Liquid path system and flow cytometer

Abstract

The embodiment of the utility model provides a liquid path system and a flow cytometer, and relates to the technical field of flow cytometers. The liquid path system comprises a negative pressure tank, a flow chamber, a sampling needle, a swab and a sheath liquid bottle, wherein the negative pressure tank, the sampling needle and the sheath liquid bottle are all communicated with the flowing chamber, the swab is provided with a cleaning channel, and the sampling needle penetrates through the cleaning channel. In the working process, the negative pressure tank is used for providing negative pressure for the flowing chamber and enabling the sampling needle to suck a sample into the flowing chamber, the sheath liquid bottle is used for conveying sheath liquid to the flowing chamber and enabling the sheath liquid to wrap the sample to form laminar flow for optical detection, and the liquid path system replaces an injection pump and a plunger pump in the prior art to carry out the sample loading process. Pipeline connection is simplified, and the size of a liquid path system is reduced. Furthermore, the sampling needle can be conveniently cleaned through the cleaning channel arranged on the swab, so that cross contamination among samples and negative influence on a test result are avoided.

Description

TECHNICAL FIELD

[0001] The utility model relates to flow cytometer technical field, specifically, relate to a kind of liquid path system and flow cytometer. BACKGROUND

[0002] Flow cytometer is usually used for the analysis and statistics of cells or particles in sample. Flow cytometer is mainly composed of liquid path system, optical system, electronic system and data analysis system. As one of the important components of flow cytometer, the liquid path system transports sample liquid containing cells or particles labeled by fluorescent dye and sheath liquid to the flow chamber, and focuses the cells or particles using fluid dynamics principle, so that the cells or particles pass through the detection area of the flow chamber one by one.

[0003] In the prior art, syringe pumps and plunger pumps are used for sample loading process, which has the problems of complex pipeline connection and large liquid path volume. In addition, it is not convenient to clean the sampling needle. UTILITY MODEL CONTENT

[0004] The utility model provides a kind of liquid path system and flow cytometer, it can simplify pipeline connection, reduce liquid path system volume, and it is convenient to clean the sampling needle.

[0005] The embodiment of the utility model can be realized as follows:

[0006] In the first aspect, the utility model provides a kind of liquid path system, applicable to flow cytometer, the liquid path system includes: negative pressure tank, flow chamber, sampling needle, swab and sheath liquid bottle;

[0007] Wherein, the negative pressure tank, the sampling needle and the sheath liquid bottle are communicated with the flow chamber, the swab is equipped with cleaning channel, and the sampling needle is throughly arranged in the cleaning channel.

[0008] In optional implementation, the negative pressure tank includes negative pressure tank body and control valve, the negative pressure tank body is equipped with first flow path channel and pressure relief port, one end of the first flow path channel is communicated with the pressure relief port, and the other end of the first flow path channel is used to communicate with the negative pressure tank body, and the control valve is arranged in the first flow path channel.

[0009] In optional implementation, the negative pressure tank includes negative pressure tank body, first pressure sensor and / or damping device, the first pressure sensor is used to output the pressure parameter of the negative pressure tank body, and the damping device is arranged in the negative pressure tank body, and the damping device is communicated with the negative pressure tank body.

[0010] In an optional embodiment, the liquid path system further comprises a swab pump, one end of the cleaning channel is in communication with the swab pump, and the other end of the cleaning channel is in communication with the sheath liquid bottle.

[0011] In an optional embodiment, the liquid path system further comprises a waste liquid bottle and a waste liquid pump, and the waste liquid pump is in communication between the waste liquid bottle and the negative pressure tank.

[0012] In an optional embodiment, the sampling needle and the cleaning channel are relatively slidable.

[0013] In an optional embodiment, the liquid path system further comprises a sheath liquid pump, a degasser, and a first pipeline, and the sheath liquid bottle, the sheath liquid pump, the degasser, and the flow chamber are sequentially arranged in the first pipeline.

[0014] In an optional embodiment, the liquid path system further comprises a first filter, and the first filter is arranged in the first pipeline.

