Separation and purification device for polyglutamic acid fermentation liquor

Abstract

The utility model discloses a polyglutamic acid fermentation liquor separation and purification device which comprises a base, and a pretreatment tank, a filtering tank and a controller are arranged on the base; a cover body is detachably arranged on the pretreatment tank, and a dilution assembly is arranged in the pretreatment tank; a storage tank is arranged below the pretreatment tank, and the upper end of the storage tank is through; a separation assembly is arranged between the pretreatment tank and the storage tank; a conveying pipe is arranged between the lower end of the storage tank and the upper end of the filtering tank, and a pressurizing pipe is arranged at the upper end of the filtering tank; a grating frame is arranged in the filtering tank, a micro-filtration membrane is arranged on the outer side of the grating frame, and the conveying pipe and the pressurizing pipe both extend into the micro-filtration membrane. Before separation and filtration, dilution is performed firstly, the viscosity of fermentation liquor is reduced by adopting high-concentration potassium chloride, then solid-liquid separation is performed, solid impurities are separated out firstly, and the fermentation liquor with reduced viscosity is not easy to adhere to a stirring structure; thalli and macromolecular impurities in the fermentation liquor are removed through a micro-filtration membrane, so that the purification effect is improved.

Description

Technical Field

[0001] The utility model relates to the technical field of biological polymer material processing, in particular to a polyglutamic acid fermentation liquid separation and purification device. Background Art

[0002] Polyglutamic acid is a water-soluble, biodegradable, non-toxic biopolymer. Due to its environmentally friendly and naturally non-toxic properties, it has shown broad application potential in many fields. In addition to its wide application in agriculture, it is also used in cosmetics, food grade, pharmaceutical grade, water treatment grade, medical and health care and other technical fields.

[0003] Polyglutamic acid fermentation broth can improve soil physical and chemical properties, including water retention, water permeability, and air permeability, balance soil pH, improve poor-quality soils, increase crop yields, and maintain sustainable development. Due to the complex metabolic pathways involved in polyglutamic acid fermentation using microorganisms, the fermentation broth becomes viscous. This high viscosity significantly reduces dissolved oxygen concentration and greatly increases mass transfer resistance, making oxygen supply difficult, preventing the effective utilization of nutrients, and inhibiting bacterial growth and metabolism.

[0004] Patent application number CN202321989831.7 and publication (announcement) number CN220736619U discloses a polyglutamic acid fermentation broth separation and purification device that utilizes a purification tank, a first filter plate, and a second filter plate to perform purification. However, in the technical solution of the above patent, due to the extremely high viscosity of the polyglutamic acid fermentation broth, the stirring rod is difficult to achieve normal stirring, and the fermentation broth may adhere to the stirring rod, resulting in poor stirring effect. The fermentation broth has poor fluidity, and using a simple filter plate is difficult to achieve the required separation effect, and the filter plate may become clogged and difficult to clean. In summary, there is room for improvement. Utility Model Content

[0005] The utility model aims to solve the above-mentioned technical problem of poor separation and purification effect of polyglutamic acid fermentation broth in the prior art, and provides a polyglutamic acid fermentation broth separation and purification device.

[0006] In order to solve the above technical problems, the technical solution provided by the present invention is as follows: a polyglutamic acid fermentation liquid separation and purification device, comprising a base, on which a pretreatment tank, a filter tank, and a controller are provided;

[0007] The pretreatment tank is provided with a detachable cover, and a dilution assembly is provided in the pretreatment tank, the dilution assembly including a fermentation liquid tube and a reagent tube respectively provided on both sides of the pretreatment tank and near the upper end; an inner tank is provided in the pretreatment tank, the lower end of the inner tank is funnel-shaped, and a solenoid valve is provided at the lower end; a motor is provided at the upper end of the cover, and the output end of the motor extends into the inner tank and is provided with a stirring structure;

[0008] The lower end of the pretreatment tank is penetrated, and a storage tank is provided below, and the upper end of the storage tank is penetrated; a separation assembly is provided between the pretreatment tank and the storage tank; the separation assembly includes an adjustment plate rotatably connected to the pretreatment tank and the storage tank, and the adjustment plate is provided with a plurality of through holes 1; the lower end of the pretreatment tank is provided with a bottom plate, and the bottom plate is provided with through holes 2 corresponding to the through holes 1;

[0009] A delivery pipe is provided between the lower end of the storage tank and the upper end of the filter tank, a pressure pipe is provided at the upper end of the filter tank, and an output pipe is provided at the lower end; a grid frame is provided inside the filter tank, a microfiltration membrane is provided outside the grid frame, and the delivery pipe and the pressure pipe both extend into the microfiltration membrane.

