Positioning device for tail puncture of laboratory mice

Abstract

The utility model discloses a positioning device for tail puncture of laboratory mice, which comprises a needle cylinder, and a push rod is arranged in the needle cylinder. The bottom side of the needle cylinder is fixedly connected to the connector; a needle head is detachably connected in the connector; the outer side of the connector is sleeved with a positioning device, and the positioning device comprises a positioning cylinder, an adjusting cylinder and a spreader; the positioning cylinder is fixedly sleeved on the outer side of the connector, and scales are arranged on the outer wall of the positioning cylinder; a gap is reserved between the inner wall of the adjusting cylinder and the outer wall of the needle head, and the bottom end face of the adjusting cylinder is flush with the bottom end face of the needle point. The bottom side of the adjusting cylinder is sleeved with the spreader. Through the cooperation of the positioning cylinder and the adjusting cylinder, the exposed length of the needle head each time can be adjusted, so that when experimenters respectively puncture the tails of a plurality of white rats, the inserted lengths of the needle head are kept consistent; in combination with the use of the dilator, the epidermal tissue wound punctured by the tail of the white mouse can be slightly expanded outwards, so that the irradiation of more light rays is facilitated, and the solidification of a tissue engineering scaffold material is accelerated.

Description

Technical Field

[0001] This utility model relates to the field of tail puncture technology for rodents, and in particular to a positioning device for tail puncture of laboratory rodents. Background Technology

[0002] Among mammalian laboratory animals, white mice are the most widely used and employed in various experimental studies due to their ease of husbandry, management, and control. Some experimental procedures involve puncturing the caudal vertebrae of the white mouse. After fixing the mouse in place, its tail is stretched taut, and alcohol swabs are used to repeatedly wipe the tail to dilate blood vessels and soften the epidermal keratin. The sides of the tail are then held, and the needle is inserted vertically into the intervertebral disc of the caudal vertebrae.

[0003] To ensure the rigor and accuracy of experimental data, multiple mice are often used in experiments to perform tail vertebrae punctures. To guarantee consistent damage to each mouse's tail, the puncture depth must be uniform. However, tail punctures rely heavily on the experimenter's touch, making it difficult to ensure precise and consistent needle insertion depth across all mice, thus affecting the rigor and accuracy of the experiment. Some puncture needles use custom-made needle plugs to control the insertion depth, but these plugs often only allow for a single puncture depth and cannot be adjusted for different experimental needs. Utility Model Content

[0004] This application provides a positioning device for tail puncture in laboratory mice. The length of the needle protruding each time can be adjusted by the cooperation of the positioning tube and the adjusting tube, so that the insertion length of the needle remains consistent when the experimenter performs punctures on the tails of multiple mice. Combined with the use of a spreader, the epidermal tissue wound punctured in the mouse tail can be slightly widened to facilitate more light irradiation and accelerate the coagulation of tissue engineering scaffold materials.

[0005] This application provides a positioning device for tail puncture in laboratory mice, including a syringe, a push rod inside the syringe, a push seat fixedly connected to the bottom side of the push rod, the push seat being attached to but not fixed to the inner wall of the syringe; a connector fixedly connected to the bottom side of the syringe, the connector communicating with the syringe; a needle detachably connected inside the connector; and a positioning device fitted on the outside of the connector.

[0006] The positioning device includes a positioning cylinder, an adjusting cylinder, and a spreader;

[0007] The positioning cylinder is fixedly fitted on the outside of the connector, and its outer wall is marked with graduations; the adjusting cylinder is rotatably connected inside the positioning cylinder, and there is a gap between the inner wall of the adjusting cylinder and the outer wall of the needle, and the bottom end face of the adjusting cylinder is flush with the bottom end face of the needle tip; the spreader is fitted on the outside of the bottom side of the adjusting cylinder.

[0008] Furthermore, the inner wall of the positioning cylinder is provided with an internal thread, and the outer wall of the adjusting cylinder is provided with an external thread. The adjusting cylinder is rotatably connected to the positioning cylinder through the thread.

[0009] Furthermore, the positioning cylinder is made transparent.