[0015] In an optional embodiment, the liquid path system further comprises a de-pulsation device, and the de-pulsation device is arranged in the first pipeline.

[0016] In an optional embodiment, the liquid path system further comprises a second filter, and the second filter is arranged in the first pipeline.

[0017] In an optional embodiment, the liquid path system further comprises a cleaning liquid pump and a cleaning liquid bottle, one end of the cleaning liquid pump is in communication with the cleaning liquid bottle, and the other end of the cleaning liquid pump is in communication with the flow chamber.

[0018] In an optional embodiment, the liquid path system further comprises a flow meter, and the flow meter is arranged on a pipeline between the sampling needle and the flow chamber.

[0019] In an optional embodiment, the liquid path system further comprises a second pressure sensor, and the second pressure sensor is arranged on a pipeline between the flow chamber and the sheath liquid bottle.

[0020] In a second aspect, the utility model provides a kind of flow cytometer, comprising the liquid path system of any one of the foregoing embodiments.

[0021] The liquid path system and flow cytometer of the embodiments of the utility model have the following beneficial effects, for example:

[0022] The utility model provides a kind of liquid path system, can be applicable to flow cytometer, in working process, negative pressure tank is used to provide negative pressure to flow chamber, and make sampling needle inhale sample into flow chamber, sheath liquid bottle is used to transport sheath liquid to flow chamber, so that sheath liquid is wrapped with sample to form laminar flow and carry out optical detection, the liquid path system replaces the injection pump and plunger pump in prior art to carry out sample loading process, simplifies pipeline connection, reduces the volume of liquid path system.Further, it is also convenient to clean sampling needle by cleaning passage opened on swab, avoid cross contamination between sample and negative influence on test result.

[0023] The utility model provides a kind of flow cytometer, the flow cytometer includes above-mentioned liquid path system, the flow cytometer has the whole function of above-mentioned liquid path system. DRAWINGS

[0024] In order to more clearly illustrate the technical scheme of the embodiment of the utility model, the following will be briefly introduced to the drawing needed to be used in embodiment, it should be understood that the following drawing only shows some embodiments of the utility model, therefore should not be regarded as the limitation to range, for ordinary skilled person in the art, under the premise of not paying creative labor, can also obtain other relevant drawings according to these drawings.

[0025] Figure 1 It is the schematic diagram of the liquid path system provided in the embodiment of the utility model;

[0026] Figure 2 It is the schematic diagram of the cleaning passage of swab provided in the embodiment of the utility model.

[0027] Figure: 100-negative pressure tank;111-first control valve;112-second control valve;113-third control valve;114-fourth control valve;115-fifth control valve;120-negative pressure tank body;121-pressure release port;122-first flow path channel;123-second flow path channel;130-damping device;140-first pressure sensor;200-sheath liquid pump;300-flow chamber;400-sampling needle;500-waste liquid pump;610-swab;611-cleaning passage;6111-first subchannel;6112-second subchannel;620-swab pump;700-sheath liquid bottle;800-deaerator;900-first filter;10-impulse removal device;20-cleaning liquid pump;30-cleaning liquid bottle;40-second pressure sensor;50-waste liquid bottle;60-sixth control valve;70-flow meter;80-check valve;90-air inlet filter;2000-sample. DETAILED DESCRIPTION

[0028] In order to make the purpose, technical scheme and advantages of the embodiments of the utility model clearer, the technical scheme in the embodiments of the utility model will be described clearly and completely in combination with the drawings in the embodiments of the utility model below. Obviously, the described embodiments are part of the embodiments of the utility model, rather than all the embodiments. The components of the embodiments of the utility model described and shown in the drawings can be arranged and designed in various different configurations.

[0029] Therefore, the following detailed description of the embodiments of the utility model provided in the drawings is not intended to limit the scope of the claimed utility model, but only represents selected embodiments of the utility model. Based on the embodiments in the utility model, all other embodiments obtained by those of ordinary skill in the art without creative labor fall within the scope of protection of the utility model.

[0030] It should be noted that: similar signs and letters represent similar items in the following drawings, therefore, once an item is defined in one drawing, it does not need to be further defined and explained in subsequent drawings.

