Toxicity test device for zebra fish

Abstract

The utility model discloses a toxicity test device for zebra fish. The toxicity test device comprises a workbench; the at least one toxicity test assembly is detachably arranged on the workbench through a supporting frame, and any one toxicity test assembly comprises a temporary culture cylinder; two rows of liquid medicine treatment cylinders; two rows of fish sieves; the supporting frame comprises supporting legs; a horizontal support plate; a pair of slide rails I; a pair of support frames I; two groups of slide rails II; a pair of support frames II; a plurality of medicine adding bottles; a water supply pipe I; and a waste liquid recovery assembly. According to the toxicity test device for the zebrafish, a plurality of zebrafish of the same specification in the temporary rearing tank can be synchronously moved into two rows or one row of liquid medicine treatment tanks at a time, convenience and rapidness are achieved, the time of placing the zebrafish in the liquid medicine can be kept synchronous, and test preciseness is guaranteed.

Description

Technical Field

[0001] The utility model relates to the technical field of biological toxicity test devices, in particular to a toxicity test device for zebrafish. Background Art

[0002] Zebrafish, as an important model organism, are widely used in toxicity testing in fields such as environmental toxicology and drug development. Existing zebrafish toxicity testing equipment has some shortcomings, such as the tendency for uneven drug concentrations to occur during dosing and the inconvenience of manually placing zebrafish in tanks containing different drug concentrations. Chinese patent application number 202420047216.2 discloses a zebrafish chronic toxicology test device, comprising: a test component (1), wherein the test component (1) comprises a fish tank (101) capable of accommodating zebrafish; a dosing component (2); a reflux component (3); the dosing pipeline comprises a liquid extraction pipe (203) and a liquid inlet pipe (204); one end of the liquid inlet pipe (204) connected to the fish tank (101) is connected to a conducting pipe (206), and the conducting pipe (206) is connected to a plurality of shunt pipes (207), one end of the shunt pipe (207) is connected to the conducting pipe (206), and the other end of the shunt pipe (207) is arranged opposite to the opening of the fish tank (101), and the shunt pipes (207) correspond one to one with the fish tank (101). Among them, the method is connected to the fish tank (101) through a dosing pipeline. After adding the medicine into the fish tank (101), multiple zebrafish need to be added into the fish tank (101) manually in sequence. There is a certain time difference between the time of adding multiple zebrafish into each fish tank (101), which is time-consuming and labor-intensive, and the operation is not convenient. Utility Model Content

[0003] An object of the present invention is to solve at least the above problems and to provide at least the advantages to be described below.

[0004] Another object of the present invention is to provide a toxicity testing device for zebrafish, which can simultaneously move multiple zebrafish of the same size in a temporary holding tank to two rows or one row of drug solution treatment tanks at one time, which is convenient and fast. The time for multiple zebrafish to be placed in the drug solution can also be kept synchronized, ensuring the rigor of the test.

[0005] In order to achieve these purposes and other advantages according to the present invention, a toxicity test device for zebrafish is provided, comprising:

[0006] Workbench;

[0007] At least one group of toxicity test components is detachably arranged on a workbench through a support frame, and any group of toxicity test components includes a temporary holding tank, which is a long strip-shaped breeding tank, and the temporary holding tank is arranged in the middle of the workbench; two rows of liquid medicine treatment tanks are sequentially arranged on both sides of the temporary holding tank, and the arrangement length of the two rows of liquid medicine treatment tanks is less than the length of the temporary holding tank, and the arrangement width of the two rows of liquid medicine treatment tanks is less than the width of the temporary holding tank; two rows of fish sieves are detachably arranged above the temporary holding tank and the two rows of liquid medicine treatment tanks through a support frame, and the two rows of fish sieves are detachably placed in the temporary holding tank, or the two rows of fish sieves are detachably placed in the two rows of liquid medicine treatment tanks in a one-to-one correspondence, and the specifications of any fish sieve are adapted to the specifications of any liquid medicine treatment tank, and the hole spacing of any fish sieve is less than the thickness of the zebrafish body; the support frame includes Support legs, which are arranged on a workbench around two rows of liquid medicine treatment tanks; a horizontal support plate, which is horizontally arranged on the support legs; a pair of slide rails I, which are arranged on the lower surface of the horizontal support plate, and the pair of slide rails I are perpendicular to the length direction of the temporary holding tank; a pair of support frames I, which are slidably arranged on the pair of slide rails I; two sets of slide rails II, which are respectively arranged on the pair of support frames I, and in the vertical direction, the length of the two sets of slide rails II is greater than the height of any liquid medicine treatment tank; a pair of support frames II, which are respectively slidably arranged on the two sets of slide rails II, and two rows of fish screens are respectively detachably arranged on the pair of support frames II; a plurality of dosing bottles, which are respectively connected to at least two rows of liquid medicine treatment tanks through conduits I; a water supply pipe I, the water outlet of which extends to the temporary holding tank and the two rows of liquid medicine treatment tanks; and

