Chemiluminescence imager
By designing a darkroom, a light-emitting device, and a data acquisition device in a chemiluminescence imager, and combining them with a CCD camera, a reflector, and a supplementary lighting device, the problem of weak signal detection in existing technologies has been solved, and high-sensitivity and high-resolution signal acquisition and analysis have been achieved.
Patent Information
- Application Number
- CN202422225798.1
- Authority / Receiving Office
- CN · China
- Patent Type
- Utility models(China)
- Current Assignee / Owner
- Filing Date
- 2024-09-11
- Publication Date
- 2025-12-02
- Estimated Expiration
- 2034-09-11
AI Technical Summary
Existing chemiluminescence imagers have difficulty detecting weak chemiluminescence signals with high sensitivity.
A chemiluminescence imager was designed, including a dark chamber, a light-emitting device, and a data acquisition device. The light-emitting device is located at the bottom of the dark chamber, and the light is collected by the data acquisition device through the light channel at the top of the dark chamber. A CCD camera is used for high-sensitivity data acquisition, and the signal acquisition quality is improved by a reflection device, a supplementary lighting device, and a heat dissipation device.
It achieves high sensitivity and high resolution detection of chemiluminescence signals, enabling clear acquisition and analysis of weak chemiluminescence signals, and providing high-quality images and quantitative analysis capabilities.
Smart Images
Figure CN223624124U_ABST
Abstract
Description
Technical Field
[0001] This application relates to the field of chemiluminescence, and in particular to a chemiluminescence imager. Background Technology
[0002] Chemiluminescence imaging instruments are mainly used to detect samples based on chemiluminescence reactions, such as the blot membranes used in experiments like Western Blot, Northern Blot, and Southern Blot. Utility Model Content
[0003] Purpose of the utility model: This utility model provides a chemiluminescence imager that can detect weak chemiluminescence signals and has high sensitivity.
[0004] Technical solution: This utility model embodiment provides a chemiluminescence imaging system. The chemiluminescence imager has a first direction and a second direction, and includes a darkroom, a light-emitting device, and a collection device. The light-emitting device is disposed at the bottom of the darkroom, and a light channel is disposed at the top of the darkroom. The light emitted by the light-emitting device passes through the light channel disposed on the darkroom and is collected by the collection device.
[0005] In some embodiments, the darkroom includes a first panel, a second panel disposed opposite to the first panel, a third panel and a fourth panel disposed between the first panel and the second panel, the first panel, the second panel, the third panel and the fourth panel forming a darkroom cavity, the second panel having a first through hole, the first through hole penetrating the second panel to form the light channel.
[0006] In some embodiments, the acquisition device includes a camera and a lens disposed at the front end of the camera.
[0007] In some embodiments, the camera is a CCD camera.
[0008] In some embodiments, the chemiluminescence imager includes a reflective device, wherein a first light beam emitted by the reflective device extends along the second direction; the chemiluminescence imager includes a reflective device, wherein the first light beam is reflected by the reflective device and enters the acquisition device.
[0009] In some embodiments, the reflecting device is located on the transmission path of the first light beam, and the reflecting device is tilted relative to the first direction.
[0010] In some embodiments, the reflecting device is an aluminum-coated reflector.
[0011] In some embodiments, the light-emitting device includes a driving mechanism, a lead screw connected to the output end of the driving mechanism, and a reaction chamber connected to the lead screw.
[0012] In some embodiments, a base is provided below the reaction chamber, and the base is connected to the lead screw; the light-emitting device further includes a slide rail extending along the first direction, the base is mounted on the slide rail, and the base moves along the slide rail in the first direction under the drive of the lead screw.
[0013] In some embodiments, the chemiluminescence imager includes a supplementary lighting device located between the light-emitting device and the reflective device, the supplementary lighting device being arranged around the first through-hole.
[0014] In some embodiments, the supplementary lighting device includes a reflective mechanism, a supplementary light, and a light guide mechanism; the reflective mechanism is arranged around the first through hole, the supplementary light is arranged above the reflective mechanism, and the light guide mechanism is located inside the reflective mechanism.
[0015] In some embodiments, the inner wall of the light guide mechanism contracts inward from bottom to top.
[0016] In some embodiments, the light guiding mechanism includes a first light guide plate, a second light guide plate, a third light guide plate, and a fourth light guide plate, wherein the first light guide plate, the second light guide plate, the third light guide plate, and the fourth light guide plate form a frame structure.
[0017] In some embodiments, the inner walls of the first light guide plate, the second light guide plate, the third light guide plate, and the fourth light guide plate contract inward from bottom to top.
[0018] In some embodiments, a second through hole is provided at the connection point of the first light guide plate, the second light guide plate, the third light guide plate and the fourth light guide plate.
