Intensive feeding device for bactrocerae mutant insects

Abstract

The utility model discloses an intensive breeding device for fruit fly mutant insects, which comprises a breeding plate, a plurality of breeding troughs arranged on the breeding plate, and water absorbing materials loaded with nutrient solution arranged in the breeding troughs; the two ends of the feeding pipe are open, and a porous pipe plug used for sealing the opening is arranged at the opening of one end of the feeding pipe; the grid baffle is arranged on the feeding pipe, so that a feeding area in a closed state is formed in the feeding pipe under the action of the grid baffle and the porous pipe plug, and when the feeding pipe is arranged in the feeding trough, the water absorption material is connected with the grid baffle. The intensive feeding device is simple in whole device and good in air permeability, insects can be prevented from escaping in the nutrient substance supplementing and replacing process, and the feeding requirement for the tephritidae insects which suck liquid is fully met.

Description

Technical Field

[0001] The utility model relates to the technical field of insect breeding, in particular to an intensive breeding device for mutant insects of the Tephritidae family. Background Art

[0002] With the development of sequencing technology, the genomes of many common insects have been completely sequenced and annotated in detail, providing a way to gain a deeper understanding of insects. The development and application of gene editing technology has provided humans with the ability to transform and utilize these insects.

[0003] CRISPR / Cas9 technology is a gene editing system mediated by sgRNA and the Cas9 protein, specifically cleaving dsDNA and inducing gene mutations. This technology boasts universal applicability, high specificity, and high efficiency, and has been widely applied in fields such as medicine, biology, and agriculture. CRISPR / Cas9-based gene editing technology has also rapidly developed in insects, primarily for basic insect genomics research and pest control.

[0004] Genetically modified insects hold significant value and significance in both scientific and applied research. In scientific research, they provide insights into insect gene functions, developmental mechanisms, and evolutionary processes. Transgenic technology allows for precise manipulation of specific gene expression, providing a powerful tool for biological research. In applied research, they can be used for pest control. For example, mosquitoes can be bred to be disease-incapable, reducing the spread of mosquito-borne diseases. Genetically modified insects can also be used for biological monitoring, enabling rapid responses to environmental changes and providing new avenues for ecological protection and sustainable agricultural development.

[0005] Mutants obtained through methods such as embryonic microinjection often exist as mosaics, and only mutations arising in germ cells are stably inherited by offspring. Currently, the mutation rates generated by injection methods are generally low. For example, the mutation rates generated by the piggyBac transposon method in silkworms range from 3–5%, and the mutation rates generated by injection of sgRNA and Cas9 mRNA in the citrus fruit fly range from 12.1–30.2%. Therefore, it is necessary to screen for mutations in a large number of surviving individuals of the G0 generation and to obtain homozygous mutants through hybridization.

[0006] Currently, the conventional approach to creating mutant insects is to individually house all surviving G0-generation individuals after injection, mate them with wild-type individuals of the opposite sex after sexual maturity, and then test the G0-generation parents for mutations after the G1-generation individuals are produced. Alternatively, tissues from G0-generation individuals are directly selected for DNA extraction and mutation identification. Individuals identified as mutants are then reared and passaged until homozygous offspring are established. Meanwhile, raising insects in batches not only takes up a lot of experimental space, is labor-intensive, and carries the risk of insect escape during feed changes.

[0007] In view of this, it is necessary to design an intensive breeding device for mutant insects of the Tephritidae family to solve the above problems. Utility Model Content

[0008] The utility model aims to provide an intensive breeding device for mutant Tephritidae insects which has a simple structure, is easy to operate, and has good air permeability and can prevent the insects from escaping when nutrients are supplemented or replaced.

