Temperature calibration device capable of being used for different reaction plates

Abstract

The temperature calibration device capable of being used for different reaction plates comprises a temperature measurement module and a plug, the temperature measurement module comprises a temperature sensor, the temperature sensor comprises a temperature measurement head and a wire, the plug is a high-temperature-resistant elastomer and is provided with a through hole, and the temperature sensor penetrates through the through hole; an insulating layer is arranged outside the wire, the whole wire is of a spring structure and used for being tightly attached to the inner wall of the reaction hole, the temperature measuring head is used for making contact with the bottom of the reaction hole, and the plug is used for blocking the upper portion of the reaction hole. The reaction plate temperature measuring device can adapt to different reaction plates, and the temperature measuring accuracy can be improved.

Description

TECHNICAL FIELD

[0001] The utility model relates to the field of measurement technology, especially relates to a temperature calibration device for different reaction plates. BACKGROUND

[0002] Reaction plate is a common consumable of biochemical instrument, and the equipment using reaction plate is also various, such as fluorescence reaction PCR instrument, nucleic acid extractor, constant temperature oscillator, coagulation analyzer, enzyme label instrument and other experimental equipment or medical instrument, and different manufacturers have different specifications and different models. According to the number of reaction holes, it is divided into 96-hole plate, 48-hole plate, 16-hole plate and the like; according to the hole type, the reaction plate has square hole, round hole and the like; and the size such as hole diameter, hole spacing and thickness is applied in different equipment, and there are also differences, for example, the 96-hole plate of Thermo Fisher has 0.2ml and 0.1ml two specifications.

[0003] At present, the equipment using reaction plate needs to configure an independent temperature measurement unit for each reaction hole when doing temperature measurement, for example, PCR instrument temperature calibration instrument can be used for temperature calibration of fluorescence reaction PCR instrument, but cannot be used for temperature calibration of nucleic acid extractor. UTILITY MODEL CONTENT

[0004] In order to solve the above problems of prior art, the utility model provides a temperature calibration device for different reaction plates, which can adapt to the temperature calibration of all reaction holes.

[0005] In order to achieve the above purpose, the utility model adopts the technical scheme that:

[0006] Firstly, the utility model provides a temperature calibration device for different reaction plates, which comprises a temperature measurement module and a plug, the temperature measurement module comprises a temperature sensor, the temperature sensor comprises a temperature measurement head and a wire, the plug is a high-temperature-resistant elastomer and is provided with a through hole, and the temperature sensor passes through the through hole.

[0007] The wire is provided with an insulating layer outside and is in a spring structure as a whole, is used for closely adhering to the inner wall of the reaction hole, the temperature measurement head is used for contacting the bottom of the reaction hole, and the plug is used for plugging the upper part of the reaction hole.

[0008] The utility model discloses beneficial effect lies in: the whole wire is spring structure, can carry out spiral close -fit with the inner wall of reaction hole, one hand adapts the size of different reaction hole to adapt to different reaction plate, on the other hand, through the contact of wire and reaction hole inner wall, make the wire temperature of temperature sensor close to the temperature in reaction hole, and the temperature measuring point is close to the wire temperature difference, and heat exchange is also smaller, reduces the influence of wire heat conduction to temperature measuring position, improves temperature measuring accuracy. Meanwhile, the plug fills the upper portion of reaction hole, reduces the heat flow, and the wire of spring structure is in compression state, makes the temperature measuring head close to the bottom of reaction hole, improves the heat transfer efficiency, to improve temperature measuring accuracy.

[0009] Optionally, the pitch of the wire in the lower part of the reaction hole is smaller than the pitch of the upper part.

[0010] According to the above description, the lower part of the wire of the temperature sensor is close to the temperature measuring point position, which is more likely to cause heat exchange and affect the temperature measuring accuracy, therefore, the number of turns is increased, so that the lower part of the wire of the temperature sensor is close to the temperature of the measuring point, thereby further improving the temperature measuring accuracy.

[0011] Optionally, the diameter of the spiral of the wire changes according to the shape of the reaction hole.

[0012] Optionally, the temperature measuring module further comprises a signal conversion module, a control unit, a power module and a remote transmission module, the power module is electrically connected with the temperature sensor, the signal conversion module, the control unit and the remote transmission module, and the control unit is electrically connected with the signal conversion module and the remote transmission module respectively.

