Large-volume extraction plate for cell sorting

Abstract

The utility model provides a large-volume extraction plate for cell sorting, which comprises seven hole sites which are sequentially ranked from one end to seven, the first hole is a magnetic bar sleeve hole, the second hole is a sample adding hole, the third hole is a magnetic bead liquid hole, the fourth hole to the sixth hole are washing liquid holes, and the seventh hole is an eluent hole. The device can be matched with an extractor, the magnetic bar sleeve is grabbed and embedded in the extractor, then the magnetic bar sleeve is driven to be transferred to the magnetic bead liquid hole to rotate and mix magnetic bead liquid, and after washing is completed, a magnetic bar in the extractor extends into the magnetic bar sleeve to be magnetically separated and carries magnetic beads to be transferred to a subsequent sample incubation and washing liquid hole. The whole process is highly automatic, the cell sorting efficiency is improved, and the operation risk caused by manpower and personnel operation is reduced.

Description

Technical Field

[0001] This utility model relates to the field of nucleic acid detection technology, and in particular to a large-volume extraction plate for cell sorting. Background Technology

[0002] In cell sorting experiments, the enrichment of target cells mainly involves various techniques such as density gradient centrifugation, magnetic activation cell sorting, fluorescence activation cell sorting, and microfluidics. Among these, magnetic activation cell sorting is the simplest, most time-saving, and most affordable method, and it can also separate multiple samples simultaneously.

[0003] However, current techniques mostly involve manual operation by laboratory personnel. When sorting large volumes of blood samples for cell separation, the processing becomes time-consuming and labor-intensive. Currently, there is an extraction instrument, such as... Figure 1 As shown, it can accommodate one to six extraction plates at a time, enabling automated and rapid cell sorting experiments via the extraction instrument. Therefore, there is an urgent need to design a large-volume extraction plate compatible with this extraction instrument. Utility Model Content

[0004] The purpose of this invention is to provide a large-volume extraction plate for cell sorting, which can be adapted to an extraction instrument to achieve automated cell sorting and solve the problem of time-consuming and laborious blood sample processing during manual cell sorting experiments.

[0005] The objective of this utility model is achieved through the following technical solution:

[0006] A large-volume extraction plate for cell sorting includes seven wells, numbered one to seven from one end. Well one is a magnetic rod well, well two is a sample loading well, well three is a magnetic bead solution well, wells four to six are washing solution wells, and well seven is an elution solution well.

[0007] Preferably, the overall dimensions of the large-volume extraction plate are: 11cm in length, 1.2cm in width, and 6cm in height.

[0008] Preferably, the interval between two adjacent holes is 0.1 cm; the depth of the magnetic rod sleeve hole, sample loading hole, magnetic bead liquid hole and elution liquid hole is 4.5 cm; the depth of the washing liquid hole is 5.5 cm; the diameter of the magnetic rod sleeve hole is 1.2 cm; the diameter of the sample loading hole is 2.5 cm; and the diameters of the magnetic bead liquid hole, washing liquid hole and elution liquid hole are all 1.5 cm.

[0009] This invention is compatible with an extraction instrument, which includes a mechanism for grasping and fitting a magnetic rod sleeve. The sample, along with the magnetic rod sleeve, is then transferred to a magnetic bead solution well for rotational mixing. After washing, the magnetic rod extends into the sleeve for magnetic separation, carrying the magnetic beads to subsequent sample incubation and washing solution wells. The entire process is highly automated, improving cell sorting efficiency and reducing manual labor and operational risks. Attached Figure Description

[0010] Figure 1 The diagram shows the structure of the extraction instrument in the background technology of this utility model.

[0011] Figure 2 This is a schematic diagram of the structure of a large-volume extraction plate for cell sorting according to the present invention.

[0012] The annotations in the attached figures are explained as follows:

[0013] 1: Magnetic rod sleeve hole, 2: Sample loading hole, 3: Magnetic bead liquid hole, 4: Washing solution hole, 5: Elution solution hole, 6: Extraction plate. Detailed Implementation

[0014] The embodiments of this disclosure will now be described in detail with reference to the accompanying drawings.

[0015] The following specific examples illustrate the implementation of this disclosure. Those skilled in the art can easily understand the advantages and effects of this disclosure from the content disclosed in this specification. Obviously, the described embodiments are only a part of the embodiments of this disclosure, and not all of them. This disclosure can also be implemented or applied through other different specific embodiments, and the details in this specification can also be modified or changed based on different viewpoints and applications without departing from the spirit of this disclosure. It should be noted that, in the absence of conflict, the following embodiments and features in the embodiments can be combined with each other. Based on the embodiments in this disclosure, all other embodiments obtained by those skilled in the art without creative effort are within the scope of protection of this disclosure.