[0009] To achieve the above objectives, the present invention provides the following technical solutions:

[0010] An intensive breeding device for mutant insects of the Tephritidae family, comprising:

[0011] A feeding plate, wherein a plurality of feeding troughs are provided on the feeding plate, and a water-absorbing material loaded with a nutrient solution is provided in the feeding troughs;

[0012] A feeding tube, wherein both ends of the feeding tube are open, and a porous tube plug is provided at one end of the opening for sealing the opening;

[0013] The grid baffle is arranged on the feeding tube, so that under the action of the grid baffle and the porous tube plug, a closed feeding area is formed in the feeding tube, and when the feeding tube is placed in the feeding trough, the water-absorbing material is connected to the grid baffle.

[0014] As a further improvement of the present invention, the height of the water-absorbing material is greater than or equal to the distance from the grid baffle to the bottom surface of the feeding trough.

[0015] As a further improvement of the present invention, the grid baffle is arranged at the lower part of the feeding tube, so that when the feeding tube is placed in the feeding trough, a limited area is formed at the end of the feeding tube away from the porous pipe plug to limit the water-absorbing material to fit on the grid baffle.

[0016] As a further improvement of the present invention, the distance between the grid baffle and the bottom surface of the feeding tube is 8-12 mm.

[0017] As a further improvement of the present invention, the aperture of the grid baffle is 1-3 mm.

[0018] As a further improvement of the present invention, a plurality of air holes are provided on the upper portion of the feeding tube.

[0019] As a further improvement of the present invention, the water-absorbing material is absorbent cotton.

[0020] As a further improvement of the present invention, the porous pipe plug is a sponge plug.

[0021] As a further improvement of the present invention, the sponge plug is made of high-density latex material.

[0022] As a further improvement of the present invention, the feeding tube is made of transparent material.

[0023] The beneficial effects of the utility model are:

[0024] 1. The utility model provides openings at both ends of the feeding tube, and respectively provides a mesh baffle and a porous tube plug at the openings at both ends. The mesh baffle and the porous tube plug can be used to form a closed feeding area in the feeding tube. At the same time, when the feeding tube is placed on the feeding plate, the water-absorbing material contacts the mesh baffle, so that the insects can absorb the nutrient solution through the water-absorbing material located on the mesh baffle. This ensures that the insects can stably absorb nutrients while increasing the convenience of replacing the liquid artificial feed of the Tephritidae family, that is, the nutrient solution.

[0025] 2. The utility model provides a mesh baffle at one end of the feeding tube and a porous tube plug for sealing at the other end. When the feeding tube is removed from the feeding plate to replenish nutrients, the gas flow inside and outside the feeding tube is enhanced, thereby fully meeting the insects' demand for oxygen.

[0026] 3. The intensive breeding device of the present invention can breed Tephritidae insects individually, making the breeding conditions uniform and precise.

[0027] 4. The intensive breeding device of the present invention is simple in overall structure and has good air permeability, which can prevent insects from escaping during the process of supplementing and replacing nutrients, and fully meets the breeding needs of Tephritidae insects that feed on liquids. BRIEF DESCRIPTION OF THE DRAWINGS

[0028] Figure 1 The utility model is a schematic structural diagram of the intensive breeding device for mutant insects of the Tephritidae family.

[0029] Figure 2 Schematic diagram of the internal structure of the feeding tube.

[0030] Figure 3 Schematic diagram of the position of the grid baffle and the water-absorbing material.

[0031] Reference numerals

[0032] 10. Feeding board; 11. Feeding trough; 12. Enclosure; 20. Feeding tube; 21. Porous pipe plug; 22. Mesh baffle; 23. Air vent; 30. Defined area; 40. Water-absorbing material. DETAILED DESCRIPTION

[0033] In order to make the purpose, technical solutions and advantages of the present invention more clear, the present invention is described in detail below with reference to the accompanying drawings and specific embodiments.

[0034] It should also be noted here that, in order to avoid obscuring the present invention due to unnecessary details, only structures and / or processing steps closely related to the scheme of the present invention are shown in the accompanying drawings, while other details that are not closely related to the present invention are omitted.

