High-flux flow-type fluorescence measurement device

Abstract

The utility model relates to the technical field of medical equipment, and discloses a high-flux flow type fluorescence determination device which comprises a main box body, a box body cover plate is clamped on the upper surface of the main box body, and a sheath liquid barrel, a water pump, a hose and a spray head are inserted on the upper surface of the box body cover plate. The sheath liquid detection device is reasonable in structure, sheath liquid is added into the sheath liquid barrel from the water inlet pipe on the upper surface of the sheath liquid cover plate by a worker, a detection sample is added into the sample hopper by the sample inlet plug on the upper surface of the sample cover plate, and the sample in the sheath liquid is detected by using different laser lamps; the problems that the detection amount of a sample is inconvenient to control each time during detection, detection is inaccurate, and grouping detection cannot be conducted for multiple times are avoided, a cleaning agent in a cleaning box is pumped into a hose through a water pump and sprayed out through a water outlet hole in the lower surface of a spray head, and the problems that cleaning is inconvenient after workers use the cleaning agent, the service life of equipment is shortened, and the working efficiency is improved are solved. The detection accuracy is reduced and the working efficiency is influenced because the equipment is not cleaned after long-time use.

Description

Technical Field

[0001] The utility model relates to the technical field of medical equipment, in particular to a high-throughput flow fluorescence measurement device. Background Art

[0002] The flow cytometer, with fluorescently encoded microspheres at its core, is a multi-index parallel analysis technology platform that integrates flow cytometry, laser analysis, high-speed digital signal processing, and other technologies. It can accurately and quantitatively detect 100 different biomolecules simultaneously. It has the characteristics of high throughput, high sensitivity, and parallel detection. It can be used for research in many aspects and fields, such as immunoassay, nucleic acid research, enzymology analysis, receptor and ligand recognition analysis.

[0003] A multifunctional fully automatic flow fluorescence detection device disclosed in the Chinese utility model patent application disclosure specification CN214703331U, although the cover plate is covered by a shielding cloth, and the sliding hole on the cover plate can be sealed by the shielding cloth when the fully automatic flow fluorescence detection device is not in use, thereby preventing dust from accumulating in the box body, and at the same time, when the hook is separated from the hanging ring, the shielding cloth can be automatically wound around the third rotating shaft through the spring spring, thereby facilitating the user to store the shielding cloth, but the existing equipment cannot perform multiple groups of separate detection on the samples to be detected, resulting in inaccurate detection and unnecessary waste, and cannot control different laser lights for detection, the detection is not comprehensive and diverse, and it is inconvenient to clean the equipment, which wastes working time and reduces the service life of the equipment. If the equipment is not cleaned after long-term use, it is easy to cause reduced detection accuracy and reduced work efficiency. Therefore, we propose a new device to solve the above problems. Utility Model Content

[0004] (1) Technical problems solved

[0005] In response to the shortcomings of the existing technology, the utility model provides a high-throughput flow fluorescence measurement device, which solves the problems of being unable to test multiple groups of samples separately, the lack of comprehensive and diverse testing, the inconvenience of cleaning the equipment, which reduces the service life of the equipment, the impact of long-term cleaning on detection accuracy, and low work efficiency.

[0006] (2) Technical solution

[0007] To achieve the above objectives, the present invention is implemented through the following technical solutions: a high-throughput flow fluorescence measurement device, comprising a main box, the upper surface of the main box is clamped with a box cover, the upper surface of the box cover is plugged with a sheath liquid barrel, the upper surface of the sheath liquid barrel is clamped with the sheath liquid cover, the lower surface of the sheath liquid barrel is plugged with a first pipe, the upper surface of the box cover is plugged with a sample hopper, the upper surface of the sample hopper is clamped with the sample cover, the upper surface of the sample cover is plugged with a sample input plug, a sample dividing plug is threaded through one side of the sample hopper, a second pipe is plugged with the lower surface of the sample hopper, a waste liquid tank is sleeved on one side of the main box, the upper surface of the waste liquid tank is clamped with the waste liquid cover, the inner side wall of the main box is provided with a moving motor, one end of the moving motor is sleeved with a first screw, the inner side wall of the main box is slidably connected to a laser lamp, the other side of the main box is sleeved with a placement ring, and the other side of the main box is sleeved with a cleaning assembly.

[0008] Optionally, the cleaning assembly includes a cleaning box, a water pump, a hose, a nozzle and a battery. The water pump is connected to the inside of the cleaning box, the upper end of the water pump is connected to the hose, one end of the hose is connected to the nozzle, and a battery is provided on the upper surface of the cleaning box.