[0031] In the description of the utility model, it should be explained that if the terms "upper", "lower", "inner", "outer" and the like indicate the orientation or positional relationship based on the orientation or positional relationship shown in the drawings, or the orientation or positional relationship commonly placed when the utility model product is used, it is only for the convenience of describing the utility model and simplifying the description, and does not indicate or imply that the indicated device or element must have a particular orientation, be constructed and operated in a particular orientation, therefore, it cannot be understood as a limitation on the utility model.

[0032] In addition, if the terms "first", "second" and the like appear, they are only used for differentiation description, and cannot be understood as indicating or implying relative importance.

[0033] It should be noted that the features in the embodiments of the utility model can be combined with each other without conflict.

[0034] As mentioned in the background, flow cytometry is usually used for analysis and statistics of cells or particles in samples. Flow cytometry mainly consists of a liquid system, an optical system, an electronic system and a data analysis system.

[0035] The liquid system, as one of the important components of the flow cytometer, transports the sample liquid containing cells or particles labeled by fluorescent dye and sheath liquid to the flow chamber, focuses the cells or particles by using fluid power principle, and makes the cells or particles pass through the flow chamber detection area one by one, so that the stable sample flow is the guarantee for subsequent signal detection, and is one of the core technologies of the whole instrument.

[0036] In the prior art, most flow cytometers have absolute counting function, and absolute volume counting is achieved by measuring the volume of the sample to be measured, and then dividing the number of measured cells by the volume of the sample to be measured, so as to obtain the concentration information of the sample to be measured, and achieve the purpose of absolute counting.

[0037] The absolute volume counting scheme is mainly realized by a quantitative pump capable of quantitatively delivering the sample volume, such as a syringe pump, a plunger pump, a peristaltic pump, etc., wherein the syringe pump and the plunger pump are used for sample loading, and this method has the problems of complex pipeline connection and large volume of liquid system.

[0038] Therefore, the liquid system and the flow cytometer provided in the embodiments of the present application can solve the above problems, which will be described in detail as follows. Figure 1 Figure 2 The liquid system and the flow cytometer provided in the embodiments of the present application can solve the above problems, which will be described in detail as follows.

[0039] The liquid system and the flow cytometer provided in the embodiments of the present application can solve the above problems, which will be described in detail as follows.

[0040] The liquid system and the flow cytometer provided in the embodiments of the present application can solve the above problems, which will be described in detail as follows. Figure 1 Figure 2 The liquid system and the flow cytometer provided in the embodiments of the present application can solve the above problems, which will be described in detail as follows.

[0041] In the working process, the negative pressure tank 100 is used to provide negative pressure for the flow chamber 300, and the sampling needle 400 is used to suck the sample into the flow chamber 300, and the sheath liquid bottle 700 is used to deliver the sheath liquid to the flow chamber 300, so that the sheath liquid wraps the sample 2000 to form a laminar flow for optical detection. The liquid system replaces the syringe pump and the plunger pump used in the prior art for sample loading, simplifies the pipeline connection, and reduces the volume of the liquid system. Furthermore, the cleaning channel 611 provided on the swab 610 also facilitates the cleaning operation of the sampling needle 400, avoids cross contamination between samples, and has a negative impact on the test results.

[0042] The liquid system and the flow cytometer provided in the embodiments of the present application can solve the above problems, which will be described in detail as follows. Figure 1 ​​The liquid path system further comprises a waste liquid bottle 50 and a waste liquid pump 500, the waste liquid pump 500 being connected between the waste liquid bottle 50 and the negative pressure tank 100, and the waste liquid pump 500 being used to perform a suction operation on the negative pressure tank 100 and cooperating with the negative pressure tank 100 to form a negative pressure source, so that stable and continuous sample loading measurement operation can be realized; the waste liquid bottle 50 is used to accommodate the waste liquid.

[0043] In addition, it should be noted that the negative pressure tank 100 can effectively reduce the pulsation generated by the waste liquid pump 500, improve the stability of the liquid flow, and improve the detection accuracy. At the same time, the pressure buffered by the negative pressure tank 100 can reduce the start-up time of sample loading, and improve the detection efficiency of the flow cytometer.