[0010] Furthermore, the spreader includes a spreader sleeve and an inner tube;

[0011] The inner tube is detachably fitted onto the outside of the adjusting cylinder. The sleeve is opened and rotated to fit onto the outside of the inner tube, and it is set in a conical shape.

[0012] Furthermore, an inverted conical threaded through hole is opened inside the expanding sleeve, and an external thread is opened on the outer wall of the inner tube. The inner tube is rotatably connected to the expanding sleeve through the thread.

[0013] Furthermore, the outer wall of the expanded sleeve is provided with at least three through grooves, which are evenly distributed around the outer wall of the expanded sleeve.

[0014] Furthermore, an installation block is fixedly connected to the outer wall of the expansion sleeve, an installation rod is fixedly connected to the installation block, an installation collar is fixedly connected to the installation rod, and a flashlight is detachably connected inside the installation collar.

[0015] Furthermore, the mounting rod is made of aluminum-magnesium alloy tubing.

[0016] Furthermore, the mounting collar and the flashlight are installed using an interference fit; similarly, the inner tube is also fitted onto the outside of the adjusting cylinder using an interference fit.

[0017] One or more technical solutions provided in this application embodiment have at least the following technical effects or advantages: The tail of the white mouse is selected as the puncture site. The adjusting cylinder 22 is in its original position. The adjusting cylinder 22 is rotated according to the puncture depth. The upper end of the adjusting cylinder 22 moves upward according to the scale on the positioning cylinder 21, thereby adjusting the length of the needle 13 protruding. After the length of the needle 13 is adjusted, the needle is directly and vertically inserted into the tail of the white mouse for puncture. If ordinary drugs are injected after puncture, the retractor can be removed from the adjusting cylinder 22 before puncture. If tissue engineering scaffold material is injected after puncture, the retractor can be pre-installed in the adjusting cylinder 22 before puncture. After the rat's tail is punctured, the retractor is lowered and inserted into the wound site of the punctured skin tissue on the tube 22. Then, the needle 13 is removed, and the retractor sleeve 24 is inserted into the wound site of the punctured skin tissue on the rat's tail. Then, the inner tube 23 is screwed to open the retractor sleeve 24, thereby slightly widening the wound site of the punctured skin tissue on the rat's tail. The tissue engineering scaffold material is then inserted into the puncture site of the rat. Then, the flashlight 28 is attached to the mounting ring 27, and the beam angle of the flashlight 28 is adjusted by the mounting rod 26 so that the light shines from above the inner tube 23, so that a large amount of light is focused on the wound site of the punctured skin tissue on the rat's tail. The combination of the positioning tube and the adjusting tube allows for adjustment of the needle's protrusion length each time, ensuring that the needle insertion length remains consistent when puncturing the tails of multiple mice, thus guaranteeing the rigor of controlled variables in the experimental procedure. Furthermore, the use of a spreader slightly widens the epidermal tissue wound punctured at the mouse tail, facilitating greater light exposure and accelerating the stable formation of tissue engineering scaffold materials (interventional therapeutic drugs). Attached Figure Description

[0018] Figure 1 This is a schematic diagram of the installation of the positioning device in this application;

[0019] Figure 2 This is a schematic diagram of the disassembled structure of the positioning device and syringe in this application;

[0020] Figure 3 This is a schematic diagram of the adjustment structure of the positioning device in this application;

[0021] Figure 4 Front view of the positioning device of this application;

[0022] Figure 5 This is a schematic diagram of the disassembled structure of the positioning device in this application;

[0023] Figure 6 This is a schematic diagram of the structure of the expander in this application.

[0024] In the diagram: 10 Syringe, 11 Push rod, 12 Connector, 13 Needle, 20 Positioning device, 21 Positioning cylinder, 22 Adjusting cylinder, 23 Inner tube, 24 Spreading sleeve, 25 Mounting block, 26 Mounting rod, 27 Mounting collar, 28 Flashlight, 29 Inverted tapered threaded through hole. Detailed Implementation

[0025] To facilitate understanding of this utility model, a more complete description will be given below with reference to the accompanying drawings. The drawings illustrate preferred embodiments of this utility model. However, this utility model can be implemented in many different forms and is not limited to the embodiments described herein. Rather, these embodiments are provided to provide a more thorough and complete understanding of the disclosure of this utility model.

