Flow cell lens adhering device

Abstract

The utility model relates to the field of lens bonding, and discloses a flow cell lens bonding device which comprises a tool bottom plate and a fluid chamber assembling component, a detachable positioning seat is installed on the tool bottom plate, the positioning seat comprises a seat body connected with the tool bottom plate, a positioning hole is formed in the top face of the seat body, and a positioning component is arranged at one end of the positioning seat; the fluid chamber assembling component comprises a fluid chamber body, a flow cell body is mounted at the top of the fluid chamber body, and a concave reflector is mounted on one side of the flow cell body; the side face of the fluid chamber body is connected with the baffle, the flow cell body is assembled on the fluid chamber assembling assembly and then bonded with the aspheric lens, the top face of the fluid chamber body and the inner side face of the baffle serve as the benchmark and are attached to the two adjacent faces of the base body respectively, the structure is simplified, the hand is prevented from making contact with the flow cell body, and the service life of the flow cell body is prolonged. And meanwhile, the final realization effect of the assembly body is ensured.

Description

TECHNICAL FIELD

[0001] The utility model relates to the field of lens bonding, specifically relates to a flow cell lens bonding device. BACKGROUND

[0002] The flow cell of the current flow cytometer is the core optical part of fluid focusing and is also the core flow path part. Due to the difference of optical path systems designed by various manufacturers, some need to bond optical lenses at the lateral position of the flow cell focusing to meet the optimal collection of lateral fluorescence. For example, some need to bond a non-spherical lens on one side of the flow cell and bond a concave mirror on the opposite side; and some flow cells are bonded with a concave mirror on one side by the supplier before leaving the factory, so a simple manual tool needs to be designed to realize the manual bonding of the non-spherical lens.

[0003] The diameter of the concave mirror is 10.6 mm, which is bonded at the center position of the wide side of the flow cell (a cube made of quartz glass with a small rectangular flow channel in the middle, the cross section is two narrow sides and two wide sides). The diameter of the non-spherical lens is 11 mm, which needs to be bonded on the opposite side of the flow cell opposite to the concave mirror. The width of the side of the flow cell is 10 mm. Both of the bonded lenses are slightly larger than the width of the side of the flow cell, and the position of the non-spherical lens is required to be coaxial with the concave mirror or at the same center position of the flow cell. The final assembly of the flow cell is to bond it on the top of the fluid chamber, so that the center height of the non-spherical lens meets the required optical axis height.

[0004] The existing conventional lens bonding method is as follows:

[0005] (1) Coaxial horizontal arrangement (the bonding plane is perpendicular to the horizontal plane), fix the two bonding parts on the same sliding rail to ensure coaxial relative movement, which is easy to realize in structure. However, the glue dispensing link in this scheme is difficult to control, and the glue is easy to overflow downward or cause uneven glue layer;

[0006] (2) Bonding plane arrangement up and down:

[0007] l With the flow cell fixed, the bonding surface of the flow cell faces upward, and the concave mirror is positioned downward in a circle;

[0008] l With the flow cell fixed, the bonding surface of the flow cell faces upward, and the rectangular side of the flow cell is positioned;

[0009] The above structure is designed as a positioning seat (circular hole or rectangular hole) at the lower part and a pressing block at the upper part. The lens is put into the circular hole, and the upper and lower parts are matched with a guide shaft to complete the assembly. Although the positioning is accurate, the structure is complicated to install and disassemble. In addition, due to the coverage and obstruction of the structure, it is not conducive to the ultraviolet lamp irradiation curing and the removal of the overflowed glue;

[0010] (3) The glue plane is arranged up and down to fix the aspheric lens to be adhered, and the circumference of the lens is positioned;

[0011] The lower layer is positioned by the circumference of the aspheric lens, and the upper layer is positioned by the shape of the flow cell.

[0012] The structure of the method (1) is simple and convenient to operate, has a larger operation space, and has less contact with the operator's hand, but the glue injection may be uneven due to gravity; the structure of the method (2) has high reliability and is a commonly used method, but the structure is complicated to install and disassemble, and lacks operation space for observing and removing excess glue; the structure of the method (3) is simple and convenient to operate, but it is difficult to separate the parts from the tool after the glue is cured, and the operator's hand is inevitably in contact with the parts.

