Cell strain concentration device

Abstract

The utility model discloses a cell strain concentration device, which belongs to the field of solution processing equipment and is characterized in that a hollow tubular membrane component is in threaded connection in a storage tank along the vertical direction, the storage tank is divided into an inner strain tank and an outer clean water tank, and an online conductivity meter monitors the salinity concentration of a strain solution in the strain tank in real time; the addition amount of clear water is adjusted, and the motor drives the stirring device to stir a strain stock solution in the strain pool, so that the clear water in the strain stock solution penetrates through the pore diameter of the microporous filter membrane layer of the hollow tube type membrane assembly and flows into the clear water pool through the opening formed in the outer wall of the shell, and strains are difficult to penetrate through the microporous filter membrane layer and are retained in the strain pool; therefore, the concentration of the strain stock solution is realized.

Description

Technical Field

[0001] The utility model belongs to the field of solution processing equipment, and in particular relates to a cell strain concentration device. Background Art

[0002] Bacterial concentration involves treating a liquid containing bacterial colonies to reduce its volume, concentrating the bacteria and increasing the number of bacteria per unit volume. This concentrated bacterial solution can be better used for bacterial culture, identification, and virology research. Furthermore, concentrated bacterial solutions make it easier to extract proteins and other intracellular substances, improving their purity and yield.

[0003] During the bacterial strain concentration process, high water content can affect the concentration effect, thereby reducing the quality of the bacterial strain concentrate. Low water content can significantly increase the salinity of the concentrate, which can easily lead to the death of cells and bacteria when the salinity fluctuates dramatically. Therefore, to meet actual production needs, a cell and bacterial strain concentration device is urgently needed that can ensure the yield of cells and bacteria while preventing cell and bacterial mortality during the concentration process. Utility Model Content

[0004] The purpose of the utility model is to provide a cell strain concentration device to achieve a rapid concentration operation on the bacterial solution, ensure the concentration effect, and thus improve the production quality of the bacterial concentrate.

[0005] In order to solve the above technical problems, the present invention adopts the following technical solutions:

[0006] A cell culture concentration device includes a storage tank, in which a hollow tubular membrane assembly is provided. The hollow tubular membrane assembly is threadedly connected to the storage tank in a vertical direction, dividing the storage tank into an inner culture tank and an outer clear water tank. An online conductivity meter, an electric air pressure pump and a stirring device driven by a motor are electrically connected above the culture tank, and the measuring plug of the online conductivity meter passes through the interior of the culture tank.

[0007] During use, the hollow tubular membrane assembly is threaded into a reservoir, dividing the reservoir into an inner bacterial seed pool and an outer clear water pool. The bacterial seed solution to be concentrated is injected into the pool, and the clear water in the solution passes through the hollow tubular membrane assembly into the clear water pool, thereby concentrating the bacterial seed solution within the pool. An online conductivity meter is installed above the pool, and its measuring plug, inserted through the pool, monitors the salt concentration of the solution in real time.

[0008] Preferably, the hollow tubular membrane assembly comprises a shell, a plurality of rectangular openings are provided on the outer wall of the shell, a detachable microporous filter membrane layer is provided on the inner surface of the shell, and a thread and a connecting seat are provided at the lower end of the shell.

[0009] During use, this optimized solution connects the hollow tubular membrane assembly to the center of the reservoir via threads, with a connector ensuring a secure connection. The inner surface of the hollow tubular membrane assembly's housing is equipped with a removable microporous membrane layer, allowing for regular replacement of the membrane. During the concentration process, clear water from the bacterial stock solution passes through the pores of the microporous membrane layer and flows into the clear water reservoir through openings in the housing's outer wall. This prevents bacterial components from penetrating the microporous membrane layer, retaining the majority of the bacterial strain within the reservoir, thus achieving concentration.

[0010] Preferably, the stirring device includes a stirring shaft and stirring paddles, the stirring shaft extending into the culture pool, and the stirring shaft is fixedly connected to stirring paddles distributed circumferentially. A water inlet pipe and a raw liquid inlet pipe are also provided above the culture pool, and the lower ends of the water inlet pipe and the raw liquid inlet pipe extend into the culture pool.

[0011] When this optimized solution is in use, if the salt concentration in the culture pool measured by the online conductivity meter exceeds the set standard, clean water is injected into the pool through the water inlet pipe. This prevents the concentrate from becoming too low in water, which could cause a significant increase in salt content and potentially kill the bacteria. If the salt concentration in the culture pool measured by the online conductivity meter falls below the set standard, the water inlet pipe stops injecting clean water, ensuring the highest cell and bacterial yield. A stirring device is installed to agitate the bacterial solution in the culture pool, ensuring the uniformity of the stock solution and the accuracy of the online conductivity meter's measurement results.

