Twelve-row reagent card for matching 96-pore plate solid-phase extraction device

Abstract

The utility model provides a 12-row reagent card used for being matched with a 96-pore plate solid-phase extraction device. The 12-row reagent card comprises a reagent card body, a microporous filtering membrane, a sealing cover, a supporting seat and a base, the microporous filtering membrane is arranged at the bottom of the reagent card body, an opening of the reagent card body is sealed by a sealing cover, and the reagent card body is mounted on the base through a supporting seat. Liquid-liquid extraction, the 96-pore plate and solid-phase extraction are effectively combined, the flux is high, liquid in 96 sample pores can be simultaneously filtered at one time, the problems of low filtering efficiency and high cost in the prior art are fundamentally solved, the operations of repeated suction, centrifugation, pipetting and the like in the traditional method are avoided, and the experimental steps are further simplified.

Description

Technical Field

[0001] The utility model belongs to the field of solid phase extraction, in particular to a 12-row reagent card for matching a 96-well plate solid phase extraction device. Background Art

[0002] The microporous filter membrane is made of polymer chemical materials and pore-forming additives that are specially treated and then coated on a support layer. The 96-microporous filter plate is designed for high-throughput sample processing. The filter plate is made of precision injection-molded polystyrene and is compatible with a variety of filter membranes of different types and materials, such as polyvinylidene fluoride (PVDF), mixed cellulose (MCE), polytetrafluoroethylene (PTFE) and other microporous filter membranes. Each plate hole is individually sealed and combined with removable guide plates and collection plates to achieve seamless integration. The main usage methods are filtration and adsorption. Filtration is to intercept bacteria, suspended matter and large molecular impurities outside and filter them to achieve the purpose. Adsorption is because some materials of filter membranes have high protein binding capacity and strong adsorption force, which can adsorb target proteins and enzymes on the membrane, while impurities are retained outside, and water and solvents penetrate through to achieve adsorption, such as nitrocellulose filter membrane (NC). As a new type of high-efficiency separation, concentration, purification and purification technology, microporous membrane filtration technology has been widely used in many fields such as petrochemical industry, light industry and textile, food, medicine, environmental protection, etc. in the past 30 years.

[0003] Domestic medical institutions use microporous membranes with different pore sizes to separate proteins in clinical biochemical tests, which can selectively intercept proteins of various molecular weights in serum or body fluids. This method is simple, requires little dosage, and is low-cost. It is a convenient method for separating proteins in clinical tests and scientific research. It can increase the detection rate of tuberculosis and Shigella dysenteriae in microbiological tests. In immunological tests, HBcAg can be detected using the nitrocellulose membrane-ELISA method. When testing the microbial limits (counts of bacteria, molds, and yeasts) of drugs containing antibacterial ingredients, the membrane filtration method was compared with the elution method. The results showed that the elution method could not accurately reflect the bacterial contamination of the test sample, with an average recovery rate of only 13%. The membrane filtration method can quantitatively reflect the bacterial contamination of the test sample, with an average recovery rate of 97%. It is also easy to operate and requires fewer steps.

[0004] However, the traditional solid phase extraction devices currently used require repeated operations such as aspiration, centrifugation and pipetting, resulting in low filtration efficiency. Summary of the Invention

[0005] In view of this, the present invention aims to overcome the defects in the prior art and proposes a 12-row reagent card for matching a 96-well plate solid phase extraction device.

[0006] In order to achieve the above-mentioned purpose, the technical solution of the utility model is achieved as follows:

[0007] A 12-row reagent card for matching a 96-well plate solid-phase extraction device comprises a reagent card body, a microporous filter membrane, a sealing cover, a support seat and a base; the microporous filter membrane is arranged at the bottom of the reagent card body, the opening of the reagent card body is sealed by the sealing cover, and the reagent card body is installed on the base via the support seat.

[0008] Furthermore, the reagent card body includes a plurality of sample tubes arranged in a row, each having a tapered lower end. The microporous filter membrane is disposed at the bottom of the sample tubes. During installation, the microporous filter membrane is tightly secured to the sample tubes. Positive or negative pressure is applied to cause the liquid in the sample tubes to flow through the microporous filter membrane, thereby filtering the reaction liquid.

[0009] Furthermore, the support base includes a plurality of slots arranged in a row, the upper portion of each slot being adapted to fit the bottom of the sample tube, and the lower portion being adapted to fit the base. A liquid passage hole is provided at the center of each slot, connecting the sample tube to the positive pressure filtration device or the negative pressure filtration device.

[0010] Furthermore, the base includes an upper cover plate and a lower cover plate, a sealing interlayer is provided between the upper cover plate and the lower cover plate, and a filter hole is provided on the base that passes through the upper cover plate, the sealing interlayer and the lower cover plate in sequence, and the filter hole is used to insert the support seat, and the filter hole is adapted to the support seat.

