Automatic agarose gel making instrument

Abstract

The utility model discloses an automatic agarose gel making instrument which comprises an instrument body, a heating base arranged at the bottom of one side of the instrument body, and a container tank placed on the heating base, and is characterized in that a tank cover is arranged at the top of the container tank, a motor is arranged at the upper end of the tank cover, a fixedly connected stirring rod is arranged at the lower end of the motor, and the stirring rod extends into the container tank; a plurality of stirring blades A and a plurality of stirring blades B are upwards arranged on the stirring rod from the bottom end, the stirring blades A and the stirring blades B are alternately arranged at intervals, and the structures of the stirring blades A and the stirring blades B form a vertical mirror image. The motor is arranged in a motor box at the top end of the tank cover. According to the automatic agarose gel making instrument, the stirring rod is provided with a plurality of groups of vertical mirror image stirring blades, so that the pushing directions of liquid flow are rich, a low-efficiency stirring area does not exist, and even if a container is large and the amount of a solution is large, the uniformity and sufficiency of stirring can be ensured.

Description

Technical Field

[0001] This utility model relates to the technical field of automated agarose gel casting apparatus, specifically an automated agarose gel casting apparatus. Background Technology

[0002] Agarose gel electrophoresis is a routine technique in molecular biology, and its operation generally involves four steps: gel preparation, sample loading, electrophoresis, and result observation. Agarose gel preparation begins with the preparation of a sol, typically using diluted electrode buffer as a solvent, adding a certain mass of agarose, and heating and stirring until the agarose dissolves to form a sol (gel solution). Key control points include temperature and stirring speed. During heating, temperature and time must be strictly controlled to avoid overheating and decomposition of the agarose; stirring should be thorough and uniform to prevent clumping. Currently, agarose gel preparation is mainly done manually, which involves heating and stirring, a process that is time-consuming and labor-intensive, and it is difficult to guarantee uniform and thorough stirring.

[0003] To address this issue, CN206710374U discloses a novel automated agarose gel preparation apparatus. By designing a heating module and a stirring paddle, it achieves thorough heating and simultaneous mixing of the gel solution during preparation. However, the stirring paddle in this design employs a traditional, simple design with only one blade. During stirring, the blade pushes the solution in a single direction. Furthermore, if the container is large or the solution volume is high, the single blade creates an inefficient stirring zone, making it difficult to thoroughly mix the entire solution. This fails to meet the high requirements for uniformity and thoroughness in agarose gel solution mixing. Utility Model Content

[0004] The purpose of this invention is to provide an automated agarose gel casting apparatus to solve the problems mentioned in the background art.

[0005] To solve the above-mentioned technical problems, this utility model provides the following technical solution:

[0006] This utility model discloses an automated agarose gel preparation apparatus, comprising an instrument body, a heating base located at the bottom of one side of the instrument body, and a container placed on the upper part of the heating base. The container is characterized by having a lid on top, a motor at the upper end of the lid, and a fixedly connected stirring rod at the lower end of the motor. The stirring rod extends into the container, and from the bottom upwards, the stirring rod has several stirring blades A and several stirring blades B, which are alternately arranged at intervals. The structures of the stirring blades A and B form a vertical mirror image.

[0007] As an improvement, the motor is located inside a motor housing at the top of the can lid.

[0008] As an improvement, the container is equipped with a handle and a liquid outlet valve.

[0009] As an improvement, a temperature control zone is provided on one side of the heating base.

[0010] As an improvement, there are two of each of the stirring blades A and B, and the total height of several stirring blades A and B is greater than three-quarters of the depth of the container.

[0011] The advantages of this invention compared to existing technologies are as follows: Agarose and buffer solution are added to the container. The temperature and time are set in the operating temperature control zone. Then, the motor is started, and stirring blades A and B stir together. Because there are multiple stirring blades A and B, their total height exceeds most of the container's depth, eliminating inefficient stirring zones. Furthermore, their alternating arrangement and the vertical mirror image structure of adjacent stirring blades A and B ensure that the liquid flow is pushed in opposite directions during stirring. This results in multiple flow directions within the container, with mutual interference, guaranteeing uniform and thorough stirring. During the heating and dissolution process, the agarose is uniformly and thoroughly stirred until a sol-gel solution is formed.

[0012] This automated agarose gel casting apparatus features multiple sets of vertically mirrored stirring blades on the stirring rod, providing a variety of directions for pushing the liquid flow. There are no inefficient stirring zones, ensuring uniformity and thoroughness of stirring even in large containers and with large volumes of solution. Attached Figure Description

[0013] To more clearly illustrate the technical solutions in the embodiments of this utility model or the prior art, the drawings used in the description of the embodiments or the prior art will be briefly introduced below. Obviously, the drawings listed below are only some structural schematic diagrams of this utility model, and not all of them.

[0014] Figure 1 This is a schematic diagram of the structure of an automated agarose gel casting apparatus according to this utility model. Figure 1 .

[0015] Figure 2 This is a schematic diagram of the structure of an automated agarose gel casting apparatus according to this utility model. Figure 2 .

[0016] Figure 3 This is a cross-sectional view of an automated agarose gel casting apparatus according to this utility model.

[0017] Figure 4 This is a schematic diagram of the stirring blade of an automated agarose gel casting apparatus according to this utility model.

