein separation and purification device, such as Figure 1 and Figure 2 As shown, it includes a first storage cylinder 2, a placement frame 3, a second storage cylinder 4, a mixing cylinder 5, a motor 7 and a stirring rod 701. The top of the first storage cylinder 2 is movably connected to the placement frame 3, and the second storage cylinder 4 is arranged on the top of the placement frame 3. The side walls of the first storage cylinder 2 and the second storage cylinder 4 are fixedly connected with a supporting foot 1 for support and fixation. The top of the second storage cylinder 4 is installed with a mixing cylinder 5. The first storage cylinder 2, the placement frame 3, the second storage cylinder 4 and the mixing cylinder 5 are stacked and interconnected with each other. The top of the mixing cylinder 5 is installed with a motor 7, and the output shaft of the motor 7 extends into the interior of the mixing cylinder 5 and is connected to the stirring rod 701. Here, the top of the mixing cylinder 5 is rotatably connected to a cover plate 6, which is used to seal the opening of the mixing cylinder 5 so that the output shaft of the motor 7 drives the stirring rod 701 to rotate for mixing and stirring to prevent the liquid from falling into the second storage cylinder 2. A chromatographic medium for separating and purifying proteins is placed in the placement frame 3. At the same time, according to the different properties of different proteins, chromatographic media with different functions can be replaced to obtain corresponding proteins.

[0024] like Figure 1 and Figure 5As shown, the side walls of the first storage tube 2 and the second storage tube 2 are fixedly connected to the mounting plate 9, the mounting plate 9 is fixedly connected to the third guide rod 902, the third guide rod 902 is slidably connected to the wedge block 901, and the third guide rod 902 is wound with a second spring 903. The two ends of the second spring 903 are respectively connected to the mounting plate 9 and the wedge block 901, and the wedge block 901 is plugged into the protruding block of the placement frame 3. Here, when the wedge block 901 is slid upward, the second spring 903 is compressed, so that the wedge block 901 no longer cooperates with the protruding block of the placement frame 3, thereby releasing the limit on the placement frame 3, allowing the placement frame 3 to slide outward, so that the chromatography medium in the placement frame 3 can be replaced.

[0025] like Figure 4 As shown, the top of the second storage cylinder 4 is fixedly connected to a first guide rod 404, the first guide rod 404 is slidably connected to a connecting plate 403, a first spring 405 is wound around the first guide rod 404, the two ends of the first spring 405 are respectively connected to the connecting plate 403 and the end of the first guide rod 404, the connecting plate 403 is fixedly connected to a sealing plate 401, a second guide rod 406 is fixedly connected to the sliding groove opened on the side wall of the second storage cylinder 4, the end of the connecting plate 403 away from the sealing plate 401 is slidably connected to the second guide rod 406, the top of the second storage cylinder 4 is rotatably connected to the limiting plate 402, the limiting plate 402 is L-shaped, and the long end of the limiting plate 402 extends into the interior of the second storage cylinder 4 to limit the sealing plate 401. Here, the limiting plate 402 is rotated so that the limiting plate 402 no longer limits the sealing plate 401. At this time, the connecting plate 403 is slid downward, the first spring 405 is compressed, and the connecting plate 403 drives the sealing plate 401 to move downward. The sealing plate 401 no longer blocks the top opening of the second storage cylinder 4, so that the mixing cylinder 5 is connected to the second storage cylinder 4. The end of the connecting plate 403 away from the sealing plate 401 is fixedly connected to a block that blocks the side wall groove of the second storage cylinder 4 to prevent the mixed solution from splashing.

[0026] like Figure 1 and Figure 3 As shown, a water pump 8 is installed on the top of the mixing drum 5, and the water suction port and the discharge port of the water pump 8 are both connected to a hose 801. The end of the hose 801 connected to the water suction port of the water pump 8 is fixedly connected to an extension tube 802. An electric slide rail 803 is installed on the top of the mixing drum 5, and the slider of the electric slide rail 803 is fixedly connected to the extension tube 802. Here, the electric slide rail 803 can drive the slider of the electric slide rail 803 to slide up and down, so that the slider of the electric slide rail 803 drives the extension tube 802 to move up and down, so that the liquid extraction position of the water pump 8 can be flexibly adjusted to perform precise extraction.

[0027] like Figure 1As shown, a valve 10 is provided below the side wall of the first storage cylinder 2. Here, the valve 10 is connected to a two-way drainage channel. When the switch is rotated clockwise, the left side is drained, and when the switch is rotated counterclockwise, the right side is drained. The valve 10 with a two-way drainage channel can select a drainage outlet to discharge the waste liquid generated by the cleaning device before the secondary separation of the protein, thereby ensuring the purity of the protein.