[0013] Optionally, the remote transmission module is a Wi-Fi module.

[0014] Optionally, the temperature measuring head of the temperature sensor is a thermocouple or a platinum resistance.

[0015] Optionally, each turn of the wire can be heated and fixed. BRIEF DESCRIPTION OF DRAWINGS

[0016] Fig. 1 It is the cooperation schematic view of temperature sensor and reaction hole related to the utility model embodiment;

[0017] Fig. 2 It is the cooperation schematic view of temperature calibration device and host computer for different reaction plates of the utility model embodiment.

[0018] MARK NO.

[0019] 1, temperature sensor;11, wire;12, temperature measuring head;

[0020] 2, plug;21, through hole;

[0021] 3. signal conversion module;

[0022] 4. control unit;

[0023] 5. power module;

[0024] 6. remote transmission module;

[0025] 100. host computer;

[0026] 200. temperature measurement module;

[0027] 300. reaction well. DETAILED DESCRIPTION

[0028] In order to better understand the above technical solutions, the exemplary embodiments of the present application will be described in more detail below with reference to the accompanying drawings. Although the exemplary embodiments of the present application are shown in the drawings, it should be understood that the present application can be implemented in various forms and should not be limited by the embodiments described herein. On the contrary, these embodiments are provided to enable a clearer, more thorough understanding of the present application and to convey the complete scope of the present application to those skilled in the art.

[0029] Embodiment one

[0030] In actual application scenarios, the reaction plate is placed in a fluorescence reaction PCR instrument, a nucleic acid extractor, a constant temperature oscillator, a coagulation analyzer, an enzyme marker instrument, and other experimental equipment or medical instruments. After the heating module of the experimental equipment is heated, a temperature field is formed in the reaction plate, providing temperature generation conditions for the chemical biological reaction in the reaction well. Therefore, temperature calibration in the reaction well is necessary. However, due to the different sizes and shapes of the reaction plate holes, the temperature measurement module is numerous. Therefore, the present embodiment solves the above problems by tightly attaching the wire to the inner wall of the reaction well in a spiral manner.

[0031] Please refer to Figs. 1-2 The temperature calibration device for different reaction plates provided by the present embodiment includes a temperature measurement module 200 and a plug 2. The temperature measurement module 200 includes a temperature sensor 1, the temperature sensor 1 includes a temperature measurement head 12 and a wire 11, the plug 2 is a high-temperature-resistant elastomer and has a through hole 21, and the temperature sensor 1 passes through the through hole 21. The wire 11 is provided with an insulating layer on the outside and has a spring structure as a whole, which is used to tightly adhere to the inner wall of the reaction well 300. The temperature measurement head 12 is used to contact the bottom of the reaction well 300, and the plug 2 is used to block the upper part of the reaction well 300.

[0032] In the present embodiment, the plug 2 is a high-temperature-resistant elastic material, such as PVC plastic, silicone, etc., which can be adapted to the upper part of the reaction well 300 of different sizes to block the upper part of the reaction well 300 and reduce the heat flow.

[0033] The through hole 21 is arranged in the middle region of the plug 2.

[0034] The temperature sensing head 12 of the temperature sensor 1 is a thermocouple or a platinum resistance.

[0035] The wire 11 has an insulating layer outside, avoiding short circuit caused by close contact of the wire 11.

[0036] The turns of the wire 11 can be heated and fixed, to ensure the stability of the structure.

[0037] Thus, the temperature sensing head 12 of the temperature sensor 1 is used to contact the bottom of the reaction hole 300, to measure the bottom temperature of the reaction hole 300. However, since the wire 11 has heat conduction, if the wire 11 is pulled out of the reaction hole 300 in a straight line, due to the large temperature difference between the inside of the reaction hole 300 and the environment, for example, 90℃ inside the reaction hole 300 and 23℃ of the environment, the temperature difference between the two ends of the wire 11 is large, and the metal conducts heat quickly, which greatly affects the temperature field of the temperature measuring point, and cannot accurately measure the temperature at the temperature measuring point. Therefore, in the embodiment, the wire 11 is in a spring structure, and is in contact with the inner wall of the reaction hole 300 as much as possible, so that the temperature of the wire 11 of the temperature sensor 1 is close to the temperature inside the reaction hole 300, the temperature difference between the temperature measuring point and the wire 11 is small, the heat exchange is also small, the influence of heat conduction of the wire 11 on the temperature measuring position is reduced, and the temperature measuring accuracy is improved. Moreover, the spring structure produces elastic deformation, and the extrusion caused by the fixation of the plug 2 on the upper part of the reaction hole 300 makes the temperature measuring point of the temperature sensor 1 close to the bottom of the reaction hole 300, improving the heat conduction efficiency.

