Glue making instrument
By designing the liquid addition component and swing arm component of the gel casting apparatus, the problems of gel clogging and complex operation in traditional gel casting methods have been solved, achieving stable and uniform production of concentration gradient gels and simplifying the operation process.
Patent Information
- Application Number
- CN202422795202.1
- Authority / Receiving Office
- CN · China
- Patent Type
- Utility models(China)
- Current Assignee / Owner
- Filing Date
- 2024-11-17
- Publication Date
- 2025-10-28
- Estimated Expiration
- 2034-11-17
AI Technical Summary
Traditional gel preparation methods have problems such as the risk of gel clogging the pipeline and complicated operation, making it difficult to produce concentration gradient gels, and existing equipment cannot achieve a stable and uniform gel preparation process.
The gel casting apparatus, which includes a liquid dispensing component and a swing arm component, achieves precise control and mixing of reagents through five pumps. Combined with the height adjustment and position recognition mechanism of the swing arm component, it ensures uniform delivery of reagents and stability of gel.
This method enables the stable production of concentration gradient gels, avoids the risk of gel clogging, simplifies the operation process, and improves the stability and precision of gel production.
Smart Images
Figure CN223474983U_ABST
Abstract
Description
Technical Field
[0001] This application relates to the field of biotechnology, and in particular to a gel-making apparatus. Background Technology
[0002] Western blotting uses vertical electrophoresis gels. Traditionally, various components are manually mixed to prepare uniformly concentrated, layered SDS-PAGE gels. To create gradient gels with increasing concentrations from top to bottom, a twin-cylinder mixer based on the principle of communicating vessels is required. This involves connecting the bottoms of two cylinders containing reagents of different concentrations. The lower concentration reagent is poured into the designated container at a set rate. As the lower concentration reagent decreases, the higher concentration reagent flows into the lower concentration cylinder, gradually increasing the concentration of subsequent reagents until all reagents are in the designated container. The later-added higher concentration reagent pushes the earlier-added lower concentration liquid to the top, forming a concentration gradient from top to bottom. Traditional gel preparation methods can only produce uniform, fixed-concentration gels, while twin-cylinder mixers can produce gradient gels. However, twin-cylinder mixers require pre-mixing a gel accelerator in the preparation reagents, posing a risk of gel clogging during preparation. The operation is also more complex, requiring all reagents to be used at once, making it inconvenient. Utility Model Content
[0003] Purpose of the utility model: This application provides a glue-making device. The swing arm assembly of the glue-making device can be adjusted in height according to different situations, and the operating mechanism of the glue-making device can ensure the stability of glue making.
[0004] Technical Solution: This utility model provides a glue-making device, which includes: a liquid-adding assembly, the liquid-adding assembly including at least one pump body and a manifold, the manifold being connected to the pump body via a connecting pipe; a swing arm assembly, the swing arm assembly including: a first mounting plate, a first position adjusting device connected to the first mounting plate, the glue-making device having a first direction and a third direction, the first direction and the third direction intersecting each other, the first position adjusting device being used to adjust the position of the first mounting plate in the third direction, the manifold being fixed to the first mounting plate; and a second mounting plate, the second mounting plate being connected to a power module, the power module being used to adjust the position of the second mounting plate in the first direction, the first mounting plate being fixed to the second mounting plate via the first position adjusting device.
[0005] In some embodiments, the power module includes a first motor, a first track connected to the first motor, a first mounting block connected to the first track, the first mounting block sliding along the first track under the drive of the first motor, and a second mounting plate fixed to the first mounting block.
[0006] In some embodiments, the swing arm assembly includes a third mounting plate having a groove extending along a first direction, and a second mounting plate passing through the groove and fixed to the first mounting block.
[0007] In some embodiments, the swing arm assembly includes a first position recognition mechanism, the first position recognition mechanism including a first recognition element and a first blocking plate, the recognition element being able to recognize the position of the first blocking plate; the first blocking plate being fixed to the first mounting block or the second mounting plate.
[0008] In some embodiments, the glue-making device further includes a glue-making frame, which is mounted on a tray, and a second position identification mechanism is provided on one side of the tray for identifying the position of the tray.
[0009] In some embodiments, the second position identification mechanism includes a second identification element and a second blocking element, wherein the second identification element is used to identify the position of the second blocking element, and one end of the second blocking element contacts the tray.
[0010] In some embodiments, the second identification element is an optical coupler, the second blocking element is an optical coupler block, and the second blocking element is fitted with a spring element.
[0011] In some embodiments, the liquid dispensing assembly includes five pump bodies.
[0012] In some embodiments, the first position adjustment device is an adjustment slide.
[0013] In some embodiments, the glue generator further includes an interface component, which includes an adapter and a filter.