[0013] For the above several conventional methods, the one-time positioning of the shape is adopted in the positioning of the parts, and the positioning accuracy is required to be high, so that it is difficult to separate the parts from the tool after the glue is adhered. At the same time, the operator's hand is inevitably in contact with the surface of the parts during operation, which affects the optical effect of the parts; the amount of glue used for manual glue injection will be more to ensure that there is no air bubble in the glue layer, and excess glue will inevitably overflow during compression. After the ultraviolet lamp is irradiated, the excess glue must be removed in time, so that the tool structure must avoid enough space; when removing the excess glue, the adhered lens will also be forced to cause a small deviation of the adhered position. Content of the utility model

[0014] The utility model aims at overcoming the defects in the prior art, providing a flow cell lens sticking device, which reduces the difficulty of separating the parts from the tool and reduces the contact of the hand with the parts while ensuring the precision of the adhered position; secondly, manual glue injection ensures that the position does not deviate when removing the excess glue, and meets the demand of operation space.

[0015] To achieve the above-mentioned purpose, the utility model adopts the technical scheme that:

[0016] A flow cell lens sticking device comprises a tooling base plate and a fluid chamber assembly component, wherein a detachable positioning seat is mounted on the tooling base plate, the positioning seat comprises a seat body connected to the tooling base plate, the top surface of the seat body is provided with a positioning hole matching the outer diameter of the aspheric lens, and one end of the positioning seat is provided with a positioning assembly for fixing the fluid chamber assembly component; the fluid chamber assembly component comprises a fluid chamber body, the top of the fluid chamber body is provided with a flow cell body, and one side of the flow cell body is provided with a concave reflector; the side of the fluid chamber body is connected to a baffle, the baffle is perpendicular to the top surface of the fluid chamber body, and when the baffle and the fluid chamber body are both abutted against the outer side of the seat body, the concave reflector is coaxial with the positioning hole.

[0017] Optionally, the positioning assembly includes a support plate parallel to the baffle, the support plate is located at one end of the positioning seat, and a lateral adjustment positioning bead for fixing the baffle is passed through the support plate.

[0018] Optionally, a mounting hole is provided on the support plate, and the lateral adjustment positioning bead is screwed and passed through the mounting hole.

[0019] Optionally, the mounting holes are threaded holes, and there are at least two mounting holes.

[0020] Optionally, a pressure plate is fixedly installed on the inner side of the baffle, and a waste liquid connector is passed through the pressure plate; two positioning bosses are relatively provided on the top of the fluid chamber body, one end of the flow pool body is embedded between the two positioning bosses, and the other end is abutted against the waste liquid connector.

[0021] Optionally, opposite through grooves are formed on both sides of the positioning hole.

[0022] Optionally, an inner hole is formed at the bottom end of the positioning hole, and the inner hole is coaxial with the positioning hole.

[0023] Optionally, a connecting hole is provided on the base body, and one end of the fastener passes through the connecting hole and is screwed into the tooling base plate.

[0024] Compared with the prior art, the beneficial effects of the present invention are as follows:

[0025] (1) In the present invention, the flow cell body is assembled on the fluid chamber assembly before the aspheric lens is bonded. The top surface of the fluid chamber body and the inner side surface of the baffle are used as references to fit the two adjacent surfaces of the base body respectively. This not only simplifies the structure but also avoids the contact of the hand with the flow cell body, while ensuring the final effect of the assembly. In addition, one of the two fixed positions is converted into an adjustable position, so that the separation of the parts and the tooling can be easily achieved.

