Device for stripping envelope in culture process of primary spinal cord neurons
By designing a device consisting of a base, a microscope, a water channel and a non-slip pad, the problem of ordinary ice packs affecting the transmittance of the microscope was solved, and the spinal cord membrane was removed efficiently and stably, ensuring the cell purity and clarity of the microscope.
Patent Information
- Application Number
- CN202422446286.8
- Authority / Receiving Office
- CN · China
- Patent Type
- Utility models(China)
- Current Assignee / Owner
- Filing Date
- 2024-10-10
- Publication Date
- 2025-09-26
- Estimated Expiration
- 2034-10-10
AI Technical Summary
In the prior art, when ordinary ice packs are used to peel off the spinal cord membrane, the light transmittance of the microscope is affected, resulting in incomplete membrane peeling, affecting cell purity and low efficiency.
A device was designed, including a base, a microscope, a water channel, a round table, and a culture dish. Ice cubes were made using a -20-degree Celsius refrigerator to ensure good light transmittance of the microscope. Melted water was drained through through holes and water channels. The height was adjusted using anti-slip pads and threaded tubes to ensure the stability of the device on an uneven table.
It achieves efficient stripping of the spinal cord membrane at low temperature, ensuring cell purity and microscope clarity, while improving the convenience and stability of operation.
Smart Images
Figure CN223386135U_ABST
Abstract
Description
Technical Field
[0001] The utility model relates to the technical field of traction supports, in particular to a device for stripping off the membrane during the culture process of primary spinal cord neurons. Background Art
[0002] The spinal cord is covered with three layers: the dura mater, the arachnoid mater, and the pia mater. Microscopically stripping the spinal cord membranes must be performed on ice and quickly to prevent liquefaction of the spinal cord. Conventional dissecting microscopes project light from below, but stripping requires both sufficient light and ice. Therefore, a device is needed to meet these two requirements.
[0003] Currently, there is no unified and perfect device for removing the spinal cord membrane. The use of ordinary ice packs will affect the light transmittance of the microscope, resulting in incomplete membrane removal and serious impact on cell purity. In addition, the device is inefficient and not conducive to its widespread use.
[0004] Therefore, the utility model provides a device for stripping the membrane during the culture process of primary spinal cord neurons. Utility Model Content
[0005] The purpose of the utility model is to solve the shortcomings in the prior art that the use of ordinary ice bags will affect the transmittance of the microscope, resulting in unclean membrane stripping and seriously affecting the purity of cells. A device for stripping the membrane during the culture of primary spinal cord neurons is proposed.
[0006] In order to achieve the above-mentioned purpose, the present invention adopts the following technical solution: a device for stripping the membrane during the culture of primary spinal cord neurons, comprising a base, a microscope fixedly mounted on the top of the base, a water guide groove provided on the outside of the base, a through hole provided on the inner wall of the water guide groove, a placement groove provided on the top of the base, a round table fixedly mounted on the inner wall of the placement groove, a disc fixedly mounted on the inner wall of the placement groove, a culture dish provided on the top of the disc, ice cubes provided on the inner wall of the culture dish, and a sealing cover provided on the top of the culture dish.
[0007] Preferably, four threaded tubes are fixedly mounted on the bottom of the base, and the inner walls of the four threaded tubes are all threadedly connected with anti-slip pads.
[0008] Preferably, a circular groove matching the diameter of the frustum is opened in the middle of the disc, and the frustum is arranged on the inner wall of the circular groove.
[0009] Preferably, a notch is provided on the top of the microscope, and the through hole is communicated with the inner wall of the placement groove.
[0010] Preferably, a handle is fixedly installed on the top of the sealing cover, the culture dish and the sealing cover are both made of glass, and the ice cube is set below the microscope.
[0011] Preferably, the outer edge of the anti-slip pad is evenly provided with a plurality of protrusions, and the anti-slip pad is made of rubber.