[0014] Beneficial Effects: This application provides a glue-making device, which includes a liquid dispensing assembly comprising at least one pump body and a manifold plate connected to the pump body via a connecting pipe; and a swing arm assembly comprising: a first mounting plate and a first position adjusting device connected to the first mounting plate, the glue-making device having a first direction and a third direction intersecting therein, the first position adjusting device being used to adjust the position of the first mounting plate in the third direction, the manifold plate being fixed to the first mounting plate; and a second mounting plate connected to a power module, the power module being used to adjust the position of the second mounting plate in the first direction, the first mounting plate being fixed to the second mounting plate via the first position adjusting device. The swing arm assembly of this glue-making device can adjust its height according to different situations, and the operating mechanism of this glue-making device can ensure glue-making stability. Attached Figure Description
[0015] Figure 1This is a schematic diagram of the liquid addition assembly in the embodiments of this application;
[0016] Figure 2 This is a schematic diagram of the structure of the glue-forming apparatus in the embodiments of this application;
[0017] Figure 3 This is a schematic diagram of the swing arm assembly in an embodiment of this application;
[0018] Figure 4 This is a schematic diagram of the swing arm assembly in an embodiment of this application;
[0019] Figure 5 This is a top view of the glue-making apparatus in an embodiment of this application;
[0020] Figure 6 This is a schematic diagram of the glue-making frame and tray structure in an embodiment of this application;
[0021] Figure 7 This is a schematic diagram of the interface component in an embodiment of this application. Detailed Implementation
[0022] The following description, in conjunction with the accompanying drawings, further illustrates the scheme of this application.
[0023] like Figure 1 and Figure 2 As shown, the glue-making apparatus of this application embodiment includes a liquid-adding assembly 10, which includes at least one pump body 101 and a manifold 102. The manifold 102 is connected to the pump body 101 through a connecting pipe 103.
[0024] To address the issue of uneven accelerator addition and subsequent gel inconsistencies caused by orders of magnitude differences in the dosage of coagulants and other liquids in existing technologies, this application includes a liquid addition component 10 comprising five pumps 101 in some embodiments. These five pumps 101 solve the problem of reagent mixing in existing technologies, ensuring that reagents are quantitatively mixed with the instrument via the pumps 101, avoiding tedious manual operations and increasing the accuracy of liquid mixing. The five pumps 101 can deliver polymer solutions and buffer solutions of different concentrations, ensuring that the dosage of different reagents is similar and preventing gel inconsistencies.
[0025] In some embodiments, the three pumps in this application are precisely speed-controlled pumps that respectively add high-concentration reagents, low-concentration reagents, and gel buffer solutions premixed with coagulants. By controlling the real-time flow ratio of the high-concentration and low-concentration reagents, the mixing ratio of the two different concentration reagents is controlled, thereby achieving a concentration gradient. Another pump 101 in this application is used to add the upper layer gel, which, together with the upper layer gel reagent, enables the one-step preparation of layered gels. The remaining pump 101 in this application can be used to deliver rinsing solution, allowing for continuous gel preparation. Furthermore, the liquid addition component 10 in this application can use the same set of reagents to prepare several different types of gels.
[0026] In some embodiments, the manifold 102 of this application is used to mix the liquid delivered from the pump body 101 and then deliver it through the mixing needle 104.
[0027] like Figure 2 As shown, the glue-forming apparatus of this application has a first direction X, a second direction Y, and a third direction Z, wherein the first direction X, the second direction Y, and the third direction Z intersect each other. Figure 3 and Figure 4 As shown, in some embodiments, the swing arm assembly 20 includes a first mounting plate 201, which is fixedly connected to the busbar 102, and the busbar 102 can move with the first mounting plate 201. The swing arm assembly 20 of this application also includes a first position adjustment device 202, which is used to adjust the position of the first mounting plate 201 in the third direction Z. In some specific embodiments, the first position adjustment device 202 of this application is a hand-held adjustment slide, which can adjust the position of the busbar 102 according to actual needs, thereby adjusting the position of the mixing needle 104.
[0028] The swing arm assembly 20 in this embodiment further includes a second mounting plate 203. The busbar 102 is fixed to the second mounting plate 203 via the first mounting plate 101 and the first position adjustment device 202. The second mounting plate 201 is connected to the power module 210. The power module 210 is used to adjust the position of the second mounting plate 203 in the first direction X, thereby adjusting the position of the busbar 102 and the mixing needle 104 located below the busbar 102.
[0029] like Figure 3 , Figure 4 and Figure 5 As shown, the power module 210 in this embodiment includes a first motor 211, a first track 212 connected to the first motor 211, a first mounting block 213 connected to the first track 212, the first mounting block 213 sliding along the first track 212 under the drive of the first motor 211, the first track 212 extending along the first direction X; and a second mounting plate 203 fixed to the first mounting block 213.
[0030] The swing arm assembly 20 of this application embodiment also includes a third mounting plate 204, on which a groove 205 extending along the first direction X is provided, and a second mounting plate 203 passes through the groove 205 and is fixed to the first mounting block 213.