[0011] Furthermore, a platform is provided in the middle of the card slot, and the liquid hole is provided at the center of the platform.

[0012] Furthermore, the upper surface of the platform is provided with a plurality of strip-shaped arc-shaped ridges.

[0013] Furthermore, the sealing cover includes a mounting plate and a raised seal arranged on the lower surface of the mounting plate, and the raised seal is adapted to fit the upper opening of the sample tube.

[0014] Furthermore, the microporous filtration membrane is made of hydrophilic polyethylene.

[0015] Furthermore, the microporous filtration membrane has a pore size of 22 μm and a thickness of 2 mm.

[0016] Compared with the prior art, the present invention has the following advantages:

[0017] (1) The 12-row reagent card for matching a 96-well plate solid phase extraction device of the present invention belongs to the field of sample pretreatment. The 12-row reagent card effectively combines liquid-liquid extraction, 96-well plate and solid phase extraction, has high throughput, and can filter 96 sample wells at one time, fundamentally solving the problems of low filtration efficiency and high cost in the prior art, avoiding repeated aspiration, centrifugation and pipetting operations in traditional methods, and thus simplifying the experimental steps.

[0018] (2) The bottom of the sample tube is respectively packaged with a microporous filter film, the stability between the hole / plate is good, there is no mutual interference, the extraction effect is good, there is no dead cavity volume, the recovery rate is high, the flux is high, 8 reagent cards can be placed at one time to filter 96 samples at the same time, the overall design process is simple, the positive pressure filtering device or the negative pressure suction filtering device is used in combination, the flow rates of the positive pressure filtering and the negative pressure suction filtering can be ensured to be uniform, the operation is simple, no other special instrument equipment is needed, the practicality is high, and the 12-union reagent card is suitable for wide use.

[0019] (3) The 12-union reagent card can be used for 96-hole plate solid-phase extraction, is suitable for high-throughput plasmid / genome extraction, protein purification, new drug screening, receptor action analysis, protein binding determination, protein kinase / phosphatase analysis, ELISPOT analysis, high-throughput analysis, filtration purification, fluorescent dye removal and sample extraction preparation and the like, has diversified application scenes, and ensures that the filtering process is low in amount and free of cross infection.

[0020] (4) The main body material of the 12-union reagent card is selected from medical-grade plastic, is resistant to acid and alkali corrosion, and is firm and durable. BRIEF DESCRIPTION OF DRAWINGS

[0021] The accompanying drawings, which form a part of this description, are included to provide a further understanding of the application and are incorporated in and constitute a part of this application. The embodiments of the application illustrated in the drawings are presented to explain the present application and not intended to limit or restrict the present application in any manner. In the drawings:

[0022] Figure 1 The exploded view of the 12-union reagent card is shown in the drawings;

[0023] Figure 2 The sectional view of the reagent card body, the sealing cover and the support seat after assembly is shown in the drawings;

[0024] Figure 3 The partial sectional view of the support seat is shown in the drawings;

[0025] Figure 4 The top view of the support seat is shown in the drawings;

[0026] Figure 5 The sectional view of the base is shown in the drawings.

[0027] BRIEF DESCRIPTION OF DRAWINGS

[0028] 1, reagent card body; 2, microporous filter film; 3, sealing cover; 31, protruding plugging; 4, base; 11, upper cover plate; 12, sealing interlayer; 13, lower cover plate; 5, support seat; 51, card slot; 52, liquid passage hole; 53, platform; 54, arc-shaped convex rib. DETAILED DESCRIPTION

[0029] It should be noted that the embodiments in the present application and the features in the embodiments can be combined with each other without conflict.

[0030] In the description of the present application, it should be understood that the orientation or positional relationship indicated by the terms "center", "longitudinal", "lateral", "upper", "lower", "front", "rear", "left", "right", "vertical", "horizontal", "top", "bottom", "inner", "outer" and the like is based on the orientation or positional relationship shown in the drawings, and is only for the convenience of describing the present application and simplifying the description, and therefore cannot be understood as indicating or implying that the device or element indicated must have a particular orientation, be constructed and operated in a particular orientation, and therefore cannot be understood as a limitation on the present application. In addition, the terms "first", "second" and the like are only for the purpose of description, and cannot be understood as indicating or implying relative importance or implicitly indicating the number of the technical features indicated. Therefore, the features limited by "first", "second" and the like can explicitly or implicitly include one or more of the features. In the description of the present application, unless otherwise specified, the meaning of "a plurality of" is two or more.