[0010] Furthermore, flanges are provided on the outer side of the upper end of the pretreatment tank and the outer side of the lower end of the cover, and the flanges are connected by a plurality of bolts.

[0011] Furthermore, the fermentation liquid tube and the reagent tube both extend into the inner tank.

[0012] Furthermore, the stirring structure includes a rotating rod extending from the output end of the motor into the inner tank, and a plurality of stirring rods are provided on the outside of the rotating rod.

[0013] Furthermore, a plurality of fixing frames are connected between the pretreatment tank and the outer side of the storage tank, and the fixing frames are all U-shaped.

[0014] Furthermore, the upper and lower ends of the adjustment plate are provided with limiting rings, the lower end of the pretreatment tank and the upper end of the storage tank are provided with circular grooves, and the circular grooves are provided with sealed bearings that can be rotatably connected to the limiting rings.

[0015] Furthermore, an auxiliary ring is provided on the outer side of the adjustment plate through a plurality of support rods; one of the support rods is provided with a marking arrow 1, and the outer side of the pretreatment tank is provided with a marking arrow 2.

[0016] The advantages of this utility model compared with the prior art are:

[0017] Before separation and filtration, dilution is first performed, and high concentration potassium chloride is used to reduce the viscosity of the fermentation liquid to improve its fluidity. Then solid-liquid separation is performed to first separate solid impurities. The fermentation liquid with reduced viscosity is not easy to adhere to the stirring structure.

[0018] The microfiltration membrane is used to remove bacteria and macromolecular impurities in the fermentation broth to improve the purification effect. BRIEF DESCRIPTION OF THE DRAWINGS

[0019] Figure 1 It is a structural diagram of the present utility model.

[0020] Figure 2It is a main perspective view of the pretreatment tank and storage tank of the utility model.

[0021] Figure 3 It is a schematic diagram of the separation component structure of the utility model.

[0022] Figure 4 This is a main perspective view of the filter tank of the present invention.

[0023] As shown in the figure: 1. Base, 2. Pretreatment tank, 3. Filter tank, 4. Controller, 5. Cover, 6. Flange, 7. Fermentation liquid tube, 8. Reagent tube, 9. Inner tank, 10. Solenoid valve, 11. Motor, 12. Rotating rod, 13. Stirring rod, 14. Storage tank, 15. Fixed bracket, 16. Adjustment plate, 17. Through hole 1, 18. Bottom plate, 19. Through hole 2, 20. Limiting ring, 21. Circular groove, 22. Auxiliary ring, 23. Marking arrow 1, 24. Marking arrow 2, 25. Delivery pipe, 26. Pressurized pipe, 27. Output pipe, 28. Microfiltration membrane. DETAILED DESCRIPTION

[0024] The present invention will be described in further detail below with reference to the accompanying drawings.

[0025] Example 1, combined with the attached Figure 1 A polyglutamic acid fermentation liquid separation and purification device includes a base 1, on which a pretreatment tank 2, a filter tank 3, and a controller 4 are provided; the controller 4 controls the start and stop of energized components such as a solenoid valve 10;

[0026] The pretreatment tank 2 is provided with a detachable cover 5. Flanges 6 are provided on the outer side of the upper end of the pretreatment tank 2 and the outer side of the lower end of the cover 5. The flanges 6 are connected by several bolts, so that the cover 5 can be separated from the pretreatment tank 2 and the stirring structure can be taken out as a whole for easy cleaning.

