Constant-temperature shaking table for in-vitro transcription reaction

Abstract

The utility model discloses a constant-temperature shaking table for in-vitro transcription reaction. The constant-temperature shaking table comprises a bin body with an opening in one side, a base is installed in the bin body, a supporting frame is installed on the upper side of the base, and a driving device for driving the supporting frame to shake is arranged in the center of the base. A grid layer is arranged on the upper side of the supporting frame, a plurality of elastic belts are further installed on the supporting frame, and the two ends of each elastic belt are fixed to the opposite sides of the supporting frame respectively and used for tightening a container. A reaction bag is flatly placed on the upper side of a supporting frame and is not fixed by a fixing device, and sliding friction occurs between the supporting frame and the contact surface of the reaction bag after opening, so that the internal shaking degree of the reaction bag is low, and the reaction effect cannot be met. The elastic belt is installed on the supporting frame and has the contraction performance, the reaction bag is tightened through the elastic belt to be bound after being placed, and the reaction bag and the supporting frame are made to shake synchronously.

Description

TECHNICAL FIELD

[0001] The utility model belongs to the technical field of biological reaction equipment, especially relates to a constant temperature shaking table for in vitro transcription reaction. BACKGROUND

[0002] There are many types of vaccine drugs, among which inactivated antigens (such as inactivated viruses or bacteria) are one of the traditional vaccine forms. In recent years, mRNA vaccines as a new type of vaccine technology have attracted widespread attention and application. mRNA vaccines introduce mRNA encoding specific antigens into the human body, allowing human cells to transcribe these mRNAs and produce corresponding antigen proteins, thereby stimulating the immune system to produce an immune response. This technology has the advantages of high efficiency, speed and relative safety, especially in responding to sudden epidemics, it can quickly develop new vaccines. The mRNA vaccine preparation process is roughly as follows: usually using E. coli as a carrier, plasmid DNA (pDNA) is obtained through fermentation, alkaline lysis and other processes, mRNA is obtained after enzyme digestion and in vitro transcription of plasmid DNA, and the product is finally prepared through lipid nanoparticle (LNP) technology.

[0003] After plasmid DNA is digested and transcribed in vitro to obtain mRNA, it needs to be carried out at room temperature. In order to prevent external interference during transcription, a disposable reaction bag is used, and the product is used once to avoid cross-infection. The reaction bag is a plastic bag body, which is packaged and placed in a constant temperature shaking table. The existing constant temperature shaking table lacks a fixing device for the reaction bag, which causes the shaking table bottom and the reaction bag to slide, and the solution inside the reaction bag is not mixed uniformly. SUMMARY

[0004] The utility model aims at providing a constant temperature shaking table for in vitro transcription reaction, which solves the problem of uneven mixing of the solution inside the reaction bag caused by the sliding of the shaking table bottom and the reaction bag.

[0005] To solve the above technical problems, the utility model is realized by the following technical solutions:

[0006] A constant temperature shaking table for in vitro transcription reaction, comprising a one-side opening bin body, a bin door is installed on the opening side of the bin body, the bin door is hingedly installed, a base is installed in the bin body, a support frame is installed on the upper side of the base, a driving device for driving the support frame to shake is arranged in the center of the base;

[0007] A grid layer is arranged on the upper side of the support frame for supporting containers, and a plurality of elastic belts are also installed on the support frame, the elastic belts are in strip structure, and the two ends of the elastic belts are fixed to the opposite sides of the support frame respectively for tightening the containers.

[0008] Further, the elastic band corresponds to the inner side of the storage body and has a rod sleeve, and corresponds to the front side of the storage body and has a hook, the support frame is provided with a slide rod on the inner and outer sides, the elastic band rod sleeve is sleeved on the slide rod, and the hook is detachably connected with the slide rod.

[0009] The side of the support frame is provided with a receiving groove for accommodating the elastic band.

[0010] Further, the support frame comprises a support plate arranged at the bottom of the grid layer, and a clamping layer is arranged between the support plate and the grid layer, and the support plate is provided with a sponge pad layer.

[0011] A spring is arranged between the support plate bottom and the base corresponding to the corner position, and is used for elastic support.

[0012] The utility model discloses the following beneficial effects: because the reaction bag is of flexible material, has no fixed shape, and the liquid filling amount also determines that the fixed clamping device cannot be used to fix the reaction bag, and the reaction bag is placed on the upside of the support frame without being fixed by the fixing device, after being opened, the contact surface of the support frame and the reaction bag slides and rubs, the inside of the reaction bag shakes to a low degree, and the reaction effect cannot be satisfied. BRIEF DESCRIPTION OF DRAWINGS

[0013] In order to more clearly illustrate the technical scheme of the embodiments of the utility model, the following will briefly introduce the drawings needed to be used in the embodiment description.

[0014] Figure 1 The utility model discloses a structure schematic view.

[0015] Figure 2 The utility model discloses a support frame part structure schematic view.

[0016] Figure 3 The utility model discloses an elastic band dismounting structure schematic view.

[0017] In the drawings, the components represented by each sign are listed as follows: storage body 1, storage door 11, base 12, support frame 2, grid layer 21, elastic band 3, rod sleeve 31, hook 32, slide rod 5, receiving groove 51, support plate 22, sponge pad layer 23, spring 4. DETAILED DESCRIPTION

[0018] The technical scheme in the embodiments of the utility model will be clearly and completely described in combination with the drawings in the embodiments of the utility model, and obviously, the described embodiments are only part of the embodiments of the utility model, not all the embodiments.