[0038] In the embodiment, the pitch of the wire 11 of the temperature sensor 1 in the lower part of the reaction hole 300 is smaller than that in the upper part. This is because the lower part of the wire 11 of the temperature sensor 1 is close to the temperature measuring point, which is more likely to cause heat exchange and affect the temperature measuring accuracy. Therefore, the number of turns is increased, so that the lower part of the wire 11 of the temperature sensor 1 is close to the temperature of the measuring point, thereby further improving the temperature measuring accuracy.

[0039] In the embodiment, the spiral diameter of the wire 11 of the temperature sensor 1 changes according to the shape of the reaction hole. Since the current reaction hole 300 is basically a cone, the diameter at the bottom is small, and the diameter gradually increases from the bottom to the upper part, so the spiral diameter of the wire 11 also gradually increases, but the spiral diameter corresponding to different shapes of the reaction hole 300 will change accordingly.

[0040] Reference Fig. 2It can be known that the temperature measuring module 200 further comprises a signal conversion module 3, a control unit 4, a power module 5 and a remote transmission module 6. The power module 5 is electrically connected with the temperature sensor 1, the signal conversion module 3, the control unit 4 and the remote transmission module 6. The control unit 4 is electrically connected with the signal conversion module 3 and the remote transmission module 6 respectively. The power module 5 is used for supplying power to the other modules as a whole. In the figure, only the control connection of the control unit 4 to the power module 5 is shown.

[0041] In the embodiment, the signal conversion module 3 comprises an AD converter, a signal amplifier and the like.

[0042] On this basis, the remote transmission module 6 in the embodiment is a Wi-Fi module.

[0043] Based on this, when the temperature calibration device for different reaction plates in the embodiment is used for temperature measurement of the reaction plate, the working process is as follows:

[0044] (1) The lead wire 11 in the spring structure is placed in the reaction hole 300, so as to tightly adhere to the inner wall of the reaction hole 300 and make the temperature measuring head 12 on the temperature sensor 1 contact the bottom of the reaction hole 300. Then, the plug 2 is used to block the upper part of the reaction hole 300.

[0045] (2) The reaction plate with the temperature sensor 1 plugged in the reaction hole 300 is placed in the device to be measured. The heating program of the device to be measured is set and started to run.

[0046] The device to be measured comprises a PCR instrument, a nucleic acid extractor and the like. After the heating program of the device to be measured is set and run, the temperature in the reaction hole 300 will start to change gradually.

[0047] (3) The real-time temperature signal of the bottom of the reaction hole 300 is converted into an electric signal by the temperature measuring head 12. The electric signal is transmitted to the control unit 4 to obtain a real-time temperature value. The real-time temperature value is transmitted to the upper computer 100.

[0048] (4) The real-time temperature value is displayed in real time. The device to be measured is calibrated according to the real-time temperature value.

[0049] Therefore, the working process of the upper computer 100 in the embodiment is also described as follows:

[0050] 1. Basic information management, including information of the entrusted unit and sample information. The user needs to create a detection project in accordance with the national PCR calibration regulations, fill in the information of the entrusted unit, and the related information of the sample to be detected, including the name of the unit, the contact person, the model specification and the like.

[0051] 2. The verification process management is realized by real-time data acquisition. The system communicates with the temperature measurement module 200 by Wi-Fi, and the sampling frequency can reach 100 milliseconds. The collected data is displayed in the form of real-time curve, so that the whole temperature change process can be intuitively understood. In addition, the data list is also displayed, so that the actual temperature value of each time period collected can be directly viewed.

[0052] 3. Data processing, including data correction and data calculation. The correction value of the temperature measurement sensor can be obtained by verification, and the correction is performed in real time after inputting the system. The average value, indication error, uncertainty, uniformity, average heating rate, average cooling rate and detection conclusion of the detection data are automatically calculated after each calibration.

