Support for storing and taking confocal culture dishes

Abstract

The utility model discloses a support for storing and taking confocal culture dishes, which belongs to the technical field of experimental tools, and comprises a bottom plate, a first groove is arranged in the bottom plate, a plurality of first placing grooves are arranged in the first groove at equal intervals, a top cover is arranged on the top surface of the bottom plate, the top cover is matched with the bottom plate, a second groove is arranged in the top cover, and a plurality of second placing grooves are arranged in the second groove. A plurality of first containing grooves are formed in the first groove at equal intervals, a plurality of second containing grooves are formed in the second groove at equal intervals, the first containing grooves correspond to the second containing grooves in position, first fences are symmetrically arranged on the two sides of each first containing groove and located in the first groove, and second fences are symmetrically arranged on the two sides of each second containing groove. The bracket of the confocal culture dish is simpler and more convenient in the experiment use process, the direct operation of integration of the bracket and the confocal culture dish in the experiment process is basically realized, the bracket and the confocal culture dish can be randomly taken in necessary periods, and the free conversion of integration and separation is embodied. When the bracket is used for the confocal culture dish, the advantage of convenience in taking is reserved, and meanwhile, the taking frequency is reduced.

Description

Technical Field

[0001] The utility model belongs to the technical field of experimental tools, in particular to a bracket for storing and taking out a confocal culture dish. Background Art

[0002] Confocal microscopy dishes are individual, 35mm round dishes consisting of a base and lid. They are primarily used for cell culture observation under confocal microscopy, particularly for experiments involving live-cell imaging, and are therefore irreplaceable laboratory supplies. However, handling and storage of confocal dishes during experiments present numerous drawbacks, hindering the overall experimental process and, in severe cases, even leading to experimental failure. The following are the main drawbacks to handling and storage of confocal dishes during experiments: 1. They are difficult to handle and stack. When stacking more than three dishes, stacking them together can easily cause them to slip. 2. They are difficult to transport, especially for stacking confocal dishes in a cell culture incubator and subsequently transferring them to a cell microscope for observation of cell growth, or for live-cell experiments under a confocal microscope. 3. They are difficult to label. The 35mm size of the dish limits labeling space, requiring both the base and lid to avoid confusion during experiments, especially during cell culture changes or live-cell experiments. 4. They are difficult to handle for experimental procedures such as cell culture changes and washing. Because they are numerous, they cannot be stacked, and scattered individually on the workbench, creating a cluttered and inconvenient environment. 5. It is not conducive to storage. For some samples that have undergone immunofluorescence or immunohistochemistry experiments, confocal dishes are easy to scatter due to stacking, which is not conducive to storage. 6. It is not conducive to light protection. Some experimental processes require light protection. Confocal culture dishes are round and easy to slide, which is not conducive to light protection with tin foil or other methods. 7. It is not conducive to direct and rapid observation of the growth status of cells. Individual cells need to be observed under a cell microscope. There is no suitable slide card slot, and the culture dish can only be moved manually to observe cells. Especially when it comes to fluorescence photography, it is even more difficult to operate in a dark room. Therefore, there is an urgent need for a confocal culture dish storage and retrieval bracket to solve the above problems. Utility Model Content

[0003] The purpose of the utility model is to provide a support for storing and taking out a confocal culture dish, so as to solve the problems existing in the above-mentioned prior art.

[0004] To achieve the above-mentioned purpose, the present invention provides the following solution: The present invention provides a bracket for storing and taking out confocal culture dishes, comprising a base plate, a first groove is provided in the base plate, a plurality of first placement grooves are provided at equal intervals in the first groove, a top cover is provided on the top surface of the base plate, the top cover is adapted to the base plate, a second groove is provided in the top cover, a plurality of second placement grooves are provided at equal intervals in the second groove, the first placement groove and the second placement groove are arranged in corresponding positions, first fences are symmetrically provided on both sides of the first placement groove, the first fence is located in the first groove, second fences are symmetrically provided on both sides of the second placement groove, the second fence is located in the second groove.

[0005] Preferably, a third fence is provided in the base plate, and the surface of the third fence is higher than the edge of the base plate.

[0006] Preferably, first through holes are symmetrically provided in the bottom plate and the top cover respectively.

[0007] Preferably, the bottom radius of the first placement groove is 12.5 mm, the top radius is 17 mm, and the vertical height is 3 mm.

[0008] Preferably, both sides of the first fence are provided with arcs with a radius of 17.7 mm, and the height of the first fence is 1.5 mm.

[0009] Preferably, the height of the top cover is 14 mm, and the height of the second fence is 2 mm.

[0010] The utility model discloses the following technical effects: The holder for the confocal culture dish of the utility model is more convenient to use during experiments, essentially realizing direct operation of the holder and the confocal culture dish during the experiment, and can be freely taken out when necessary, embodying the free conversion between integration and separation. The use of the confocal culture dish holder of the utility model not only retains the advantage of convenient taking out, but also reduces the number of times to take out. If a long operation is required, the dish cover can be placed in the dish cover placement slot of the corresponding lid, thereby reducing experimental operation errors and reducing cross contamination. BRIEF DESCRIPTION OF THE DRAWINGS

[0011] The accompanying drawings, which constitute part of this application, are intended to provide a further understanding of this application. The exemplary embodiments and descriptions of this application are intended to explain this application and do not constitute an improper limitation on this application. In the accompanying drawings:

[0012] Figure 1 This is a schematic diagram of the structure of the support for storing and taking out the confocal culture dish of the present invention.

