Separation device for culturing pluripotent stem cells

Abstract

The utility model relates to the technical field of separation for cell culture, and discloses a separation device for culturing pluripotent stem cells, which comprises a base, a separation mechanism is arranged at the top of the base, a fixing mechanism is arranged at the top of the separation mechanism, the separation mechanism comprises a protection box, the protection box is fixedly connected to the top of the base, and the fixing mechanism is arranged at the top of the protection box. A supporting frame is fixedly connected to the front side of the protection box, a protection door is rotationally connected to the front side of the protection box, an ultraviolet disinfection lamp is fixedly connected to the inner side of the protection box, and a first servo motor is fixedly connected to the rear side of the protection box. When the pluripotent stem cells are cultured in the culture tubes and need to be separated, a separation box can be driven to move under the action of a first threaded rod, a moving block and a supporting table, so that the separation box is located on the front side of a protection box, a worker can conveniently place the culture tubes in a limiting frame, and the bottoms of the culture tubes make contact with the top of a placement table.

Description

TECHNICAL FIELD

[0001] The utility model relates to the separation technical field for cell culture, concretely is a kind of separation device for pluripotent stem cell culture. BACKGROUND

[0002] Pluripotent stem cells refer to the stem cells that can differentiate into multiple cell types, but cannot form a complete organism (such as cannot form placental tissue and umbilical cord). They mainly include embryonic stem cells and induced pluripotent stem cells. The separation device for pluripotent stem cell culture is mainly used to separate pluripotent stem cells from organisms for subsequent culture and research. The existing separation device can only separate a single culture tube during use. When the number is large, it needs to be separated multiple times by frequent operation, which affects the efficiency. After separation, the staff takes out the culture tube. Because the culture tube is in a plug-in state, the staff is easy to mix a part again due to large shaking amplitude when taking, which affects the separation effect.

[0003] According to the suspension separation device for macrophage culture (authorization announcement number: CN218321381U) described in the patent network, the suspension separation device for macrophage culture includes "driving assembly, separation assembly, box" and the like to realize separation work.

[0004] In view of the above description, the applicant believes that the following problems exist:

[0005] The suspension separation device for macrophage culture utilizes driving assembly, separation assembly, box and the like to realize separation work. During use of this device, the inner cylinder and the outer cylinder are placed in the slot and placed in the box. The operation space is limited, which is not convenient for operation. When taking after separation, because the exposed parts of the inner cylinder and the outer cylinder are less, it is also easy to mix a part again due to large shaking amplitude when taking, which affects the separation effect. Therefore, the device needs to be improved. UTILITY MODEL CONTENTS

[0006] The utility model aims to provide a separation device for pluripotent stem cell culture to solve the problems raised in the above background technology.

[0007] To achieve the above purpose, the utility model provides the following technical scheme: a separation device for pluripotent stem cell culture, comprising a base, a separation mechanism is arranged on the top of the base, and a fixing mechanism is arranged on the top of the separation mechanism.

[0008] The separation mechanism includes a protective box fixedly connected to the top of the base, a support frame fixedly connected to the front side of the protective box, a protective door rotatably connected to the front side of the protective box, an ultraviolet disinfection lamp fixedly connected to the inner side of the protective box, a first servo motor fixedly connected to the rear side of the protective box, a first threaded rod fixedly connected to the front side of the first servo motor, a moving block threadedly connected to the periphery of the first threaded rod, a support table fixedly connected to the top of the moving block, the support table being slidably connected to the inside of the protective box, a second servo motor fixedly connected to the inside of the support table, a rotating rod fixedly connected to the top of the second servo motor, a separation box fixedly connected to the top of the rotating rod, a support ring fixedly connected to the bottom of the separation box, the support ring being slidably connected to the top of the support table, a limiting frame fixedly connected to the inside of the separation box, a second threaded rod rotatably connected to the inside of the separation box, a knob fixedly connected to the top of the second threaded rod, and a placement table threadedly connected to the periphery of the second threaded rod, the placement table being slidably connected to the inside of the separation box.

[0009] Preferably, the bottom of the support table is slidably connected to the inside of the support frame, the protective box and the support frame are respectively provided with a sliding groove, and the two sliding grooves correspond to each other, the bottom of the support table is slidably connected to the sliding groove, so that the support table can drive the separation box to move under the action of the sliding groove.

[0010] Preferably, the top of the placement table is provided with a sponge pad, and the sliding rod is fixedly connected to the inside of the separation box, so as to support the bottom of the culture tube under the action of the placement table and protect the culture tube through the sponge pad.

[0011] Preferably, a circular groove is formed in the top of the support table, and the support ring is slidably connected to the circular groove, so that the support ring can slide more stably under the action of the circular groove.

[0012] Preferably, a movable groove is formed in the inside of the protective box, and the moving block is slidably connected to the movable groove, so that the moving block can move more stably under the action of the movable groove.

