Culture device for immunohistochemistry of cell climbing slide

Abstract

The utility model relates to the technical field of cell climbing slide immunohistochemistry, and discloses a culture device for cell climbing slide immunohistochemistry, which comprises a culture plate monomer, a fixing component is arranged in the culture plate monomer, the top of the culture plate monomer is rotatably connected with a cover plate, the front side of the culture plate monomer is fixedly connected with a splicing block, and the splicing block is fixedly connected with the culture plate monomer. The tops of the culture plate single bodies are fixedly connected with a fixing shell, a splicing assembly is arranged in the fixing shell, and a clamping groove is formed in the cover plate; the fixing assembly comprises a connecting column, the connecting column is fixedly connected to the interior of the culture plate single body, the outer side of the connecting column is slidably connected with a sliding plate, and the periphery of the connecting column is sleeved with a first spring. According to the utility model, under the matching of the connecting rod, the spring I and the sliding plate, the clamping block is controlled to be clamped into the clamping groove, the position of the cover plate is fixed, only the cover plate of a single culture tank is opened, and other culture tanks are prevented from being polluted.

Description

TECHNICAL FIELD

[0001] The utility model relates to cell climbing slice immunohistochemical technical field especially relates to a kind of culture devices for cell climbing slice immunohistochemistry. BACKGROUND

[0002] Cell climbing slice immunohistochemistry is an immunohistochemical technique carried out at cell level, for detecting the expression and localization of specific antigens in cells, widely used in tumor research, cell biology research and drug development fields.

[0003] Common cell climbing slice immunohistochemistry culture device is cell culture plate, advantage lies in that the culture of multiple samples can be carried out simultaneously, which is convenient for control experiment and high-throughput experiment.

[0004] Although the existing cell climbing slice immunohistochemistry culture device can ensure the airtightness of cell culture plate, when only single culture tank is operated during long-term culture of cells, the entire upper cover needs to be opened, so that all cell climbing slices are exposed to the outside, which is easy to contaminate the culture in other culture tanks, therefore, a cell climbing slice immunohistochemistry culture device is proposed to solve the above problems. UTILITY MODEL CONTENTS

[0005] In order to make up for the above shortcomings, the utility model provides a kind of cell climbing slice immunohistochemistry culture device, to improve the problem of opening the whole cover plate when only single culture tank is operated in prior art, increase the risk of contaminating other culture tanks.

[0006] In order to achieve the above purpose, the utility model adopts the following technical scheme:

[0007] A kind of cell climbing slice immunohistochemistry culture device, including culture plate monomer, the inside of the culture plate monomer is provided with fixed component, the top of the culture plate monomer is rotatably connected with cover plate, the front side of the culture plate monomer is fixedly connected with splicing block, the top of the culture plate monomer is fixedly connected with fixed shell, the inside of the fixed shell is provided with splicing component, the inside of the cover plate is equipped with clamping groove;The fixed component includes connecting column, the connecting column is fixedly connected in the inside of the culture plate monomer, the outer side of the connecting column is slidably connected with sliding plate, spring one is sleeved on the outer periphery of the connecting column, the side of the sliding plate is fixedly connected with clamping block;

[0008] As a further description of the above technical scheme:

[0009] One end of the sliding plate is slidably connected in the inside of the cover plate, the clamping block is mutually clamped with the clamping groove;

[0010] As a further description of the above technical scheme:

[0011] The splicing assembly comprises a sliding insertion rod which is slidingly connected in the interior of the fixed shell, the outer periphery of the sliding insertion rod is provided with spring two, and the top of the sliding insertion rod is rotationally connected with a rotating block;

[0012] As further description of the above technical solution:

[0013] The interior of the culture plate monomer is provided with an empty groove, the empty groove is arranged below the sliding insertion rod, and the splicing block is slidingly connected in the interior of the empty groove;

[0014] As further description of the above technical solution:

[0015] The top of the splicing block is provided with a splicing groove, and the sliding insertion rod and the splicing groove are mutually clamped;

[0016] As further description of the above technical solution:

[0017] The bottom of the cover plate is fixedly connected with a sealing ring, the top of the culture plate monomer is provided with a sealing groove, and the sealing ring is arranged in the interior of the sealing groove;

[0018] As further description of the above technical solution:

[0019] The interior of the culture plate monomer is provided with a culture groove;

[0020] As further description of the above technical solution:

[0021] The top of the cover plate is fixedly connected with a handle.