[0012] The clean water and bacterial strain stock solution can be added into the bacterial strain pool manually through the water inlet pipe and the stock solution inlet pipe respectively, or a clean water adding device and a stock solution adding device connected to the online conductivity meter circuit can be provided to electrically control the clean water adding device and the stock solution adding device to automatically add clean water and bacterial strain stock solution through the water inlet pipe and the stock solution inlet pipe.

[0013] Preferably, the electric air pressure pump is connected to the interior of the culture pool through an exhaust pipe.

[0014] When this optimized solution is in use, the electric air pressure pump pressurizes the gas in the culture pool through the exhaust pipe, which can accelerate the outflow rate of clean water and speed up the concentration speed.

[0015] Preferably, a clean water discharge pipe communicating with the interior of the clean water tank and a concentrated bacteria liquid discharge pipe communicating with the interior of the bacteria tank are respectively provided on both sides of the bottom end of the storage tank.

[0016] When this optimized solution is in use, the clean water in the original culture solution is filtered through the hollow tubular membrane assembly and enters the clean water pool, and is then immediately discharged through the clean water discharge pipe. The concentrated culture solution in the culture pool is discharged through the concentrated culture solution discharge pipe for collection, and the operation is simple.

[0017] Preferably, the microporous filter layer is composed of a single or multiple layers of microporous filter membrane made of an ultrahigh molecular weight polymer, and the average pore size of the microporous filter membrane is 0.001 μm to 0.008 μm. When the online conductivity meter measures a salt concentration in the culture pool of ≥0.6%, clean water is injected through the water inlet pipe. When the online conductivity meter measures a salt concentration in the culture pool of <0.4%, the injection of clean water through the water inlet pipe is stopped.

[0018] By adopting the above technical solution, the beneficial effects of the utility model are:

[0019] 1. The lower end of the hollow tubular membrane module is threadedly connected to the bottom of the reservoir, making it easy to disassemble and facilitate regular replacement of the microporous filter membrane in the hollow tubular membrane module. An online conductivity meter installed above the culture tank monitors the conductivity of the liquid in the culture tank in real time, converting it into salt concentration. This allows the amount of clean water added to adjust the salt content of the culture stock solution to fluctuate within a certain range during the concentration process, preventing excessive salt concentration in the concentrate, which could cause cell and bacterial death, and ensuring a high yield of the culture concentrate.

[0020] 2. The average pore size of the microporous filter membrane in the hollow tubular membrane assembly is 0.001μm~0.008μm, so that clean water can pass through the microporous filter membrane layer into the clean water pool, and the bacteria cannot pass through the microporous filter membrane layer and continue to remain in the bacteria pool, thereby achieving bacteria concentration. The electric air pressure pump pressurizes the gas in the bacteria pool through the exhaust pipe, which can accelerate the outflow rate of clean water and speed up the concentration speed.

[0021] 3. The setting of the stirring device can stir the bacterial liquid in the bacterial culture pool to ensure the uniformity of the bacterial culture stock solution and the accuracy of the measurement results of the online conductivity meter. The clean water that flows into the clean water pool through the microporous filter layer is immediately discharged through the clean water discharge pipe, and the bacterial concentrate is discharged and collected through the concentrated bacterial liquid discharge pipe. The separation operation is simple and convenient. BRIEF DESCRIPTION OF THE DRAWINGS

[0022] Figure 1 It is a structural schematic diagram of the present invention.

[0023] Figure 2 Schematic diagram of a hollow tubular membrane module.

[0024] Figure 3 This is an enlarged view of the lower end of the hollow tubular membrane module.

[0025] Notes in the figure: 1. Storage tank; 2. Hollow tubular membrane assembly; 3. Bacteria seed tank; 4. Clean water tank; 5. Online conductivity meter; 6. Electric air pump; 7. Measuring plug; 8. Motor; 9. Housing; 10. Microporous filter membrane layer; 11. Opening; 12. Connecting seat; 13. Thread; 14. Stirring shaft; 15. Stirring paddle; 16. Water inlet pipe; 17. Raw liquid inlet pipe; 18. Exhaust pipe; 19. Clean water discharge pipe; 20. Concentrated bacteria liquid discharge pipe. DETAILED DESCRIPTION

[0026] The technical solutions in the embodiments of the utility model will be apparently and completely described in connection with the drawings in the embodiments of the utility model. Obviously, the described embodiments are only part of the embodiments of the utility model, rather than all the embodiments. Based on the embodiments in the utility model, all the other embodiments obtained by the ordinary skilled in the art without creative labor fall within the protection scope of the utility model.

