High-precision caenorhabditis elegans nanoscale control and imaging platform

Abstract

The utility model belongs to the technical field of operating tables, and particularly relates to a high-precision caenorhabditis elegans nanoscale operating and imaging platform which comprises a connecting plate, telescopic legs which are evenly distributed are rotatably connected to the outer side of the connecting plate, supporting feet are rotatably connected to the inner sides of the bottom ends of the telescopic legs, an adjusting assembly is arranged at the top end of the connecting plate, and the adjusting assembly is connected with the connecting plate. The host assembly is arranged at the top end of the adjusting assembly. The utility model provides a high-precision caenorhabditis elegans nanoscale control and imaging platform which can be taken out by reducing the size of equipment, and meanwhile, the equipment is modularly designed, so that the platform is convenient to carry and brings multiple benefits, firstly, the flexibility is improved by the design, researchers can use the equipment in a natural environment outside a laboratory, and the working efficiency is improved. Therefore, the ecological correlation of research results is enhanced, behaviors and physiological reactions of the nematodes can be observed more truly, in addition, experiments under various environmental conditions become possible through modularization, and the depth and comprehensiveness of research are improved.

Description

Technical Field

[0001] The utility model relates to the technical field of operating platforms, in particular to a high-precision Caenorhabditis elegans nanoscale manipulation and imaging platform. Background Art

[0002] The high-precision Caenorhabditis elegans (Caenorhabditis elegans) nanoscale manipulation and imaging platform combines biology, engineering, and nanotechnology, providing a powerful tool for in-depth understanding of the microscopic mechanisms within organisms. As an important model organism, Caenorhabditis elegans is widely used in developmental biology, neuroscience, and drug screening due to its fully sequenced genome, simple developmental process, and transparency. Nanomanipulation technology achieves high-precision control of nematodes and their cells through methods such as laser capture and microfluidics, while advanced imaging technologies such as fluorescence microscopy and super-resolution microscopy enable researchers to observe the internal structure and dynamic processes of cells in real time. The combination of these technologies has not only advanced the understanding of cell differentiation and neuronal connectivity, but also opened up possibilities for emerging research such as gene editing. However, challenges such as manipulation precision and imaging speed still exist, and future development will focus on technology integration to obtain more comprehensive biological information.

[0003] However, in the actual use of existing schemes, most of them can only be used in laboratories or other fixed locations, and the high-precision Caenorhabditis elegans nanoscale manipulation and imaging platform is mainly used in laboratories or fixed locations. This limitation brings some potential problems. The difference between laboratory environment and natural conditions may cause the behavior and growth of nematodes to be inconsistent with the natural state, thereby affecting the ecological relevance of the research results. At the same time, manipulation and imaging in fixed positions limit the real-time observation of the dynamic behavior of nematodes and reduce the comprehensive understanding of biological processes. In addition, the number of samples processed in the laboratory is usually limited, which affects the validity of statistical analysis and the generalizability of the results. The high cost and professional maintenance requirements of high-precision equipment may also limit the accessibility of the laboratory, and fixed laboratory processes may lead to slow research progress, especially in experiments requiring long-term observation. Finally, the laboratory environment may not be able to fully capture the complex interactions between nematodes and microorganisms and other organisms. These factors may pose challenges to the depth and breadth of research.

[0004] To this end, the utility model provides a high-precision Caenorhabditis elegans nanoscale manipulation and imaging platform. Utility Model Content

[0005] In order to make up for the shortcomings of the existing technology and solve the problem that most of the existing technical solutions can only be used in fixed positions, the utility model proposes a high-precision Caenorhabditis elegans nanoscale manipulation and imaging platform.

[0006] The technical solution adopted by the present invention to solve its technical problems is: the high-precision Caenorhabditis elegans nanoscale manipulation and imaging platform described in the present invention includes a connecting plate, the outer side of the connecting plate is rotatably connected to evenly distributed telescopic legs, the inner side of the bottom end of the telescopic leg is rotatably connected to a supporting foot, the top of the connecting plate is provided with an adjustment component, and the top of the adjustment component is provided with a mainframe component.