[0044] It should be noted that the sample loading time can be understood as the sum of the start-up time of sampling, the sampling time and the cleaning time after the sampling is completed. Among them, because the detection of the sample needs to be carried out in a stable state, the start-up time of sampling can be understood as the preparation time before starting to grab the detection data, including the preparation time of transporting the sample from the sample tube to the detection area and forming a stable laminar flow state.

[0045] It is easy to understand that the flow chamber 300 is used to accommodate the sample 2000 and the sheath liquid to be detected by cooperating with the optical module.

[0046] Next, the negative pressure tank 100 provided in the embodiment will be specifically introduced.

[0047] The negative pressure tank 100 comprises a negative pressure tank body 120 and a plurality of control valves, the control valves can be selected from solenoid valves, and the plurality of control valves are arranged on the negative pressure tank body 120. Specifically, in the embodiment, the number of control valves is five, and the five control valves are respectively a first control valve 111, a second control valve 112, a third control valve 113, a fourth control valve 114 and a fifth control valve 115.

[0048] Among them, the first control valve 111 is used to control the conduction or blockage between the waste liquid pump 500 and the flow chamber 300, so that the conduction between the waste liquid pump 500 and the flow chamber 300 is facilitated to realize the sample loading process when the first control valve 111 is opened.

[0049] It should be noted that in order to facilitate the negative pressure tank 100 to realize the function of rapid pressure relief, the negative pressure tank body 120 is provided with a first flow path channel 122 and a pressure relief port 121, one end of the first flow path channel 122 is communicated with the pressure relief port 121, and the other end of the first flow path channel 122 is used to communicate with the inside of the negative pressure tank body 120.

[0050] The third control valve 113 is arranged in the first flow path channel 122, and is used to control the conduction or blockage of the first flow path channel 122, so as to realize the conduction or blockage of the pressure relief port 121 and the negative pressure chamber in the negative pressure tank body 120. The negative pressure tank 100 further comprises an air inlet filter 90, and the pressure relief port 121 and the air inlet filter 90 are communicated.

[0051] In addition, in order to facilitate the monitoring of the pressure in the negative pressure tank 100, and at the same time, the pressure in the negative pressure tank 100 can be accurately controlled, the pressure fluctuation is reduced, the negative pressure tank 100 further comprises a first pressure sensor 140 and a damping device 130. The negative pressure tank body 120 is provided with a second flow path channel 123, one end of the second flow path channel 123 is communicated with the negative pressure tank body 120, the first pressure sensor 140 is communicated with the other end of the second flow path channel 123, and the first pressure sensor 140 can be communicated with the negative pressure chamber in the negative pressure tank body 120 through the second flow path channel 123. The first pressure sensor 140 facilitates the monitoring of the pressure in the negative pressure tank 100.

[0052] The damping device 130 is arranged in the negative pressure tank body 120, and the damping device 130 is communicated with the negative pressure tank body 120, that is, the damping device 130 is communicated with the negative pressure chamber in the negative pressure tank body 120.

[0053] The damping device 130 can be an air inlet damping pipe, or a throttle valve, a speed regulating valve or a filter, etc. The damping device 130 is communicated with the atmosphere, and can be used to adjust the output negative pressure of the waste liquid pump 500, and is equivalent to a pressure stabilizing device or a buffer, etc. The damping device 130 can accurately control the pressure in the negative pressure tank 100, and effectively reduces the pressure fluctuation.

[0054] In addition, it should be noted that the negative pressure tank 100 can realize the real-time emptying of the liquid in the negative pressure tank 100, and does not need to store the waste liquid, so that the liquid level sensor does not need to be arranged.

[0055] Therefore, the waste liquid pump 500 in the embodiment can select a diaphragm pump with low precision and low cost, and can accurately control the pressure in the negative pressure tank 100, so as to realize the effect of smaller output pressure fluctuation.