[0022] The utility model has the advantages of the following:

[0023] 1、 the utility model discloses, spring one extrusion connects the baffle on the column, to its outwardly moving pressure is added, make baffle take card block outwardly moving and enter the card slot, the position of fixed cover plate is realized, when operating to single culture groove, only need to open the culture groove of needing operating, avoid making the culture in other culture grooves be contaminated.

[0024] 2、 the utility model discloses, rotate rotating block and put flat, make sliding insertion rod move down, through the cooperation of spring two, sliding insertion rod inserts the splicing groove of splicing block, realize quick splicing multiple culture plate monomers, can decide the culture plate monomer quantity of splicing according to the quantity of needing culture, avoid causing the waste of resources, improve practicality. DRAWINGS

[0025] Figure 1 A kind of cell crawling piece immunohistochemical culture device is provided for the utility model, and its three-dimensional schematic view is shown in the figure;

[0026] Figure 2The utility model provides a kind of cell culture device for the structure diagram of culture tank of cell climbing slice immunohistochemistry;

[0027] Figure 3 The utility model provides a kind of cell culture device for the structure diagram of sliding plate of cell climbing slice immunohistochemistry;

[0028] Figure 4 The utility model provides a kind of cell culture device for the structure diagram of fixed shell of cell climbing slice immunohistochemistry;

[0029] Figure 5 The utility model provides a kind of cell culture device for the structure diagram of sliding insertion rod of cell climbing slice immunohistochemistry.

[0030] Legend:

[0031] 1, culture plate monomer;2, fixed assembly;3, cover plate;4, fixed shell;5, splicing assembly;6, connecting column;7, sliding plate;8, spring one;9, clamping block;10, sliding insertion rod;11, spring two;12, rotating block;13, splicing block;14, splicing groove;15, empty slot;16, sealing ring;17, sealing groove;18, culture tank;19, clamping groove;20, handle. Specific implementation

[0032] The technical scheme in the embodiments of the utility model will be described clearly and completely below in conjunction with the drawings in the embodiments of the utility model. Obviously, the described embodiments are only part of the embodiments of the utility model, not all the embodiments. Based on the embodiments in the utility model, all other embodiments obtained by those skilled in the art without creative labor belong to the scope of protection of the utility model.

[0033] Reference Figures 1-4 An embodiment provided by the utility model: a cell culture device for cell climbing slice immunohistochemistry, which comprises a culture plate monomer 1, the inside of the culture plate monomer 1 is provided with a fixed assembly 2, the top of the culture plate monomer 1 is rotatably connected with a cover plate 3, the front side of the culture plate monomer 1 is fixedly connected with a splicing block 13, the top of the culture plate monomer 1 is fixedly connected with a fixed shell 4, the inside of the fixed shell 4 is provided with a splicing assembly 5, and the inside of the cover plate 3 is provided with a clamping groove 19;The fixed assembly 2 comprises a connecting column 6, the connecting column 6 is fixedly connected to the inside of the culture plate monomer 1, and the connecting column 6 is used to connect the culture plate monomer 1 and the fixed assembly 2.The outside of the connecting column 6 is slidably connected with a sliding plate 7, the outer periphery of the connecting column 6 is sleeved with a spring one 8, the side surface of the sliding plate 7 is fixedly connected with a clamping block 9, the sliding plate 7 is extruded by the spring one 8, so that the clamping block 9 moves outward. One end of the sliding plate 7 is slidably connected to the inside of the cover plate 3, the clamping block 9 is clamped with the clamping groove 19, the clamping block 9 is moved outward to make it clamped into the clamping groove 19.

[0034] Referring to Figure 1 , Figure 4 and Figure 5 , the inside of the fixed shell 4 is provided with a spring 11 around the sliding plug 10, and the top of the sliding plug 10 is rotatably connected with a rotating block 12. Rotating the rotating block 12 makes it lie flat, and the sliding plug 10 moves downward. The inside of the culture plate monomer 1 is provided with a hollow groove 15, which is arranged below the sliding plug 10. The splicing block 13 is slidably connected inside the hollow groove 15. By providing the hollow groove 15, the splicing block 13 enters the space below the sliding plug 10. The top of the splicing block 13 is provided with a splicing groove 14, and the sliding plug 10 is clamped with the splicing groove 14. When the sliding plug 10 moves downward, it is clamped into the splicing groove 14 through the cooperation of the spring 11, and the two culture plate monomers 1 are quickly spliced.