[0013] Preferably, the fixing mechanism includes an electric telescopic rod fixedly connected to the top of the protective box, a mounting seat fixedly connected to the bottom of the electric telescopic rod, a connecting block fixedly connected to the bottom of the mounting seat, a fixing sleeve rotatably connected to the periphery of the connecting block, and a fixing plate fixedly connected to the bottom of the fixing sleeve.

[0014] Preferably, the fixing plate is circular, and a sponge pad is arranged on the top of the fixing plate, so as to further fix the top of the culture tube under the action of the fixing plate.

[0015] Compared with the prior art, the utility model provides a separation device for pluripotent stem cell culture has the following beneficial effects:

[0016] 1, this pluripotent stem cell culture separation device, through the separation mechanism that sets up, when pluripotent stem cell in the culture tube culture needs to carry out separation work after completing, under the action of first threaded rod, moving block and support platform can drive separation box to move, make separation box be in protective box front side, convenient for staff to place culture tube in limiting support, and make culture tube bottom and the top of the placement table contact each other, simultaneously under the action of second threaded rod and slide rod can according to the length of culture tube, the height position of placement table is adjusted, the culture tube bottom is supported and can make the middle position of culture tube be in the middle position of limiting support, guarantee the stability of culture tube, when placing is completed, can make separation box be in protective box under the action of first threaded rod, close the protective door, through ultraviolet disinfection lamp disinfects and sterilizes in protective box, simultaneously under the action of rotary rod and support ring can drive separation box and the culture tube that places to rotate, can carry out separation work, when separation needs to take out culture tube after completing, can drive placement table to move through second threaded rod, drive culture tube to ascend slowly through placement table, improve the efficiency of discharging and can ensure that pluripotent stem cell still maintains integrity after separation, it is convenient for subsequent experimental operation and analysis.

[0017] 2, this pluripotent stem cell culture separation device, through the setting of fixed mechanism, when pluripotent stem cell in the culture tube culture needs to carry out separation work after completing, and culture tube is fixed when completing under the action of placement table and limiting support, can according to the length of culture tube, make electric telescopic rod drive mounting seat and connecting block to move, can make fixed sleeve drive fixed plate to move, make fixed plate top plate in fixed plate and the top of culture tube contact each other, fix culture tube, by pressing the top of culture tube, can reduce the vibration and shaking in culture tube, thereby reduce the possibility of bubble generation, ensure the stability of test tube and reduce interference factors (such as bubble and vibration), can indirectly improve the efficiency and accuracy of cell separation, and fixed sleeve is rotatably connected to the periphery of connecting block, when culture tube rotates, fixed plate can follow rotation, do not affect the fixing effect. BRIEF DESCRIPTION OF DRAWINGS

[0018] In order to more clearly illustrate the technical scheme in the embodiment of the utility model, the following will be to the drawing needed to be used in the embodiment description simple introduction, obviously, the following description in the drawing is only some embodiments of the utility model, for those skilled in the art, under the premise of not paying the creative labor, according to these drawings, other drawings can also be obtained:

[0019] Figure 1 It is the three-dimensional structure schematic diagram of the utility model;

[0020] Figure 2 It is a sectional view structure schematic diagram of the utility model;

[0021] Figure 3 It is a separation mechanism structure schematic diagram;

[0022] Figure 4 It is a protective box sectional view structure schematic diagram;

[0023] Figure 5 It is a support table top structure schematic diagram;

[0024] Figure 6 It is a separation box sectional view structure schematic diagram;

[0025] Figure 7 It is a fixed mechanism structure schematic diagram.

[0026] In the drawing: 1, base; 2, separation mechanism; 3, fixed mechanism; 21, protective box; 22, support frame; 23, protective door; 24, first servo motor; 25, ultraviolet disinfection lamp; 26, support table; 27, moving block; 28, first threaded rod; 29, second servo motor; 291, rotating rod; 292, separation box; 293, support ring; 294, placing table; 295, second threaded rod; 296, knob; 297, sliding rod; 298, limiting frame; 31, electric telescopic rod; 32, mounting seat; 33, connecting block; 34, fixed sleeve; 35, fixed plate. DETAILED DESCRIPTION

[0027] The technical solutions in the embodiments of the utility model will be clearly and completely described below with reference to the drawings in the embodiments of the utility model. Obviously, the described embodiments are only part of the embodiments of the utility model, rather than all the embodiments. Based on the embodiments in the utility model, all other embodiments obtained by those skilled in the art without creative labor fall within the scope of protection of the utility model.

