Microporous percolation protein detection chip

Abstract

The utility model discloses a microporous percolation protein detection chip, which belongs to the technical field of biochips and diagnostic reagents, and comprises an upper shell and a lower shell, the upper shell is connected with the lower shell in a clamping manner, a microporous membrane is arranged between the upper shell and the lower shell, the upper surface of the upper shell is provided with a concave cavity, a sample introduction window is arranged in the cavity, and the sample introduction window is connected with the microporous membrane. A sample introduction window is formed in the upper surface of the lower shell, a sample introduction hole is formed in the sample introduction window, the lower end of the sample introduction hole is connected with a sample introduction channel, the bottom of the sample introduction channel is connected with a reaction area on the microporous membrane, the lower end of the reaction area is connected with a sample outlet channel, and the lower end of the sample outlet channel is connected with a sample outlet hole in the upper surface of the lower shell. The protein chip disclosed by the utility model can reduce the use amount of samples, is accurate in measurement result, and does not cause environmental pollution.

Description

Technical Field

[0001] This utility model relates to the field of biochip and diagnostic reagent technology, and in particular to a microporous permeation protein detection chip. Background Technology

[0002] Traditional percolation-based chips typically consist of multiple membrane layers, including a nitrocellulose membrane, a reverse osmosis layer, an absorbent layer, and a leak-proof layer. They are widely used in clinical testing because they allow for control of reaction time and process. However, the membrane layers of these traditional percolation-based chips are generally designed as rectangular or square, with the detection area at one end and the sample application area at the other. This results in a significant waste of sample solution during the flow to the sample application area.

[0003] Another patent of our company, CN 219134884 U, discloses a dotted gold immunofiltration kit, which includes a top cover made of non-absorbent material, a microporous membrane coated with capture probes, an absorbent pad, and a bottom cover made of non-absorbent material, arranged sequentially. The top cover has a circular spotting window. The microporous membrane is circular, with 12 detection points evenly distributed around its outer circumference. By attaching the microporous membrane to the circular spotting window, the sample solution can be directly dripped onto the detection points of the microporous membrane, effectively reducing sample waste. However, in practical applications, this patent still suffers from problems such as sample waste, inability to recover waste liquid, and detection interference. Utility Model Content

[0004] The technical problem to be solved by this invention is to design a novel microporous permeation protein detection chip that can not only accurately add samples and prevent interference between detection results, but also realize the recycling of waste liquid and eliminate environmental pollution.

[0005] To solve the above-mentioned technical problems, the present invention adopts the following technical solution: a microporous permeation protein detection chip, comprising an upper shell and a lower shell, the upper shell and the lower shell being snapped together, a microporous membrane being disposed between the upper shell and the lower shell, a recessed cavity being provided on the upper surface of the upper shell, a sample injection window being provided in the cavity, a sample injection hole being provided on the sample injection window, the lower end of the sample injection hole being connected to a sample injection channel, the bottom of the sample injection channel being connected to a reaction zone on the microporous membrane, the lower end of the reaction zone being connected to an outlet channel, and the lower end of the outlet channel being connected to an outlet hole on the upper surface of the lower shell.

[0006] Furthermore, the number of injection ports is not less than two, and each injection port corresponds to its own injection channel, reaction zone, discharge channel, and discharge port.

[0007] Furthermore, a groove is provided on the side of the lower housing, and a protrusion is provided at the lower end of the upper housing, the protrusion engaging with the groove.

[0008] Furthermore, after the upper and lower housings are engaged, clamps are installed to secure the engagement point.

[0009] Furthermore, the cross-sections of the upper shell, lower shell, microporous membrane, and clamp are all circular.

[0010] Furthermore, a collection device is fixedly connected to the lower end of the lower housing, and the collection device is in communication with the sample outlet.

[0011] Furthermore, the collecting device is a cavity, including a connecting area and a supporting area, wherein the connecting area and the supporting area are in communication.

[0012] Preferably, the bottom of the support area is enclosed.

[0013] Preferably, a through hole is provided at the bottom of the support area, and the support area is connected to the liquid pump.

[0014] Furthermore, the reaction zone is pre-loaded with antibodies or antigens. Beneficial effects

[0015] This application incorporates a sample dispensing channel at the bottom of the sample dispensing port, with the bottom of the channel in contact with the reaction zone. This allows the sample to directly reach the reaction zone and participate in the reaction, eliminating sample waste during its flow through the microporous membrane. Simultaneously, the waste liquid can flow into a collection device through the sample outlet channel, achieving waste liquid collection without causing environmental pollution or posing a risk of infection to operators.

[0016] This application features an ingenious design that uses only one reaction membrane, breaking through the traditional multi-layer membrane design of percolation chips and having significant implications for the protein chip detection industry. Attached Figure Description

[0017] Figure 1 This is a structural schematic diagram of Embodiment 1 of the present utility model.

[0018] Figure 2 This is a schematic diagram of the liquid flow channel of this utility model.

[0019] Figure 3 This is a structural schematic diagram of Embodiment 2 of the present invention.

[0020] Figure 4 This is a top view of the assembled microporous permeation protein detection chip of this utility model.

[0021] Wherein 1-upper shell, 11-protrusion, 12-cavity, 2-lower shell, 21-groove, 3-sample inlet window, 31-sample inlet hole, 32-sample inlet channel, 4-microporous membrane, 41-reaction zone, 42-sample outlet channel, 43-sample outlet hole, 44-collection device, 441-connection area, 442-support area, 5-clamp. Detailed Implementation

[0022] To enhance understanding of this utility model, it will be described in detail below with reference to the embodiments and accompanying drawings. These embodiments are only used to explain this utility model and do not limit the scope of protection of this utility model. Example 1

[0023] like Figure 1 The diagram shows a microporous protein detection chip, comprising an upper shell 1 and a lower shell 2 with a circular cross-section. Several protrusions 12 at the lower end of the upper shell 1 and several grooves 21 on the side of the lower shell 2 can engage. After the upper shell 1 and lower shell 2 are engaged, a circular microporous membrane 4 can be fixed between the upper shell 1 and the lower shell 2.