[0035] Referring to Figure 1 , Figure 2 and Figure 4 , the bottom of the cover plate 3 is fixedly connected with a sealing ring 16, and the top of the culture plate monomer 1 is provided with a sealing groove 17. The sealing ring 16 is arranged inside the sealing groove 17, and the sealing ring 16 is used to keep the culture plate monomer 1 inside sealed. The inside of the culture plate monomer 1 is provided with a culture groove 18, which provides space for placing cell climbing sheets. The top of the cover plate 3 is fixedly connected with a handle 20, which is used to assist in opening the cover plate 3.

[0036] Working principle: take out the corresponding number of culture plate monomers 1 needed for culture, rotate the rotating block 12 to make it stand, move the sliding plug 10 upward, align the splicing block 13 on the front side of one of the culture plate monomers 1 with the hollow groove 15 of the other culture plate monomer 1, and insert it into the hollow groove 15. After docking, rotate the rotating block 12 again to make it lie flat, and the sliding plug 10 moves downward. Under the cooperation of the spring 11, it is inserted into the splicing groove 14, and the work of quickly splicing the culture plate monomer 1 is completed. When it is necessary to operate the cell climbing sheet inside a single culture plate monomer 1, press the sliding plate 7 on the top of the culture plate monomer 1 where the cell climbing sheet is located inward, extrude the spring 8 on the connecting column 6, and make the clamping block 9 separate from the clamping groove 19 of the cover plate 3. At this time, the cover plate 3 of the culture plate monomer 1 can be opened through the handle 20, and the cell climbing sheet can be operated. After the operation is completed, press the sliding plate 7 inward again, put down the cover plate 3, and then release the sliding plate 7. The pressure exerted on the sliding plate 7 by the spring 8 makes it move outward with the clamping block 9, and the clamping block 9 is clamped into the clamping groove 19, fixing the position of the cover plate 3. Through the cooperation of the sealing ring 16 and the sealing groove 17, the airtightness of the culture groove 18 is guaranteed.

[0037] Finally, it should be noted that: the above only for the preferred embodiments of the present application, and is not intended to limit the present application, although the foregoing embodiments of the present application has been described in detail, for the skilled in the art, it still can be modified, or for part of the technical features of the equivalent replacement, the spirit and principles of the present application, made any modification, equivalent replacement, improvement, etc., should be included within the scope of the present application.

Claims

1. A culture device for cell climbing slice immunohistochemistry, comprising a culture plate monomer (1), characterized in that: The inside of the culture plate monomer (1) is provided with a fixing assembly (2), the top of the culture plate monomer (1) is rotatably connected with a cover plate (3), the front side of the culture plate monomer (1) is fixedly connected with a splicing block (13), the top of the culture plate monomer (1) is fixedly connected with a fixed shell (4), the inside of the fixed shell (4) is provided with a splicing assembly (5), and the inside of the cover plate (3) is provided with a clamping groove (19). The fixing assembly (2) comprises a connecting column (6) fixedly connected to the inside of the culture plate monomer (1), a sliding plate (7) slidably connected to the outside of the connecting column (6), a spring (8) sleeved on the outer periphery of the connecting column (6), and a clamping block (9) fixedly connected to the side of the sliding plate (7). The splicing assembly (5) comprises a sliding insertion rod (10) slidably connected to the inside of the fixed shell (4), a spring (11) sleeved on the outer periphery of the sliding insertion rod (10), and a rotating block (12) rotatably connected to the top of the sliding insertion rod (10). The inside of the culture plate monomer (1) is provided with an empty groove (15), the empty groove (15) is arranged below the sliding insertion rod (10), and the splicing block (13) is slidably connected to the inside of the empty groove (15). The top of the splicing block (13) is provided with a splicing groove (14), and the sliding insertion rod (10) and the splicing groove (14) are mutually clamped. 2.The culture device for cell climbing slice immunohistochemistry according to claim 1, characterized in that: One end of the sliding plate (7) is slidably connected to the inside of the cover plate (3), and the clamping block (9) and the clamping groove (19) are mutually clamped. 3.The culture device for cell climbing slice immunohistochemistry according to claim 1, characterized in that: The bottom of the cover plate (3) is fixedly connected with a sealing ring (16), the top of the culture plate monomer (1) is provided with a sealing groove (17), and the sealing ring (16) is arranged in the sealing groove (17). 4.The culture device for cell climbing slice immunohistochemistry according to claim 1, characterized in that: The inside of the culture plate monomer (1) is provided with a culture groove (18). The inside of the culture plate monomer (1) is provided with a culture groove (18).

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