Immobilized enzyme reaction system

Abstract

The utility model relates to the technical field of enzyme reaction, in particular to an immobilized enzyme reaction system which comprises a material mixing tank, the material mixing tank is connected with a first conveying pipe, an outlet of the first conveying pipe is connected with a triple hollow fiber column reactor, an outlet of the triple hollow fiber column reactor is connected with a second conveying pipe, and the second conveying pipe is connected with a third conveying pipe. An outlet of the second conveying pipe is communicated with a crystallizing tank; the triple hollow fiber column reactor comprises a first hollow fiber column reactor, a second hollow fiber column reactor and a third hollow fiber column reactor, and the first hollow fiber column reactor, the second hollow fiber column reactor and the third hollow fiber column reactor are connected in series through guide pipes. Through the arrangement of the material mixing tank, the crystallizing tank and the triple hollow fiber column reactor, the problems of low reaction efficiency and poor stability of an immobilized enzyme reaction device caused by non-uniform contact between immobilized enzyme and reaction liquid and no temperature control in the prior art are solved.

Description

Technical Field

[0001] The utility model relates to the technical field of enzyme reactions, in particular to an immobilized enzyme reaction system. Background Art

[0002] Immobilized enzymes are a new technology developed in the 1960s. Immobilized enzyme technology is a key method for the industrial and laboratory application of biocatalysts. Due to their high stability, reusability, and reaction selectivity, immobilized enzymes are widely used in pharmaceuticals, food processing, environmental protection, and biochemical engineering. Compared to free enzymes, immobilized enzymes can operate under a wider range of conditions, thereby improving their economic viability and feasibility in industrial applications.

[0003] The catalytic activity of enzymes is typically very sensitive to temperature. Within a certain range, increasing temperature accelerates the enzyme's reaction rate and improves reaction efficiency. However, when the temperature exceeds the enzyme's optimal operating temperature, the enzyme's activity can be significantly reduced, or even inactivated. Furthermore, uneven contact between the reaction solution and the immobilized enzyme can lead to incomplete reaction in some reaction areas, thus affecting the overall reaction efficiency.

[0004] Chinese patent CN213085995U discloses an immobilized enzyme reaction device, which is suitable for production and application in the field of enzyme reaction technology. The immobilized enzyme reaction device includes a reaction tank, an inner wall of the reaction tank is covered with a buffer layer, and two groups of symmetrically arranged clamping sleeves are provided on the left and right sides of the reaction tank. The beneficial effects of this utility model are: when the reaction liquid is introduced into the liquid guide pipe through the liquid inlet pipe, it can overflow the entire pipe body through the opening, and then the stirring shaft and the pipe body are driven to rotate by the stirring motor, the reaction liquid can evenly seep out from the small holes and evenly react with the immobilized enzyme, and the reaction efficiency is relatively fast; when the reaction chamber shakes during the stirring process, the buffer rod applies force to the reset spring, and the buffer force when the reset spring rebounds will act on the buffer layer and offset it, which can reduce the vibration and wear of the reaction chamber during shaking, and has little effect on the overall stability of the reaction tank, thereby solving the problems of slow reaction efficiency and poor stability of the existing immobilized enzyme reaction device.

[0005] The reaction liquid in the above patent seeps out through the tube body to react with the immobilized enzyme and is stirred by a stirring paddle, so that the immobilized enzyme and the reaction liquid are in uniform contact. However, no temperature control is performed during this process, so the reaction efficiency is slow and the stability is poor. Utility Model Content

[0006] In response to the shortcomings of the existing technology, the purpose of the present invention is to propose an immobilized enzyme reaction system. Through the arrangement of a material mixing tank, a crystallization tank and a triple hollow fiber column reactor, the problem of uneven contact between the immobilized enzyme and the reaction liquid and the lack of temperature control in the existing technology, which leads to slow reaction efficiency and poor stability of the immobilized enzyme reaction device, is solved.