[0008] The waste liquid recovery component is arranged below the workbench and is connected to the bottoms of at least two rows of liquid treatment tanks through a waste liquid reflux pipe.

[0009] Preferably, the device further comprises: a plurality of air-permeable covers, which are detachably buckled on the upper openings of the two rows of fish sieves in a one-to-one correspondence.

[0010] Preferably, it further comprises: a water supply pipe II, the water inlet end of which is arranged in the temporary holding tank, and the water outlet end of the water supply pipe II extends to two rows of liquid treatment tanks respectively.

[0011] Preferably, it also includes: a filter box I, which is connected to the temporary holding tank through a circulation pipeline I; a pump I, which is arranged on the circulation pipeline I; two filter boxes II, which are respectively connected to two rows of liquid treatment tanks through two sets of circulation pipelines II; and two pumps II, which are respectively arranged on the two sets of circulation pipelines II.

[0012] Preferably, it further comprises: a bidirectional telescopic rod, both ends of which are hingedly mounted on a pair of support frames II;

[0013] The electric telescopic rod has a base arranged on a workbench, and the front end of the inner rod of the electric telescopic rod is hingedly arranged at the middle part of the two-way telescopic rod.

[0014] Preferably, any row of drug processing tanks includes at least three drug processing tanks;

[0015] The temporary holding tank, two rows of liquid treatment tanks and two rows of fish screens are all made of transparent acrylic plastic.

[0016] The utility model has at least the following beneficial effects:

[0017] The temporary holding tank and the two rows of liquid medicine treatment tanks can be made of transparent corrosion-resistant materials. The temporary holding tank is used to temporarily hold multiple zebrafish of similar specifications for subsequent toxicity tests. The two rows of liquid medicine treatment tanks are respectively set as independent test chambers to meet the test requirements of different toxicity concentrations; the two rows of fish sieves are used to screen zebrafish of the same specifications and conveniently divide them and temporarily hold them in the temporary holding tank. When a toxicity test is required, the two rows or one row of fish sieves and the multiple zebrafish inside can be lifted from the temporary holding tank by the support frame and then placed in two rows or one row of fish sieves at the same time. The drug solution treatment tank is convenient and fast, and the time for multiple zebrafish to be placed in the drug solution can be kept synchronous, ensuring the rigor of the experiment and ensuring that the zebrafish are not damaged; multiple drug adding bottles are used to provide different amounts of drug solution with the same concentration to the two rows of drug solution treatment tanks, or to provide the same amount of drug solution with increasing concentration gradient, which is convenient and fast; when the experiment is completed, or when the experiment is replaced midway, the toxic aquaculture wastewater in the two rows of drug solution treatment tanks can be conveniently discharged into the waste liquid recovery component for waste liquid recovery and filtration treatment, thereby preventing wastewater from polluting the environment.

[0018] In summary, the toxicity test device for zebrafish provided by the utility model can simultaneously move multiple zebrafish of the same size in a temporary holding tank to two rows or one row of drug solution treatment tanks at one time, which is convenient and fast. The time for multiple zebrafish to be placed in the drug solution can also be kept synchronized, ensuring the rigor of the test.