[0007] Furthermore, the adjustment assembly includes a base plate, the top of the connecting plate is fixedly connected to the base plate, the top of the base plate is fixedly connected to a uniformly distributed first articulated frame, the top of the first articulated frame is rotatably connected to a telescopic rod, the top of the telescopic rod is rotatably connected to a second articulated frame, and the top of the second articulated frame is fixedly connected to a top plate.

[0008] Furthermore, the host assembly includes a host case, the host case is provided on the top of the top plate, and a handle is rotatably connected to the middle side of the front end of the host case.

[0009] Furthermore, the bottom end of the top plate is fixedly connected to left and right opposite fixing rods, the bottom end of the fixing rods is fixedly connected to a support plate, the top end of the support plate is fixedly connected to a limiting sleeve, the inner side of the middle end of the limiting sleeve is slidably connected to evenly distributed limiting balls, and a sliding component is provided on the outer side of the limiting sleeve.

[0010] Furthermore, the sliding assembly includes a locking sleeve, a locking sleeve is slidably connected to the outer side of the limiting sleeve, a locking ring is fixedly connected to the inner side of the middle end of the locking sleeve, the locking ring is engaged with the limiting ball, an extended range chamber is provided at the top end of the inner side of the locking sleeve, a spring is provided on the outer side of the middle end of the limiting sleeve, and the top end of the spring is fixedly connected to the locking ring.

[0011] Furthermore, a locking column is fixedly connected to the middle side of the bottom end of the main chassis, and a locking groove is opened at the middle end of the locking column, and the locking groove is locked and connected with the locking ring.

[0012] Furthermore, a gyroscope is fixedly connected to the inner top end of the engaging column.

[0013] Furthermore, the outer wall of the main box is provided with a rubber layer.

[0014] The beneficial effects of the utility model are as follows:

[0015] 1. The high-precision Caenorhabditis elegans nanoscale manipulation and imaging platform described in the present invention reduces the size of the device so that it can be taken out, and modularizes it for portability, which brings multiple benefits. First, this design improves flexibility, allowing researchers to use the device in natural environments outside the laboratory, thereby enhancing the ecological relevance of the research results and enabling more realistic observation of the behavior and physiological responses of nematodes. In addition, modularity makes it possible to conduct experiments under a variety of environmental conditions, obtain a wider range of data, and enhance the depth and comprehensiveness of the research. It also promotes academic exchanges and cooperation, because the equipment can be easily transported to different research teams, simplifying the maintenance and upgrade process, and introducing new technologies in a timely manner. At the same time, researchers can freely combine different modules according to specific needs to achieve flexible experimental design, reduce unnecessary costs, and optimize resource allocation.

[0016] 2. The high-precision Caenorhabditis elegans nanoscale manipulation and imaging platform described in the utility model adjusts the angle of the top plate by arranging multiple telescopic rods at different angles on the top of the base plate to cooperate with each other, thereby adjusting the horizontal angle and posture of the main chassis. The solution with adjustable horizontal angle and posture of the main chassis has multiple advantages, including improved manipulation accuracy and enhanced control over the position and posture of the nematodes, thereby improving the reliability of experimental data. At the same time, the adjustment angle supports multi-angle imaging, obtaining more comprehensive visual information, and facilitating in-depth analysis of nematode behavior. This design also enhances experimental flexibility, reduces external interference, optimizes the observation experience, and expands the application range of the platform, making it compatible with more experimental equipment. BRIEF DESCRIPTION OF THE DRAWINGS

[0017] Figure 1 It is a schematic diagram of the three-dimensional structure of the utility model;

[0018] Figure 2 This is a schematic diagram of the three-dimensional structure of the utility model Figure 1 and enlarged images;

[0019] Figure 3 It is a schematic diagram of a partial three-dimensional structure of the utility model;

[0020] Figure 4 This is a schematic diagram of the cross-sectional structure of the limiting sleeve in the utility model;

[0021] Figure 5 It is a partial three-dimensional structural diagram of the main chassis of the utility model;