[0019] In some embodiments, the inner walls of the first light guide plate, the second light guide plate, the third light guide plate, and the fourth light guide plate have a frosted structure.
[0020] In some embodiments, the second through hole is located near the top of the light guide mechanism.
[0021] In some embodiments, the chemiluminescence imager includes a heat dissipation device for dissipating heat from the acquisition device.
[0022] In some embodiments, the chemiluminescence imager includes a display device connected to the acquisition device.
[0023] Beneficial effects: The chemiluminescence imager of this application includes a darkroom, a light-emitting device, and a data acquisition device; the light-emitting device is located at the bottom of the darkroom, and a light channel is provided at the top of the darkroom. The light emitted by the light-emitting device passes through the light channel provided on the darkroom and is acquired by the data acquisition device. The chemiluminescence imager of this application has high sensitivity and high resolution. Attached Figure Description
[0024] Figure 1 This is a schematic diagram of the chemiluminescence imager in the embodiments of this application;
[0025] Figure 2 for Figure 1 Schematic diagram of the cross-sectional structure from the middle AA plane;
[0026] Figure 3 This is a schematic diagram of the structure of the light-emitting device in the embodiments of this application;
[0027] Figure 4 This is a partial structural diagram of the chemiluminescence imager according to an embodiment of this application;
[0028] Figure 5 for Figure 4 Schematic diagram of the structure of section A;
[0029] Figure 6 This is a schematic diagram of the light guide mechanism structure in the embodiments of this application;
[0030] Figure 7 This is a front view of the light guide mechanism in an embodiment of this application;
[0031] Figure 8 This is a schematic diagram of the chemiluminescence imaging device in the embodiments of this application. Detailed Implementation
[0032] The chemiluminescence imager in the embodiments of this application will be further described below with reference to the accompanying drawings.
[0033] like Figure 1 and Figure 2 As shown, this application provides a chemiluminescence imager with a first direction X and a second direction Y. The first direction X and the second direction Y are arranged perpendicularly to each other or approximately perpendicularly.
[0034] In this embodiment of the application, the chemiluminescence imaging device includes a darkroom 100, a light-emitting device 200, and a data acquisition device 300.
[0035] In some embodiments, the darkroom 100 includes a first panel 101, a second panel 102 opposite to the first panel 101, a third panel 103 and a fourth panel 104 disposed between the first panel 101 and the second panel 102. In some embodiments, the third panel 103 and the fourth panel 104 are arranged parallel to each other and perpendicular to the first panel 101 and the second panel 102. The first panel 101, the second panel 102, the third panel 103 and the fourth panel 104, together with the front and rear panels, form a darkroom cavity 120. The second panel 102 is provided with a first through hole 1021, which penetrates the second panel 102 to form a light channel 110 for the light beam emitted by the light-emitting device 200 to pass through the light channel 110. In this embodiment, the darkroom 100 is used to shield external light and ensure image quality.
[0036] In some embodiments, the light-emitting device 200 is disposed at the bottom of the darkroom 100, which surrounds the outer periphery of the light-emitting device 200 to shield external light. The light beam emitted by the light-emitting device 200 passes through a light channel 110 disposed at the top of the darkroom 100 and is collected by the collecting device 300. Figure 3 and Figure 8 As shown, in some embodiments, the light-emitting device 200 includes a driving mechanism 201, a lead screw 202 connected to the output end of the driving mechanism 201, and a reaction chamber 203 connected to the lead screw 202. A base 204 is disposed below the reaction chamber 203, and the base 204 is connected to the lead screw 202. In some embodiments, the light-emitting device 200 includes a slide rail 205 extending along a first direction X. The base 204 is mounted on the slide rail 205, and the base 204 moves along the slide rail 205 in the first direction X under the drive of the lead screw 202. In some specific embodiments, the driving mechanism 201 is a motor. The power provided by the driving mechanism 201 can push out the reaction chamber 203 for adding samples into the reaction chamber 203. After the samples are added, the reaction chamber 203 is sent into the dark chamber 100 under the drive of the driving mechanism 201.
[0037] In some embodiments, different substances may be placed in the reaction chamber 203 according to its purpose, typically an enzymatic reaction system. For example, in a Western blotting experiment, horseradish peroxidase (HRP) in the reaction chamber 203 can catalyze the luminol substrate to emit light, thereby causing the antibody bound to the target protein to emit visible light. In some embodiments, the first light beam L1 emitted by the luminescent device 200 extends along the second direction Y, and the first light beam L1 enters the collection device 300 after passing through the reflection device 400.