[0031] In the description of the present application, it should be noted that, unless otherwise specified and limited, the terms "mounting", "connection", "connection" should be understood broadly, for example, it can be fixedly connected, or it can be detachably connected, or integrally connected; it can be mechanically connected, or it can be electrically connected; it can be directly connected, or it can be indirectly connected through an intermediate medium, or it can be the communication between two elements. For ordinary skilled in the art, the specific meaning of the above terms in the present application can be understood according to the specific circumstances.

[0032] The present application will be described in detail below with reference to the drawings and in combination with the embodiments.

[0033] Embodiment 1

[0034] Referring to Figures 1-5 The present embodiment provides a 12-row reagent card for matching a 96-well plate solid phase extraction device, which comprises a reagent card body 1, a microporous filter membrane 2, a sealing cover 3, a support seat 5 and a base 4. The microporous filter membrane 2 is arranged at the bottom of the reagent card body 1, the opening of the reagent card body 1 is sealed by the sealing cover 3, and the reagent card body 1 is installed on the base 4 through the support seat 5.

[0035] Among them, referring to Figure 1 and Figure 2The reagent card body 1 comprises a plurality of sample tubes arranged in a row, the lower end of the sample tube is in a tapered shape, and a microporous filter membrane 2 is arranged at the bottom of the sample tube.

[0036] When installed, the microporous filter membrane 2 is tightly fixed on the sample tube. Through the action of positive pressure at the upper end or negative pressure at the lower end, the liquid in the sample tube flows out through the microporous filter membrane 2, and the filtration of the reaction liquid is completed.

[0037] Referring to Figure 1 The sealing cover 3 comprises a mounting plate and a protruding plugging 31 arranged on the lower surface of the mounting plate, the protruding plugging 31 is matched with the upper end opening of the sample tube, and is used for sealing the upper end of the sample tube.

[0038] Referring to Figure 2 , Figure 3 , Figure 5 The support seat 5 comprises a plurality of clamping grooves 51 arranged in a row, the upper part of the clamping groove 51 is matched with the bottom of the sample tube, the lower part is matched with the base 4, and the center of the clamping groove 51 is provided with a liquid passing hole 52 communicating the sample tube and the filter device. The platform 53 is arranged in the middle part of the clamping groove 51, and the liquid passing hole 52 is arranged at the center of the platform 53.

[0039] Referring to Figure 5 The base 4 comprises an upper cover plate 11 and a lower cover plate 13, and a sealing interlayer 12 is arranged between the upper cover plate 11 and the lower cover plate 13. The filter hole is arranged on the base 4 and penetrates the upper cover plate 11, the sealing interlayer 12 and the lower cover plate 13 in sequence, the filter hole is used for inserting the support seat 5, and the filter hole is matched with the support seat 5.

[0040] In use, the positive pressure filter device / negative pressure filter device is started, the sample liquid is poured into the sample tube, and the reagent card is inserted into the support seat 5, and the support seat 5 is inserted into the filter hole of the base 4. When the positive pressure filter device is started, the sample liquid can be discharged through the filter membrane 2 by the action from top to bottom; when the negative pressure filter device is started, the negative pressure filter device can form a negative pressure environment, so that the sample liquid in the sample tube can be filtered through the microporous filter membrane 2 and then discharged. The solid in the sample liquid is intercepted on the microporous filter membrane 2, and is used for further operation.

[0041] When necessary, the protruding plugging 31 on the sealing cover 3 is matched with the filter hole on the base 4, and when the base 4 is not filled, the sealing cover 3 can be used to seal the filter hole on the base 4 which is not used.

[0042] Referring to Figure 1 and Figure 5The base 4 comprises an upper cover plate 11 and a lower cover plate 13, and a sealing interlayer 12 is arranged between the upper cover plate 11 and the lower cover plate 13. The sealing interlayer 12 improves the sealing performance between the upper cover plate 11 and the lower cover plate 13. In addition, the support seat 5 is matched with the filter hole, so that the support seat 5 is tightly combined with the filter hole after being inserted into the filter hole, the sealing performance between the support seat 5 and the hole wall of the filter hole is improved, and the sealing performance between the support seat 5 and the negative pressure filtration device is improved, thereby ensuring that the filtration operation is smoothly and reliably performed, and the technical problems of poor sealing performance and difficult plugging between the negative pressure filtration device and the reagent card in the prior art are solved, and the detection efficiency is improved.

[0043] Preferably, in the embodiment, the sealing interlayer 12 is an elastic member, for example, the material of the sealing interlayer 12 is silica gel, rubber or plastic.

[0044] Preferably, in the embodiment, the number of sample tubes is 12, the number of clamping grooves 51 on the support seat 5 is 12, the number of protruding plugs 31 on the sealing cover 3 is 12, and the number of filter holes on the base 4 is 96. Of course, in order to adapt to other hole numbers of hole plates on the market, such as 6 holes, 12 holes, 24 holes, 48 holes and the like, the number of sample tubes, the clamping grooves 51 on the support seat 5, the protruding plugs 31 on the sealing cover 3 and the filter holes on the base 4 in the utility model can be adjusted adaptively.