[0022] In the figure: 1, connecting plate; 11, telescopic leg; 12, supporting foot; 2, bottom plate; 21, first hinged frame; 22, telescopic rod; 23, second hinged frame; 24, top plate; 25, fixed rod; 26, supporting plate; 3, limiting sleeve; 31, limiting ball; 32, clamping sleeve; 33, clamping ring; 34, range increasing chamber; 35, spring; 4, clamping column; 41, clamping groove; 42, gyroscope; 5, main box; 51, handle. DETAILED DESCRIPTION

[0023] To make the possible application scenarios, technical principles, specific schemes that can be implemented, purposes and effects achieved, etc. of the present application clear, the following will be described in detail in combination with the specific embodiments listed and the accompanying drawings. The embodiments described herein are only used to more clearly illustrate the technical schemes of the present application, and therefore only serve as examples, but cannot limit the protection scope of the present application.

[0024] In this paper, the term "embodiment" means that the specific features, structures or characteristics described in combination with the embodiment can be included in at least one embodiment of the present application. The term "embodiment" appearing at various positions in the specification does not necessarily refer to the same embodiment, and does not particularly limit the independence or association between other embodiments. In principle, in the present application, as long as there is no technical contradiction or conflict, the technical features mentioned in each embodiment can be combined in any way to form a corresponding implementable technical scheme.

[0025] Unless otherwise defined, the meaning of the technical terms used herein is the same as that generally understood by those skilled in the art to which the present application belongs; the use of related terms herein is only for the purpose of describing specific embodiments, and is not intended to limit the present application.

[0026] In the description of the present application, the word "and / or" is a description of the logical relationship between the objects, which means that there can be three relationships, for example, A and / or B, which means that there are three cases: A exists, B exists, and A and B exist at the same time. In addition, the character " / " in this paper generally represents that the associated objects before and after are a kind of "or" logical relationship.

[0027] In the present application, such as "first" and "second", the terms are only used to distinguish one entity or operation from another entity or operation, and do not necessarily require or imply any actual quantity, primary and secondary or order relationship between the entities or operations.

[0028] Without further limitations, in this application, the words "include", "comprise", "have" or other similar expressions used in the sentences are intended to cover non-exclusive inclusion. These expressions do not exclude the presence of additional elements in the process, method or product including the elements, so that the process, method or product including a series of elements may include not only those defined elements, but also other elements not explicitly listed, or elements inherent to such process, method or product.

[0029] Consistent with the understanding in the Examination Guidelines, in this application, expressions such as "greater than," "less than," and "exceed" are understood to exclude the number itself; expressions such as "above," "below," and "within" are understood to include the number itself. Furthermore, in the description of the embodiments of this application, "multiple" means more than two (including two), and similar expressions related to "multiple" are also understood in this manner, such as "multiple groups," "multiple times," etc., unless otherwise specifically defined.

[0030] In the description of the embodiments of the present application, the space-related expressions used, such as "center", "longitudinal", "lateral", "length", "width", "thickness", "up", "down", "front", "back", "left", "right", "vertical", "horizontal", "vertical", "top", "bottom", "inside", "outside", "clockwise", "counterclockwise", "axial", "radial", "circumferential", etc., indicate the orientation or position relationship based on the orientation or position relationship shown in the specific embodiments or drawings, and are only for the convenience of describing the specific embodiments of the present application or facilitating the reader's understanding, and do not indicate or imply that the device or component referred to must have a specific position, a specific orientation, or be constructed or operated in a specific orientation. Therefore, it should not be understood as a limitation on the embodiments of the present application.

[0031] Unless otherwise expressly specified or limited, in the description of the embodiments of the present application, the terms "installed", "connected", "connected", "fixed", "set", etc. used should be understood in a broad sense. For example, the "connection" can be a fixed connection, a detachable connection, or an integrated setting; it can be a mechanical connection, an electrical connection, or a communication connection; it can be a direct connection or an indirect connection through an intermediate medium; it can be the internal connection of two elements or the interaction relationship between two elements. For those skilled in the art of the present application, the specific meanings of the above terms in the embodiments of the present application can be understood according to the specific circumstances.