[0009] Optionally, a plurality of water outlet holes are provided on the lower surface of the nozzle, and a water inlet pipe is provided on the upper surface of the cleaning box.

[0010] Optionally, a threaded hole is provided inside the laser lamp, and the threaded hole and the first lead screw are adapted to each other.

[0011] Optionally, a water inlet pipe is provided on the upper surface of the sheath liquid cover plate, and a pipe hole is opened on the upper surface of the box cover plate, and the pipe hole is consistent in size with the first pipe.

[0012] Optionally, a hole is provided on one side of the second pipe, the hole is connected to the first pipe, and a pipe hole is provided on the upper surface of the box cover, and the pipe hole is the same size as the second pipe.

[0013] Optionally, a pipe hole is provided on one side of the main box body, a hole is provided on the other side of the waste liquid tank, and the second pipe passes through the pipe hole and penetrates the waste liquid tank.

[0014] Optionally, a bolt is threaded through the upper surface of the box cover, and the box cover is threaded through the main box body via the bolt thread.

[0015] In summary, the technical effects and advantages of the utility model are:

[0016] 1. The utility model has a reasonable structure. A staff member adds sheath liquid into the sheath liquid barrel from the water inlet pipe on the upper surface of the sheath liquid cover plate, adds the test sample into the sample hopper through the sample inlet plug on the upper surface of the sample cover plate, and controls the test amount of the sample by rotating the sample dividing plug. The sheath liquid in the sheath liquid barrel flows into the second pipe through the first pipe, and the sample and sheath liquid are mixed through the second pipe. The first lead screw is rotated by the moving motor to move the laser lamp to different positions, and different laser lamps are used to test the sample in the sheath liquid. The tested sample and sheath liquid flow into the waste liquid tank, avoiding the inconvenience of controlling the test amount of each sample during the test, resulting in inaccurate test and unnecessary waste, and the inconvenience of multiple group tests, the inconvenience of moving the laser lamp, the inability to perform different laser lamp tests, the inconvenience of test efficiency, and the control of the test amount by the sample dividing plug. Multiple and multiple group tests can be performed, the test is more accurate, and the waste of resources is avoided. The use of different laser lamps for testing can achieve more comprehensive and diverse results, thereby improving the test efficiency.

[0017] The cleaning agent is poured into the cleaning tank through the water inlet pipe on the upper surface of the cleaning box, and the cleaning agent in the cleaning tank is pumped into the hose by the water pump and sprayed out through the water outlet hole on the lower surface of the nozzle. The worker holds the nozzle and sprays the cleaning agent to the sheath liquid barrel for cleaning. The cleaning agent flows from the first pipe into the second pipe, and the nozzle is sprayed into the sample hopper for cleaning. The cleaning agent flows from the second pipe into the waste liquid tank. The waste liquid tank is taken out, the waste liquid cover is opened and the cleaning agent after cleaning is poured out. The worker can also clean the surface of the equipment by holding the nozzle. It is avoided that the worker is inconvenient to clean after use, wastes working time, and shortens the service life of the equipment. If the equipment is not cleaned after long-term use, it is easy to cause the detection accuracy to decrease and affect the work efficiency. Therefore, it is convenient for the worker to use the equipment through the cleaning component, improves the cleaning speed, keeps the equipment clean, does not deviate when the worker is performing detection, prolongs the service life of the equipment, reduces working cost, and improves work efficiency. BRIEF DESCRIPTION OF THE DRAWINGS

[0018] Figure 1 This is a schematic diagram of the structure of the utility model;

[0019] Figure 2 This is an exploded schematic diagram of the main box structure of the utility model;

[0020] Figure 3 This is an exploded schematic diagram of the box cover structure of the utility model;

[0021] Figure 4 This is a schematic diagram of the explosion of the laser lamp structure of the utility model;

[0022] Figure 5This is a schematic diagram of the structure of the cleaning component of the utility model.

[0023] In the figure: 1. Main box; 2. Box cover; 3. Sheath fluid barrel; 4. Sheath fluid cover; 5. First pipeline; 6. Sample hopper; 7. Sample cover; 8. Sample inlet plug; 9. Sample dividing plug; 10. Second pipeline; 11. Waste liquid tank; 12. Waste liquid cover; 13. Moving motor; 14. First screw; 15. Laser light; 16. Placement ring; 17. Cleaning assembly; 1701. Cleaning box; 1702. Water pump; 1703. Hose; 1704. Nozzle; 1705. Battery. DETAILED DESCRIPTION

[0024] The following will be combined with the drawings in the embodiments of the present invention to clearly and completely describe the technical solutions in the embodiments of the present invention. Obviously, the embodiments described are only part of the embodiments of the present invention, not all of the embodiments. Based on the embodiments of the present invention, all other embodiments obtained by ordinary technicians in this field without making creative efforts are within the scope of protection of the present invention.