[0045] Preferably, in the embodiment, the pore size of the microporous filtration membrane 2 is 22 μm, and the thickness is 2 mm. The pore size and the thickness can be adjusted according to the sample in the sample tube, which is not limited herein.

[0046] Embodiment 2

[0047] Embodiment 2 provides a 12-row reagent card, which comprises the 12-row reagent card of embodiment 1, and further comprises a plurality of strip-shaped arc-shaped ribs 54 arranged on the upper surface of the platform 53 in the clamping groove 51 of the support seat 5. The arc-shaped ribs 54 support the microporous filtration membrane 2 on the sample tube, effectively avoiding the loss caused by the rupture of the microporous filtration membrane 2 due to excessive vacuum pressure during equipment operation.

[0048] Specifically, referring to Figure 3 and Figure 4 , the number of arc-shaped ribs 54 is three, and a gap is left between each arc-shaped rib 54, which can effectively prevent liquid from being retained on the surface of the platform 53 during the filtration process, and further improve the effect of the filtration operation.

[0049] In the embodiment, the technical features of the 12-row reagent card disclosed in embodiment 1 are also applicable to the embodiment, and the technical features of the 12-row reagent card disclosed in embodiment 1 are not described again.

[0050] The 12-row reagent card can be extended to high-throughput plasmid / genome extraction, protein purification, new drug screening, receptor action analysis, protein binding determination, protein kinase / phosphatase analysis, ELISPOT analysis, high-throughput analysis, filtration purification, fluorescent dye removal and sample extraction preparation and other application scenarios, which can shorten the filtration time and avoid cross contamination, further alleviate the technical problems of repeated suction, centrifugation and pipetting operation in the prior art, poor sealing and reduced sample liquid detection efficiency.

[0051] The 12-row reagent card of the embodiment has the advantages of the 12-row reagent card of embodiment 1, which have been described in detail in embodiment 1 and will not be repeated here.

[0052] The above is only a preferred embodiment of the present application, and is not intended to limit the present application. Any modification, equivalent replacement, improvement, etc. made within the spirit and principles of the present application shall be included in the protection scope of the present application.

Claims

1. A 12-strip reagent card for use with a 96-well plate solid phase extraction device, characterized in that: It includes a reagent card body, a microporous filter membrane, a sealing cover, a support seat and a base; the microporous filter membrane is arranged at the bottom of the reagent card body, the opening of the reagent card body is sealed by the sealing cover, and the reagent card body is installed on the base through the support seat.

2. The 12-row reagent card for use with a 96-well plate solid phase extraction apparatus according to claim 1, characterized in that: The reagent card body comprises a plurality of sample tubes arranged in a row, the lower ends of the sample tubes are tapered, and the microporous filter membrane is arranged at the bottom of the sample tubes.

3. The 12-strip reagent card for use with a 96-well plate solid phase extraction apparatus according to claim 1, characterized in that: The support base includes a plurality of slots arranged in a row, the upper portion of the slots is adapted to the bottom of the sample tube, and the lower portion is adapted to the base, and a liquid hole is provided at the center of the slots.

4. The 12-strip reagent card for use with a 96-well plate solid phase extraction apparatus according to claim 1, characterized in that: The base includes an upper cover plate and a lower cover plate, a sealing interlayer is provided between the upper cover plate and the lower cover plate, and a filter hole is provided on the base that passes through the upper cover plate, the sealing interlayer and the lower cover plate in sequence, and the filter hole is used to insert the support seat, and the filter hole is adapted to the support seat.

5. The 12-strip reagent card for use with a 96-well plate solid phase extraction apparatus according to claim 3, characterized in that: A platform is provided in the middle of the card slot, and the liquid hole is provided at the center of the platform.

6. The 12-strip reagent card for use with a 96-well plate solid phase extraction apparatus according to claim 5, characterized in that: The upper surface of the platform is provided with a plurality of strip-shaped arc-shaped ridges.

7. The 12-strip reagent card for use with a 96-well plate solid phase extraction apparatus according to claim 2, characterized in that: The sealing cover includes a mounting plate and a raised seal arranged on the lower surface of the mounting plate, and the raised seal is adapted to the upper end opening of the sample tube.

8. The 12-strip reagent card for use with a 96-well plate solid phase extraction apparatus according to claim 1, characterized in that: The material of the microporous filtration membrane is hydrophilic polyethylene.

9. The 12-strip reagent card for use with a 96-well plate solid phase extraction apparatus according to claim 1, characterized in that: The microporous filtration membrane has a pore size of 22 μm and a thickness of 2 mm.

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