[0019] Other advantages, objectives and features of the present invention will be reflected in part through the following description, and in part will be understood by those skilled in the art through research and practice of the present invention. BRIEF DESCRIPTION OF THE DRAWINGS

[0020] Figure 1 This is a schematic top view of the structure of a toxicity test device for zebrafish according to one embodiment of the present invention;

[0021] Figure 2 This is a front view schematic diagram of a toxicity test device for zebrafish according to another embodiment of the present invention;

[0022] Figure 3 This is a side structural schematic diagram of a toxicity test device for zebrafish according to one embodiment of the present invention;

[0023] Figure 4This is a front view schematic diagram of a toxicity test device for zebrafish according to another embodiment of the present invention;

[0024] Figure 5 This is a front view schematic diagram of the structure of a toxicity test device for zebrafish according to another embodiment of the present invention, wherein a bidirectional telescopic rod and an electric telescopic rod are shown;

[0025] Figure 6 This is a top view of the structure of a pair of support frames II in another embodiment of the present invention, wherein a bidirectional telescopic rod and an electric telescopic rod are shown. DETAILED DESCRIPTION

[0026] The present invention will be described in further detail below in conjunction with the accompanying drawings so that those skilled in the art can implement the invention with reference to the description.

[0027] It should be understood that terms such as “having”, “including” and “comprising” used herein do not preclude the existence or addition of one or more other elements or combinations thereof.

[0028] like Figure 1 、 2 As shown in FIG. 3 , the utility model provides a toxicity test device for zebrafish, comprising:

[0029] Workbench 10;

[0030] At least one set of toxicity test components 20 is detachably arranged on the workbench through a support frame. Any set of toxicity test components includes a temporary holding tank 201, which is a long strip-shaped aquaculture tank, and the temporary holding tank is arranged in the middle of the workbench; two rows of liquid medicine treatment tanks 202 are arranged in sequence on both sides of the temporary holding tank, and the arrangement length of the two rows of liquid medicine treatment tanks is less than the length of the temporary holding tank, and the arrangement width of the two rows of liquid medicine treatment tanks is less than the width of the temporary holding tank; in actual application, the temporary holding tank and the two rows of liquid medicine treatment tanks can also be equipped with temperature sensors and heating pipes to adjust the temperature in the tank. The water temperature is adjusted, and a lamp is set to adjust the light intensity; two rows of fish screens 203 are detachably arranged above the temporary holding tank and the two rows of liquid medicine treatment tanks through a support frame, and the two rows of fish screens are detachably placed in the temporary holding tank, or the two rows of fish screens are detachably placed in the two rows of liquid medicine treatment tanks in a one-to-one correspondence, and the specifications of any fish screen are adapted to the specifications of any liquid medicine treatment tank, and the hole spacing of any fish screen is less than the thickness of the zebrafish body; the support frame 204 includes support legs 2041, which are arranged on the workbench around the two rows of liquid medicine treatment tanks; the horizontal support plate 2042 , which is horizontally arranged on the support legs; a pair of slide rails I 2043, which are arranged on the lower surface of the horizontal support plate, and the pair of slide rails I are perpendicular to the length direction of the temporary holding tank; a pair of support frames I 2044, which are slidably arranged on the pair of slide rails I; two sets of slide rails II 2045, which are respectively arranged on the pair of support frames I, and in the vertical direction, the length of the two sets of slide rails II is greater than the height of any liquid treatment tank; a pair of support frames II 2046, which are respectively arranged on the two sets of slide rails II for reciprocating sliding up and down, and the two rows of fish screens are respectively detachably arranged On a pair of support frames II; the support frame has a simple structure and can realize the synchronous transfer of multiple zebrafish through manual operation, which is low cost and high efficiency; multiple dosing bottles 205, which are respectively connected to at least two rows of liquid treatment tanks through conduit I; water supply pipe I, whose water outlet extends to the temporary holding tank and the two rows of liquid treatment tanks; water supply pipe I is used to supply water to the temporary holding tank and the two rows of liquid treatment tanks to meet the breeding needs; and a waste liquid recovery component 30, which is set below the workbench and is connected to the bottom of at least two rows of liquid treatment tanks through a waste liquid return pipe. The waste liquid recovery component can be configured to treat the wastewater by sedimentation and activated carbon filtration, etc., and can further reduce heavy metal ions to low-valent states or metal elements by adding reducing agents such as sodium sulfide and iron powder, so as to facilitate subsequent precipitation or separation and removal.