[0025] Example: Reference Figure 1-Figure 5 The high-throughput flow fluorescence assay device shown in the figure comprises a main box 1, a box cover 2 is clamped on the upper surface of the main box 1, a sheath liquid barrel 3 is plugged into the upper surface of the box cover 2, a sheath liquid cover 4 is clamped on the upper surface of the sheath liquid barrel 3, a first pipe 5 is plugged into the lower surface of the sheath liquid barrel 3, a sample hopper 6 is plugged into the upper surface of the box cover 2, a sample cover 7 is clamped on the upper surface of the sample hopper 6, a sample plug 8 is plugged into the upper surface of the sample cover 7, and a threaded hole on one side of the sample hopper 6 is provided. A sample dividing plug 9 is passed through, a second pipe 10 is connected to the lower surface of the sample hopper 6, a waste liquid tank 11 is sleeved on one side of the main box body 1, a waste liquid cover plate 12 is clamped on the upper surface of the waste liquid tank 11, a moving motor 13 is provided on the inner wall of the main box body 1, a first lead screw 14 is sleeved on one end of the moving motor 13, a laser lamp 15 is slidably connected to the inner wall of the main box body 1, a placement ring 16 is inserted on the other side of the main box body 1, and a cleaning component 17 is sleeved on the other side of the main box body 1.

[0026] As a preferred implementation in this embodiment, Figures 2 to 4As shown, the upper surface of the main box body 1 is clamped with a box cover 2, and the upper surface of the box cover 2 is threaded with a bolt, and the box cover 2 is threaded with the main box body 1 through the bolt thread. A pipe hole is provided on one side of the main box body 1, and a sheath liquid barrel 3 is plugged into the upper surface of the box cover 2, and the upper surface of the sheath liquid barrel 3 is clamped with a sheath liquid cover 4. The upper surface of the sheath liquid cover 4 is provided with a water inlet pipe, and the lower surface of the sheath liquid barrel 3 is plugged with a first pipe 5. A pipe hole is provided on the upper surface of the box cover 2, and the pipe hole is consistent in size with the first pipe 5. A sample hopper 6 is plugged into the upper surface of the box cover 2, and the upper surface of the sample hopper 6 is clamped with a sample cover 7, and the upper surface of the sample cover 7 is plugged There is a sample plug 8, a sample plug 9 is threaded through one side of the sample hopper 6, a second pipe 10 is plugged into the lower surface of the sample hopper 6, a hole is provided on one side of the second pipe 10, and the hole is connected to the first pipe 5. A pipe hole is provided on the upper surface of the box cover 2, and the pipe hole is the same size as the second pipe 10. A waste liquid tank 11 is sleeved on one side of the main box 1, and a hole is provided on the other side of the waste liquid tank 11. The second pipe 10 passes through the pipe hole in the waste liquid tank 11, and a waste liquid cover 12 is clamped on the upper surface of the waste liquid tank 11. A moving motor 13 is provided on the inner side wall of the main box 1, and a first screw 14 is sleeved on one end of the moving motor 13. The inner wall of the main box 1 The side wall is slidably connected to a laser lamp 15, and a threaded hole is provided inside the laser lamp 15, which is adapted to the first lead screw 14. During use, the staff adds the sheath liquid from the water inlet pipe on the upper surface of the sheath liquid cover plate 4 into the sheath liquid barrel 3, and adds the test sample into the sample hopper 6 through the sample inlet plug 8 on the upper surface of the sample cover plate 7. The staff controls the test amount of the sample by rotating the sample dividing plug 9, and the sheath liquid in the sheath liquid barrel 3 flows into the second pipe 10 through the first pipe 5. The sample and the sheath liquid are mixed through the second pipe 10, and the first lead screw 14 is rotated by the moving motor 13 to move the laser lamp 15 until it moves to a fixed position. At the same position, different laser lamps 15 are used to detect the samples in the sheath liquid. The detected samples and sheath liquid flow into the waste liquid tank 11, which avoids the inconvenience of controlling the detection volume of each sample during the detection, resulting in inaccurate detection and unnecessary waste. It is impossible to perform grouped detection multiple times, it is inconvenient to move the laser lamp 15, and it is impossible to perform detection with different laser lamps 15. The detection is inconvenient and inefficient. Therefore, the detection volume is controlled by the sample dividing plug 9, and multiple groups of detections can be performed multiple times. The detection is more accurate and avoids waste of resources. Different laser lamps 15 are used for detection, and the detection is more comprehensive and diverse, which improves the detection efficiency.