[0035] In addition, it should be noted that the terms "comprises", "includes" or any other variations thereof are intended to cover non-exclusive inclusion, so that a process, method, article or apparatus that includes a series of elements includes not only those elements, but also includes other elements not explicitly listed, or also includes elements inherent to such process, method, article or apparatus.

[0036] See also Figure 1-Figure 3 As shown, the utility model provides an intensive breeding device for mutant insects of the Tephritidae family, comprising:

[0037] A feeding plate 10 is provided with a plurality of feeding troughs 11, and a water-absorbing material 40 loaded with a nutrient solution is provided in the feeding troughs 11;

[0038] A feeding tube 20, wherein both ends of the feeding tube 20 are open, and a porous pipe plug 21 is provided at one end of the opening for sealing the opening;

[0039] The mesh baffle 22 is provided on the feeding tube 20 , so that under the action of the mesh baffle 22 and the porous pipe plug 21 , a closed feeding area is formed in the feeding tube 20 , and when the feeding tube 20 is placed in the feeding trough 11 , the water-absorbing material 40 is in contact with the mesh baffle 22 .

[0040] Exemplarily, the feeding plate 10 is rectangular, and a plurality of grids, namely feeding troughs 11, are provided in the feeding plate 10.

[0041] For example, the length, width and height of the feeding plate 10 are 255 mm, 255 mm and 25 mm respectively, and 10×10 squares are provided on the feeding plate 10, and the square size is 25 mm×25 mm; the feeding tube 20 has a diameter of 24 mm and a height of 100 mm, so that the feeding tube 20 can be accurately and stably inserted into the square, realizing the intensive management of mutant insects of the Tephritidae family.

[0042] The feeding plate 10 is further surrounded by a baffle plate 12 with a height of 10 mm.

[0043] Specifically, the height of the water-absorbing material 40 is greater than or equal to the distance from the mesh baffle 22 to the bottom surface of the feeding trough 11, so that when the feeding tube 20 is placed in the feeding trough 11, the water-absorbing material 40 contacts the mesh baffle 22, thereby facilitating the insects to absorb the nutrient solution through the water-absorbing material 40 located on the mesh baffle 22 ( Figure 3 ).

[0044] Exemplarily, the water-absorbing material 40 is absorbent cotton;

[0045] The preparation of liquid artificial feed, i.e. nutrient solution, for Tephritidae is as follows: in a 500ml standard beaker, accurately weigh 10g of yeast extract powder and 30g of sucrose, then add about 100ml of ddH2O, stir evenly with a glass rod and adjust the volume to 300ml. Place the prepared feed solution in a 121℃ high pressure sterilizer for 20min for sterilization, and then store in a 4℃ refrigerator for use.

[0046] The absorbent cotton is fully soaked in the nutrient solution so that the absorbent cotton absorbs enough nutrient solution, thereby preparing the water-absorbing material 40 loaded with the nutrient solution.

[0047] Specifically, the mesh baffle 22 is disposed at the end of the feeding tube 20 away from the porous tube plug 21, i.e., at the lower portion of the feeding tube 20. Exemplarily, the distance between the mesh baffle 22 and the bottom surface of the feeding tube 20 is 8-12 mm. Since the bottom surface of the feeding tube 20 is open, when the feeding tube 20 is placed in the feeding trough 11, a defined area 30 is formed between the mesh baffle 22, the tube wall of the feeding tube 20, and the bottom surface of the feeding plate 10. When the feeding tube 20 is placed in the feeding trough 11, the water-absorbing material 40 is sheathed within the defined area 30, with the top surface of the water-absorbing material 40 in contact with the mesh baffle 22, or a portion of the water-absorbing material 40 extends beyond the mesh of the mesh baffle 22 into the feeding tube 20. This allows insects in the feeding tube 20 to obtain nutrients by absorbing the nutrient solution on the water-absorbing material 40 that has passed through the mesh baffle 22.

[0048] Exemplarily, the pore size of the mesh baffle 22 is 1-3 mm.

