Protein gravity column purification device

Abstract

The utility model relates to the technical field of protein purification, in particular to a protein gravity column purification device which comprises a purification box, a centralized storage rack and a filling table positioned on one side of the centralized storage rack, the purification box comprises an upper layer and a lower layer, a plurality of upper fixing holes for suspending purification columns are reserved in the upper layer, and a plurality of lower fixing holes for placing protein solution collecting pipes are reserved in the lower layer; a plurality of layers of placing cavities are separated in the centralized storage rack; by integrating the purification box, the centralized storage rack, the filling table, the corresponding pushing mechanism and the purification box grabbing mechanism, the automatic operation of the protein purification process is realized. And the purification box grabbing mechanism can accurately grab the purification box and transfer the purification box into the corresponding placing cavity. By means of the design, the purification operation efficiency is improved, the difficulty and errors of manual operation are reduced, and the stability and reliability of the purification process are ensured.

Description

Technical Field

[0001] This utility model relates to the field of protein purification technology, specifically a protein gravity column purification device. Background Technology

[0002] In the field of protein purification, gravity column purification is a commonly used method. This method typically involves passing a solution containing the target protein through a purification column packed with specific packing material. Gravity forces the solution to flow slowly through the column, thereby achieving protein separation and purification. Traditional protein gravity column purification operations often require manual intervention, including suspending the purification column on a suitable device, adding the protein solution, and collecting the purified protein. However, this manual operation is not only inefficient but also prone to introducing human error, affecting the purification results.

[0003] To address this issue, automated protein gravity column purification devices have emerged on the market. These devices typically include components such as a support for suspending the purification column, a pump for adding protein solution, and a collection tube for collecting the purified protein. However, these devices still have shortcomings in terms of automation and ease of operation. For example, some devices require operators to manually place the purification column and collection tube in the appropriate positions, which not only increases the difficulty of operation but also limits the level of automation. Utility Model Content

[0004] This invention addresses the technical problems existing in the prior art by providing a protein gravity column purification device to solve the problems mentioned in the background art.

[0005] The technical solution of this utility model to solve the above-mentioned technical problems is as follows: a protein gravity column purification device, including a purification box, a centralized storage rack, and a packing platform located on one side of the centralized storage rack;

[0006] The purification box includes upper and lower layers. The upper layer has several upper fixing holes for suspending the purification column, and the lower layer has several lower fixing holes for placing the protein solution collection tube.

[0007] The centralized storage rack is equipped with multiple storage cavities;

[0008] The packing platform is equipped with a purification box moving seat and a pushing mechanism connected to the purification box moving seat. The pushing mechanism is used to push the purification box moving seat carrying the purification box to the side of the centralized storage rack placement cavity.

[0009] The centralized storage rack is also equipped with a purification box gripping mechanism, which is used to grip the purification box on the purification box moving seat and transfer it to the corresponding placement cavity.

[0010] Based on the above technical solution, the present invention can be further improved as follows.

[0011] Furthermore, the purification cartridge gripping mechanism includes:

[0012] Two sets of grippers arranged opposite each other;

[0013] A gripper arm cylinder connected to the gripper jaws, the gripper arm cylinder being used to drive two sets of grippers to move closer or further apart;

[0014] Vertical and horizontal moving components connected to the grippers;

[0015] The push plate directly opposite the gripper's position;

[0016] A pusher cylinder connected to a pusher plate.

[0017] Furthermore, the bottom edge of the gripper is provided with an inwardly retracting support block.

[0018] Furthermore, both the vertical and horizontal moving components are electrically driven linear guides.

[0019] The push mechanism further includes:

[0020] A guide rail laid out in a horizontal direction;

[0021] A slide mounted on a guide rail and connected to the purification box moving seat;

[0022] A slide cylinder used to drive the movement of the slide.

[0023] Furthermore, the centralized storage rack is equipped with a removable guard plate along the material retrieval side of the placement cavity, and the guard plate is provided with a handle.

[0024] Furthermore, the protein gravity column purification device provided by this invention has at least the following beneficial effects compared with the prior art:

[0025] By integrating purification cartridges, a centralized storage rack, a packing station, and corresponding pushing and holding mechanisms, the protein purification process is automated. The pushing mechanism drives the purification cartridge carrier and its cartridges to the placement chamber of the centralized storage rack, while the holding mechanism accurately picks up the cartridges and transfers them to their corresponding placement chambers. This design not only improves the efficiency of the purification operation but also reduces the difficulty and error of manual operation, ensuring the stability and reliability of the purification process.