[0026] (2) In the present invention, the grooves on both sides of the positioning hole are convenient for using a cotton swab to remove the overflowed glue, and the inner hole is convenient for locating the height of the protruding aspheric surface, and at the same time plays a role in adaptive centering of the spherical surface. BRIEF DESCRIPTION OF THE DRAWINGS

[0027] Figure 1 This is a schematic structural diagram of a flow cell lens bonding device according to an embodiment of the present invention;

[0028] Figure 2 This is a schematic structural diagram of a positioning seat in an embodiment of the present utility model;

[0029] Figure 3 This is a schematic structural diagram of the fluid chamber assembly component in an embodiment of the present utility model;

[0030] Figure 4 This is a schematic diagram of the main structure of the fluid chamber assembly in an embodiment of the present utility model;

[0031] Figure 5 This is a schematic top view of the structure of the flow cell lens bonding device according to an embodiment of the present utility model;

[0032] Among them, 1. tooling base plate;

[0033] 2. Positioning seat; 201. Seat body; 202. Positioning hole; 203. Through slot; 204. Inner hole; 205. Connecting hole;

[0034] 3. Support plate; 301. Mounting hole; 4. Lateral adjustment positioning beads; 5. Flow cell body; 6. Concave reflector; 7. Aspheric lens; 8. Positioning boss; 9. Fluid chamber body; 10. Base; 11. Baffle; 12. Pressure plate; 13. Waste liquid connector. DETAILED DESCRIPTION

[0035] The present invention will now be described in further detail with reference to the accompanying drawings and embodiments. These drawings are simplified schematic diagrams that only illustrate the basic structure of the present invention in a schematic manner, and therefore only show components related to the present invention.

[0036] The glue used to bond the flow cell lens is cured by using a special UV glue and UV light. The key here is: precise positioning of the flow cell body 5 and the aspheric lens 7 before bonding; manual dispensing and cleaning of overflowing glue, during which the relative positions of the bonded parts must be kept unchanged, and the bonding effect must be ensured (firm bonding, no bubbles or impurities on the bonding surface); after bonding and curing, the bonded parts must be separated from the operating tooling (the relative positions of the bonded parts must still be kept unchanged).

[0037] Existing lens gluing methods usually face two difficulties: one is the problem of manual glue dispensing and glue overflow. In order to ensure that the glue covers the entire bonding surface and no bubbles are mixed in, the amount of glue must be relatively large; the other is that the part size is small, and the part needs to be accurately positioned, so it fits tightly with the tooling. After the UV glue is cured, the part and the tooling are difficult to separate.

[0038] like Figure 1-Figure 5 As shown, a flow cell lens gluing device includes a tooling base plate 1, a positioning seat 2, a flow cell body 5, a concave reflector 6, an aspheric lens 7, a fluid chamber assembly component and a positioning assembly, etc. The positioning seat 2 can be detachably mounted on the tooling base plate 1, and plays a role in receiving and positioning the aspheric lens 7. The flow cell body 5 is mounted on the fluid chamber assembly component, and the concave reflector 6 is fixedly mounted on the side of the flow cell body 5 and is located at its center position; the positioning assembly is used to fix the fluid chamber assembly component on the tooling base plate 1, and can convert one of the two fixed positions required for gluing the lens into an adjustable position, so that the separation of parts and tooling can be easily achieved.

[0039] Before using the tooling, glue the flow cell body 5 and the fluid chamber assembly together with glue. This operation is also required in the actual assembly process. Here, in order to avoid touching the flow cell body 5 with hands during the gluing process, the two assembly surfaces of the fluid chamber assembly components are used to form a rectangular coordinate system for positioning; positioning with the assembly body can also effectively ensure the optical axis height of the lens; in the gluing process, in addition to the tooling body, some auxiliary materials are also required: ultraviolet lamp, pointed cotton swab, anhydrous alcohol, dust-free paper, and plastic tweezers.

[0040] The tooling base plate 1 is made of aluminum alloy, which has good flatness and serves as a reference plane for the tooling. The positioning component is set on the tooling base plate 1 and can adjust the horizontal X-axis coordinate of the fluid chamber assembly component.

[0041] The lens positioning seat 2 includes a seat body 201 connected to the tooling base plate 1, and a positioning hole 202 matching the outer diameter of the aspheric lens 7 is provided on the top surface of the seat body 201; opposite through grooves 203 are provided on both sides, and an inner hole 204 is provided at the bottom end of the positioning hole 202, and the inner hole 204 is coaxial with the positioning hole 202, that is, the positioning hole 202 and the inner hole 204 are a stepped hole structure as a whole.