[0012] In summary:
[0013] 1. In the present invention, a culture dish filled with water is placed in a refrigerator at -20 degrees Celsius until it is completely solidified to form ice cubes. The culture dish can then be placed on top of the disc. The culture dish containing the spinal cord tissue can then be placed on top of the ice cubes, thereby ensuring perfect light transmission, ensuring the efficiency of the membrane peeling, and ensuring that the tissue will not liquefy under low temperature conditions.
[0014] 2. In the utility model, the water that seeps out after the ice melts when the film is peeled off can be discharged to the outside through the through hole and the water guide groove. The user can use his fingers to press the disc to take it out to the outside through the notch, thereby facilitating the disc to be taken out to the outside for cleaning and maintenance. The distance between the anti-slip pad and the threaded tube can be adjusted by rotating the anti-slip pad, thereby facilitating fine adjustment of the height of the base, and the four anti-slip pads can be adjusted to not be at the same height, thereby ensuring stable placement on an uneven table. BRIEF DESCRIPTION OF THE DRAWINGS
[0015] Figure 1 It is a schematic diagram of the three-dimensional structure of the utility model;
[0016] Figure 2 It is a schematic diagram of the local structure of the utility model;
[0017] Figure 3 For the utility model Figure 2 A in the middle is an enlarged structural diagram;
[0018] Figure 4 It is a schematic diagram of the expanded structure of the utility model.
[0019] Legend: 1. Base; 2. Microscope; 3. Threaded tube; 4. Anti-slip pad; 5. Protrusion; 6. Water guide groove; 7. Through hole; 8. Mounting slot; 9. Round table; 10. Round plate; 11. Notch; 12. Petri dish; 13. Ice cube; 14. Sealing cover; 15. Handle. DETAILED DESCRIPTION
[0020] Reference Figure 1-Figure 4As shown, the utility model provides a technical solution: a device for stripping the membrane during the culture process of primary spinal cord neurons, comprising a base 1, a microscope 2 is fixedly installed on the top of the base 1, a water guide groove 6 is provided on the outside of the base 1, a through hole 7 is provided on the inner wall of the water guide groove 6, a placement groove 8 is provided on the top of the base 1, a round table 9 is fixedly installed on the inner wall of the placement groove 8, a disc 10 is fixedly installed on the inner wall of the placement groove 8, a culture dish 12 is provided on the top of the disc 10, an ice cube 13 is provided on the inner wall of the culture dish 12, and a sealing cover 14 is provided on the top of the culture dish 12.
[0021] Reference Figure 2 As shown, in this embodiment: four threaded tubes 3 are fixedly installed on the bottom of the base 1, and the inner walls of the four threaded tubes 3 are threadedly connected with anti-slip pads 4, so that the anti-slip pads 4 can be rotated to adjust the distance between the anti-slip pads 4 and the threaded tubes 3, thereby facilitating fine-tuning the height of the base 1, and the four anti-slip pads 4 can be adjusted to not be at the same height, thereby ensuring stable placement on an uneven table top.
[0022] Reference Figure 4 As shown, in this embodiment: a circular groove adapted to the diameter of the cone 9 is opened in the middle of the disc 10, and the cone 9 is arranged on the inner wall of the circular groove, so that the disc 10 can be positioned to ensure that the disc 10 can be stably set on the inner wall position of the placement groove 8 to ensure that it will not shake easily.
[0023] Reference Figure 4 As shown, in this embodiment: a notch 11 is provided on the top of the microscope 2, and the through hole 7 is communicated with the inner wall of the placement groove 8, so that the user can easily press the disc 10 with his fingers to take it out to the outside, and then it is convenient to take the disc 10 out to the outside for cleaning and maintenance. The through hole 7 is communicated with the inner wall of the placement groove 8, so that the water that seeps out after the ice cube 13 melts during operation can be discharged to the outside through the through hole 7 and the water guide groove 6 without accumulating on the inner wall of the placement groove 8.
[0024] Reference Figure 4 As shown, in this embodiment: a handle 15 is fixedly installed on the top of the sealing cover 14, the culture dish 12 and the sealing cover 14 are both made of glass, and the ice cube 13 is set below the microscope 2. The handle 15 increases the convenience of the user in taking off the sealing cover 14. Since the culture dish 12 and the sealing cover 14 are both made of glass, the culture dish 12 containing the spinal cord tissue can be placed on the top of the device, so that it can be perfectly transparent, ensuring the efficiency of the membrane stripping and ensuring that the tissue will not liquefy under low temperature conditions.