[0028] When it is necessary to separate and purify the desired protein, this device can be used. First, the cover plate 6 is flipped to open the mixing drum 5 and pour the protein solution, buffer and organic solvent into the mixing drum 5. Then, the motor 7 is started. The output shaft of the motor 7 drives the stirring rod 701 to rotate, thereby mixing the protein solution, buffer and organic solvent, accelerating the reaction rate, and promoting the separation and precipitation of the protein from the protein solution. After completion, the motor 7 is turned off and the electric slide 803 is started. The slider of the electric slide 803 drives the extension tube 802 to move downward, so that the extension tube 802 extends into the mixed solution. After that, the water pump 8 cooperates with the hose 801 and the extension tube 802 to extract the waste liquid generated after the reaction of the mixed solution. Then, the limit plate 402 is rotated so that the limit plate 402 no longer limits the sealing plate 401. At this time, the connecting plate 403 is slid downward, the first spring 405 is compressed, and the connecting plate 403 drives the sealing plate 401 to move downward. The sealing plate 401 no longer blocks the top opening of the second storage tube 4, so that the mixed solution in the mixing drum 5 enters the interior of the second storage tube 4. After the mixed solution enters the second storage cylinder 4, the mixed solution flows downward under the influence of gravity. When the mixed solution flows downward through the placement frame 3, it will flow through the chromatography medium in the placement frame 3. The chromatography medium further separates the protein in the mixed solution according to its own characteristics, so that the required protein falls into the first storage cylinder 2, and the protein is taken out through the valve 10, completing the separation and purification of the protein.

[0029] Finally, it should be noted that the above is only a preferred embodiment of the present invention and is not intended to limit the present invention. Although the present invention has been described in detail with reference to the aforementioned embodiments, those skilled in the art can still modify the technical solutions described in the aforementioned embodiments or make equivalent replacements for some of the technical features therein. Any modifications, equivalent replacements, improvements, etc. made within the spirit and principles of the present invention should be included in the scope of protection of the present invention.

Claims

1. A protein separation and purification device comprising a support leg (1), characterized in that: The invention also comprises a first storage barrel (2), a placement frame (3), a second storage barrel (4), a mixing barrel (5), a motor (7) and a stirring rod (701); the top of the first storage barrel (2) is movably connected to the placement frame (3); the top of the placement frame (3) is provided with the second storage barrel (4); the side walls of the first storage barrel (2) and the second storage barrel (4) are fixedly connected with supporting feet (1) for supporting and fixing; the top of the second storage barrel (4) is provided with a mixing barrel (5); the first storage barrel (2), the placement frame (3), the second storage barrel (4) and the mixing barrel (5) are arranged in a stacked manner and are interconnected; the top of the mixing barrel (5) is provided with a motor (7); the output shaft of the motor (7) extends into the interior of the mixing barrel (5) and is connected to the stirring rod (701).

2. The protein separation and purification device according to claim 1, wherein: The side walls of the first storage tube (2) and the second storage tube (4) are both fixedly connected to a mounting plate (9), the mounting plate (9) is fixedly connected to a third guide rod (902), the third guide rod (902) is slidably connected to a wedge block (901), a second spring (903) is wound around the third guide rod (902), the two ends of the second spring (903) are respectively connected to the mounting plate (9) and the wedge block (901), and the wedge block (901) is plugged into and matched with a protruding block of the placement frame (3).

3. The protein separation and purification device according to claim 2, wherein: A first guide rod (404) is fixedly connected to the top of the second storage cylinder (4), and the first guide rod (404) is slidably connected to the connecting plate (403). A first spring (405) is wound around the first guide rod (404), and the two ends of the first spring (405) are respectively connected to the connecting plate (403) and the end of the first guide rod (404). The connecting plate (403) is fixedly connected to the sealing plate (401). A second guide rod (406) is fixedly connected to the sliding groove provided on the side wall of the second storage cylinder (4), and one end of the connecting plate (403) away from the sealing plate (401) is slidably connected to the second guide rod (406).

4. The protein separation and purification device according to claim 3, wherein: The top of the second storage tube (4) is rotatably connected to a limiting plate (402), the limiting plate (402) is L-shaped, and the long end of the limiting plate (402) extends into the interior of the second storage tube (4) to limit the sealing plate (401).

5. The protein separation and purification device according to claim 4, characterized in that: The top of the mixing cylinder (5) is rotatably connected to a cover plate (6), and the cover plate (6) is used to seal the opening of the mixing cylinder (5).

6. The protein separation and purification device according to claim 5, characterized in that: A water pump (8) is installed on the top of the mixing drum (5), and the water inlet and outlet of the water pump (8) are both connected to a hose (801). The end of the hose (801) connected to the water inlet of the water pump (8) is fixedly connected to an extension tube (802). An electric slide rail (803) is installed on the top of the mixing drum (5), and a slider of the electric slide rail (803) is fixedly connected to the extension tube (802).

7. The protein separation and purification device according to claim 6, characterized in that: A valve (10) is provided below the side wall of the first storage cylinder (2), and the valve (10) is connected to a bidirectional liquid discharge channel.

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