[0027] like Figures 2 to 5As shown, in this embodiment, a placement ring 16 is connected to the other side of the main box body 1, and a cleaning component 17 is sleeved on the other side of the main box body 1. The cleaning component 17 includes a cleaning box 1701, a water pump 1702, a hose 1703, a nozzle 1704 and a battery 1705. A water inlet pipe is provided on the upper surface of the cleaning box 1701, and a water pump 1702 is connected to the inside of the cleaning box 1701. The upper end of the water pump 1702 is connected to the hose 1703. The material of the hose 1703 is TPE thermoplastic elastomer. TPE thermoplastic elastomer is an ultra-soft material that can be stretched and squeezed at will. It has good elasticity and flexibility and is suitable for making car wash water pipes and other products that require high elasticity and high tensile strength. One end of the hose 1703 is sleeved with a nozzle 1704, and a plurality of water outlet holes are provided on the lower surface of the nozzle 1704. A battery 1705 is provided on the upper surface of the cleaning box 1701. During use, cleaning agent is added to the cleaning box 1701 through the water inlet pipe on the upper surface of the cleaning box 1701, and the cleaning agent in the cleaning box 1701 is extracted into the hose 1703 through the water pump 1702 and sprayed out through the water outlet holes on the lower surface of the nozzle 1704. The staff holds the nozzle 1704 to spray the cleaning agent onto the sheath liquid barrel 3 for cleaning, and the cleaning agent flows from the first pipe 5 into the second pipe 10. The nozzle 1704 is used to spray the cleaning agent into the sample hopper 6 for cleaning. The detergent flows from the second pipe 10 to the waste liquid tank 11. By taking out the waste liquid tank 11 and opening the waste liquid cover 12 to pour out the cleaning agent, the staff can also clean the surface of the equipment with a handheld nozzle 1704, avoiding the inconvenience of cleaning for the staff after use, wasting working time, and reducing the service life of the equipment. If the equipment is not cleaned after long-term use, it is easy to cause reduced detection accuracy and affect work efficiency. Therefore, cleaning the equipment through the cleaning component 17 is convenient for the staff to use, improves the cleaning speed, keeps the equipment clean and does not deviate when the staff conducts inspection, extends the service life of the equipment, reduces working costs, and improves work efficiency.

[0028] This utility works as follows:

[0029] During use, the staff adds the sheath liquid into the sheath liquid barrel 3 from the water inlet pipe on the upper surface of the sheath liquid cover plate 4, adds the test sample into the sample hopper 6 through the sample inlet plug 8 on the upper surface of the sample cover plate 7, and controls the test amount of the sample by rotating the sample dividing plug 9. The sheath liquid in the sheath liquid barrel 3 flows into the second pipe 10 through the first pipe 5, and the sample and the sheath liquid are mixed through the second pipe 10. The first screw 14 is rotated by the moving motor 13 to move the laser lamp 15 to different positions, and different laser lamps 15 are used to perform sample analysis on the sample in the sheath liquid. The detected sample and sheath liquid flow into the waste liquid tank 11, which avoids the inconvenience of controlling the detection amount of the sample each time during the detection, resulting in inaccurate detection, unnecessary waste, and the inability to perform grouped detection multiple times. It is inconvenient to move the laser lamp 15, and it is impossible to perform detection of different laser lamps 15. The detection is inconvenient and the efficiency is low. Therefore, the detection amount is controlled by the sample dividing plug 9, and multiple groups of detections can be performed multiple times. The detection is more accurate and avoids waste of resources. Different laser lamps 15 are used for detection, and the detection is more comprehensive and diverse, which improves the detection efficiency and the equipment is used. After use, add cleaning agent to the cleaning box 1701 through the water inlet pipe on the upper surface of the cleaning box 1701, extract the cleaning agent in the cleaning box 1701 into the hose 1703 through the water pump 1702, and spray it out through the water outlet on the lower surface of the nozzle 1704. The staff holds the nozzle 1704 and sprays the cleaning agent to the sheath liquid barrel 3 for cleaning. The cleaning agent flows from the first pipe 5 to the second pipe 10. The nozzle 1704 is sprayed into the sample hopper 6 for cleaning. The cleaning agent flows from the second pipe 10 to the waste liquid tank 11. By taking out the waste liquid tank 11, open The waste liquid cover 12 pours out the cleaning agent after cleaning, and the staff can also clean the surface of the equipment with a handheld nozzle 1704, avoiding the inconvenience of cleaning for the staff after use, wasting working time, and reducing the service life of the equipment. If the equipment is not cleaned after long-term use, it is easy to cause reduced detection accuracy and affect work efficiency. Therefore, cleaning the equipment through the cleaning component 17 is convenient for the staff to use, improves the cleaning speed, keeps the equipment clean and does not deviate when the staff conducts inspection, thereby extending the service life of the equipment, reducing working costs, and improving work efficiency.