[0007] In order to achieve the above purpose, the present invention provides the following technical solutions:

[0008] An immobilized enzyme reaction system includes a material mixing tank, the material mixing tank is connected to a first delivery pipe, the outlet of the first delivery pipe is connected to a triple hollow fiber column reactor, the outlet of the triple hollow fiber column reactor is connected to a second delivery pipe, and the outlet of the second delivery pipe is connected to a crystallization tank;

[0009] The triple hollow fiber column reactor includes a first hollow fiber column reactor, a second hollow fiber column reactor and a third hollow fiber column reactor. The first hollow fiber column reactor, the second hollow fiber column reactor and the third hollow fiber column reactor are connected in series through a conduit; the outlet of the first delivery pipe is connected to the inlet of the first hollow fiber column reactor, and the inlet of the second delivery pipe is connected to the outlet of the third hollow fiber column reactor.

[0010] Preferably, the outer walls of the first hollow fiber column reactor, the second hollow fiber column reactor and the third hollow fiber column reactor are all provided with a third electric heating temperature control jacket, and the interiors of the first hollow fiber column reactor, the second hollow fiber column reactor and the third hollow fiber column reactor are each provided with a plurality of hollow fiber columns, and the plurality of hollow fiber columns are evenly distributed, and immobilized enzyme resins are fixed on the plurality of hollow fiber columns.

[0011] Preferably, a first feed port is provided on the top of the material mixing tank, a first discharge port is provided on the bottom, and the inlet of the first conveying pipe is connected to the first discharge port; a first motor is fixed on the top of the material mixing tank, and the output shaft of the first motor extends into the material mixing tank and is fixedly connected to a first stirring shaft, and a plurality of first stirring blades are evenly distributed and fixed on the first stirring shaft; a first electric heating temperature control jacket is provided on the outer wall of the material mixing tank.

[0012] Preferably, a second feed port is provided on the side wall of the crystallization tank, and the second feed port is connected to the outlet of the second conveying pipe; a second discharge port is provided at the bottom of the crystallization tank; a second motor is fixed on the top of the crystallization tank, and the output shaft of the second motor extends into the crystallization tank and is fixedly connected to a second stirring shaft, and a plurality of second stirring blades are evenly distributed and fixed on the second stirring shaft; a second electric heating temperature control jacket is provided on the outer wall of the crystallization tank.

[0013] Preferably, a first solution pump is installed on the first delivery pipe; and a second solution pump is installed on the second delivery pipe.

[0014] Preferably, the bottoms of the crystallization tank and the material mixing tank are both provided with supporting feet.

[0015] Preferably, a valve is installed on the second discharge port.

[0016] Compared with the prior art, the present invention has the following beneficial effects:

[0017] (1) The reaction system of the present invention mixes the substrate evenly through a material mixing tank, and after preheating to a suitable reaction temperature, starts the first solution pump to transport it to the triple hollow fiber column reactor to react with the evenly distributed immobilized enzyme. The preheating of the material mixing tank ensures that the temperature of the reaction liquid is suitable after entering the triple hollow fiber column reactor, and it can react quickly with the immobilized enzyme, thereby improving the reaction efficiency. In addition, the evenly distributed multiple hollow fiber columns can ensure that the reaction liquid and the immobilized enzyme achieve a uniform reaction. Furthermore, by controlling the flow rates of the first solution pump and the second solution pump, the reaction rate and reaction progress are adjusted, the shaking of the triple hollow fiber column reactor is reduced, the overall stability of the immobilized enzyme on the hollow fiber column is improved, and the problems of slow reaction efficiency and poor stability of the existing immobilized enzyme reaction device are solved.

[0018] (2) The reaction system of the utility model is provided with electric heating temperature control jackets on the material mixing tank, the triple hollow fiber column reactor and the crystallization tank, respectively, which ensures the reaction temperature during the operation of the device, so that the reaction can be maintained at a high efficiency, and the device is highly practical. BRIEF DESCRIPTION OF THE DRAWINGS

[0019] Figure 1 This is a schematic diagram of the overall structure of an immobilized enzyme reaction system of the utility model;

[0020] Figure 2 The utility model is a schematic diagram of the cross-sectional structure of a hollow fiber column reactor of an immobilized enzyme reaction system.