[0028] In the utility model, unless explicitly defined and limited, the terms "mounting", "connection", "connecting", "fixing" and the like should be understood broadly, for example, can be fixed connection, can be detachable connection, or integrated; can be mechanical connection, or electrical connection; can be directly connected, or indirectly connected through an intermediate medium; can be the communication inside two elements or the interaction relationship between two elements. For those skilled in the art, the specific meaning of the above terms in the utility model can be understood according to the specific circumstances.

[0029] The utility model provides the following technical scheme:

[0030] Embodiment one

[0031] Please refer to Figures 1-7 The utility model provides a technical scheme: a separation device for pluripotent stem cell culture, including base 1, base 1 top is provided with separation mechanism 2, separation mechanism 2 top device has fixed mechanism 3,

[0032] Separation mechanism 2 includes protection box 21, protection box 21 fixed connection is in base 1 top, protection box 21 front side is fixedly connected with support frame 22, protection box 21 front side is rotatably connected with protection door 23, protection box 21 inner side is fixedly connected with ultraviolet disinfection lamp 25, protection box 21 rear side is fixedly connected with first servo motor 24, first servo motor 24 front side is fixedly connected with first screw rod 28, first screw rod 28 front side is rotatably connected in protection box 21, first screw rod 28 periphery is screw connected with moving block 27, moving block 27 top is fixedly connected with support table 26, support table 26 bottom is slidably connected in protection box 21 inside, support table 26 inside is fixedly connected with second servo motor 29, second servo motor 29 top is fixedly connected with rotating rod 291, rotating rod 291 top is fixedly connected with separation box 292, separation box 292 bottom is fixedly connected with support ring 293, support ring 293 is slidably connected in support table 26 top, separation box 292 is fixedly connected with limit frame 298 in, separation box 292 inside rotatably connected with second screw rod 295, second screw rod 295 top is fixedly connected with knob 296, second screw rod 295 periphery is screw connected with placing table 294, and placing table 294 inside is slidably connected with sliding rod 297.

[0033] Support table 26 bottom is slidably connected in support frame 22 inside, and the protection box 21 and support frame 22 are respectively provided with the sliding slot, and the two sliding slots correspond to each other, and the support table 26 bottom is slidably connected in the sliding slot, and the support table 26 can be moved under the action of the sliding slot, and the placing table 294 top is provided with sponge pad, and the sliding rod 297 is fixedly connected in the separation box 292, and the placing table 294 can be supported under the action of the placing table 294, and the culture tube can be protected by the sponge pad, and the support table 26 top is provided with circular groove, and the support ring 293 is slidably connected in the circular groove, so that the support ring 293 is more stable under the action of the circular groove, and the protection box 21 is provided with movable slot, and the moving block 27 is slidably connected in the movable slot, so that the moving block 27 is more stable under the action of the movable slot.

[0034] Embodiment two

[0035] Please refer to Figures 1-7And on the basis of embodiment one, further get fixed mechanism 3 including electric telescopic rod 31, electric telescopic rod 31 fixedly connected to the top of the protective box 21, electric telescopic rod 31 bottom fixedly connected with mounting seat 32, mounting seat 32 bottom fixedly connected with connecting block 33, connecting block 33 periphery rotationally connected with fixed sleeve 34, fixed sleeve 34 bottom fixedly connected with fixed plate 35, fixed plate 35 is circular, and the inner top of fixed plate 35 is provided with sponge pad, which is convenient for further fixing the top of the culture tube under the action of fixed plate 35.

[0036] In actual operation, when the device is used, when the pluripotent stem cells are cultured in the culture tube and need to be separated, the first servo motor 24 drives the moving block 27 connected with the first threaded rod 28 to move, so that the moving block 27 drives the support table 26 to move, and the bottom of the support table 26 slides inside the protective box 21 and the support frame 22, that is, the separation box 292 is driven to move by the support table 26, so that the separation box 292 is located at the front side of the protective box 21, the staff can place the culture tube in the limiting frame 298, and the bottom of the culture tube is in contact with the top of the placement table 294, and according to the length of the culture tube, the knob 296 is rotated, the second threaded rod 295 drives the placement table 294 to move, and the placement table 294 slides on the slide rod 297, so that the height of the placement table 294 is adjusted, the middle position of the culture tube is located at the middle position of the limiting frame 298, and the stability of the culture tube is ensured. When the placement is completed, the separation box 292 is located in the protective box 21 under the action of the first threaded rod 28, the protective door 23 is closed, the ultraviolet disinfection lamp 25 is powered by the external power supply equipment, and the ultraviolet disinfection lamp 25 disinfects and sterilizes the inside of the protective box 21;

[0037] At the same time, the mounting seat 32 and the connecting block 33 are driven by the electric telescopic rod 31 to move, so that the fixed sleeve 34 drives the fixed plate 35 to move, and the inner top of the fixed plate 35 is in contact with the top of the culture tube, so that the culture tube is fixed. By pressing the top of the culture tube, the vibration and shaking in the culture tube can be reduced, thereby reducing the possibility of bubble generation, ensuring the stability of the test tube and reducing the interference factors (such as bubbles and vibration), which can indirectly improve the efficiency and accuracy of cell separation;