[0027] Please refer to Figure 1-3 The utility model provides a kind of technical solutions: a cell strain concentration device, including reservoir 1, hollow tubular membrane module 2 is equipped in the reservoir 1, the hollow tubular membrane module 2 is connected in reservoir 1 along vertical direction by screw thread, and reservoir 1 is divided into inner layer strain pool 3 and outer layer clean water pool 4, on-line conductivity meter 5, electric air pump 6 and stirring device driven by motor 8 are electrically connected above strain pool 3, and the measuring plug 7 of on-line conductivity meter 5 penetrates strain pool 3 inside.

[0028] The hollow tubular membrane module 2 includes shell 9, a plurality of rectangular openings 11 are equipped on the outer wall of shell 9, detachable microporous filter membrane layer 10 is equipped on the inner surface of shell 9, and threaded 13 and connecting seat 12 are equipped at the lower end of shell 9.

[0029] The hollow tubular membrane module 2 is connected in the middle part of reservoir 1 by threaded 13, is convenient to disassemble, and the setting of connecting seat 12 guarantees the stability of connection. The inner surface of shell 9 in hollow tubular membrane module 2 is equipped with detachable microporous filter membrane layer 10, which is convenient to replace regularly, the microporous filter membrane layer 10 is made of single-layer or multi-layer microporous filter membrane of ultrahigh molecular polymer, and the average pore size of the microporous filter membrane is 0.001 μm-0.008 μm. In the concentration process, the clean water in strain stock solution can penetrate the pore size of microporous filter membrane layer 10, and flow into clean water pool 4 through the opening 11 arranged on the outer wall of shell 9, and the strain components are difficult to penetrate the microporous filter membrane layer 10, and most of the strains are retained in strain pool 3, so as to realize concentration.

[0030] The measuring plug 7 of the online conductivity meter 5 penetrates into the inside of the seed tank 3, which can monitor the conductivity in the seed tank in real time, so as to convert the salt concentration therein. A stirring device is arranged at the middle position above the seed tank 3, which comprises a stirring shaft 14 and a stirring paddle 15. The stirring device can stir the bacterial liquid in the seed tank 3, so as to ensure the uniformity of the seed stock and the accuracy of the measurement result of the online conductivity meter 5. When the salt concentration in the seed tank 3 measured by the online conductivity meter 5 is greater than or equal to 0.6%, the water inlet pipe 16 injects clean water. When the salt concentration in the seed tank 3 measured by the online conductivity meter 5 is less than 0.4%, the water inlet pipe 16 stops injecting clean water, so that the salt concentration in the seed stock fluctuates within a specified range, avoids the high salt concentration in the concentrated liquid, causes the death of cells and bacteria, and ensures the high yield of the bacterial concentrate.

[0031] The clean water and the seed stock can be manually added into the seed tank 3 through the water inlet pipe 16 and the stock inlet pipe 17 respectively, or the clean water adding device and the stock adding device connected with the circuit of the online conductivity meter 5 can be arranged, and the clean water adding device and the stock adding device are automatically controlled to add clean water and seed stock through the water inlet pipe 16 and the stock inlet pipe 17.

[0032] The electric air pressure pump 6 is connected with the inside of the seed tank 3 through the exhaust pipe 18. The electric air pressure pump 6 pressurizes the gas in the seed tank 3 through the exhaust pipe 18, accelerates the penetration rate of the clean water, and speeds up the concentration speed. The bottom end of the storage tank 1 is respectively provided with a clean water discharge pipe 19 connected with the inside of the clean water tank 4 and a concentrated bacterial liquid discharge pipe 20 connected with the inside of the seed tank 3. The clean water flowing into the clean water tank 4 through the microporous filter membrane layer 10 is immediately discharged through the clean water discharge pipe 19, and the bacterial concentrate is discharged and collected through the concentrated bacterial liquid discharge pipe 20, so that the separation operation is simple and convenient.