[0026] The gripper mechanism of the purification box features inwardly retractable blocks in its claws, increasing the contact area and clamping force with the purification box, thereby improving the stability and accuracy of gripping. Simultaneously, the centralized storage rack is equipped with a removable protective plate along the material handling side of the placement chamber. The protective plate has handles for easy gripping and removal or installation by operators, allowing for quick access to the purification box within the placement chamber when not in use or requiring maintenance. Attached Figure Description

[0027] Figure 1 This is a schematic diagram of the overall structure of the utility model;

[0028] Figure 2 This is a schematic diagram of the overall structure of the present invention from another perspective;

[0029] Figure 3 This is a partial structural diagram of the present invention;

[0030] Figure 4 This is a schematic diagram of the purification box of this utility model.

[0031] In the accompanying drawings, the components represented by the reference numerals are as follows:

[0032] 1. Purification box; 1.1. Upper fixing hole; 1.2. Lower fixing hole; 2. Centralized storage rack; 3. Filling platform; 4. Purification box moving seat; 5. Pushing mechanism; 5.1. Guide rail; 5.2. Slide seat; 5.3. Slide seat cylinder; 6. Purification box gripping mechanism; 6.1. Gripper; 6.2. Grip arm cylinder; 6.3. Vertical moving component; 6.4. Horizontal moving component; 6.5. Push plate; 6.6. Pushing cylinder; 6.7. Support block; 7. Protective plate; 7.1. Handle. Detailed Implementation

[0033] The principles and features of this utility model are described below with reference to the accompanying drawings. The examples given are only for explaining this utility model and are not intended to limit the scope of this utility model.

[0034] It should be noted that, unless otherwise explicitly specified and limited, the terms "installation," "connection," and "joining" should be interpreted broadly. For example, they can refer to fixed connections, detachable connections, or integrally formed structures. Those skilled in the art can understand the specific meaning of these terms in this patent based on the specific circumstances.

[0035] like Figure 1-Figure 4 As shown, the protein gravity column purification device of this practical design includes a purification box 1, a centralized storage rack 2, and a packing platform 3 located on one side of the centralized storage rack 2.

[0036] The purification box 1 includes two layers, an upper layer with several upper fixing holes 1.1 for suspending the purification column, and a lower layer with several lower fixing holes 1.2 for placing the protein solution collection tube.

[0037] The centralized storage rack 2 is equipped with multiple storage cavities;

[0038] The filling station 3 is provided with a purification box moving seat 4 and a pushing mechanism 5 connected to the purification box moving seat 4. The pushing mechanism 5 is used to push the purification box moving seat 4 carrying the purification box 1 to the side of the central storage rack 2 placement cavity.

[0039] The centralized storage rack 2 is also equipped with a purification box gripping mechanism 6, which is used to grip the purification box 1 on the purification box moving seat 4 and transfer it to the corresponding placement cavity.

[0040] The packing station 3 is equipped with a purification box moving seat 4 and a connected pushing mechanism 5. The pushing mechanism 5 can drive the purification box moving seat 4 and the purification box 1 on it to move to the placement cavity next to the centralized storage rack 2. At the same time, the centralized storage rack 2 is also equipped with a purification box gripping mechanism 6, which can grip the purification box 1 on the purification box moving seat 4 and accurately transfer it to the corresponding placement cavity in the centralized storage rack 2, thereby completing the automated operation of the entire purification process.

[0041] In one embodiment, the purification cartridge gripping mechanism 6 includes:

[0042] Two sets of opposing grippers 6.1;

[0043] A gripper cylinder 6.2 connected to the gripper 6.1 is used to drive the two sets of grippers 6.1 to move closer or further apart from each other;

[0044] Vertical moving component 6.3 and horizontal moving component 6.4 are connected to gripper 6.1;

[0045] The pusher plate 6.5 is directly opposite the gripper 6.1.

[0046] Pushing cylinder 6.6 is connected to pusher plate 6.5.

[0047] Specifically, the bottom edge of the gripper 6.1 is provided with an inwardly retracting support block 6.7.

[0048] To hold the purification box 1 more stably, the bottom edge of the gripper 6.1 is also designed with an inwardly contracting support block 6.7 to increase the contact area and clamping force with the purification box 1.

[0049] As a supplement, both the vertical moving component 6.3 and the horizontal moving component 6.4 are electrically driven linear guides.

[0050] The gripper 6.1 is connected to the vertical moving component 6.3 and the horizontal moving component 6.4. Both components are electrically driven linear guides, enabling the gripper 6.1 to move in the vertical and horizontal directions, respectively. Through these two movements, the purification box gripping mechanism 6 can accurately move the gripped purification box 1 from the purification box moving seat 4 to the corresponding placement cavity of the centralized storage rack 2.