[0031] In this scheme, the temporary holding tank and the two rows of liquid medicine treatment tanks can be made of transparent corrosion-resistant materials. The temporary holding tank is used to temporarily hold multiple zebrafish of similar sizes for subsequent toxicity tests. The two rows of liquid medicine treatment tanks are respectively set as independent test chambers to meet the test requirements of different toxicity concentrations; the two rows of fish sieves are used to screen zebrafish of the same size and conveniently divide them and temporarily hold them in the temporary holding tank. When a toxicity test is required, the two rows or one row of fish sieves and the multiple zebrafish therein can be lifted from the temporary holding tank by the support frame and then placed in two rows or one row of fish sieves simultaneously. The drug solution treatment tank is convenient and fast, and the time for multiple zebrafish to be placed in the drug solution can be kept synchronous, ensuring the rigor of the experiment and ensuring that the zebrafish are not damaged; multiple drug adding bottles are used to provide different amounts of drug solution with the same concentration to the two rows of drug solution treatment tanks, or to provide the same amount of drug solution with increasing concentration gradient, which is convenient and fast; when the experiment is completed, or when the experiment is replaced midway, the toxic aquaculture wastewater in the two rows of drug solution treatment tanks can be conveniently discharged into the waste liquid recovery component for waste liquid recovery and filtration treatment, thereby removing harmful substances and preventing wastewater from polluting the environment.

[0032] In summary, the toxicity test device for zebrafish provided by the utility model can simultaneously move multiple zebrafish of the same size in a temporary holding tank to two rows or one row of drug solution treatment tanks at one time, which is convenient and fast. The time for multiple zebrafish to be placed in the drug solution can also be kept synchronized, ensuring the rigor of the test.

[0033] In a preferred embodiment, the invention further comprises: a plurality of ventilation covers, which are detachably buckled and arranged on the upper openings of the two rows of fish screens in a one-to-one correspondence. In this embodiment, a plurality of ventilation covers are provided to reduce dust pollution and reduce the volatilization of the liquid medicine.

[0034] One preferred embodiment further includes a water supply pipe II, whose water inlet is located in the temporary holding tank and whose outlet extends to the two rows of liquid treatment tanks. In this embodiment, water supply pipe II allows a portion of the temporary holding tank water to be transferred to the two rows of liquid treatment tanks, helping to maintain stable water quality in the two rows of liquid treatment tanks, utilizing beneficial substances in the temporary holding water, and preventing stress on the zebrafish.

[0035] like Figure 4 As shown, a preferred embodiment further includes: a filter box I 50, connected to the temporary holding tank via circulation line I; a pump I mounted on circulation line I; two filter boxes II 60, connected to the two rows of chemical treatment tanks via two sets of circulation line II; and two pumps II mounted on the two sets of circulation line II. In this embodiment, filter boxes I and II are used to circulate and filter impurities and excrement from the temporary holding tank and the two rows of chemical treatment tanks, keeping the water clean.

[0036] like Figure 5 、 6As shown, a preferred embodiment further includes: a bidirectional telescopic rod 70, each end of which is hingedly mounted on a pair of support frames II; and an electric telescopic rod 80, the base of which is mounted on a workbench, with the front end of the inner rod of the electric telescopic rod hingedly mounted in the middle of the bidirectional telescopic rod. By controlling the electric telescopic rod to extend and retract, the pair of support frames II can be synchronously driven to slide up and down on two sets of slide rails II. By controlling the bidirectional telescopic rod to extend and retract, the pair of support frames I can be synchronously driven to slide toward each other or toward each other on a pair of transverse rails.

[0037] In a preferred embodiment, any row of drug liquid treatment tanks includes at least three drug treatment tanks; the temporary holding tanks, the two rows of drug liquid treatment tanks and the two rows of fish screens are all made of transparent acrylic plastic material.