[0042] The base 201 is made of POM (Polyoxymethylene) engineering plastic to prevent imprinting during lens compression. The slots on either side of the positioning hole 202 facilitate the removal of glue spills with a cotton swab. The inner hole 204 facilitates the positioning of the protruding aspheric surface, also providing adaptive centering of the circular hole to the spherical surface. The height of the aspheric lens's bonding surface has been set to match the height of the flow cell body 5's bonding surface after the fluid chamber assembly is placed on the tooling base 1, facilitating compression after glue application and controlling the film's thickness.

[0043] The base 201 is provided with a connection hole 205, and one end of a fastener is threaded through the connection hole 205 and screwed into the tooling base 1. The fastener is preferably a locking screw. The tooling base 1 is provided with threaded holes corresponding in number and position to the connection holes 205. Once the base 201 is in place, the locking screw is threaded from top to bottom through the connection hole 205 and into the threaded hole to secure the base 201 to the tooling base 1.

[0044] The fluid chamber assembly component includes a fluid chamber body 9, a baffle 11 is connected to the side of the fluid chamber body 9, a pressure plate 12 is fixedly installed on the inner side of the baffle 11, and a waste liquid connector 13 is passed through the pressure plate 12; a base 10 is installed at the bottom of the fluid chamber body 9, and two positioning bosses 8 are relatively arranged on the top of the fluid chamber body 9, and one end of the flow pool body 5 is embedded between the two positioning bosses 8, and the other end is in contact with the waste liquid connector 13.

[0045] The baffle 11 is perpendicular to the top surface of the fluid chamber body 9 , and when the baffle 11 and the fluid chamber body 9 are both against the outer side of the base 201 , the concave reflector 6 is coaxial with the positioning hole 202 .

[0046] Specifically, the flow pool body 5 is fixed in the horizontal center position in the positioning groove on the top of the fluid chamber body 9 (i.e., between the two positioning bosses 8) with glue, so that the positioning in the Y-axis direction can be determined by the cooperation between the top surface of the fluid chamber body 9 and the positioning seat 2; at the same time, the horizontal cross-section of the fluid chamber body 9 is a square, and the size is also a fine-machined size. With the help of the plane of the tooling base 1 and the height of the positioning seat 2, the position of the bonding plane can be determined in the Z-axis direction; in order to simplify the tooling structure, after the X-axis direction is limited by the positioning assembly, the Y-axis direction can be manually pressed and the Z-axis downward pressing of the adhesive surface can be achieved.

[0047] The positioning assembly includes a support plate 3 parallel to the baffle 11. The support plate 3 is located at one end of the positioning seat 2 and is fitted with lateral adjustment positioning beads 4 for securing the baffle 11. The support plate 3 has mounting holes 301 formed therein, into which the lateral adjustment positioning beads 4 are threaded and inserted. These mounting holes 301 are threaded and are provided with at least two of them. These lateral adjustment positioning beads 4 can tighten or loosen the fluid chamber assembly in the X-axis direction, allowing for easy positioning of the flow cell body 5 along the X-axis when separated from the fixture.

[0048] A positioning bead is typically a small mechanical component, also known as a ball plunger or pin. It consists of a body (typically a cylindrical housing), a spring, and a steel ball (or other material). The working principle is that the spring force partially pushes the ball out of the body. When positioning is required, the ball fits into a corresponding hole, groove, or recessed portion of another surface, achieving the desired positioning function.

[0049] After gluing, testing with a secondary element device reveals that the glue is glued in a tight state in both the X and Y axes, and there is a slight elastic recovery after release. At this point, negative compensation can be made in both directions of the positioning seat 2. This modification will ensure more accurate positioning of the tooling and more stable bonding dimensions after stress release.

[0050] The workflow for lens bonding using the flow cell lens bonding device proposed by the present invention is as follows.

[0051] (1) Fluid chamber assembly: First, connect the fluid chamber body 9 and the base 10 with positioning pins and screws. There are two positioning bosses 8 on the top of the fluid chamber body 9. Its width is the same as that of the flow cell body 5. Its position is centered relative to the fluid chamber body 9, thereby ensuring that the position of the flow cell body 5 is centered relative to the fluid chamber body 9; then install the baffle 11 and the pressure plate 12, and assemble the waste liquid connector 13 on the pressure plate 12, so that the waste liquid connector 13 presses the flow cell body 5 against the top surface of the fluid chamber body 9. In this way, the Y-axis position of the bonding lens can be determined with the help of the top surface of the fluid chamber body 9; Figure 4 As shown, reference plane B (the top surface of the fluid chamber body 9) and reference plane A (the inner side of baffle 11) can also be used to determine the X-axis position of the bonding lens. With this structure, reference planes A and B of the assembly can be used to determine the position of the lens to be bonded, without requiring direct manual contact with the flow cell body 5.