[0013] In the figure: 1. bottom plate; 2. first groove; 3. first placement groove; 4. top cover; 5. second groove; 6. second placement groove; 7. first fence; 8. second fence; 9. third fence; 10. first through hole; 11. marking opening. DETAILED DESCRIPTION

[0014] The following will be combined with the drawings in the embodiments of the present invention to clearly and completely describe the technical solutions in the embodiments of the present invention. Obviously, the embodiments described are only part of the embodiments of the present invention, not all of the embodiments. Based on the embodiments of the present invention, all other embodiments obtained by ordinary technicians in this field without making creative efforts are within the scope of protection of the present invention.

[0015] In order to make the above-mentioned objects, features and advantages of the present invention more obvious and easy to understand, the present invention is further described in detail below with reference to the accompanying drawings and specific implementation methods.

[0016] A bracket for storing and taking out confocal culture dishes includes a base plate 1, a first groove 2 is provided in the base plate 1, and a plurality of first placement grooves 3 are evenly spaced in the first groove 2. A top cover 4 is provided on the top surface of the base plate 1, and the top cover 4 is adapted to the base plate 1. A second groove 5 is provided in the top cover 4, and a plurality of second placement grooves 6 are evenly spaced in the second groove 5. The first placement groove 3 and the second placement groove 6 are arranged in corresponding positions. First fences 7 are symmetrically provided on both sides of the first placement groove 3, and the first fence 7 is located in the first groove 2. Second fences 8 are symmetrically provided on both sides of the second placement groove 6, and the second fences 8 are located in the second groove 5.

[0017] This application is a single-layer structure, and each layer can hold 6, which is a large number. Three repeated experiments can be performed in the same space. The single-layer structure is convenient for liquid replacement and operation. By setting multiple first placement slots 3 and second anti-placement slots, confocal culture dishes can be stacked and stored at will. The entire support together with the culture dish can be placed under a cell microscope, moved and observed at will, and is suitable for live cell fluorescence photography in a dark room. For experiments involving light protection, tin foil can be used to protect a single culture dish or the entire support of 6 culture dishes from light, which is simple to protect from light.

[0018] The total height of the bottom plate 1 and the top cover 4 is 21 mm, and the total height of the bottom plate 1 is 7 mm, which is used to support the top cover 4 and prevent the top cover 4 from sliding.

[0019] This new confocal culture dish holder simplifies experimental use, essentially integrating the holder and the confocal culture dish for direct operation during the experiment. It can be removed at will when necessary, embodying the flexible transition between integration and separation. Using this new holder for confocal culture dishes not only maintains the advantage of easy access, but also reduces the number of times they need to be removed. For extended operations, the dish lid can be placed in the corresponding lid slot, minimizing experimental errors and cross-contamination.

[0020] It has a holder similar to a 6-well plate, which can be used to mark and classify at multiple locations on the shelf; one holder can hold 6 culture dishes, and multiple holders can be used for large-scale use, which can be stacked in the cell culture incubator without taking up space and are easy to take; when light protection is needed, it can be achieved by directly wrapping it with tinfoil; when samples need to be stored after experiments such as immunofluorescence, they can still be stacked after being wrapped with tinfoil.

[0021] The confocal culture dishes can be placed evenly and firmly in the placement slots, avoiding the disadvantage of unstable stacking of confocal culture dishes and the need to lay them flat, reducing the space used in the cell culture incubator, and reducing the problem of individual confocal culture dishes being easily overturned when stacked, avoiding problems such as knocking over during the experiment that may lead to experimental failure.

[0022] The bracket in this application is modified on a standard 6-well plate, which reduces the production and debugging of molds in the production process, saves processes, and improves production efficiency; in the use of raw materials, a hollow structure is adopted compared to the standard 6-well plate, which reduces the height of the bottom and the material usage of the entire plate; because it is a modification of a 6-well plate, the production process can be consistent with the mature 6-well plate, and compared with the existing technology, its production quality and precision are improved.

[0023] The bracket material of this application can refer to the raw materials of the 6-well plate, and can use a variety of materials such as plastic, without the need to use materials such as metal or stainless steel, reducing the consumables of raw materials, thereby reducing energy consumption in the entire industry; this bracket can use recycled plastic or other energy materials for replacement processing, because the hollow structure is retained, there is no need to consider the transparency of the bracket, reducing environmental pollution.

[0024] A further optimization scheme is to install a third fence 9 inside the bottom plate 1. The surface of the third fence 9 is higher than the edge of the bottom plate 1. The edge of the third fence 9 is uniformly 1mm smaller than that of the bottom plate 1 and is used to support the top cover 4. The width of the third fence 9 is 1mm. The height of the first groove 2 is 3mm. The height of the third fence 9 is 11mm higher than the bottom plate.