[0038] When the fixing is completed, the separation tank 292 fixedly connected to the top of the rotating rod 291 driven by the second servo motor 29 can be rotated, and the support ring 293 is driven by the separation tank 292 to slide on the top of the support table 26, so that the placed culture tube is rotated to separate the pluripotent stem cells. The fixed sleeve 34 is rotatably connected to the periphery of the connecting block 33, and the fixed plate 35 can rotate when the culture tube rotates, without affecting the fixing effect. When the separation is completed, the separation tank 292 can be repositioned in front of the protection tank 21. Under the action of the second threaded rod 295 and the sliding rod 297, the placement table 294 is moved to automatically eject the placed culture tube, which is convenient for the workers to take and can ensure that the pluripotent stem cells remain intact after separation, which is convenient for subsequent experimental operation and analysis.

[0039] It should be noted that, in this document, the terms such as first and second are used merely to distinguish one entity or operation from another, and do not necessarily require or imply that these entities or operations exist in any actual relationship or order. Moreover, the terms "include", "contain" or any other variants thereof are intended to cover non-exclusive inclusion, so that the process, method, article or equipment including a series of elements not only includes those elements, but also includes other elements not explicitly listed or inherent to such process, method, article or equipment. Without more limitations, the element defined by the statement "including a" does not exclude the presence of other identical elements in the process, method, article or equipment including the element.

Claims

1. A separation device for pluripotent stem cell culture comprising a base (1), characterized in that: The top of the base (1) is provided with a separation mechanism (2), and the top of the separation mechanism (2) is provided with a fixing mechanism (3); The separation mechanism (2) comprises a protective box (21), the protective box (21) is fixedly connected to the top of the base (1), the front side of the protective box (21) is fixedly connected with a support frame (22), the front side of the protective box (21) is rotatably connected with a protective door (23), the inner side of the protective box (21) is fixedly connected with an ultraviolet disinfection lamp (25), the rear side of the protective box (21) is fixedly connected with a first servo motor (24), the front side of the first servo motor (24) is fixedly connected with a first threaded rod (28), the front side of the first threaded rod (28) is rotatably connected in the protective box (21), the periphery of the first threaded rod (28) is threadedly connected with a moving block (27), the top of the moving block (27) is fixedly connected with a support table (26), the bottom of the support table (26) is slidably connected in the protective box (21), the inside of the support table (26) is fixedly connected with a second servo motor (29), and the top of the second servo motor (29) is fixedly connected with a rotating rod (291). The top of the rotating rod (291) is fixedly connected with a separation box (292), the bottom of the separation box (292) is fixedly connected with a support ring (293), the support ring (293) is slidably connected to the top of the support table (26), the separation box (292) is fixedly connected with a limiting frame (298) in the inside, the inside of the separation box (292) is rotatably connected with a second threaded rod (295), the top of the second threaded rod (295) is fixedly connected with a knob (296), the periphery of the second threaded rod (295) is threadedly connected with a placing table (294), and the inside of the placing table (294) is slidably connected with a sliding rod (297).

2. The separation device for pluripotent stem cell culture according to claim 1, wherein: The bottom of the support table (26) is slidably connected in the inside of the support frame (22), the protective box (21) and the support frame (22) are respectively provided with a sliding groove in the inside, and the two sliding grooves correspond to each other, and the bottom of the support table (26) is slidably connected in the sliding groove.

3. The device for isolating pluripotent stem cells according to claim 1, wherein: The top of the placing table (294) is provided with a sponge pad, and the sliding rod (297) is fixedly connected in the separation box (292).

4. The separation device for pluripotent stem cell culture according to claim 1, wherein: The top of the support table (26) is provided with a circular groove, and the support ring (293) is slidably connected in the circular groove.

5. The isolated device for pluripotent stem cell culture according to claim 1, wherein: The inside of the protective box (21) is provided with a movable groove, and the movable block (27) is slidably connected in the movable groove.

6. The isolated device for pluripotent stem cell culture according to claim 1, wherein: The fixing mechanism (3) comprises an electric telescopic rod (31), the electric telescopic rod (31) is fixedly connected to the top of the protective box (21), the bottom of the electric telescopic rod (31) is fixedly connected with a mounting seat (32), the bottom of the mounting seat (32) is fixedly connected with a connecting block (33), the periphery of the connecting block (33) is rotatably connected with a fixing sleeve (34), and the bottom of the fixing sleeve (34) is fixedly connected with a fixed plate (35).

7. The device for isolating pluripotent stem cells according to claim 6, wherein: The fixed plate (35) is circular, and the inside of the fixed plate (35) is provided with a sponge pad.

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