[0038] like Figure 1 and Figure 4As shown, in some embodiments, the acquisition device 300 includes a camera 301 and a lens 302 disposed at the front end of the camera 301. For example, if the camera is a CCD camera, a high-sensitivity cooled CCD camera acquires chemiluminescence signals, converts the light signals into electrical signals, and digitizes and stores them as an image. This application improves image clarity by focusing light through the lens 302 at the front end of the CCD camera.
[0039] In some embodiments, the reflecting device 400 is disposed on the transmission path of the light channel 110. In some specific embodiments, the reflecting device 400 is an aluminum-coated film reflector. The reflecting device 400 forms a 45° angle with the first direction X, and the reflecting device 400 is located on the extension line of the central axis O1 of the collecting device 300. By setting the reflecting device 400, this application separates the light-emitting device 200 disposed in the darkroom 100 from the collecting device 300, so that the two do not interfere with each other, and ensures the shielding effect of the darkroom 100 on light.
[0040] like Figure 5 , Figure 6 and Figure 7 As shown, in some embodiments, the chemiluminescence imager of this application further includes a supplementary lighting device 500, which is located between the light-emitting device 200 and the reflective device 400. In some specific embodiments, the supplementary lighting device 500 is located at the upper end of the darkroom 100 and is arranged around the first through hole 1021. The supplementary lighting device 500 provides a shadowless light source for the acquisition device 300 of the chemiluminescence imager to take pictures.
[0041] In some embodiments, the supplementary lighting device 500 includes a reflective mechanism 501, a supplementary light 502, and a light guide mechanism 503. The reflective mechanism 501 is disposed around the first through hole 1021. In some specific embodiments, the reflective mechanism 501 is reflective paper. The supplementary light 502 is disposed above the reflective mechanism 501, and the reflective mechanism 501 surrounds the supplementary light 502. In some specific embodiments, the supplementary light 502 is an LED ring-shaped shadowless lamp.
[0042] In some embodiments, the light guide mechanism 503 in the supplementary lighting device 500 of this application is located inside the reflection mechanism 501, and the light guide mechanism 503 as a whole presents an inwardly converging structure. In some embodiments, the inner wall of the light guide mechanism 503 contracts inward from bottom to top to form an inwardly converging structure. In some embodiments, the light guide mechanism 503 includes a first light guide plate 5031, a second light guide plate 5032, a third light guide plate 5033, and a fourth light guide plate 5034, the first light guide plate 5031, the second light guide plate 5032, the third light guide plate 5033, and the fourth light guide plate 5034 forming a frame-like structure, and the inner walls of the first light guide plate 5031, the second light guide plate 5032, the third light guide plate 5033, and the fourth light guide plate 5034 contract inward from bottom to top.
[0043] In some specific embodiments, the inner walls of the first light guide plate 5031, the second light guide plate 5032, the third light guide plate 5033, and the fourth light guide plate 5034 have a frosted structure.
[0044] In some embodiments, to make the light emitted by the supplementary light 502 more uniform, a second through hole 5035 is provided at the connection point of the first light guide plate 5031, the second light guide plate 5032, the third light guide plate 5033, and the fourth light guide plate 5034. The second through hole 5035 can further disperse the light beam emitted by the supplementary light 502. In some specific embodiments, the second through hole 5035 is located near the top of the light guide mechanism 503. The second through hole 5035 located at the top can better improve the uniformity of light.
[0045] In some specific embodiments, the downward illumination of the LED ring-shaped shadowless lamp is reflected by reflective paper arranged around the perimeter, converging all the outwardly scattered light inward. A frosted light guide plate with an inclined angle, located inside the reflective paper, further refracts the light and homogenizes it. In this embodiment, the supplementary lighting device 500 ensures that objects within the light field of the ring-shaped shadowless light source receive 360° illumination without blind spots and without shadows.
[0046] In some embodiments, the chemiluminescence imager in this application further includes a heat dissipation device 600. In some specific embodiments, the heat dissipation device 600 is a cooling fan. The heat dissipation device 600 is used to dissipate heat from the acquisition device 300 to avoid the ambient temperature affecting the image quality acquired by the acquisition device 300.
[0047] In some embodiments, the chemiluminescence imager in this application further includes a display device 700. In some specific embodiments, the display device 700 is a display screen, which is connected to the acquisition device 300. The acquisition device 300 converts the light signal into an electrical signal and digitizes and stores it as an image, which is then transmitted to the display screen.
[0048] In some embodiments, the chemiluminescence imager further includes a filter located in the transmission path of the first beam L1 to select light of a specific wavelength and reduce background noise.