[0030] The electrical components mentioned in this article are all connected to an external main controller and 220V AC power, and the main controller can be a conventional known device that performs control such as a computer.

[0031] Finally, it should be noted that the above is only a preferred embodiment of the present invention and is not intended to limit the present invention. Although the present invention has been described in detail with reference to the aforementioned embodiments, those skilled in the art can still modify the technical solutions described in the aforementioned embodiments or make equivalent replacements for some of the technical features therein. Any modifications, equivalent replacements, improvements, etc. made within the spirit and principles of the present invention should be included in the scope of protection of the present invention.

Claims

1. A high-throughput flow fluorescence assay device, comprising a main housing (1), characterized in that: The upper surface of the main box (1) is connected to a box cover (2), the upper surface of the box cover (2) is connected to a sheath liquid barrel (3), the upper surface of the sheath liquid barrel (3) is connected to a sheath liquid cover (4), the lower surface of the sheath liquid barrel (3) is connected to a first pipe (5), the upper surface of the box cover (2) is connected to a sample hopper (6), the upper surface of the sample hopper (6) is connected to a sample cover (7), the upper surface of the sample cover (7) is connected to a sample inlet plug (8), a sample dividing plug (9) is threaded through one side of the sample hopper (6), and the sample The lower surface of the bucket (6) is plugged with a second pipe (10), one side of the main box (1) is sleeved with a waste liquid tank (11), the upper surface of the waste liquid tank (11) is clamped with a waste liquid cover (12), the inner wall of the main box (1) is provided with a moving motor (13), one end of the moving motor (13) is sleeved with a first lead screw (14), the inner wall of the main box (1) is slidably connected with a laser light (15), the other side of the main box (1) is plugged with a placement ring (16), and the other side of the main box (1) is sleeved with a cleaning component (17).

2. The high-throughput flow cytometry device according to claim 1, wherein: The cleaning assembly (17) comprises a cleaning box (1701), a water pump (1702), a hose (1703), a nozzle (1704) and a battery (1705). The water pump (1702) is plugged into the interior of the cleaning box (1701), the hose (1703) is plugged into the upper end of the water pump (1702), the nozzle (1704) is sleeved on one end of the hose (1703), and the battery (1705) is provided on the upper surface of the cleaning box (1701).

3. The high-throughput flow cytometry device according to claim 2, wherein: The lower surface of the nozzle (1704) is provided with a plurality of water outlet holes, and the upper surface of the cleaning box (1701) is provided with a water inlet pipe.

4. The high-throughput flow cytometry device according to claim 1, wherein: A threaded hole is provided inside the laser lamp (15), and the threaded hole and the first lead screw (14) are adapted to each other.

5. The high-throughput flow cytometry device according to claim 1, wherein: The upper surface of the sheath liquid cover plate (4) is provided with a water inlet pipe, and the upper surface of the box cover plate (2) is provided with a pipeline hole, and the pipeline hole is consistent in size with the first pipeline (5).

6. The high-throughput flow cytometry device according to claim 1, wherein: A hole is provided on one side of the second pipe (10), the hole being connected to the first pipe (5), and a pipe hole is provided on the upper surface of the box cover (2), the pipe hole being the same size as the second pipe (10).

7. The high-throughput flow cytometry device according to claim 1, wherein: A pipe hole is provided on one side of the main box body (1), and a hole is provided on the other side of the waste liquid tank (11), and the second pipe (10) passes through the waste liquid tank (11) through the pipe hole.

8. The high-throughput flow cytometry device according to claim 1, wherein: Bolts are threaded through the upper surface of the box cover (2), and the box cover (2) is threaded through the main box (1).

Images