[0052] (2) Assembly of the mirror sticking fixture: Fix the seat body 201 of the positioning seat 2 to the fixture base plate 1 with locking screws, with the two sides of the seat body 201 coplanar with the fixture base plate 1; then screw the lateral adjustment positioning bead 4 into the mounting hole 301 of the support plate 3 as shown in the figure, and its head does not need to be screwed out for the time being.

[0053] (3) Place the aspheric lens 7: Use plastic tweezers to place the aspheric lens 7 into the positioning hole 202, with the bonding surface (plane) facing upward and the aspheric surface facing the inner hole 204. The height of the upper plane of the inner hole 204 is calculated so that the bonding surface height of the aspheric lens 7 after placement is equal to the height of the bonding surface of the flow cell body 5 after assembly and placement. Before gluing, wipe the bonding surface of the aspheric lens 7 clean with anhydrous alcohol. Similarly, the bonding surface of the flow cell body 5 also needs to be wiped clean with anhydrous alcohol.

[0054] (4) Glue fixation: According to the prescribed glue dispensing amount, use a pipette to adjust the sample suction volume, apply UV glue to the bonding plane of the aspheric lens 7, and then place the fluid chamber assembly on the tooling base 1; first manually align the reference plane A and reference plane B, then gently press the fluid chamber assembly with your hands, gradually increase the downward pressure until it is pressed together, and then use the lateral adjustment positioning beads 4 to press the fluid chamber assembly tightly; then first irradiate with UV light, and then use a cotton swab dipped in alcohol to carefully clean the glue overflow on the left and right sides to avoid affecting the light-transmitting surfaces on the left and right sides of the flow cell body 5.

[0055] (5) Disconnect the tooling:

[0056] First, still press the fluid chamber assembly onto reference planes A and B by hand and loosen the lateral adjustment positioning beads 4;

[0057] Secondly, try to maintain the pressure on your hands, release the pressure appropriately, and make sure the part can be smoothly removed from the tooling in the Z-axis direction. Do not shake it hard to cause position deviation.

[0058] Then, remove the glued flow cell and check the glue condition and visually inspect the workpiece bonding position; you can also reinstall the parts into the fixture and observe whether there is a large gap between the two reference planes without force; place the glued parts horizontally with the aspheric lens 7 on top for 24 hours to ensure the glue curing time;

[0059] Finally, in order to further reduce the position offset of the bonding, the two-dimensional element is used to measure the parts to obtain the actual deviation, and the two positioning surface dimensions of the positioning seat 2 on the tooling are corrected again, such as Figure 5 The two planes marked by the arrows can make the mirror position more accurate.

[0060] In summary, the flow cell lens sticking device proposed in this utility model mainly solves the following problems:

[0061] On the tooling positioning of the two parts to be bonded, one of them is set as a detachable positioning to facilitate separation of the part from the tooling after the bonding is cured; direct hand contact can be avoided when the part is placed in or separated from the tooling; sufficient operating space is provided for cleaning overflowed glue, and the offset caused when the part is separated from the tooling after the bonding is cured can be minimized, or the existing displacement can be compensated and corrected.

[0062] The structure of this device is relatively simple and easy to operate; one of the two fixed positionings is converted into an adjustable positioning, so that the separation of parts and tooling can be easily achieved; with the help of the structure of the actual assembly, a reasonable reference surface is selected as the positioning reference, and the positioning of the flow pool by the existing assembly replaces the tooling positioning, which also avoids hand contact with the flow pool, simplifies the structure, and ensures the final implementation effect of the assembly; since the positioning of the flow pool is reflected in the assembly with the fluid chamber, there is little structural obstruction during the bonding process, which is convenient for ultraviolet lamp irradiation and wiping of overflowed glue with a cotton swab; after the lens is bonded, it can be inspected, and the error caused by one assembly can be modified on the relevant tooling parts to further improve the position accuracy and stability of the bonding.