[0056] Please refer to Figure 2 and combine Figure 1 In order to further improve the cleaning effect, the liquid path system further comprises a swab pump 620, the swab pump 620 is communicated with one end of the cleaning channel 611, the other end of the cleaning channel 611 is communicated with the sheath liquid bottle 700, and the sheath liquid pump 200 is arranged on the pipeline between the sheath liquid bottle 700 and the other end of the cleaning channel 611.

[0057] The sampling needle 400 and the cleaning channel 611 can slide relative to each other to facilitate cleaning of the sampling needle 400. Here, the relative sliding of the sampling needle 400 and the cleaning channel 611 can be understood as the sampling needle 400 remaining stationary while the swab 610 moves downward, or the swab 610 remaining in the same position while the sampling needle 400 moves upward, which is equivalent to the retraction process of the sampling needle 400.

[0058] In this embodiment, the cleaning operation is preferably completed by keeping the sampling needle 400 stationary and moving the swab 610, thereby shortening the sampling channel as much as possible and reducing the fixed space occupied by sampling.

[0059] Among them, such as Figure 2 As shown, the cleaning channel 611 has a main channel and a first sub-channel 6111 and a second sub-channel 6112 connected to the main channel. The sampling needle 400 and the main channel of the cleaning channel 611 can slide relative to each other. The first sub-channel 6111 and the swab pump 620 are connected through a pipeline. The liquid circuit system also includes a sixth control valve 60 (a solenoid valve can be selected). The sixth control valve 60 is disposed in the pipeline between the first sub-channel 6111 and the swab pump 620. The second sub-channel 6112 is connected to the sheath fluid pump 200. The fourth control valve 114 is used to connect the sheath fluid pump 200 and the second sub-channel 6112 at the same time to control the opening or closing of the pipeline between the sheath fluid pump 200 and the second sub-channel 6112.

[0060] During the cleaning operation, the sheath fluid can be introduced into the main channel by activating the swab pump 620 and the sheath fluid pump 200. At the same time, the sampling needle 400 and the cleaning channel 611 slide relative to each other to clean the outer wall of the sampling needle 400. The waste liquid generated during cleaning flows into the waste liquid bottle 50 after passing through the sixth control valve 60 and the swab pump 620.

[0061] In addition, in this embodiment, the liquid system also includes a degasser 800 and a first pipeline. The sheath fluid bottle 700, sheath fluid pump 200, degasser 800 and flow chamber 300 are sequentially arranged in the first pipeline. The degasser 800 can remove air from the sheath fluid, solve the problem of air bubbles affecting the liquid flow, improve the stability of the liquid flow, improve the detection accuracy, and at the same time expand the adaptability of the flow cytometer to different usage environments.

[0062] Specifically, the liquid circuit system also includes a first filter 900 and an anti-pulsation device 10. The sheath fluid bottle 700, sheath fluid pump 200, anti-pulsation device 10, first filter 900, degasser 800 and flow chamber 300 are sequentially arranged in the first pipeline.

[0063] The fifth control valve 115 is used to connect the degasser 800 and the flow chamber 300 simultaneously. The fifth control valve 115 is also installed on the first pipeline. The fifth control valve 115 can control the opening or closing of the first pipeline.

[0064] The fourth control valve 114 is used to connect the first filter 900 and the second sub-channel 6112 at the same time, so as to control the pipeline conduction or blockage between the sheath liquid pump 200 and the second sub-channel 6112.

[0065] Meanwhile, the liquid system further comprises a second filter (not shown in the figure), which can be arranged on the first pipeline between the sheath liquid pump 200 and the sheath liquid bottle 700. The sheath liquid pump 200 can be a high-precision ceramic pump, and the second filter can protect the sheath liquid pump 200.

[0066] It should be noted that in some embodiments, the sheath liquid bottle 700, the sheath liquid pump 200, the first filter 900, the pulse elimination device 10, the degasser 800 and the flow chamber 300 can be arranged in sequence on the first pipeline.

[0067] In some embodiments, the liquid system can comprise a second filter, and does not comprise the first filter 900 and the pulse elimination device 10.

[0068] Alternatively, the liquid system comprises the first filter 900 and the pulse elimination device 10, and does not comprise the second filter. At this time, the sheath liquid pump 200 can be a peristaltic pump. In operation, the pulse elimination device 10 can eliminate the pulse generated by the sheath liquid pump 200, thereby ensuring the stability of the liquid system.