[0049] The working method of the chemiluminescence imager of this application is as follows: the sample containing the chemiluminescent reactant is placed in a dark box, the exposure time and other shooting parameters are set, the CCD camera starts the exposure, and the chemiluminescence signal is acquired. The CCD camera converts the light signal into an electrical signal and digitizes and stores it as an image. The image is analyzed using image processing software, such as quantitative analysis and background subtraction.
[0050] The chemiluminescence imager of this application features an integrated design, is simple and easy to operate, can detect weak chemiluminescence signals, has high resolution, and can obtain clear images for easy observation and analysis. This chemiluminescence imager can also perform quantitative analysis, obtaining more accurate results by quantitatively analyzing the chemiluminescence signals.
Claims
1. A chemiluminescence imaging device, characterized in that, The chemiluminescence imager has a first direction (X) and a second direction (Y), and includes a dark chamber (100), a light-emitting device (200), and a collection device (300). The light-emitting device (200) is located at the bottom of the dark chamber (100), and a light channel (110) is provided at the top of the dark chamber (100). The light emitted by the light-emitting device (200) is collected by the collection device (300) through the light channel (110) provided on the dark chamber (100).
2. The chemiluminescence imager according to claim 1, characterized in that, The darkroom (100) includes a first panel (101), a second panel (102) disposed opposite to the first panel (101), a third panel (103) and a fourth panel (104) disposed between the first panel (101) and the second panel (102). The first panel (101), the second panel (102), the third panel (103) and the fourth panel (104) form a darkroom cavity (120). The second panel (102) is provided with a first through hole (1021), which penetrates the second panel (102) to form the light channel (110).
3. The chemiluminescence imager according to claim 1, characterized in that, The acquisition device (300) includes a camera (301) and a lens (302) disposed at the front end of the camera (301); and / or, The camera (301) is a CCD camera.
4. The chemiluminescence imager according to claim 2, characterized in that, The chemiluminescence imager includes a reflector (400), and a first light beam (L1) emitted by the light-emitting device (200) extends along the second direction (Y). The first light beam (L1) enters the acquisition device (300) after being reflected by the reflector (400).
5. The chemiluminescence imager according to claim 4, characterized in that, The reflecting device (400) is located on the transmission path of the first light beam (L1), and the reflecting device (400) is tilted relative to the first direction (X); and / or, The reflecting device (400) is an aluminum-coated reflector.
6. The chemiluminescence imager according to claim 1, characterized in that, The light-emitting device (200) includes a driving mechanism (201), a lead screw (202) connected to the output end of the driving mechanism (201), and a reaction chamber (203) connected to the lead screw (202).
7. The chemiluminescence imager according to claim 6, characterized in that, A base (204) is provided below the reaction chamber (203), and the base (204) is connected to the lead screw (202); the light-emitting device (200) also includes a slide rail (205) extending along the first direction (X), the base (204) is mounted on the slide rail (205), and the base (204) moves along the slide rail (205) in the first direction (X) under the drive of the lead screw (202).
8. The chemiluminescence imager according to claim 4, characterized in that, The chemiluminescence imager includes a supplementary lighting device (500), which is located between the light-emitting device (200) and the reflective device (400), and is arranged around the first through hole (1021).
9. The chemiluminescence imager according to claim 8, characterized in that, The supplementary lighting device (500) includes a reflective mechanism (501), a supplementary light (502), and a light guide mechanism (503); the reflective mechanism (501) is arranged around the first through hole (1021), the supplementary light (502) is arranged above the reflective mechanism (501), and the light guide mechanism (503) is located inside the reflective mechanism (501); and / or, The inner wall of the light guide mechanism (503) contracts inward from bottom to top.
10. The chemiluminescence imager according to claim 9, characterized in that, The light guiding mechanism (503) includes a first light guide plate (5031), a second light guide plate (5032), a third light guide plate (5033), and a fourth light guide plate (5034), wherein the first light guide plate (5031), the second light guide plate (5032), the third light guide plate (5033), and the fourth light guide plate (5034) form a frame structure; and / or, The inner walls of the first light guide plate (5031), the second light guide plate (5032), the third light guide plate (5033), and the fourth light guide plate (5034) taper inward from bottom to top; and / or, A second through hole (5035) is provided at the connection point of the first light guide plate (5031), the second light guide plate (5032), the third light guide plate (5033), and the fourth light guide plate (5034); and / or, The inner walls of the first light guide plate (5031), the second light guide plate (5032), the third light guide plate (5033), and the fourth light guide plate (5034) have a frosted structure; and / or, The second through hole (5035) is located near the top of the light guide mechanism (503).
11. The chemiluminescence imager according to claim 1, characterized in that, The chemiluminescence imager includes a heat dissipation device (600) for dissipating heat from the acquisition device (300); and / or, The chemiluminescence imager includes a display device (700) which is connected to the acquisition device (300).