[0063] In the description of the present invention, it should be understood that the terms "center", "longitudinal", "lateral", "up", "down", "front", "back", "left", "right", "vertical", "horizontal", "top", "bottom", "inside", "outside" and the like indicate orientations or positional relationships based on the orientations or positional relationships shown in the accompanying drawings, and are only for the convenience of describing the present invention and simplifying the description, and do not indicate or imply that the device or element referred to must have a specific orientation, be constructed and operated in a specific orientation, and therefore cannot be understood as limiting the present invention. In addition, the terms "first", "second", etc. are only used for descriptive purposes and cannot be understood as indicating or implying relative importance or implicitly indicating the number of the indicated technical features. Therefore, features defined as "first", "second", etc. may explicitly or implicitly include one or more of the features. In the description of the present invention, unless otherwise specified, "multiple" means two or more.

[0064] In the description of this utility model, it should be noted that, unless otherwise expressly specified or limited, the terms "installed," "connected," and "connected" should be understood in a broad sense. For example, they can refer to fixed connections, detachable connections, or integral connections; mechanical connections, electrical connections; direct connections, indirect connections through an intermediate medium, and internal communication between two components. Those skilled in the art will understand the specific meanings of the above terms in this utility model based on specific circumstances.

[0065] The above description is based on the ideal embodiment of the present invention. Based on the above description, relevant personnel can make various changes and modifications without departing from the technical scope of the present invention. The technical scope of the present invention is not limited to the content of the specification, but must be determined according to the scope of the claims.

Claims

1. A flow cell lens bonding device, characterized in that: The invention comprises a tooling base plate (1) and a fluid chamber assembly component, wherein a detachable positioning seat (2) is mounted on the tooling base plate (1), the positioning seat (2) comprises a seat body (201) connected to the tooling base plate (1), a positioning hole (202) matching the outer diameter of the aspheric lens (7) is provided on the top surface of the seat body (201), and a positioning component for fixing the fluid chamber assembly component is provided at one end of the positioning seat (2); The fluid chamber assembly comprises a fluid chamber body (9), a flow cell body (5) is mounted on the top of the fluid chamber body (9), and a concave reflector (6) is mounted on one side of the flow cell body (5); A baffle (11) is connected to the side of the fluid chamber body (9), and the baffle (11) is perpendicular to the top surface of the fluid chamber body (9). When the baffle (11) and the fluid chamber body (9) are both attached to the outside of the base (201), the concave reflector (6) is coaxial with the positioning hole (202).

2. The flow cell lens bonding device according to claim 1, characterized in that: The positioning assembly comprises a support plate (3) parallel to the baffle (11), the support plate (3) is located at one end of the positioning seat (2), and a lateral adjustment positioning bead (4) for fixing the baffle (11) is passed through the support plate (3).

3. The flow cell lens bonding device according to claim 2, characterized in that: The support plate (3) is provided with a mounting hole (301), and the lateral adjustment positioning bead (4) is screwed and passed through the mounting hole (301).

4. The flow cell lens bonding device according to claim 3, characterized in that: The mounting holes (301) are threaded holes, and at least two mounting holes (301) are provided.

5. The flow cell lens bonding device according to claim 1, characterized in that: A pressure plate (12) is fixedly mounted on the inner side of the baffle (11), and a waste liquid connector (13) is passed through the pressure plate (12); two positioning bosses (8) are relatively arranged on the top of the fluid chamber body (9), one end of the flow cell body (5) is embedded between the two positioning bosses (8), and the other end is in contact with the waste liquid connector (13).

6. The flow cell lens bonding device according to claim 1, characterized in that: Opposite through slots (203) are provided on both sides of the positioning hole (202).

7. The flow cell lens bonding device according to claim 1, characterized in that: An inner hole (204) is provided at the bottom end of the positioning hole (202), and the inner hole (204) is coaxial with the positioning hole (202).

8. The flow cell lens bonding device according to claim 1, characterized in that: A connecting hole (205) is provided on the seat body (201), and one end of a fastener passes through the connecting hole (205) and is screwed into the tooling base plate (1).

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