[0069] In order to facilitate the cleaning process of the liquid system, the liquid system further comprises a cleaning liquid pump 20 and a cleaning liquid bottle 30. One end of the cleaning liquid pump 20 is in communication with the cleaning liquid bottle 30, and the other end of the cleaning liquid pump 20 is in communication with the flow chamber 300.

[0070] The cleaning liquid pump 20 can deliver the cleaning liquid in the cleaning liquid bottle 30 to the flow chamber 300. Meanwhile, the cleaning liquid can enter the sample needle, flow through the flow meter 70 and the sampling needle 400, and clean the sampling channel and the flow chamber 300. One of the waste liquids can enter the waste liquid bottle 50 through the first control valve 111, the negative pressure tank 100 and the waste liquid pump 500, and the other waste liquid can enter the waste liquid bottle 50 through the sampling needle 400, the first sub-channel 6111 and the swab pump 620.

[0071] In addition, the liquid system further comprises a second pressure sensor 40 and a flow meter 70. The flow meter 70 is arranged on the pipeline between the sampling needle 400 and the flow chamber 300. Specifically, the flow meter 70 can be arranged on the pipeline between the sampling needle 400 and the sample needle. The second pressure sensor 40 is arranged on the pipeline between the flow chamber 300 and the sheath liquid pump 200.

[0072] In addition, the liquid path system further comprises a one-way valve 80, which is arranged in the third pipeline and is communicated between the cleaning channel 611 of the swab 610 and the pipeline between the swab pump 620 and the degasser 800. The one-way valve 80 can prevent backflow.

[0073] In addition, in the embodiment, the second control valve 112 is used to simultaneously connect the pipeline between the sixth control valve 60 and the swab pump 620 and the flow chamber 300, and control the pipeline between the pipeline between the sixth control valve 60 and the swab pump 620 and the flow chamber 300 to be conducted or disconnected. In this way, when the third control valve 113 and the first control valve 111 are opened, external air can enter the negative pressure tank 100, and the liquid in the flow chamber 300 can flow through the second control valve 112, the swab pump 620 and the waste liquid bottle 50 in sequence, so as to achieve the purpose of emptying the flow chamber 300.

[0074] In summary, the liquid path system and the flow cytometer provided by the embodiment have at least the following advantages:

[0075] 1. The negative pressure tank 100 is provided with negative pressure by the waste liquid pump 500, and the sample 2000 is loaded at a speed adjusted by the sheath liquid pump 200 controlling the sheath liquid supply amount. The flow rate of the sample 2000 is detected by the flow meter 70, so that the absolute volume counting can be realized while the sample is continuously loaded and measured. Compared with the injection pump and the plunger pump loading mode, the pipeline connection is simple, and the volume of the liquid path system is greatly reduced.

[0076] 2. The negative pressure tank 100 and the waste liquid pump 500 cooperate to form a negative pressure source. The negative pressure tank 100 can realize rapid pressure relief. In addition, the negative pressure tank 100 has a damping device 130, which is equivalent to a pressure stabilizing device or a buffer. The damping device 130 can accurately control the pressure in the negative pressure tank 100 and effectively reduce the pressure fluctuation. In addition, the negative pressure tank 100 can realize real-time emptying of the liquid in the negative pressure tank 100, and does not need to store waste liquid, so that a liquid level sensor does not need to be arranged. In addition, the waste liquid pump 500 can be a diaphragm pump with low precision and low cost, which can also accurately control the pressure in the negative pressure tank 100 and realize smaller output pressure fluctuation.

[0077] 3. The de-pulsation device 10 can effectively remove the liquid flow pulsation generated by the sheath liquid pump 200, improve the stability of the liquid flow, and improve the detection accuracy.

[0078] 4. The cooperation of the swab 610, the sheath liquid bottle 700, the sheath liquid pump 200 and other components can effectively clean the flow chamber 300 and the sampling needle 400, improve the consistency of the detection result, reduce the pollution rate, and make the flow cytometer more complete and automatic.