[0033] Working principle: When the utility model is in use, the hollow tubular membrane assembly is connected to the storage tank along the vertical thread, and the storage tank is divided into the inner bacterial seed tank and the outer clean water tank. The bacterial seed stock solution is added through the stock liquid inlet pipe, the online conductivity meter, the electric air pressure pump and the motor are turned on, and the plug of the online conductivity meter is inserted into the bacterial seed stock solution. The salt concentration of the solution is monitored in real time, and the amount of clean water added is adjusted. The electric air pressure pump injects air pressure into the bacterial seed tank through the exhaust pipe, and the motor drives the stirring device to stir the bacterial seed stock solution in the bacterial seed tank. The clean water in the bacterial seed stock solution passes through the hollow tubular membrane. The pore size of the component passes through the microporous filter membrane layer and flows into the clean water pool through the opening set on the outer wall of the shell. The bacterial strains are difficult to pass through the microporous filter membrane layer and are retained in the bacterial strain pool, thereby achieving the concentration of the bacterial strain stock solution. The clean water in the clean water pool is immediately discharged through the clean water discharge pipe, and the solution in the bacterial strain pool is discharged through the concentrated bacterial liquid discharge pipe to collect the bacterial strain concentrated liquid. The salt concentration of the collected concentrated liquid is between 0.6% and 0.4%, which not only avoids the problem of excessive salt concentration in the concentrated liquid causing cell and bacterial strain death, but also ensures a high yield of the bacterial strain concentrated liquid.

[0034] Of course, the above description is not limited to the above examples. The technical features not described in the present invention can be achieved through or by adopting existing technologies, and will not be repeated here. The above embodiments and drawings are only used to illustrate the technical solution of the present invention and are not limitations of the present invention. The present invention is described in detail with reference to the preferred implementation methods. Ordinary technicians in this field should understand that the changes, modifications, additions or substitutions made by ordinary technicians in this technical field within the essential scope of the present invention do not depart from the purpose of the present invention and should also fall within the scope of protection of the claims of the present invention.

Claims

1. A cell strain concentration device, comprising a storage tank (1), characterized in that: A hollow tubular membrane assembly (2) is provided in the storage tank (1), and the hollow tubular membrane assembly (2) is threadedly connected to the storage tank (1) in a vertical direction, dividing the storage tank (1) into an inner bacterial culture pool (3) and an outer clear water pool (4). An online conductivity meter (5), an electric air pressure pump (6) and a stirring device driven by a motor (8) are electrically connected above the bacterial culture pool (3), and a measuring plug (7) of the online conductivity meter (5) passes through the interior of the bacterial culture pool (3).

2. The cell strain concentration device according to claim 1, characterized in that: The hollow tubular membrane assembly (2) comprises a shell (9), a plurality of rectangular openings (11) are provided on the outer wall of the shell (9), a detachable microporous filter membrane layer (10) is provided on the inner surface of the shell (9), and a thread (13) and a connecting seat (12) are provided at the lower end of the shell (9).

3. The cell strain concentration device according to claim 1, characterized in that: The stirring device comprises a stirring shaft (14) and stirring paddles (15); the stirring shaft (14) extends into the interior of the bacterial culture pool (3); and the stirring shaft (14) is fixedly connected with stirring paddles (15) distributed circumferentially.

4. The cell strain concentration device according to claim 1, characterized in that: A water inlet pipe (16) and a raw liquid inlet pipe (17) are further provided above the bacterial seed pool (3), and the lower ends of the water inlet pipe (16) and the raw liquid inlet pipe (17) extend into the interior of the bacterial seed pool (3).

5. The cell strain concentration device according to claim 1, characterized in that: The electric air pressure pump (6) is connected to the interior of the bacterial culture pool (3) through an exhaust pipe (18).

6. The cell strain concentration device according to claim 1, characterized in that: A clean water discharge pipe (19) communicating with the interior of the clean water tank (4) and a concentrated bacterial liquid discharge pipe (20) communicating with the interior of the bacterial culture tank (3) are respectively provided on both sides of the bottom end of the storage tank (1).

7. The cell strain concentration device according to claim 2, characterized in that: The microporous filter membrane layer (10) is composed of a single layer or multiple layers of microporous filter membrane made of ultrahigh molecular polymer, and the average pore size of the microporous filter membrane is 0.001µm~0.008µm.

8. The cell strain concentration device according to claim 1, characterized in that: When the salt concentration in the bacterial culture pool (3) measured by the online conductivity meter (5) is ≥0.6%, clean water is injected from the water inlet pipe (16); when the salt concentration in the bacterial culture pool (3) measured by the online conductivity meter (5) is <0.4%, the injection of clean water from the water inlet pipe (16) is stopped.

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