[0038] Although the embodiments of the present invention have been disclosed above, they are not limited to the applications listed in the description and implementation methods. They can be fully applied to various fields suitable for the present invention. For those familiar with this field, additional modifications can be easily implemented. Therefore, without departing from the general concept defined by the claims and the scope of equivalents, the present invention is not limited to the specific details and illustrations shown and described herein.

Claims

1. A toxicity test device for zebrafish, characterized in that: include: Workbench; At least one group of toxicity test components is detachably arranged on the workbench through a support frame, and any group of toxicity test components includes a temporary holding tank, which is a long strip-shaped breeding tank, and the temporary holding tank is arranged in the middle of the workbench; two rows of liquid medicine treatment tanks are sequentially arranged on both sides of the temporary holding tank, and the arrangement length of the two rows of liquid medicine treatment tanks is less than the length of the temporary holding tank, and the arrangement width of the two rows of liquid medicine treatment tanks is less than the width of the temporary holding tank; two rows of fish sieves are detachably arranged above the temporary holding tank and the two rows of liquid medicine treatment tanks through a support frame, and the two rows of fish sieves are detachably placed in the temporary holding tank, or the two rows of fish sieves are detachably placed in the two rows of liquid medicine treatment tanks in a one-to-one correspondence. The processing tank, and the specifications of any fish screen are adapted to the specifications of any liquid medicine processing tank, and the hole spacing of any fish screen is smaller than the thickness of the zebrafish body; the support frame includes support legs, which are arranged on a workbench around the two rows of liquid medicine processing tanks; a horizontal support plate, which is horizontally arranged on the support legs; a pair of slide rails I, which are arranged on the lower surface of the horizontal support plate, and the pair of slide rails I are perpendicular to the length direction of the temporary holding tank; a pair of support frames I, which are respectively slidably arranged on the pair of slide rails I; two sets of slide rails II, which are respectively arranged on the pair of support frames I, and in the vertical direction, the length of the two sets of slide rails II is greater than the height of any liquid medicine processing tank; A pair of support frames II are respectively slidably mounted on two sets of slide rails II, and two rows of fish screens are respectively detachably mounted on the pair of support frames II; a plurality of dosing bottles are respectively connected to at least two rows of liquid medicine treatment tanks via conduits I; a water supply pipe I has its outlet end extended to the temporary holding tank and the two rows of liquid medicine treatment tanks; as well as The waste liquid recovery component is arranged below the workbench and is connected to the bottoms of at least two rows of liquid treatment tanks through a waste liquid reflux pipe.

2. The toxicity test device for zebrafish according to claim 1, characterized in that: Also includes: A plurality of air-permeable covers are detachably buckled and arranged on the upper openings of the two rows of fish screens in a one-to-one corresponding manner.

3. The toxicity test device for zebrafish according to claim 1, characterized in that: Also includes: The water supply pipe II has a water inlet end arranged in the temporary holding tank, and the water outlet end of the water supply pipe II extends to two rows of liquid medicine treatment tanks respectively.

4. The toxicity test device for zebrafish according to claim 1, characterized in that: Also includes: Filter box I, which is connected to the temporary holding tank through circulation pipeline I; Pump I, which is arranged on the circulation pipeline I; Two filter boxes II are connected to two rows of liquid treatment tanks through two sets of circulation pipelines II; Two pumps II are respectively arranged on two sets of circulation pipelines II.

5. The toxicity test device for zebrafish according to claim 1, characterized in that: Also includes: A two-way telescopic rod, both ends of which are hingedly mounted on a pair of support frames II; The electric telescopic rod has a base arranged on a workbench, and the front end of the inner rod of the electric telescopic rod is hingedly arranged at the middle part of the two-way telescopic rod.

6. The toxicity test device for zebrafish according to claim 1, characterized in that: Any row of liquid medicine processing tanks includes at least three liquid medicine processing tanks; The temporary holding tank, two rows of liquid treatment tanks and two rows of fish screens are all made of transparent acrylic plastic.

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