[0051] In addition, to assist the gripper 6.1 in better gripping and releasing the purification box 1, the mechanism is also equipped with a pusher plate 6.5 facing the gripper 6.1. The pusher plate 6.5 can push the purification box 1 forward by the drive of the pusher cylinder 6.6, ensuring that the gripper 6.1 can smoothly grip or release the purification box 1.

[0052] As one implementation method, the pushing mechanism 5 includes:

[0053] 5.1 Guide rails laid out in the horizontal direction;

[0054] A slide 5.2 is mounted on the guide rail 5.1 and connected to the purification box moving seat 4;

[0055] Slide cylinder 5.3 is used to drive the slide 5.2 to move.

[0056] The sliding cylinder 5.3 pushes or pulls the sliding block 5.2 to move horizontally along the guide rail 5.1 through the extension and retraction of its piston rod, thereby driving the purification box moving seat 4 and the purification box 1 on it to the designated position.

[0057] In one embodiment, the centralized storage rack 2 is equipped with a removable guard plate 7 along the material retrieval side of the placement cavity, and the guard plate 7 is provided with a handle 7.1.

[0058] The main purpose of this design is to protect the purification box 1 inside the placement chamber from external interference or damage, while providing convenient material retrieval. The protective plate 7 is equipped with a handle 7.1, which allows operators to easily grasp and remove or install the protective plate 7, thereby enabling quick access to the purification box 1 inside the placement chamber when not in use or when maintenance is required.

[0059] It should be noted that, in this document, the terms "comprising," "including," or any other variations thereof are intended to cover a non-exclusive inclusion, such that a process, method, article, or apparatus that comprises a list of elements includes not only those elements but also other elements not expressly listed, or elements inherent to such a process, method, article, or apparatus. Unless otherwise expressly specified and limited, the terms "installed," "connected," or "linked" should be interpreted broadly, for example, as a fixed connection, a detachable connection, or an integral connection; a mechanical connection or an electrical connection; a direct connection or an indirect connection via an intermediate medium; or a connection within two elements. Those skilled in the art can understand the specific meaning of the above terms in this invention according to the specific circumstances.

[0060] Although preferred embodiments of the present invention have been described, those skilled in the art, upon learning the basic inventive concept, can make other changes and modifications to these embodiments. Therefore, the appended claims are intended to be interpreted as including the preferred embodiments as well as all changes and modifications falling within the scope of the present invention.

[0061] Obviously, those skilled in the art can make various modifications and variations to this utility model without departing from its spirit and scope. Therefore, if these modifications and variations fall within the scope of the claims of this utility model and their equivalents, this utility model also intends to include these modifications and variations.

Claims

1. A protein gravity column purification device, characterized in that, It includes a purification box (1), a central storage rack (2), and a packing station (3) located on one side of the central storage rack (2); The purification box (1) includes two layers, an upper layer with several upper fixing holes (1.1) for suspending the purification column, and a lower layer with several lower fixing holes (1.2) for placing the protein solution collection tube. The centralized storage rack (2) is divided into multiple storage cavities; The filling station (3) is provided with a purification box moving seat (4) and a pushing mechanism (5) connected to the purification box moving seat (4). The pushing mechanism (5) is used to push the purification box moving seat (4) carrying the purification box (1) to the side of the central storage rack (2) placement cavity. The centralized storage rack (2) is also equipped with a purification box grasping mechanism (6), which is used to grasp the purification box (1) on the purification box moving seat (4) and transfer it to the corresponding placement cavity.

2. The protein gravity column purification apparatus according to claim 1, characterized in that, The purification cartridge gripping mechanism (6) includes: Two sets of grippers arranged opposite each other (6.1); A gripper cylinder (6.2) connected to the gripper (6.1) is used to drive the two sets of grippers (6.1) to move closer or further apart from each other; A vertical moving component (6.3) and a horizontal moving component (6.4) connected to the gripper (6.1); The pusher plate (6.5) is directly opposite the gripper (6.1) position; Push cylinder (6.6) connected to pusher plate (6.5).

3. The protein gravity column purification apparatus according to claim 2, characterized in that, The bottom edge of the gripper (6.1) is provided with an inwardly retracting support block (6.7).

4. The protein gravity column purification apparatus according to claim 2, characterized in that, Both the vertical moving component (6.3) and the horizontal moving component (6.4) are electrically driven linear guides.

5. The protein gravity column purification apparatus according to claim 1, characterized in that, The push mechanism (5) includes: Guide rails laid out in the horizontal direction (5.1); A slide (5.2) mounted on a guide rail (5.1) and connected to the purification box moving seat (4); Slide cylinder (5.3) for driving the slide (5.2) to move.

6. The protein gravity column purification apparatus according to claim 1, characterized in that, The centralized storage rack (2) is equipped with a removable guard plate (7) along the material retrieval side of the placement cavity, and the guard plate (7) is provided with a handle (7.1).

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