[0025] Reference Figure 2As shown, in this embodiment: the outer edge of the anti-slip pad 4 is evenly provided with a plurality of protrusions 5, and the anti-slip pad 4 is made of rubber, which increases the convenience of rotating the anti-slip pad 4 and improves the friction of the hand when rotating the anti-slip pad 4.
[0026] Working principle: when using this device, first fill the culture dish 12 with water and place it in a refrigerator at minus 20 degrees Celsius until it is completely solidified to form ice cubes 13, then the culture dish 12 can be placed on the top position of the disc 10, and then the culture dish 12 containing the spinal cord tissue can be placed on top of the ice cubes 13, so that it can be perfectly transparent, ensuring the efficiency of peeling the membrane, and ensuring that the tissue will not liquefy under low temperature conditions, ensuring clarity when viewed through the microscope 2, and when peeling the membrane, the water that seeps out after the ice cube 13 melts can be discharged to the outside through the through hole 7 and the water guide groove 6, and will not accumulate on the inner wall of the placement groove 8. The notch 11 makes it convenient for the user to use his fingers to press the disc 10 to take it out to the outside, and then it is convenient to take the disc 10 out to the outside for cleaning and maintenance. By rotating the anti-slip pad 4, the distance between the anti-slip pad 4 and the threaded tube 3 can be adjusted, which facilitates fine-tuning of the height of the base 1, and the four anti-slip pads 4 can be adjusted to not be at the same height, thereby ensuring stable placement on an uneven table.
[0027] In the description of this utility model, it should be noted that, unless otherwise expressly specified or limited, the terms "installed," "connected," and "connected" should be understood in a broad sense. For example, they can refer to fixed connections, detachable connections, or integral connections; mechanical connections, electrical connections; direct connections, indirect connections through an intermediate medium, and internal communication between two components. Those skilled in the art will understand the specific meanings of the above terms in this utility model based on specific circumstances.
Claims
1. A device for removing the membrane during the culture of primary spinal cord neurons, comprising a base (1), characterized in that: A microscope (2) is fixedly mounted on the top of the base (1), a water guide groove (6) is provided on the outer side of the base (1), a through hole (7) is provided on the inner wall of the water guide groove (6), a placement groove (8) is provided on the top of the base (1), a round table (9) is fixedly mounted on the inner wall of the placement groove (8), a disc (10) is fixedly mounted on the inner wall of the placement groove (8), a culture dish (12) is provided on the top of the disc (10), an ice cube (13) is provided on the inner wall of the culture dish (12), and a sealing cover (14) is provided on the top of the culture dish (12).
2. The device for removing the membrane during the culture of primary spinal cord neurons according to claim 1, characterized in that: Four threaded tubes (3) are fixedly mounted on the bottom of the base (1), and the inner walls of the four threaded tubes (3) are all threadedly connected with anti-slip pads (4).
3. The device for removing the membrane during the culture of primary spinal cord neurons according to claim 1, characterized in that: A circular groove matching the diameter of the truncated cone (9) is provided in the middle of the circular disc (10), and the truncated cone (9) is arranged on the inner wall of the circular groove.
4. The device for removing the membrane during the culture of primary spinal cord neurons according to claim 1, characterized in that: A notch (11) is provided on the top of the microscope (2), and the through hole (7) is communicated with the inner wall of the placement groove (8).
5. The device for removing the membrane during the culture of primary spinal cord neurons according to claim 1, characterized in that: A handle (15) is fixedly mounted on the top of the sealing cover (14); the culture dish (12) and the sealing cover (14) are both made of glass; and the ice cube (13) is disposed below the microscope (2).
6. The device for removing the membrane during the culture of primary spinal cord neurons according to claim 2, characterized in that: The outer edge of the anti-slip pad (4) is evenly provided with a plurality of protrusions (5), and the anti-slip pad (4) is made of rubber.