[0021] In the figure: wherein: 100, material mixing tank; 200, first conveying pipe; 300, triple hollow fiber column reactor; 400, second conveying pipe; 500, crystallization tank; 110, first feed port; 120, first discharge port; 130, first motor; 140, first stirring shaft; 150, first stirring blade; 160, first electric temperature control jacket; 210, first solution pump; 310, first hollow fiber column reactor; 320, second hollow fiber column reactor; 330, third hollow fiber column reactor; 340, third electric temperature control jacket; 350, hollow fiber column; 360, conduit; 410, second solution pump; 510, second feed port; 520, second discharge port; 530, second motor; 540, second stirring shaft; 550, second stirring blade; 560, second electric temperature control jacket. DETAILED DESCRIPTION

[0022] The following will be combined with the accompanying drawings in the embodiments of the present invention to clearly and completely describe the technical solutions in the embodiments of the present invention. Obviously, the embodiments described are part of the embodiments of the present invention, not all of the embodiments. Based on the embodiments of the present invention, all other embodiments obtained by ordinary technicians in this field without making creative efforts are within the scope of protection of the present invention.

[0023] Example

[0024] like Figures 1 and 2 As shown, an immobilized enzyme reaction system includes a material mixing tank 100, the material mixing tank 100 is connected to a first delivery pipe 200, the outlet of the first delivery pipe 200 is connected to a triple hollow fiber column reactor 300, the outlet of the triple hollow fiber column reactor 300 is connected to a second delivery pipe 400, and the outlet of the second delivery pipe 400 is connected to a crystallization tank 500;

[0025] The reaction substrate enters the material mixing tank 100, is mixed in the material mixing tank 100, and is transported to the triple hollow fiber column reactor 300 through the first delivery pipe 200 to react with the immobilized enzyme. After the reaction is completed, it is transported to the crystallization tank 500 through the second delivery pipe 400 for crystallization. After the crystallization is completed, it is discharged and collected;

[0026] The triple hollow fiber column reactor 300 includes a first hollow fiber column reactor 310, a second hollow fiber column reactor 320 and a third hollow fiber column reactor 330. The first hollow fiber column reactor 310, the second hollow fiber column reactor 320 and the third hollow fiber column reactor 330 are connected in series through a conduit 360; the outlet of the first conveying pipe 200 is connected to the inlet of the first hollow fiber column reactor 310, and the inlet of the second conveying pipe 400 is connected to the outlet of the third hollow fiber column reactor 330.

[0027] The reaction substrate is transported to the triple hollow fiber column reactor 300 by the first delivery pipe 200 and reacts step by step. It first enters the first hollow fiber column reactor 310. The reaction of the first hollow fiber column reactor 310 reaches about 60%. It is continuously transported through the first delivery pipe 200 and gradually enters the second hollow fiber column reactor 320. The second hollow fiber column reactor 320 completes 90% of the reaction and finally enters the third hollow fiber column reactor 330 to achieve a complete reaction, that is, the effect of a complete reaction is achieved, and the reaction effect is good.

[0028] In this embodiment, the outer walls of the first hollow fiber column reactor 310, the second hollow fiber column reactor 320 and the third hollow fiber column reactor 330 are all provided with a third electric heating temperature control jacket 340, and the interiors of the first hollow fiber column reactor 310, the second hollow fiber column reactor 320 and the third hollow fiber column reactor 330 are all provided with multiple hollow fiber columns 350, and the multiple hollow fiber columns 350 are evenly distributed, and immobilized enzyme resins are fixed on the multiple hollow fiber columns 350.