[0032] Example 1:

[0033] like Figures 1 to 5The utility model discloses a high-precision caenorhabditis elegans nanometer level manipulation and imaging platform, including connecting plate 1, the connecting plate 1 outside rotationally connected with the evenly distributed telescopic leg 11, the top of telescopic leg 11 is limited to connecting plate 1, and the bottom end inside rotationally connected with support foot 12 of telescopic leg 11 is limited to support foot 12 by telescopic leg 11, and the top of connecting plate 1 is provided with adjusting assembly, and the top of adjusting assembly is provided with host computer assembly.

[0034] Adjusting assembly includes bottom plate 2, and the top of connecting plate 1 is fixedly connected with bottom plate 2, and bottom plate 2 is supported and fixed by connecting plate 1, and the top of bottom plate 2 is fixedly connected with the evenly distributed first hinged frame 21, and a plurality of first hinged frames 21 are supported and fixed by bottom plate 2, and the top of first hinged frame 21 is rotatably connected with telescopic rod 22, and the bottom end of telescopic rod 22 is limited by first hinged frame 21, and the top of telescopic rod 22 is rotatably connected with second hinged frame 23, and the top of second hinged frame 23 is fixedly connected with top plate 24, and the top of telescopic rod 22 and top plate 24 are connected by second hinged frame 23, and telescopic rod 22 in different directions is telescoped simultaneously, so as to drive the horizontal attitude of top plate 24 to be adjusted.

[0035] Host computer assembly includes host computer box 5, and the top of top plate 24 is provided with host computer box 5, and host computer box 5 is supported by top plate 24, and the front end of host computer box 5 is rotatably connected with handle 51, and handle 51 is limited by host computer box 5, and host computer box 5 can be taken away by handle 51.

[0036] The bottom of top plate 24 is fixedly connected with the fixed rod 25 opposite left and right, and the fixed rod 25 is fixed by top plate 24, and the bottom of fixed rod 25 is fixedly connected with support plate 26, and support plate 26 is fixed by fixed rod 25, and the top of support plate 26 is fixedly connected with the limiting sleeve 3, and the limiting sleeve 3 is fixed by support plate 26, and the limiting sleeve 3 is slidably connected with the evenly distributed limiting ball 31 in the middle end inside, and the limiting ball 31 is limited by limiting sleeve 3, and the limiting sleeve 3 is provided with sliding assembly outside.

[0037] The sliding assembly includes a locking sleeve 32, and the locking sleeve 32 is slidably connected to the outer side of the limiting sleeve 3, and the locking sleeve 32 is limited by the limiting sleeve 3, and the locking ring 33 is fixedly connected to the inner side of the middle end of the locking sleeve 32, and the locking ring 33 is fixed by the locking sleeve 32. The locking ring 33 is locked and connected with the limiting ball 31, and the stroke of the limiting ball 31 is controlled by the locking ring 33. An extended range chamber 34 is provided at the top inner side of the locking sleeve 32, and the extended range chamber 34 is provided on the inner side of the locking sleeve 32 to increase the stroke of the locking ring 33 moving outward. A spring 35 is provided on the outer side of the middle end of the limiting sleeve 3, and the top end of the spring 35 is fixedly connected to the locking ring 33. The spring 35 uses the outer bottom end of the limiting sleeve 3 as the stress point to push the locking ring 33 upward, thereby supporting and fixing the position of the locking ring 33.

[0038] A locking column 4 is fixedly connected to the middle side of the bottom end of the main chassis 5, and the locking column 4 is fixed by the main chassis 5. A locking groove 41 is opened at the middle end of the locking column 4, and the locking groove 41 is locked and connected with the locking ring 33.

[0039] A gyroscope 42 is fixedly connected to the top inner side of the snap-fit ​​column 4 . The gyroscope 42 is fixed by the snap-fit ​​column 4 , and the horizontal posture of the main chassis 5 can be adjusted by the gyroscope 42 .

[0040] The outer wall of the main chassis 5 is provided with a rubber layer. By providing the rubber layer on the outside of the main chassis 5, the main chassis 5 itself is protected.