[0049] Specifically, the feeding tube 20 is made of a transparent material to facilitate observation of the living conditions of the insects in the tube.

[0050] Specifically, the porous tube plug 21 is a sponge plug made of high-density latex. This allows the porous tube plug 21 to effectively seal the top opening of the feeding tube 20, preventing the fruit fly from escaping through the top while ensuring air permeability and maintaining normal air circulation within the feeding tube 20. Furthermore, when the feeding tube 20 is removed from the feeding plate 10 for nutritional supplementation, the mesh baffles 22 and the porous tube plug 21 at either end of the feeding tube 20 provide excellent air permeability, thereby enhancing air flow inside and outside the feeding tube 20 and fully meeting the insects' oxygen needs.

[0051] Specifically, a plurality of air holes 23 are further provided on the upper portion of the feeding tube 20 to meet the feeding needs of some insects that require higher air permeability.

[0052] During feeding, anesthetized insects are placed in a feeding tube 20, and the top of the feeding tube 20 is immediately sealed with a porous pipe plug 21. At the same time, a water-absorbing material 40 loaded with nutrient solution is placed in the feeding tank 11. The feeding tube 20 is then inserted into the feeding tank 11, so that the water-absorbing material 40 is tightly fitted with the mesh baffle 22, so that the insects can ingest the nutrient solution in the water-absorbing material 40 through the mesh baffle 22 to maintain their survival. After 1-2 days, the feeding tube 20 is removed and replaced with a new water-absorbing material 40 loaded with nutrient solution.

[0053] The above embodiments are only used to illustrate the technical solutions of the present invention and are not intended to limit the present invention. Although the present invention has been described in detail with reference to the preferred embodiments, those skilled in the art should understand that the technical solutions of the present invention may be modified or replaced by equivalents without departing from the spirit and scope of the technical solutions of the present invention.

Claims

1. An intensive breeding device for mutant insects of the Tephritidae family, characterized in that: include: A feeding plate, wherein a plurality of feeding troughs are provided on the feeding plate, and a water-absorbing material loaded with a nutrient solution is provided in the feeding troughs; A feeding tube, wherein both ends of the feeding tube are open, and a porous tube plug is provided at one end of the opening for sealing the opening; The grid baffle is arranged on the feeding tube, so that under the action of the grid baffle and the porous tube plug, a closed feeding area is formed in the feeding tube, and when the feeding tube is placed in the feeding trough, the water-absorbing material is connected to the grid baffle.

2. The intensive breeding device for mutant Tephritidae insects according to claim 1, characterized in that: The height of the water-absorbing material is greater than or equal to the distance from the grid baffle to the bottom surface of the feeding trough.

3. The intensive breeding device for mutant Tephritidae insects according to claim 1, characterized in that: The grid baffle is arranged at the lower part of the feeding tube, so that when the feeding tube is placed in the feeding trough, a limited area is formed at the end of the feeding tube away from the porous pipe plug to limit the water absorbing material to fit on the grid baffle.

4. The intensive breeding device for mutant Tephritidae insects according to claim 3, characterized in that: The distance between the grid baffle and the bottom surface of the feeding tube is 8-12 mm.

5. The intensive breeding device for mutant Tephritidae insects according to claim 1, characterized in that: The aperture of the grid baffle is 1-3 mm.

6. The intensive breeding device for mutant Tephritidae insects according to claim 1, characterized in that: The upper part of the feeding tube is provided with a plurality of air holes.

7. The intensive breeding device for mutant Tephritidae insects according to claim 1, characterized in that: The water-absorbing material is absorbent cotton.

8. The intensive breeding device for mutant Tephritidae insects according to claim 1, characterized in that: The porous pipe plug is a sponge plug.

9. The intensive breeding device for mutant Tephritidae insects according to claim 8, characterized in that: The sponge plug is made of high-density latex material.

10. The intensive breeding device for mutant Tephritidae insects according to claim 1, characterized in that: The feeding tube is made of transparent material.

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