[0029] Each hollow fiber column reactor is provided with a third electric heating temperature control jacket 340 on the outside. When the reaction substrate enters the hollow fiber column reactor, the reaction substrate reacts with a plurality of evenly distributed hollow fiber columns 350, making the reaction more uniform. Furthermore, the heating of the third electric heating temperature control jacket 340 can optimize the reaction temperature, so that the device can achieve excellent reaction effect.

[0030] In this embodiment, the material mixing tank 100 is provided with a first feed port 110 on the top and a first discharge port 120 on the bottom, and the inlet of the first conveying pipe 200 is connected to the first discharge port 120; a first motor 130 is fixed on the top of the material mixing tank 100, and the output shaft of the first motor 130 extends into the material mixing tank 100 and is fixedly connected to a first stirring shaft 140, and a plurality of first stirring blades 150 are evenly distributed and fixed on the first stirring shaft 140; a first electric heating temperature control jacket 160 is provided on the outer wall of the material mixing tank 100.

[0031] The substrate enters the material mixing tank 100 from the first feed port 110, and the first stirring shaft 140 is driven to rotate by the first motor 130, and the stirring blade 150 is driven to stir evenly, so that the substrate is mixed evenly. During mixing, the reaction substrate is heated by the first electric heating temperature control jacket 160, so that when the substrate after stirring enters the triple hollow fiber column reactor 300, it can quickly reach the optimal reaction state with the immobilized enzyme, and the reaction effect is good.

[0032] In this embodiment, a second feed port 510 is provided on the side wall of the crystallization tank 500, and the second feed port 510 is connected to the outlet of the second conveying pipe 400; a second discharge port 520 is provided at the bottom of the crystallization tank 500; a second motor 530 is fixed to the top of the crystallization tank 500, and the output shaft of the second motor 530 extends into the crystallization tank 500 and is fixedly connected to a second stirring shaft 540, and a plurality of second stirring blades 550 are evenly distributed and fixed on the second stirring shaft 540; a second electric heating temperature control jacket 560 is provided on the outer wall of the crystallization tank 500.

[0033] After the reaction is completed, the substrate enters the crystallization tank 500 and needs to be concentrated and dried. The second motor 530 drives the second stirring shaft 540 to rotate, so that the second stirring blade 550 stirs the bottom of the reaction. The second electric heating temperature control jacket 560 can heat the device so that it can be quickly concentrated and dried to obtain the product.

[0034] In this embodiment, a first solution pump 210 is installed on the first delivery pipe 200 ; and a second solution pump 410 is installed on the second delivery pipe 400 .

[0035] The first solution pump 210 and the second solution pump 410 provide power for the delivery of the substrate. By informing the flow rate of the pump, the reaction rate can be adjusted and the stability of the immobilized enzyme can be increased.

[0036] In this embodiment, the bottoms of the crystallization tank 500 and the material mixing tank 100 are both provided with supporting feet.

[0037] The supporting legs support the crystallization tank 500 and the material mixing tank 100 . At the same time, the arrangement of the supporting legs enables the discharge of materials from the discharge ports of the crystallization tank 500 and the material mixing tank 100 to be smoother.

[0038] In this embodiment, a valve is installed on the second discharge port 520 .

[0039] The working principle of the immobilized enzyme reaction system of the utility model is as follows:

[0040] The substrate enters the material mixing tank 100 from the first feed port 110, and the first stirring shaft 140 is driven to rotate by the first motor 130, and the stirring blade 150 is driven to stir evenly, so that the substrate is mixed evenly. During the mixing, the reaction substrate is heated by the first electric heating temperature control jacket 160. After the mixing is completed, the first solution pump 210 is turned on, and the reaction substrate is transported to the triple hollow fiber column reactor 300 by the first delivery pipe 200 for step-by-step reaction. First, it enters the first hollow fiber column reactor 310, and the reaction of the first hollow fiber column reactor 310 reaches 6 0%, and is continuously transported through the first conveying pipe 200, gradually entering the second hollow fiber column reactor 320, the second hollow fiber column reactor 320 completes 90% of the reaction, and finally enters the third hollow fiber column reactor 330 to achieve complete reaction; after the reaction is completed, the substrate enters the crystallization tank 500, and the second motor 530 drives the second stirring shaft 540 to rotate, so that the second stirring blade 550 stirs the bottom where the reaction is completed, and the device can be heated by the second electric heating temperature control jacket 560, so that it can be quickly concentrated and dried, and the obtained product is discharged from the second discharge port 520.