[0041] Working principle: When the high-precision Caenorhabditis elegans nanoscale manipulation and imaging platform is in operation, first, the telescopic legs 11 are unfolded outwards to adjust the length of the telescopic legs 11, and then the engaging sleeve 32 is pulled downwards, and the engaging ring 33 and the range extension chamber 34 are driven to move downwards at the same time by the engaging sleeve 32, and the engaging limit of the engaging ring 33 on the limiting ball 31 is cancelled. Then, the engaging column 4 at the bottom of the main case 5 is inserted into the limiting sleeve 3, and then the engaging sleeve 32 is released, and the engaging ring 33 is pushed upwards by the spring 35 with the limiting sleeve 3 as the stress point, and at the same time At the same time, the locking sleeve 32 and the range extension chamber 34 are driven to move upward by the locking ring 33, and the upward movement of the locking ring 33 squeezes the limit ball 31, forcing the locking ring 33 to move inward and engage with the locking groove 41, thereby fixing the locking column 4 and the limit sleeve 3, completing the fixation of the main chassis 5, and then the multiple telescopic rods 22 cooperate with each other, and the horizontal posture of the main chassis 5 is monitored by the gyroscope 42, so as to control the telescopic rods 22 at different positions to extend and retract, thereby adjusting the horizontal posture of the main chassis 5.

[0042] Finally, it should be noted that the above embodiments have been described in the specification and drawings of the application, but this does not limit the patent protection scope of the application. Any equivalent structure or equivalent process replacement or modification based on the essential concept of the application, using the content described in the specification and drawings of the application, and directly or indirectly implementing the technical solutions of the above embodiments in other related technical fields, etc., are all included in the patent protection scope of the application.

Claims

1. A high-precision Caenorhabditis elegans nanoscale manipulation and imaging platform, characterized by: It includes a connecting plate, the outer side of which is rotatably connected to evenly distributed telescopic legs, the inner side of the bottom end of the telescopic legs is rotatably connected to support feet, the top of the connecting plate is provided with an adjustment component, and the top of the adjustment component is provided with a mainframe component.

2. The high-precision Caenorhabditis elegans nanoscale manipulation and imaging platform according to claim 1, characterized in that: The adjustment assembly includes a base plate, the top of the connecting plate is fixedly connected to the base plate, the top of the base plate is fixedly connected to a uniformly distributed first articulated frame, the top of the first articulated frame is rotatably connected to a telescopic rod, the top of the telescopic rod is rotatably connected to a second articulated frame, and the top of the second articulated frame is fixedly connected to a top plate.

3. The high-precision Caenorhabditis elegans nanoscale manipulation and imaging platform according to claim 2, characterized in that: The host assembly includes a host box, the top of the top plate is provided with the host box, and the middle side of the front end of the host box is rotatably connected with a handle.

4. The high-precision Caenorhabditis elegans nanoscale manipulation and imaging platform according to claim 3, characterized in that: The bottom end of the top plate is fixedly connected to the left and right opposite fixing rods, the bottom end of the fixing rod is fixedly connected to the support plate, the top end of the support plate is fixedly connected to the limiting sleeve, the inner side of the middle end of the limiting sleeve is slidably connected to the evenly distributed limiting balls, and the outer side of the limiting sleeve is provided with a sliding component.

5. The high-precision Caenorhabditis elegans nanoscale manipulation and imaging platform according to claim 4, characterized in that: The sliding assembly includes a locking sleeve, a locking sleeve is slidably connected to the outer side of the limiting sleeve, a locking ring is fixedly connected to the inner side of the middle end of the locking sleeve, the locking ring is engaged with the limiting ball, an extended range chamber is opened at the top end of the inner side of the locking sleeve, a spring is provided on the outer side of the middle end of the limiting sleeve, and the top end of the spring is fixedly connected to the locking ring.

6. The high-precision Caenorhabditis elegans nanoscale manipulation and imaging platform according to claim 5, characterized in that: A locking column is fixedly connected to the middle side of the bottom end of the main chassis, and a locking groove is opened at the middle end of the locking column, and the locking groove is locked and connected with the locking ring.

7. The high-precision Caenorhabditis elegans nanoscale manipulation and imaging platform according to claim 6, characterized in that: The top end of the inner side of the locking column is fixedly connected with a gyroscope.

8. The high-precision Caenorhabditis elegans nanoscale manipulation and imaging platform according to claim 7, characterized in that: The outer wall of the main box is provided with a rubber layer.

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