[0026] It should be noted that when an element is referred to as being "fixed to" another element, it can be directly attached to the other element or there may be an intervening element. When an element is referred to as being "connected to" another element, it can be directly connected to the other element or there may be an intervening element. The terms "vertical," "horizontal," "left," "right," and similar expressions used herein are for illustrative purposes only and do not represent the only possible implementation.

[0027] Unless otherwise defined, all technical and scientific terms used herein have the same meaning as commonly understood by one of ordinary skill in the art to which this invention pertains. The terminology used herein is for the purpose of describing particular embodiments only and is not intended to be limiting of the invention. The term "and / or" as used herein includes any and all combinations of one or more of the associated listed items.

[0028] Example 1

[0029] Please see Figure 1-6 A positioning device for tail puncture in laboratory mice, comprising a syringe 10.

[0030] The syringe 10 contains a push rod 11, and a push seat is fixedly connected to the bottom of the push rod 11. The push seat is attached to the inner wall of the syringe 10 but not fixed, so that the push rod 11 can push and pull the push seat to move up and down inside the syringe 10. The bottom of the syringe 10 is fixedly connected to a connector 12, and the connector 12 communicates with the syringe 10. A needle 13 is detachably connected inside the connector 12. A positioning device 20 is fitted on the outside of the connector 12, which can adjust the length of the needle 13 that is exposed each time, so that when the experimenter punctures the tails of multiple white mice, the insertion length of the needle 13 is consistent.

[0031] The positioning device 20 includes a positioning cylinder 21, an adjusting cylinder 22, and a spreader;

[0032] The positioning cylinder 21 is fixedly fitted on the outside of the connector 12, and its outer wall is provided with a scale; the adjusting cylinder 22 is rotatably connected inside the positioning cylinder 21, and there is a gap between the inner wall of the adjusting cylinder 22 and the outer wall of the needle 13, and the bottom end face of the adjusting cylinder 22 is flush with the bottom end face of the needle tip; the spreader is fitted on the bottom side of the adjusting cylinder 22.

[0033] The inner wall of the positioning cylinder 21 is threaded internally, and the outer wall of the adjusting cylinder 22 is threaded externally. The adjusting cylinder 22 is rotatably connected to the positioning cylinder 21 through the thread.

[0034] The positioning cylinder 21 is made transparent to facilitate the observation of the position and distance of the adjustment cylinder 22 by the experimenter.

[0035] In other words, when performing a tail puncture on a white mouse, the adjusting cylinder 22 is in its original position. The adjusting cylinder 22 is turned according to the depth of the puncture. The upper end of the adjusting cylinder 22 moves upward according to the scale on the positioning cylinder 21, thereby adjusting the length of the needle 13 protruding. After the length of the needle 13 is adjusted, the needle is inserted vertically into the tail of the white mouse for puncture. The shielding at the bottom of the adjusting cylinder 22 ensures that the length of the needle 13 inserted is consistent when puncturing the tails of multiple white mice.

[0036] Since drugs are injected into the puncture site after mouse puncture, experimental studies are conducted through drug injection. However, sometimes the injected drug is a tissue engineering scaffold material. After injection, the drug needs to be irradiated with ultraviolet light to accelerate its coagulation. By setting up a spreader, the epidermal tissue wound punctured in the mouse tail can be slightly enlarged to increase the amount of light irradiated and accelerate the coagulation of the drug.

[0037] Tissue engineering scaffold materials are usually made by mixing ordinary active drugs with tissue engineering scaffold materials that encapsulate drugs. For example, photosensitive cross-linked biocolloids are mixed with ordinary active drugs and solidified under ultraviolet light to enable the active drugs to exert their effects stably and for a long time.

[0038] The spreader includes a spreader sleeve 24 and an inner tube 23;

[0039] The inner tube 23 is detachably fitted onto the outside of the adjusting cylinder 22, and the sleeve 24 is rotated and fitted onto the outside of the inner tube 23, which is set in a conical shape.