[0041] The above is only a preferred specific implementation method of the present invention, but the protection scope of the present invention is not limited to this. Any technician familiar with the technical field within the technical scope disclosed by the present invention can make equivalent replacements or changes based on the technical solution and utility model concept of the present invention, which should be covered by the protection scope of the present invention.

Claims

1. An immobilized enzyme reaction system, comprising a material mixing tank (100), characterized in that: The material mixing tank (100) is connected to a first delivery pipe (200), the outlet of the first delivery pipe (200) is connected to a triple hollow fiber column reactor (300), the outlet of the triple hollow fiber column reactor (300) is connected to a second delivery pipe (400), and the outlet of the second delivery pipe (400) is connected to a crystallization tank (500); The triple hollow fiber column reactor (300) includes a first hollow fiber column reactor (310), a second hollow fiber column reactor (320) and a third hollow fiber column reactor (330), and the first hollow fiber column reactor (310), the second hollow fiber column reactor (320) and the third hollow fiber column reactor (330) are connected in series through a conduit (360); the outlet of the first conveying pipe (200) is connected to the inlet of the first hollow fiber column reactor (310), and the inlet of the second conveying pipe (400) is connected to the outlet of the third hollow fiber column reactor (330).

2. The immobilized enzyme reaction system according to claim 1, characterized in that: The outer walls of the first hollow fiber column reactor (310), the second hollow fiber column reactor (320) and the third hollow fiber column reactor (330) are all provided with a third electric heating temperature control jacket (340), and the interiors of the first hollow fiber column reactor (310), the second hollow fiber column reactor (320) and the third hollow fiber column reactor (330) are all provided with a plurality of hollow fiber columns (350), and the plurality of hollow fiber columns (350) are evenly distributed, and immobilized enzyme resin is fixed on the plurality of hollow fiber columns (350).

3. The immobilized enzyme reaction system according to claim 1, characterized in that: The material mixing tank (100) is provided with a first feed port (110) at the top and a first discharge port (120) at the bottom, and the inlet of the first conveying pipe (200) is communicated with the first discharge port (120); a first motor (130) is fixed on the top of the material mixing tank (100), and the output shaft of the first motor (130) extends into the material mixing tank (100) and is fixedly connected to a first stirring shaft (140), and a plurality of first stirring blades (150) are evenly distributed and fixed on the first stirring shaft (140); and a first electric heating temperature control jacket (160) is sleeved on the outer wall of the material mixing tank (100).

4. The immobilized enzyme reaction system according to claim 1, characterized in that: A second feed port (510) is provided on the side wall of the crystallizer (500), and the second feed port (510) is communicated with the outlet of the second conveying pipe (400); a second discharge port (520) is provided at the bottom of the crystallizer (500); a second motor (530) is fixed on the top of the crystallizer (500), and the output shaft of the second motor (530) extends into the crystallizer (500) and is fixedly connected to a second stirring shaft (540), and a plurality of second stirring blades (550) are evenly distributed and fixed on the second stirring shaft (540); a second electric heating temperature control jacket (560) is provided on the outer wall of the crystallizer (500).

5. The immobilized enzyme reaction system according to claim 1, characterized in that: A first solution pump (210) is installed on the first delivery pipe (200); and a second solution pump (410) is installed on the second delivery pipe (400).

6. The immobilized enzyme reaction system according to claim 1, characterized in that: The bottoms of the crystallization tank (500) and the material mixing tank (100) are both provided with supporting feet.

7. The immobilized enzyme reaction system according to claim 4, characterized in that: A valve is installed on the second discharge port (520).

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