[0027] Combined with attachment Figure 1 、 2 A dilution component is provided in the pretreatment tank 2, and the dilution component includes a fermentation liquid pipe 7 and a reagent pipe 8 respectively provided on both sides of the pretreatment tank 2 and near the upper end; an inner tank 9 is provided in the pretreatment tank 2, and the fermentation liquid pipe 7 and the reagent pipe 8 both extend into the inner tank 9; the reagent pipe 8 transports the potassium chloride solution for dilution;

[0028] The lower end of the inner tank 9 is funnel-shaped and is provided with a solenoid valve 10 to control the transmission of the diluted fermentation liquid; the upper end of the cover body 5 is provided with a motor 11, and the output end of the motor 11 extends into the inner tank 9 to provide a stirring structure; the stirring structure includes a rotating rod 12 extending from the output end of the motor 11 into the inner tank 9, and a plurality of stirring rods 13 are provided on the outside of the rotating rod 12, which can stir during the dilution process to improve uniformity;

[0029] Combined with attachment Figure 1The lower end of the pretreatment tank 2 is penetrated, and a storage tank 14 is provided below, and the upper end of the storage tank 14 is penetrated; a number of fixing frames 15 are connected between the outside of the pretreatment tank 2 and the storage tank 14, and the fixing frames 15 are all U-shaped, so that the pretreatment tank 2 and the storage tank 14 are relatively fixed, and will not rotate with the rotation of the adjusting plate 16 in the middle.

[0030] Combined with attachment Figure 1 、 2 3. A separation assembly is provided between the pretreatment tank 2 and the storage tank 14; the separation assembly includes an adjustment plate 16 rotatably connected to the pretreatment tank 2 and the storage tank 4, and a limit ring 20 is provided at the upper and lower ends of the adjustment plate 16. A circular groove 21 is provided at the lower end of the pretreatment tank 2 and the upper end of the storage tank 14, and a sealed bearing that can be rotatably connected to the limit ring 20 is provided in the circular groove, so that the adjustment plate 16 can rotate freely between the pretreatment tank 2 and the storage tank 14;

[0031] The adjustment plate 16 is provided with a plurality of through holes 17; a bottom plate 18 is provided at the lower end of the pretreatment tank 2, and a through hole 19 corresponding to the through hole 17 is provided on the bottom plate 18; by rotating the adjustment plate 16, the overlapping area between the through hole 17 and the through hole 19 can be adjusted, thereby adjusting the volume of impurities that can be intercepted, and can be adjusted according to actual use needs; in addition, the device is applicable to other liquid separation and purification.

[0032] Combined with attachment Figure 1 、 3 An auxiliary ring 22 is provided on the outside of the adjustment plate 16 through a plurality of support rods; a marking arrow 1 23 is provided on one of the support rods, and a marking arrow 2 24 is provided on the outside of the pretreatment tank 2. In the initial state, that is, when the through hole 17 and the through hole 2 19 completely overlap, the marking arrow 1 23 corresponds to the marking arrow 2 24, so that the overlapping state of the through hole 17 and the through hole 2 19 can be judged by the amount of deviation between the two.

[0033] Combined with attachment Figure 1 、 4 A delivery pipe 25 is provided between the lower end of the storage tank 14 and the upper end of the filter tank 3, which can introduce the diluted and filtered fermentation liquid into the filter tank 3. A pressurizing pipe 26 is provided at the upper end of the filter tank 3. A grid frame is provided inside the filter tank 3, and a microfiltration membrane 28 is provided on the outside of the grid frame. The delivery pipe 25 and the pressurizing pipe 26 both extend into the microfiltration membrane 28, which can increase the fermentation liquid and speed up the fermentation liquid passing through the microfiltration membrane 28; an output pipe 27 is provided at the lower end to output the fermentation liquid after microfiltration.

[0034] The working principle of the present invention is as follows: the fermentation liquid first enters the inner tank 9 through the fermentation liquid pipe 7, and high-concentration potassium chloride is added to the inner tank 9 from the reagent tube 8 for dilution. During this process, the motor 11 drives the rotating rod 12 and the stirring rod 13 to stir and promote dilution; the adjustment plate 16 is rotated by the auxiliary ring 22 to adjust the overlapping area between the through hole 17 and the through hole 2 19, so as to adjust the volume of impurities that can be intercepted during filtration; the solenoid valve 10 is opened to release the diluted fermentation liquid for solid-liquid separation, enters the storage tank 14, and then is transferred to the filter tank 3 through the delivery pipe 25, and then microfiltered through the microfiltration membrane 28 to intercept large molecular impurities.