[0040] An inverted tapered threaded through hole 29 is opened inside the sleeve 24, and an external thread is opened on the outer wall of the inner tube 23. The inner tube 23 is rotatably connected to the sleeve 24 through the thread.

[0041] The outer wall of the expansion sleeve 24 is provided with at least three through slots, which are evenly distributed around the outer wall of the expansion sleeve 24. When the inner tube 23 rotates, the expansion sleeve 24 can be expanded outward, thereby slightly widening the epidermal tissue wound punctured at the tail of the white rat (the working method between the expansion sleeve 24 and the inner tube 23 is similar to that of existing expansion bolts).

[0042] An installation block 25 is fixedly connected to the outer wall of the sleeve 24. An installation rod 26 is fixedly connected to the installation block 25. An installation collar 27 is fixedly connected to the installation rod 26. A flashlight 28 is detachably connected inside the installation collar 27.

[0043] The mounting rod 26 is made of aluminum-magnesium alloy tubing, which can be bent at any angle to adjust the beam angle of the flashlight 28, so that a large amount of light is focused on the epidermal tissue wound punctured in the tail of the white rat, and at the same time, it is irradiated at close range to accelerate the coagulation of the tissue engineering scaffold material (it should be noted that the epidermal tissue wound punctured in the tail of the white rat is not a needle hole, but an epidermal tissue wound that exposes the tailbone after contusion).

[0044] The mounting ring 27 and the flashlight 28 are installed using an interference fit; that is, after the flashlight 28 is manually inserted into the mounting ring 27, the flashlight 28 can be installed relatively stably in the mounting ring 27, and can be removed manually later.

[0045] Similarly, the inner tube 23 is also fitted onto the outside of the adjusting tube 22 by an interference fit. If ordinary drugs are injected during the experiment, the retractor can be removed from the adjusting tube 22. If tissue engineering scaffold material is injected, the retractor can be installed on the adjusting tube 22. After the rat tail puncture is completed, the retractor is moved down and inserted into the epidermal tissue wound position of the rat tail puncture. Then the needle 13 is removed, and the retractor remains at the epidermal tissue wound position of the rat tail puncture. (It should be noted that when the retractor is installed on the adjusting tube 22, the bottom surface of the retractor sleeve 24 is slightly higher than the bottom surface of the adjusting tube 22 by 3-5mm to avoid obstructing the needle 13 puncture. At the same time, the flashlight 28 is not installed on the mounting collar 27.)

[0046] When injecting drugs, the speed at which the experimenter pushes the plunger 11 or the friction between the syringe 10 and the plunger can be increased to ensure that the drug is injected slowly and to control the amount of drug injected (the outer wall of the plunger is fitted with a rubber ring to increase the friction between the plunger and the syringe 10 and to prevent the plunger from sliding too fast).

[0047] Flashlight 28 can be a small flashlight that emits ultraviolet light.

[0048] In actual operation of this embodiment, the tail of the mouse is selected as the puncture site. The adjusting cylinder 22 is in its original position. The adjusting cylinder 22 is rotated according to the puncture depth. The upper end of the adjusting cylinder 22 moves upward according to the scale on the positioning cylinder 21, thereby adjusting the length of the needle 13 protruding. After the length of the needle 13 is adjusted, the needle is directly and vertically inserted into the tail of the mouse for puncture. If ordinary drugs are injected after puncture, the retractor can be removed from the adjusting cylinder 22 before puncture. If tissue engineering scaffold material is injected after puncture, the retractor is pre-installed on the adjusting cylinder 22 before puncture. After the puncture is completed, the retractor is lowered and inserted into the epidermal tissue wound at the tail of the mouse. Then, the needle 13 is removed, and the retractor sleeve 24 is inserted into the epidermal tissue wound at the tail of the mouse. Then, the inner tube 23 is screwed to open the retractor sleeve 24, thereby slightly widening the epidermal tissue wound at the tail of the mouse. The tissue engineering scaffold material is then inserted into the puncture site of the mouse. Then, the flashlight 28 is attached to the mounting ring 27, and the illumination angle of the flashlight 28 is adjusted by the mounting rod 26 so that the light shines from above the inner tube 23, so that a large amount of light is focused on the epidermal tissue wound at the tail of the mouse.