[0053] 4. System management, including generating original records, user management, historical data query and system parameter setting. After calibration, original records conforming to the regulations need to be generated. The original records include the information of the inspection unit, the related information of the PCR instrument and the detection data. User management can add, delete and modify user information. Historical data query can query the detection projects done in the history, and can query the required detection project information according to the date, unit information, sample information and other related information, and generate original records. System parameter setting includes standard device and verifier setting information.

[0054] Therefore, the embodiment designs the lead wire 11 part of the temperature sensor 1, adds the plug 2, adapts different reaction plates, and improves the temperature measurement accuracy.

[0055] Therefore, it should be noted that the specific selection of each module in the embodiment is a specific example description, and in other equivalent embodiments, models that can meet the corresponding functions can be replaced.

[0056] In the description of the present application, it should be understood that the terms "first", "second" are only used for description purposes, and cannot be understood as indicating or implying relative importance or implicitly indicating the number of indicated technical features. Therefore, the features limited by "first", "second" can explicitly or implicitly include one or more features. In the description of the present application, the meaning of "multiple" is two or more, unless otherwise specifically limited.

[0057] In the utility model, unless another definite provision and limitation, the terms "mount", "link", "connect", "fix" and so on should do broad sense understanding, for example, can be fixed connection, also can be detachable connection, or be integrated;Can be mechanical connection, also can be electrical connection;Can be direct connection, also can pass through intermediate medium indirectly connect;Can be two element inside communication or two element mutual action relation.For ordinary skilled person in the art, can understand the specific meaning of above-mentioned terms in the utility model according to specific circumstances.

[0058] In the utility model, unless another definite provision and limitation, the terms "mount", "link", "connect", "fix" and so on should do broad sense understanding, for example, can be fixed connection, also can be detachable connection, or be integrated;Can be mechanical connection, also can be electrical connection;Can be direct connection, also can pass through intermediate medium indirectly connect;Can be two element inside communication or two element mutual action relation.For ordinary skilled person in the art, can understand the specific meaning of above-mentioned terms in the utility model according to specific circumstances.

[0059] In the description of the present application, the description of the terms "one embodiment", "some embodiments", "embodiment", "example", "specific example" or "some examples" means that the specific features, structures, materials or characteristics described in conjunction with the embodiment or example are included in at least one embodiment or example of the present application. In the present application, the illustrative description of the above terms does not necessarily refer to the same embodiment or example. Moreover, the specific features, structures, materials or characteristics described can be combined in any one or more embodiments or examples in a suitable manner. In addition, the skilled in the art can combine and combine the different embodiments or examples described in the present application and the features of different embodiments or examples without contradiction.

[0060] Although the embodiments of the present application have been shown and described above, it can be understood that the above-mentioned embodiments are exemplary and cannot be understood as limiting the present application. The ordinary skilled in the art can make changes, modifications, replacements and variations to the above-mentioned embodiments within the scope of the present application.

Claims

1. A temperature calibration device usable for different reaction plates, characterized in that, The temperature measuring module comprises a temperature sensor, and the temperature sensor comprises a temperature measuring head and a lead wire; and the plug is a high-temperature-resistant elastomer and is provided with a through hole, and the temperature sensor passes through the through hole. The lead wire is provided with an insulating layer outside and has a spring structure as a whole, and is used for closely adhering to the inner wall of the reaction hole; the temperature measuring head is used for contacting the bottom of the reaction hole; and the plug is used for plugging the upper part of the reaction hole.

2. The temperature calibration device for different reaction plates according to claim 1, wherein, The pitch of the lead wire in the lower part of the reaction hole is smaller than the pitch of the upper part.

3. The temperature calibration device for different reaction plates according to claim 1, wherein, The spiral diameter of the lead wire changes according to the shape of the reaction hole.

4. The temperature calibration device for different reaction plates according to any one of claims 1 to 3, characterized in that, The temperature measuring module further comprises a signal conversion module, a control unit, a power module and a remote transmission module; the power module is electrically connected with the temperature sensor, the signal conversion module, the control unit and the remote transmission module; and the control unit is electrically connected with the signal conversion module and the remote transmission module respectively.

5. The temperature calibration device for different reaction plates according to claim 4, wherein, The remote transmission module is a Wi-Fi module.

6. The temperature calibration device for different reaction plates according to any one of claims 1 to 3, characterized in that, The temperature measuring head of the temperature sensor is a thermocouple or a platinum resistance.

7. The temperature calibration device for different reaction plates according to any one of claims 1 to 3, characterized in that, The lead wire can be heated and fixed between each coil.

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