[0016] Example 1

[0017] This invention is compatible with an extraction instrument, which includes a magnetic rod holder for grasping and fitting the magnetic rod. The sample is then transferred to the magnetic bead solution well 3 with the magnetic rod holder attached for rotational mixing (i.e., washing). After washing, the magnetic rod extends into the magnetic rod holder inside the extraction instrument for magnetic separation and transfers the magnetic beads to the subsequent sample incubation and washing solution well 5. The entire process is highly automated, improving cell sorting efficiency and reducing manual labor and operational risks. Negative samples are retained in the sample loading well 2, and positive samples are retained in the elution solution well 5.

[0018] like Figure 2As shown, a large-volume extraction plate for cell sorting includes seven wells, numbered one to seven from one end. Well number one is the magnetic rod sleeve well 1, well number two is the sample loading well 2, well number three is the magnetic bead liquid well 3, wells four to six are washing liquid wells 4, and well number seven is the elution liquid well 5.

[0019] Furthermore, the overall dimensions of the large-volume extraction plate are: 11cm in length, 1.2cm in width, and 6cm in height.

[0020] Furthermore, the interval between two adjacent holes is 0.1 cm; the depth of the magnetic rod sleeve hole 1, sample loading hole 2, magnetic bead liquid hole 3, and elution liquid hole 5 is 4.5 cm; the depth of the washing liquid hole 4 is 5.5 cm; the diameter of the magnetic rod sleeve hole 1 is 1.2 cm; the diameter of the sample loading hole 2 is 2.5 cm; and the diameters of the magnetic bead liquid hole 3, washing liquid hole 4, and elution liquid hole 5 are all 1.5 cm.

[0021] One extraction plate can sort 10-40mL of blood cells at a time. The extractor can be adapted to six extraction plates for extraction, and can sort up to 240mL of blood cells at a time. The sorting process is aseptic and automated, which effectively improves experimental efficiency and reduces experimental risks and contamination.

[0022] In the description of this utility model, it should be understood that the terms "middle", "length", "upper", "lower", "front", "rear", "vertical", "horizontal", "inner", "outer", "radial", "circumferential", etc., indicate the orientation or positional relationship based on the orientation or positional relationship shown in the accompanying drawings. They are only for the convenience of describing this utility model and simplifying the description, and do not indicate or imply that the device or element referred to must have a specific orientation, or be constructed and operated in a specific orientation. Therefore, they should not be construed as limitations on this utility model.

[0023] In this invention, unless otherwise expressly specified and limited, the first feature "on" the second feature may be in direct contact with the first feature, or indirect contact with the first feature through an intermediate medium. "A plurality of" means at least two, such as two, three, etc., unless otherwise expressly and specifically limited.

[0024] In this utility model, unless otherwise explicitly specified and limited, the terms "installation," "connection," "joining," and "fixing," etc., should be interpreted broadly. For example, they can refer to a fixed connection, a detachable connection, or an integral part; they can refer to a mechanical connection, an electrical connection, or a connection that allows communication between them; they can refer to a direct connection or an indirect connection through an intermediate medium; they can refer to the internal communication of two components or the interaction between two components, unless otherwise explicitly limited. Those skilled in the art can understand the specific meaning of the above terms in this utility model according to the specific circumstances.

[0025] The above description is merely illustrative of the embodiments of this utility model and is not intended to limit the scope of this utility model. For those skilled in the art, any modifications, equivalent substitutions, improvements, etc., made within the spirit and principles of this utility model without creative labor should be included within the protection scope of this utility model.

Claims

1. A large-volume extraction plate for cell sorting, characterized in that, It includes seven wells, numbered one to seven from one end. Well number one is the magnetic rod sleeve well (1), well number two is the sample loading well (2), well number three is the magnetic bead liquid well (3), wells four to six are washing liquid wells (4), and well number seven is the elution liquid well (5).

2. The large-volume extraction plate for cell sorting as described in claim 1, characterized in that, The overall dimensions of the large-volume extraction plate are: 11cm in length, 1.2cm in width, and 6cm in height.

3. The large-volume extraction plate for cell sorting as described in claim 1, characterized in that, The interval between two adjacent holes is 0.1 cm; the depth of the magnetic rod sleeve hole (1), sample loading hole (2), magnetic bead liquid hole (3) and elution liquid hole (5) is 4.5 cm; the depth of the washing liquid hole (4) is 5.5 cm; the diameter of the magnetic rod sleeve hole (1) is 1.2 cm; the diameter of the sample loading hole (2) is 2.5 cm; the diameter of the magnetic bead liquid hole (3), washing liquid hole (4) and elution liquid hole (5) is 1.5 cm.

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