[0018] Figure label:

[0019] Instrument body 1; heating base 2; container 3; container lid 4; motor 5; stirring rod 6; stirring blade A 7; stirring blade B 8; motor box 9; handle 10; liquid outlet valve head 11; temperature control zone 12. Detailed Implementation

[0020] The technical solutions of the present utility model will be clearly and completely described below with reference to the accompanying drawings of the embodiments. Obviously, the described embodiments are only some embodiments of the present utility model, and not all embodiments. Based on the embodiments of the present utility model, all other embodiments obtained by those of ordinary skill in the art without creative effort are within the protection scope of the present utility model.

[0021] In the description of the embodiments of this utility model, it should be noted that if terms such as "center", "upper", "lower", "left", "right", "vertical", "horizontal", "inner", "outer" appear, the indicated orientation or positional relationship is based on the orientation or positional relationship shown in the accompanying drawings, or the orientation or positional relationship that the utility model product is usually placed in during use. They are only for the convenience of describing this utility model and simplifying the description, and do not indicate or imply that the device or element referred to must have a specific orientation, or be constructed and operated in a specific orientation. Therefore, they should not be construed as limitations on this utility model.

[0022] Furthermore, the terms "first," "second," and "third" are used only for distinguishing descriptions and should not be interpreted as indicating or implying relative importance. The use of terms such as "horizontal," "vertical," and "suspended" does not imply that the component must be absolutely horizontal or suspended, but rather that it can be slightly tilted. For example, "horizontal" simply means that its direction is more horizontal than "vertical," not that the structure must be perfectly horizontal, but can be slightly tilted.

[0023] In the description of the embodiments of this utility model, the terms "multiple" or "several" refer to at least two.

[0024] In the description of the embodiments of this utility model, it should also be noted that, unless otherwise explicitly specified and limited, the terms "set," "install," "connect," and "link" should be interpreted broadly. For example, they can refer to a fixed connection, a detachable connection, or an integral connection; they can refer to a mechanical connection or an electrical connection; they can refer to a direct connection or an indirect connection through an intermediate medium; and they can refer to the internal connection of two components. Those skilled in the art can understand the specific meaning of the above terms in this utility model according to the specific circumstances.

[0025] This embodiment is combined with the appendix Figures 1 to 4 This paper provides a detailed description of an automated agarose gel casting apparatus.

[0026] This embodiment discloses an automated agarose gel casting apparatus, comprising an instrument body 1, a heating base 2 at the bottom of one side of the instrument body 1, and a container 3 placed on the upper part of the heating base 2. The container 3 is characterized by having a lid 4 at the top, a motor 5 at the upper end of the lid 4, and a fixedly connected stirring rod 6 at the lower end of the motor 5. The stirring rod 6 extends into the container 3, and the stirring rod 6 has several stirring blades A7 and B8 arranged alternately from bottom to top. The structures of the stirring blades A7 and B8 form a vertical mirror image.

[0027] The motor 5 is located inside the motor box 9 at the top of the can lid 4.

[0028] The container 3 is equipped with a handle 10 and a liquid outlet valve 11. The liquid outlet valve 11 is used to discharge the gel solution.

[0029] The heating base 2 has a temperature control zone 12 on one side.

[0030] There are two of each of the stirring blades A7 and B8, and the total height of the stirring blades A7 and B8 is greater than three-quarters of the depth of the container tank 3.

[0031] In practice, agarose and buffer solution are added to container 3. The temperature and time are set in the operating temperature control zone 12. Then, motor 5 is started, and stirring blades A7 and B8 stir together. Since there are multiple stirring blades A7 and B8, their total height exceeds most of the depth of container 3, eliminating inefficient stirring zones. Furthermore, they are spaced and alternately arranged, with adjacent stirring blades A7 and B8 forming a vertical mirror image. During stirring, they push the liquid flow in opposite directions, resulting in multiple flow directions within container 3 that interfere with each other, ensuring uniform and thorough stirring. During the heating and dissolution process, the agarose is uniformly and thoroughly stirred until a sol (gel solution) is formed.

[0032] The present invention and its embodiments have been described above. This description is not restrictive, and the actual scope of protection is not limited thereto. Any variations or substitutions that can be easily conceived by those skilled in the art within the technical scope disclosed in this invention should be included within the scope of protection of this invention. Therefore, the scope of protection of this invention should be determined by the scope of the claims.

Claims

1. An automated agarose gel casting apparatus, comprising an instrument body (1), wherein a heating base (2) is provided at the bottom of one side of the instrument body (1), and a container (3) is placed on the upper part of the heating base (2), characterized in that, The container (3) is provided with a lid (4) on the top. The lid (4) is provided with a motor (5) at the upper end. The motor (5) is provided with a fixedly connected stirring rod (6) at the lower end. The stirring rod (6) extends into the container (3). The stirring rod (6) is provided with several stirring blades A (7) and several stirring blades B (8) from the bottom to the top. The stirring blades A (7) and B (8) are arranged alternately at intervals. The structures of the stirring blades A (7) and B (8) form a vertical mirror image.

2. The automated agarose gel casting apparatus according to claim 1, characterized in that, The motor (5) is located in the motor box (9) at the top of the can lid (4).

3. The automated agarose gel casting apparatus according to claim 1, characterized in that, The container (3) is equipped with a handle (10) and a liquid outlet valve (11).

4. The automated agarose gel casting apparatus according to claim 1, characterized in that, The heating base (2) has a temperature control zone (12) on one side.

5. An automated agarose gel casting apparatus according to claim 1, characterized in that, There are two of each of the stirring blades A (7) and B (8), and the total height of several stirring blades A (7) and B (8) is greater than three-quarters of the depth of the container (3).

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