[0031] To enable the glue applicator to identify the position of the mixing needle 104, the swing arm assembly 20 in this embodiment further includes a first position identification mechanism 220. The first position identification mechanism 220 includes a first identification element 221 and a first blocking plate 222. The identification element 221 can identify the position of the first blocking plate 222. The first blocking plate 212 is fixed to a first mounting block 213 or a second mounting plate 203. In some specific embodiments, the first identification element 221 is fixed by a fixing seat 223, and the position of the mixing needle 104 is determined by identifying the position of the first blocking plate 222 fixed to the first mounting block 213. This application provides two first identification elements 221 at each end of the slide groove 205, and the first identification element 221 can be an optocoupler.
[0032] like Figure 6 As shown, the glue-making apparatus of this application also includes a glue-making frame 30, which is mounted on a tray 40. A second position recognition mechanism 50 is provided on one side of the tray 40, and the second position recognition mechanism 50 is used to identify the position of the tray 40. In some embodiments, the second position recognition mechanism 50 includes a second recognition element 501 and a second blocking element 502. The second recognition element 501 is used to identify the position of the second blocking element 502, and one end of the second blocking element 502 contacts the tray 40. In some specific embodiments, the second recognition element 501 is an optocoupler, and the second blocking element 502 is an optocoupler block. The second blocking element 502 is fitted with a spring element 503. The glue-making frame 30 of this application is placed on the tray 40 and pushes the optocoupler block to block the optocoupler from receiving a signal, ensuring that the position of the glue-making frame 30 is stable during the glue-making process.
[0033] like Figure 7 As shown, in some embodiments, the glue-making device of this application further includes an interface component 60, which includes an adapter 601 and a filter 602. The adapter in the interface component 60 of this application is a USB adapter, and the interface component 60 has a built-in fuse for overload protection.
[0034] The glue-making apparatus of this application also includes a circuit structure and control components for realizing the operation of the glue-making apparatus of this application.
Claims
1. A glue-forming apparatus, characterized in that, The glue-forming device includes: A liquid filling assembly (10) includes at least one pump body (101) and a manifold (102), wherein the manifold (102) is connected to the pump body (101) via a connecting pipe (103); A swing arm assembly (20), the swing arm assembly (20) comprising: A first mounting plate (201), a first position adjustment device (202) connected to the first mounting plate (201), the glue applicator having a first direction (X) and a third direction (Z), the first direction (X) and the third direction (Z) intersecting, the first position adjustment device (202) being used to adjust the position of the first mounting plate (201) in the third direction (Z), and the busbar (102) being fixed to the first mounting plate (201); The second mounting plate (203) is connected to the power module (210). The power module (210) is used to adjust the position of the second mounting plate (203) in the first direction (X). The first mounting plate (201) is fixed to the second mounting plate (203) by the first position adjustment device (202).
2. The glue-forming apparatus according to claim 1, characterized in that, The power module (210) includes a first motor (211), a first track (212) connected to the first motor (211), a first mounting block (213) connected to the first track (212), and the first mounting block (213) sliding along the first track (212) under the drive of the first motor (211); and a second mounting plate (203) fixed to the first mounting block (213).
3. The glue-forming apparatus according to claim 2, characterized in that, The swing arm assembly (20) includes a third mounting plate (204) having a groove (205) extending along a first direction (X) and a second mounting plate (203) passing through the groove (205) and fixed to the first mounting block (213).
4. The glue-forming apparatus according to claim 2, characterized in that, The swing arm assembly (20) includes a first position recognition mechanism (220), which includes a first recognition element (221) and a first blocking plate (222). The first recognition element (221) can recognize the position of the first blocking plate (222). The first blocking plate (222) is fixed to the first mounting block (213) or the second mounting plate (203).
5. The glue-forming apparatus according to claim 1, characterized in that, It also includes a glue-making frame (30), which is installed on a tray (40). A second position identification mechanism (50) is provided on one side of the tray (40), which is used to identify the position of the tray (40).
6. The glue-forming apparatus according to claim 5, characterized in that, The second position identification mechanism (50) includes a second identification element (501) and a second blocking element (502). The second identification element (501) is used to identify the position of the second blocking element (502). One end of the second blocking element (502) is in contact with the tray (40).
7. The glue-forming apparatus according to claim 6, characterized in that, The second identification element (501) is an optocoupler, the second blocking element (502) is an optocoupler block, and the second blocking element (502) is fitted with a spring element (503).
8. The glue-forming apparatus according to claim 1, characterized in that, The liquid addition assembly (10) includes five pump bodies (101).
9. The glue-forming apparatus according to claim 1, characterized in that, The first position adjustment device (202) is an adjustment slide.
10. The glue-making apparatus according to claim 1, characterized in that, It also includes an interface component (60), which includes an adapter (601) and a filter (602).