[0049] The technical solutions described in the above embodiments of this application have at least the following technical effects or advantages: the length of the needle protruding each time can be adjusted by the cooperation of the positioning tube and the adjusting tube, so that when the experimenter performs punctures on the tails of multiple mice, the length of the needle insertion is consistent, ensuring the rigor of the experimental operation control variables; combined with the use of the spreader, the epidermal tissue wound punctured on the tail of the mouse can be slightly expanded to facilitate more light irradiation and accelerate the stable formation of tissue engineering scaffold materials (interventional therapeutic drugs).

[0050] The mounting collar and the flashlight can also be fitted with a clearance fit. A rubber pad is installed inside the mounting collar, which increases the friction between the mounting collar and the flashlight. The rubber pad also has a certain degree of elasticity, so that the flashlight will not fall off after installation and it is easy to disassemble and reassemble later.

[0051] A similar method can be used between the inner tube and the regulating cylinder.

[0052] Since the flashlight has a certain weight, to prevent the spreader from falling off the mouse's tail due to tilting, you can gently hold it with your hand while shining the light.

[0053] The above description is merely a preferred embodiment of this utility model and is not intended to limit the scope of this utility model. For those skilled in the art, various modifications and variations are possible with this utility model. Any modifications, equivalent substitutions, or improvements made within the spirit and principles of this utility model should be included within the protection scope of this utility model.

Claims

1. A positioning device for tail puncture in laboratory mice, comprising a syringe, characterized in that: The syringe contains a push rod, and a push seat is fixedly connected to the bottom side of the push rod. The push seat is attached to the inner wall of the syringe but not fixed. The bottom side of the syringe is fixedly connected to a connector, which communicates with the syringe. A needle is detachably connected inside the connector. A positioning device is fitted on the outside of the connector. The positioning device includes a positioning cylinder, an adjusting cylinder, and a spreader; The positioning cylinder is fixedly fitted on the outside of the connector, and its outer wall is marked with graduations; the adjusting cylinder is rotatably connected inside the positioning cylinder, and there is a gap between the inner wall of the adjusting cylinder and the outer wall of the needle, and the bottom end face of the adjusting cylinder is flush with the bottom end face of the needle tip; the spreader is fitted on the outside of the bottom side of the adjusting cylinder.

2. The positioning device for tail puncture in laboratory mice as described in claim 1, characterized in that: The positioning cylinder has an internal thread on its inner wall, and the adjusting cylinder has an external thread on its outer wall. The adjusting cylinder is rotatably connected to the positioning cylinder through the thread.

3. The positioning device for tail puncture in laboratory mice as described in claim 1, characterized in that: The positioning cylinder is made transparent.

4. The positioning device for tail puncture in laboratory mice as described in claim 1, characterized in that: The spreader includes a spreading sleeve and an inner tube; The inner tube is detachably fitted onto the outside of the adjusting cylinder. The sleeve is opened and rotated to fit onto the outside of the inner tube, and it is set in a conical shape.

5. The positioning device for tail puncture in laboratory mice as described in claim 4, characterized in that: The inner tube has an inverted conical threaded through hole, and the outer wall of the inner tube has an external thread. The inner tube is rotatably connected to the inner tube through the thread.

6. The positioning device for tail puncture in laboratory mice as described in claim 5, characterized in that: The outer wall of the expanding sleeve has at least three through grooves, which are evenly distributed around the outer wall of the expanding sleeve.

7. A positioning device for tail puncture in laboratory mice as described in claim 6, characterized in that: An installation block is fixedly connected to the outer wall of the expansion sleeve, an installation rod is fixedly connected to the installation block, an installation collar is fixedly connected to the installation rod, and a flashlight is detachably connected inside the installation collar.

8. The positioning device for tail puncture in laboratory mice as described in claim 7, characterized in that: The mounting rod is made of aluminum-magnesium alloy tubing.

9. A positioning device for tail puncture in laboratory mice as described in claim 7, characterized in that: The mounting collar and the flashlight are installed using an interference fit; similarly, the inner tube is also fitted onto the outside of the adjusting cylinder using an interference fit.

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