[0035] The above description of the present invention and its embodiments is non-limiting. The drawings show only one embodiment of the present invention, and the actual structure is not limited thereto. In short, if a person skilled in the art is inspired by the above, and does not deviate from the purpose of the present invention, without inventive design, a structure and embodiment similar to the technical solution should fall within the scope of protection of the present invention.

Claims

1. A polyglutamic acid fermentation broth separation and purification device, characterized in that: It comprises a base (1), on which a pretreatment tank (2), a filter tank (3), and a controller (4) are provided; The pretreatment tank (2) is provided with a detachable cover (5), and a dilution assembly is provided in the pretreatment tank (2), and the dilution assembly includes a fermentation liquid tube (7) and a reagent tube (8) respectively provided on both sides of the pretreatment tank (2) and near the upper end; an inner tank (9) is provided in the pretreatment tank (2), and the lower end of the inner tank (9) is funnel-shaped and is provided with a solenoid valve (10); a motor (11) is provided at the upper end of the cover (5), and the output end of the motor (11) extends into the inner tank (9) and is provided with a stirring structure; The lower end of the pretreatment tank (2) is penetrated, and a storage tank (14) is provided below, and the upper end of the storage tank (14) is penetrated; a separation assembly is provided between the pretreatment tank (2) and the storage tank (14); the separation assembly includes an adjustment plate (16) rotatably connected to the pretreatment tank (2) and the storage tank (14), and a plurality of through holes (17) are arranged on the adjustment plate (16); a bottom plate (18) is provided at the lower end of the pretreatment tank (2), and a through hole (19) corresponding to the through hole (17) is arranged on the bottom plate (18); A delivery pipe (25) is provided between the lower end of the storage tank (14) and the upper end of the filter tank (3); a pressure pipe (26) is provided at the upper end of the filter tank (3), and an output pipe (27) is provided at the lower end; a grid frame is provided inside the filter tank (3), and a microfiltration membrane (28) is provided outside the grid frame; the delivery pipe (25) and the pressure pipe (26) both extend into the microfiltration membrane (28).

2. A polyglutamic acid fermentation broth separation and purification device according to claim 1, characterized in that: The outer side of the upper end of the pretreatment tank (2) and the outer side of the lower end of the cover (5) are both provided with flanges (6), and the flanges (6) are connected by a plurality of bolts.

3. A polyglutamic acid fermentation broth separation and purification device according to claim 1, characterized in that: The fermentation liquid tube (7) and the reagent tube (8) both extend into the inner tank (9).

4. A polyglutamic acid fermentation broth separation and purification device according to claim 1, characterized in that: The stirring structure comprises a rotating rod (12) provided at the output end of the motor (11) extending into the inner tank (9), and a plurality of stirring rods (13) are provided on the outer side of the rotating rod (12).

5. A polyglutamic acid fermentation broth separation and purification device according to claim 1, characterized in that: A plurality of fixing frames (15) are connected between the pretreatment tank (2) and the outer side of the storage tank (14), and the fixing frames (15) are all U-shaped.

6. A polyglutamic acid fermentation broth separation and purification device according to claim 1, characterized in that: The upper and lower ends of the adjustment plate (16) are both provided with limiting rings (20), the lower end of the pretreatment tank (2) and the upper end of the storage tank (14) are both provided with circular grooves (21), and the circular grooves are both provided with sealed bearings that can be rotatably connected to the limiting rings (20).

7. A polyglutamic acid fermentation broth separation and purification device according to claim 1, characterized in that: An auxiliary ring (22) is provided on the outside of the adjustment plate (16) via a plurality of support rods; one of the support rods is provided with a marking arrow 1 (23), and the outside of the pretreatment tank (2) is provided with a marking arrow 2 (24).

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