[0024] The upper surface of the upper shell 1 is provided with a recessed cavity 12, and a sample inlet window 3 is provided in the cavity 12. A plurality of sample inlet holes 31 are provided on the sample inlet window 3. Figure 1 It can be configured with 4 injection ports (31), or 6, 8, etc., with a maximum or minimum of 2 ports. Set it as needed.

[0025] like Figure 2 As shown, each injection well 31 is connected to an injection channel 32 at its lower end. The bottom of each injection channel 32 is connected to a reaction zone 41 on the microporous membrane 4. The antigen or antibody of the sample to be tested and the corresponding quality control protein are pre-embedded on the reaction zone 41.

[0026] The lower end of each reaction zone 41 is connected to a corresponding sample outlet channel 42, and the lower end of the sample outlet channel 42 is connected to the sample outlet hole 43 on the upper surface of the lower housing 2.

[0027] The lower end of the sample outlet 43 is connected to the collection device 44, which is a cavity that can be integrally formed with the lower housing 2 or detachably fixedly connected to the lower housing 2.

[0028] The collecting device 44 includes a connecting area 441 and a supporting area 442, which are connected. The connecting area 441 is connected to the lower housing 2, which has a circular cross-section. To accommodate the shape of the lower housing 2, the outer wall of the connecting area 441 can be designed with a certain curvature. The supporting area 442 is designed as a cylinder to keep the chip stable. However, the specific shape of the collecting device 44 is not limited here, as long as its function is guaranteed.

[0029] The bottom of the collection device 44 can be either sealed or have a through hole. When sealed, the waste liquid is stored in the collection device 44 and can be discarded after use. When a through hole is provided, the collection device needs to be connected to a liquid pump to remove the waste liquid for centralized disposal. The advantage of connecting to the liquid pump is that it allows control of the liquid flow rate, enabling more precise control of the reaction time.

[0030] During testing, the sample is dropped into the injection port and flows directly into the reaction zone through the injection channel to react with the antigen or antibody on the reaction zone. The waste liquid after the reaction flows into the collection device through the sample outlet channel for collection, which can save a lot of samples. Example 2

[0031] The difference from Embodiment 1 is that, after the upper housing 1 and the lower housing 2 are engaged, a clamp 5 is provided to secure the engagement point. The clamp 5 is shaped as follows: Figure 3 As shown, the assembled top view is as follows Figure 4 As shown. Clamp 5 can also be other shapes, as long as the upper and lower shells are tightened after engagement.

[0032] The embodiments disclosed herein are preferred embodiments, but are not limited thereto. Those skilled in the art can readily grasp the spirit of this utility model based on the above embodiments and make different extensions and variations. However, as long as they do not depart from the spirit of this utility model, they are all within the protection scope of this utility model.

Claims

1. A microporous permeation protein detection chip, comprising an upper shell (1) and a lower shell (2), wherein the upper shell (1) and the lower shell (2) are engaged and connected, and a microporous membrane (4) is disposed between the upper shell (1) and the lower shell (2), characterized in that, The upper surface of the upper shell (1) is provided with a recessed cavity (12), and a sample inlet window (3) is provided in the cavity (12). A sample inlet hole (31) is provided on the sample inlet window (3). The lower end of the sample inlet hole (31) is connected to the sample inlet channel (32). The bottom of the sample inlet channel (32) is connected to the reaction zone (41) on the microporous membrane (4). The lower end of the reaction zone (41) is connected to the sample outlet channel (42). The lower end of the sample outlet channel (42) is connected to the sample outlet hole (43) on the upper surface of the lower shell (2).

2. The microporous permeation protein detection chip according to claim 1, characterized in that, The number of injection ports (31) is not less than 2, and each injection port (31) corresponds to its own injection channel (32), reaction zone (41), discharge channel (42) and discharge port (43).

3. The microporous permeation protein detection chip according to claim 1, characterized in that, The lower housing (2) has a groove (21) on its side and the upper housing (1) has a protrusion (11) at its lower end. The protrusion (11) engages with the groove (21).

4. The microporous permeation protein detection chip according to claim 3, characterized in that, After the upper shell (1) and the lower shell (2) are engaged, a clamp (5) is installed to tighten the engagement.

5. The microporous permeation protein detection chip according to claim 4, characterized in that, The cross-sections of the upper shell (1), lower shell (2), microporous membrane (4), and clamp (5) are all circular.

6. The microporous permeation protein detection chip according to claim 1, characterized in that, The lower end of the lower housing (2) is fixedly connected to a collection device (44), and the collection device (44) is connected to the sample outlet (43).

7. The microporous permeation protein detection chip according to claim 6, characterized in that, The collecting device (44) is a cavity, including a connecting area (441) and a supporting area (442), wherein the connecting area (441) and the supporting area (442) are connected.

8. The microporous permeation protein detection chip according to claim 7, characterized in that, The bottom of the support area (442) is closed.

9. The microporous permeation protein detection chip according to claim 7, characterized in that, The bottom of the support area (442) is provided with a through hole, and the support area (442) is connected to the liquid pump.

10. The microporous permeation protein detection chip according to claim 1, characterized in that, The reaction zone (41) is preloaded with antibodies or antigens.

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