[0025] According to a further optimized solution, first through holes 10 are symmetrically provided in the bottom plate 1 and the top cover 4. The radius of the first through hole 10 is 5 mm.

[0026] According to a further optimized solution, the first placement groove 3 has a bottom radius of 12.5 mm, an upper radius of 17 mm, and a vertical height of 3 mm, and is used for placing the confocal culture dish.

[0027] A further optimization scheme was developed, with arcs of 17.7mm radius on either side of the first fence 7, and a height of 1.5mm. This arrangement effectively limits the shaking of the culture dish, preventing movement during experimental operation and movement; it also provides more space for quick access to the confocal culture dish.

[0028] Further optimization scheme, the height of top cover 4 is 14mm, the height of second fence 8 is 2mm. The top edge of top cover 4 is indented by 1mm, the height is 3mm, and the width is 1mm, which is helpful for stacking with other brackets.

[0029] A marking opening 11 is provided on the bottom plate 1 .

[0030] Base plate 1 is the same size and height as the outer base plate of a standard 6-well plate, supporting the frame and fitting within the observation platform of optical and fluorescence microscopes. Whether the confocal culture dish is placed within the frame or on base plate 1, it does not affect observation of the dish in the optical culture chamber. During the cell culture stage, samples on the frame are more convenient to observe, eliminating the need to repeatedly open and close the cell culture incubator. Cells with different treatments can be placed on the same frame for easy photography and comparative observation.

[0031] Confocal culture dishes are mainly used in cell experiments such as live cell imaging and high-content imaging analysis. The cell culture involves multiple steps such as cell fluid replacement, fluid replacement and cleaning during biochemical experiments. The lid is provided with a placement groove similar to the bottom plate 1. Its design is consistent with the placement groove of the bottom plate 1, and the circular size of the placement groove is consistent. If a few experiments require long-term operation, the corresponding culture dish lid can be placed in the corresponding position to avoid accidental picking and cross-contamination.

[0032] Samples in confocal culture dishes require protection from light throughout the entire experiment, including immunofluorescence and fluorescence conversion experiments. This protection must be quick and easy to perform, allowing for fluid or antibody changes. This holder effectively solves this problem. The confocal dish can be quickly wrapped in tinfoil to protect it from light. For fluid or antibody changes, simply remove the foil and proceed quickly. When finished, simply wrap the dish back in tinfoil. No additional cover is required.

[0033] In the description of the present invention, it should be understood that the terms "longitudinal", "transverse", "up", "down", "front", "back", "left", "right", "vertical", "horizontal", "top", "bottom", "inside", "outside", etc., indicating the orientation or position relationship, are based on the orientation or position relationship shown in the accompanying drawings, and are only for the convenience of describing the present invention, and do not indicate or imply that the device or element referred to must have a specific orientation, be constructed and operated in a specific orientation. Therefore, they should not be understood as limitations on the present invention.

[0034] The embodiments described above are merely preferred embodiments of the present invention and are not intended to limit the scope of the present invention. Without departing from the spirit of the present invention, various modifications and improvements to the technical solutions of the present invention made by ordinary technicians in this field should fall within the scope of protection determined by the claims of the present invention.

Claims

1. A holder for storing and taking out confocal culture dishes, characterized by: The invention comprises a bottom plate (1), wherein a first groove (2) is provided in the bottom plate (1), and a plurality of first placement grooves (3) are provided in the first groove (2) at equal intervals, and a top cover (4) is provided on the top surface of the bottom plate (1), and the top cover (4) is adapted to the bottom plate (1), and a second groove (5) is provided in the top cover (4), and a plurality of second placement grooves (6) are provided in the second groove (5) at equal intervals, and the first placement groove (3) and the second placement groove (6) are arranged in corresponding positions, and first fences (7) are symmetrically provided on both sides of the first placement groove (3), and the first fences (7) are located in the first groove (2), and second fences (8) are symmetrically provided on both sides of the second placement groove (6), and the second fences (8) are located in the second groove (5).

2. The confocal culture dish storage and retrieval support according to claim 1, characterized in that: A third fence (9) is provided inside the base plate (1), and the surface of the third fence (9) is higher than the edge of the base plate (1).

3. The confocal culture dish storage and retrieval support according to claim 1, characterized in that: First through holes (10) are symmetrically provided in the bottom plate (1) and the top cover (4).

4. The confocal culture dish storage and retrieval support according to claim 1, characterized in that: The first placement groove (3) has a bottom radius of 12.5 mm, an upper radius of 17 mm, and a vertical height of 3 mm.

5. The confocal culture dish storage and retrieval support according to claim 1, characterized in that: Arcs with a radius of 17.7 mm are respectively provided on both sides of the first fence (7), and the height of the first fence (7) is 1.5 mm.

6. The confocal culture dish storage and retrieval support according to claim 1, characterized in that: The height of the top cover (4) is 14 mm, and the height of the second fence (8) is 2 mm.

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