[0079] 5、The negative pressure tank 100 can effectively reduce the pulsation generated by the waste liquid pump 500, improve the stability of the liquid flow, improve the detection accuracy, and the pressure buffered by the negative pressure tank 100 can reduce the starting time of the beginning collection, and improve the detection efficiency of the flow cytometer.

[0080] 6、The air content in the sheath liquid is removed by the air remover 800, the influence of the bubbles on the liquid flow is solved, the stability of the liquid flow is improved, the detection accuracy is improved, and the adaptability of the flow cytometer to different use environments is improved.

[0081] The above is only a specific implementation manner of the present application, but the protection scope of the present application is not limited to this, any person skilled in the art can easily think of changes or replacements within the technical range disclosed by the present application, which should be covered in the protection scope of the present application. Therefore, the protection scope of the present application should be subject to the protection scope of the claims.

Claims

1. A fluidic system suitable for flow cytometers, characterized in that, The fluid system includes: a negative pressure tank (100), a flow chamber (300), a sampling needle (400), a swab (610), and a sheath fluid bottle (700); The negative pressure tank (100), the sampling needle (400) and the sheath fluid bottle (700) are all connected to the flow chamber (300), the swab (610) is provided with a cleaning channel (611), and the sampling needle (400) is disposed through the cleaning channel (611).

2. The fluid circuit system according to claim 1, characterized in that, The negative pressure tank (100) includes a negative pressure tank body (120) and a control valve. The negative pressure tank body (120) is provided with a first flow path channel (122) and a pressure relief port (121). One end of the first flow path channel (122) is connected to the pressure relief port (121), and the other end of the first flow path channel (122) is used to connect to the inside of the negative pressure tank body (120). The control valve is located in the first flow path channel (122).

3. The fluid circuit system according to claim 1, characterized in that, The negative pressure tank (100) includes a negative pressure tank body (120), a first pressure sensor (140) and / or a damping device (130). The first pressure sensor (140) is used to output pressure parameters describing the negative pressure tank body (120). The damping device (130) is disposed in the negative pressure tank body (120) and is internally connected to the negative pressure tank body (120).

4. The fluid circuit system according to claim 1, characterized in that, The fluid system also includes a swab pump (620), one end of the cleaning channel (611) is connected to the swab pump (620), and the other end of the cleaning channel (611) is connected to the sheath fluid bottle (700); And / or, the liquid system further includes a waste liquid bottle (50) and a waste liquid pump (500), the waste liquid pump (500) being connected between the waste liquid bottle (50) and the negative pressure tank (100).

5. The fluid circuit system according to claim 4, characterized in that, The sampling needle (400) and the cleaning channel (611) can slide relative to each other.

6. The fluid circuit system according to claim 4, characterized in that, The fluid system further includes a sheath fluid pump (200), a degasser (800), and a first pipeline, wherein the sheath fluid bottle (700), the sheath fluid pump (200), the degasser (800), and the flow chamber (300) are sequentially arranged in the first pipeline.

7. The fluid circuit system according to claim 6, characterized in that, The fluid system further includes a first filter (900), which is disposed in the first pipeline; And / or, the fluid system further includes a de-pulsation device (10) disposed in the first pipeline; And / or, the fluid system further includes a second filter disposed in the first line between the sheath fluid pump (200) and the sheath fluid bottle (700).

8. The fluid circuit system according to claim 4, characterized in that, The liquid circuit system also includes a cleaning fluid pump (20) and a cleaning fluid bottle (30), one end of the cleaning fluid pump (20) is connected to the cleaning fluid bottle (30), and the other end of the cleaning fluid pump (20) is connected to the flow chamber (300).

9. The fluid circuit system according to claim 1, characterized in that, The liquid circuit system also includes a flow meter (70), which is disposed on the pipeline between the sampling needle (400) and the flow chamber (300); And / or, the fluid system further includes a second pressure sensor (40) disposed on the pipeline between the flow chamber (300) and the sheath fluid bottle (700).

10. A flow cytometer, characterized in that, Includes the fluid circuit system as described in any one of claims 1-9.

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