Device suitable for extracting adipose-derived stromal cells

Abstract

The utility model discloses a device suitable for fat stromal cell extraction, which comprises a bag body, the bag body internally comprises at least a first accommodating cavity and a second accommodating cavity, the first accommodating cavity is used for filling cleaning fluid, and the second accommodating cavity is used for filling digestive juice; the liquid inlet pipe is connected with one side of the accommodating cavity I; the waste liquid pipe is connected with one side of the accommodating cavity I; the liquid outlet pipe is connected with one side of the first containing cavity. According to the device, the fat matrix can be directly cleaned and digested in the same device suitable for extracting the fat matrix cells, an operator does not need to add cleaning liquid and digestion liquid, operation steps are reduced, and cross contamination can be effectively avoided.

Description

TECHNICAL FIELD

[0001] The utility model relates to biological ware's technical field, specifically related to a device suitable for fat matrix cell extraction. BACKGROUND

[0002] The related art for extracting fat matrix cells generally includes the following steps: placing the obtained fat tissue in a sterile cleaning solution for repeated washing, placing the cleaning solution in a centrifugal tube, removing the supernatant after the fat tissue is precipitated, and then transferring the washed fat tissue to a container containing a digestive solution for digestion. After digestion, the cells are collected by centrifugation, and the supernatant is discarded. At this time, the cells in the precipitate are the preliminary extracted fat matrix cells.

[0003] The above operation steps are complicated, and cross contamination may occur during the transfer process, affecting the purity of the final separated fat matrix cells. UTILITY MODEL CONTENT

[0004] The utility model discloses a device suitable for fat matrix cell extraction.

[0005] The utility model discloses a device suitable for fat matrix cell extraction.

[0006] A device suitable for fat matrix cell extraction includes:

[0007] The bag body includes at least one cavity one and cavity two, the cavity one is used for filling cleaning solution, and the cavity two is used for filling digestive solution.

[0008] The liquid inlet pipe is connected to one side of the cavity one.

[0009] The waste liquid pipe is connected to one side of the cavity one.

[0010] The liquid outlet pipe is connected to one side of the cavity one.

[0011] As a further description of the above technical solution, the liquid inlet pipe, the waste liquid pipe and the liquid outlet pipe are all provided with a luer cap.

[0012] As a further description of the above technical solution, the waste liquid pipe is provided with a screen at the connection with the cavity one.

[0013] As a further description of the above technical solution, the mesh number of the screen is 30-50 mesh.

[0014] As a further description of the above technical solution, the mesh number of the screen is 50 mesh.

[0015] As a further description of the above technical solution, the liquid outlet pipe is provided with a switch valve.

[0016] As a further description of the above technical solution, a sealing strip is arranged between the first cavity and the second cavity.

[0017] As a further description of the above technical solution, the sealing strip is a double sealing strip.

[0018] As a further description of the above technical solution, the volume of the first cavity is greater than the volume of the second cavity.

[0019] As a further description of the above technical solution, the bag body is made of flexible material.

[0020] The beneficial effects of the present application are as follows:

[0021] 1. The present application can directly clean and digest the fat matrix in the device suitable for extracting fat matrix cells, without the need for an operator to add cleaning liquid and digestive liquid, reducing the operation steps and effectively avoiding cross contamination.

[0022] To more clearly illustrate the structural features and effects of the present application, the present application will be described in detail below in conjunction with the drawings and specific embodiments. BRIEF DESCRIPTION OF DRAWINGS

[0023] Figure 1 is a schematic view of the device suitable for extracting fat matrix cells provided by the present application.

[0024] Reference signs: 1, liquid outlet pipe; 2, luer cap one; 3, first cavity; 4, sealing strip; 5, second cavity; 6, bag body; 7, screen; 8, waste liquid pipe; 9, luer cap two; 10, liquid inlet pipe; 11, luer cap three; 12, switch valve. DETAILED DESCRIPTION

[0025] To make the purpose, technical solution and advantages of the embodiments of the present application more clear, the technical solution in the embodiments of the present application will be described clearly and completely below in conjunction with the drawings in the embodiments of the present application.

[0026] As shown in Figure 1 In one embodiment, a device suitable for extracting fat matrix cells includes a bag body 6, which includes at least a first cavity 3 and a second cavity 5. The first cavity 3 is used to fill cleaning liquid, and the second cavity 5 is used to fill digestive liquid. A liquid inlet pipe 10 is connected to one side of the first cavity 3, a waste liquid pipe 8 is connected to the lower side of the first cavity 3, and a liquid outlet pipe 1 is connected to the upper side of the first cavity 3. The pipe head of the liquid outlet pipe 1 is connected with a luer cap one 2, the pipe head of the waste liquid pipe 8 is connected with a luer cap two 9, and the pipe head of the liquid inlet pipe 10 is connected with a luer cap three 11.

[0027] By the above technical solution, the fat matrix can be directly washed and digested in the same device suitable for extracting fat matrix cells, without the need for an operator to add washing liquid and digestion liquid, reducing the operation steps, and effectively avoiding cross contamination.

[0028] Optionally, a screen 7 is arranged at the connection between the waste liquid pipe 8 and the first cavity 3, and the purpose is to leave the fat tissue in the first cavity 3 for subsequent processing in the digestion liquid.