[0019] As Figures 1-2As shown: a constant temperature shaker for in vitro transcription reaction, including a single opening warehouse body 1, the warehouse body is a cubic structure, the side wall is a shell, and a control panel is arranged at the same time, which is used for controlling the reaction temperature, shaking frequency and other parameters in the warehouse body. The warehouse body 1 is provided with a warehouse door 11 corresponding to the opening side, the warehouse door has a metal frame and a central organic glass, which is convenient to close the warehouse door, so that the outside can see the internal situation, the warehouse door 11 is hinged, the base 12 is installed in the warehouse body 1, the support frame 2 is installed on the upper side of the base 12, and the drive device for driving the support frame to shake is arranged in the center of the base 12; the support frame is a plate-shaped support frame, which is arranged horizontally, the drive device is a click with vertical shaft, the click drive end is a crankshaft, the support frame bottom has a shaft sleeve matched with the crankshaft, and the support frame is driven by the motor to move in a circular motion in the horizontal direction.

[0020] The upper side of the support frame 2 is provided with a grid layer 21, which is formed by splicing the steel bars in the horizontal and vertical directions to form a square groove body for supporting the container. At the same time, small specification containers such as test tubes and centrifugal tubes can be placed in the groove body of the grid structure. The support frame 2 is also provided with a plurality of elastic belts 3, which are strip-shaped structures. The two ends of the elastic belt 3 are fixed to the opposite sides of the support frame, respectively, for tightening the container.

[0021] The container for in vitro transcription reaction is made of disposable reaction bag to prevent external interference during transcription. The product is disposable and can avoid cross infection. The reaction bag is a plastic bag body, which is energy-saving and environmentally friendly. Since the reaction bag is made of flexible material and has no fixed shape, and the liquid volume also determines that it cannot be fixed by a fixed clamping device. The reaction bag is placed on the upper side of the support frame without fixing device. After opening, the contact surface between the support frame and the reaction bag slides and rubs, causing the reaction bag to shake to a low degree, which cannot meet the reaction effect. Therefore, the elastic belt is installed on the support frame, which has a contraction performance. After the reaction bag is placed, it is tightened and bound by the elastic belt, so that the shaking of the reaction bag and the support frame is synchronized.

[0022] As shown in Figure 3 The elastic belt 3 has a rod sleeve 31 corresponding to the inner side of the warehouse body 1 and a hook 32 corresponding to the front side of the warehouse body 1. The support frame 2 is provided with a slide rod 5 on the inner and outer sides. The rod sleeve 31 is sleeved on the corresponding slide rod 5, and the hook 32 is detachably connected with the corresponding slide rod 5. By sliding the rod sleeve on the slide rod, the position of the elastic belt can be adjusted. Through the coordination of multiple elastic belts, the reaction bag is fixed. The support frame 2 side has a receiving groove 51 for accommodating the elastic belt 3. It is used to accommodate the unused elastic belt without occupying the support area of the grid layer.

[0023] As shown in Fig. 2: the support frame 2 includes a support plate 22 at the bottom of the grid layer, and the grid layer 21 and the support plate 22 also have a sandwich layer, and the support plate 22 has a sponge pad layer 23. The support plate is used in cooperation with the grid layer, and the sponge pad layer is deformed after bearing to improve the stability of the support. The bottom of the support plate 22 is provided with a spring 4 corresponding to the position of the corner and the base 12, which is used for elastic support. The spring provides elastic support, and does not affect the shaking trajectory when the support plate shakes.

[0024] The embodiments are selected and described in detail in the specification, in order to better explain the principles and practical applications of the utility model, so that the persons skilled in the art can well understand and utilize the utility model.

Claims

1. A constant temperature shaker for in vitro transcription reactions, characterized in that: The application relates to a storage cabinet with a single-sided opening, wherein a cabinet door (11) is hingedly installed on the opening side of the cabinet (1), a base (12) is installed in the cabinet (1), a support frame (2) is installed on the upper side of the base (12), and a driving device for driving the support frame to sway is arranged in the center of the base (12). A grid layer (21) is arranged on the upper side of the support frame (2) and used for supporting containers; and a plurality of elastic belts (3) are installed on the support frame (2) and used for tightening the containers.

2. The constant temperature shaker for in vitro transcription reactions according to claim 1, characterized in that: The elastic belt (3) is provided with a rod sleeve (31) corresponding to the inner side of the cabinet (1) and a hook (32) corresponding to the front side of the cabinet (1); sliding rods (5) are arranged on the inner and outer sides of the support frame (2); the rod sleeve (31) is sleeved on the corresponding sliding rod (5); and the hook (32) is detachably matched with the corresponding sliding rod (5).

3. The constant temperature shaker for in vitro transcription reactions according to claim 2, characterized in that: The side of the support frame (2) is provided with a receiving groove (51) for accommodating the elastic belt (3).

4. The constant temperature shaker for in vitro transcription reactions of claim 1, wherein: The support frame (2) comprises a support plate (22) arranged at the bottom of the grid layer; the grid layer (21) and the support plate (22) are further provided with a sandwich layer; and the support plate (22) is provided with a sponge pad layer (23).

5. The constant temperature shaker for in vitro transcription reactions according to claim 4, characterized in that: Springs (4) are arranged between the bottom of the support plate (22) and the base (12) corresponding to the corner positions and used for elastically supporting.

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