[0029] Optionally, the mesh number of the screen 7 is 30-50 mesh, preferably 50 mesh, so that the fat tissue can be effectively intercepted.

[0030] Optionally, a switch valve 12 is arranged on the liquid outlet pipe 1, and after the switch valve 12 is opened, the digested tissue and the liquid containing cells can flow out through the liquid outlet pipe 1. By arranging the switch valve 12, the pipeline of the liquid outlet pipe 1 can be closed during the washing and digestion of the fat tissue, avoiding cross contamination. The specific structure of the switch valve 12 is mature prior art and will not be described here.

[0031] Optionally, a sealing strip 4 is arranged between the first cavity 3 and the second cavity 5, and the washing liquid and the digestion liquid between the two cavities are isolated by arranging the sealing strip 4 after the washing of the fat tissue is completed. After the washing is completed and the washing liquid is discharged, the sealing strip 4 is manually opened, so that the washed fat tissue can be digested. In some embodiments, the sealing strip 4 can also be a double sealing strip 4. By arranging the sealing strip 4, the washing liquid and the digestion liquid can be effectively separated, realizing the functions of washing and digestion in the same bag 6. The specific structure of the sealing strip 4 is widely used in related technology, and will not be described here.

[0032] Optionally, the volume of the first cavity 3 is greater than the volume of the second cavity 5, and in some other embodiments, the volume of the first cavity 3 can be the same as or less than the volume of the second cavity 5. The specific volume can be set according to actual needs.

[0033] Optionally, the bag 6 is made of flexible material, making the entire bag 6 more convenient to carry and easier to operate.

[0034] Working principle: in use, first, the fat tissue is loaded into the bag. Specifically, open the luer cap three 11, connect the syringe loaded with fat tissue with the liquid inlet pipe 10, push the syringe to make the fat tissue enter the bag body 6, and close the luer cap three 11. Then, the fat tissue is cleaned. Specifically, shake the bag to make the fat tissue fully contact with the cleaning liquid in the cavity one 3, so as to achieve the cleaning purpose. After cleaning, open the luer cap two 9, so that the cleaning liquid in the bag and some liquid in the fat tissue flow out along the waste liquid pipe 8; and the fat tissue is retained in the bag due to the existence of the screen 7. After the waste liquid flows out, close the luer cap two 9. Secondly, the fat tissue is digested. Specifically, open the sealing strip 4, so that the digestive liquid in the cavity two 5 mixes with the fat tissue on the left side, and according to the need, the extraction bag can be placed in a 37℃ incubator for digestion. Finally, after the digestion is completed, open the switch valve 12 and the luer cap one 2, so that the digested tissue and the liquid containing cells are transferred out to the centrifugal tube through the liquid outlet pipe 1. After centrifugation of the centrifugal tube, the upper liquid and the fat tissue are removed, and the fat stromal cells are obtained.

[0035] The standard parts used in the utility model can be purchased from the market, the special-shaped parts can be ordered according to the description and the drawings, and the specific connection mode of each part adopts the conventional means such as bolts, rivets and welding in the prior art, the machinery, parts and equipment adopt conventional models in the prior art, and the circuit connection adopts the conventional connection mode in the prior art, which will not be described in detail here, and the contents not described in detail in the specification belong to the prior art known to the person skilled in the art.

[0036] The above description of the disclosed embodiments enables a person skilled in the art to implement or use the utility model. Various modifications to these embodiments will be apparent to those skilled in the art, and the general principles defined herein can be implemented in other embodiments without departing from the spirit or scope of the utility model. Therefore, the utility model will not be limited to these embodiments shown herein, but will conform to the widest scope consistent with the principles and novel features disclosed herein.

Claims

1. A device suitable for adipose matrix cell extraction, characterized in that, The utility model relates to a bag for filling and washing liquid, comprising: a bag body, which comprises at least a first cavity for filling washing liquid and a second cavity for filling digestive liquid; a liquid inlet pipe connected to one side of the first cavity; a waste liquid pipe connected to one side of the first cavity; a liquid outlet pipe connected to one side of the first cavity.

2. The device suitable for adipose stromal cell extraction according to claim 1, characterized in that, The liquid inlet pipe, the waste liquid pipe and the liquid outlet pipe are all provided with a luer cap.

3. The device suitable for adipose stromal cell extraction according to claim 1, wherein, A screen is arranged at the connection between the waste liquid pipe and the first cavity.

4. The device suitable for adipose stromal cell extraction according to claim 3, characterized in that, The screen has a mesh number of 30-50.

5. The device suitable for adipose stromal cell extraction according to claim 4, characterized in that, The screen has a mesh number of 50.

6. The device suitable for adipose stromal cell extraction according to claim 1, wherein, A switch valve is arranged on the liquid outlet pipe.

7. The device suitable for adipose stromal cell extraction according to claim 1, wherein, A sealing strip is arranged between the first cavity and the second cavity.

8. The device suitable for adipose stromal cell extraction according to claim 7, characterized in that, The sealing strip is a double sealing strip.

9. The device suitable for adipose stromal cell extraction according to claim 1, wherein, The volume of the first cavity is larger than that of the second cavity.

10. The device suitable for adipose stromal cell extraction according to claim 1, characterized in that, The bag body is made of flexible material.

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