Molecular detection all-in-one machine

Abstract

The utility model belongs to the field of molecular diagnosis, and particularly relates to a molecular detection all-in-one machine which comprises a rack and a sample treatment device, a PCR detection device and a sample lifting device which are arranged on the rack, sample tube cup separation, nucleic acid extraction, PCR system construction and PCR amplification detection are integrated, and the nucleic acid detection process can be achieved without multiple devices. The sample treatment space and the detection space located below the sample treatment space are arranged on the rack, the sample treatment device and the PCR detection device are arranged in the sample treatment space and the detection space respectively, samples are transferred between the sample treatment device and the PCR detection device through the sample lifting device, the space is reasonably arranged, and the detection efficiency is improved. The whole molecular detection all-in-one machine is more compact in structure and smaller in occupied area.

Description

TECHNICAL FIELD

[0001] The present application relates to the technical field of molecular diagnosis, and particularly relates to a molecular detection all-in-one machine. BACKGROUND

[0002] Molecular diagnosis technology is a technical means capable of quickly and accurately diagnosing a pathogen, including multiple links such as pathogen sample pretreatment, nucleic acid extraction, PCR amplification, fluorescent labeling and detection, and result analysis. In the field of HPV screening, a traditional detection method usually needs to cooperate with multiple devices to respectively perform sample tube cupping, pipetting, nucleic acid extraction, PCR system construction, PCR amplification detection and other steps. In the prior art, most molecular diagnosis instruments are semi-automatic instruments, and in the process of molecular diagnosis, manual participation is needed for sample transfer and other operations. The sample is transferred between devices, the operation is complex, the efficiency is low, and human errors are prone to occur.

[0003] In the related art, an automatic molecular diagnosis all-in-one machine integrates sample pretreatment, nucleic acid extraction and PCR detection instruments and other related devices together, and performs cupping, nucleic acid extraction, sample transfer and other operations by a mechanical arm instead of manual operation. Since the sample pretreatment, nucleic acid extraction and PCR detection instruments and other related devices are arranged on the rack table, there is a problem of unreasonable space structure layout and non-compact structure, which causes the entire machine to occupy a large area. CONTENT OF THE UTILITY MODEL

[0004] Embodiments of the present application provide a molecular detection all-in-one machine, which is used to solve the problem of unreasonable space structure layout and non-compact structure of the existing molecular diagnosis device, which causes the entire machine to occupy a large area.

[0005] To achieve the above-mentioned purpose, the present application provides a molecular detection all-in-one machine, which comprises a rack, a sample processing device, a PCR detection device and a sample lifting device. The rack is provided with a sample processing space and a detection space located below the sample processing space. The sample processing device is arranged in the sample processing space and is used for cupping, extracting nucleic acid and constructing a PCR reaction system for a sample. The PCR detection device is arranged in the detection space and is used for PCR amplification detection. The sample lifting device is arranged between the sample processing device and the PCR detection device and is used for transferring a sample between the sample processing device and the PCR detection device.

[0006] Optionally, the rack comprises a frame body and a bearing plate arranged on the frame body. A region of the frame body located above the bearing plate forms the sample processing space, a region of the frame body located below the bearing plate forms the detection space, and the bearing plate is provided with a relief hole for the sample lifting device to transfer a sample up and down.

[0007] Optionally, the bearing plate is further provided with a waste discarding port, and a waste collecting barrel is arranged in the detection space below the waste discarding port.

[0008] Optionally, the sample processing device comprises a cup dividing module, a nucleic acid extraction module, a system construction module and a mechanical arm module, the cup dividing module is used for cup dividing of samples, the nucleic acid extraction module is used for nucleic acid extraction of the cup-divided samples, and the system construction module is used for construction of a PCR reaction system; the mechanical arm module has a gripper and a pipette gun capable of moving in the sample processing space.

[0009] Optionally, the sample processing device further comprises a consumable storage module, the consumable storage module is arranged in the sample processing space, and the consumable storage module is provided with a partitioned placement seat for storing a deep well plate, a PCR plate, reagents and a gun head; the mechanical arm module is further capable of transferring consumables between the consumable storage module and the cup dividing module, between the consumable storage module and the nucleic acid extraction module, and between the consumable storage module and the system construction module.

[0010] Optionally, the cup dividing module comprises a tube rack, a clamping module and a deep well plate rack, the tube rack is used for bearing sample tubes, the clamping module is used for clamping sample tubes, the gripper is used for transferring sample tubes to the clamping module and opening the tube cover of the sample tubes in cooperation with the clamping module, and the pipette gun is used for transferring samples in the sample tubes to the deep well plate on the deep well plate rack for cup dividing operation.

[0011] Optionally, the mechanical arm module comprises a first linear module, a second linear module arranged on the first linear module and a plurality of lifting modules arranged on the second linear module, the first linear module is arranged in the sample processing space and located above the cup dividing module and the nucleic acid extraction module, each lifting module is capable of moving in a first direction under the drive of the second linear module and moving in a second direction under the drive of the first linear module, the first direction intersects the second direction; the gripper and the pipette gun are arranged on different lifting modules respectively, the gripper is capable of moving up and down under the drive of the lifting module connected thereto, and the gripper is further capable of rotating relative to the lifting module; the pipette gun is capable of moving up and down under the drive of the lifting module connected thereto.

[0012] Optionally, the sample lifting device comprises a lifting mechanism and a lifting tray arranged on the lifting mechanism, the lifting tray is capable of transferring a PCR plate between the sample processing space and the detection space under the action of the lifting mechanism.

[0013] Optionally, the PCR detection device comprises a film sealing instrument, a PCR detection instrument and a transfer module; the film sealing instrument is used for sealing the film of the PCR plate before PCR detection; the transfer module is used for transferring the PCR plate between the sample lifting device, the film sealing instrument and the PCR detection instrument.

[0014] Optionally, the film sealing instrument and the PCR detection instrument are arranged side by side in the detection space, the sample lifting device is arranged between the film sealing instrument and the PCR detection instrument, the transfer module is located on the same side of the film sealing instrument, the sample lifting device and the PCR detection instrument, the transfer module comprises a linear module, a lifting unit arranged on the linear module and a clamping jaw arranged on the lifting unit, the clamping jaw moves up and down under the action of the lifting unit and moves between the film sealing instrument, the sample lifting device and the PCR detection instrument under the action of the linear module.

[0015] The molecular detection all-in-one machine provided by the present application has the advantages that: compared with the prior art, the molecular detection all-in-one machine of the present application comprises a rack and a sample processing device, a PCR detection device and a sample lifting device arranged on the rack, which integrates sample tube cupping, nucleic acid extraction, PCR system construction and PCR amplification detection, and nucleic acid detection process can be realized without multiple devices. By arranging the sample processing device and the PCR detection device in the sample processing space and the detection space respectively and transferring the sample between the sample processing device and the PCR detection device through the sample lifting device, the space is reasonably arranged, the structure of the whole molecular detection all-in-one machine is more compact, and the occupied area is smaller. BRIEF DESCRIPTION OF DRAWINGS

[0016] In order to more clearly illustrate the technical solutions in the embodiments of the present application or the prior art, the drawings needed to be used in the embodiments or the prior art description will be briefly introduced. Obviously, the drawings in the following description are only some embodiments of the present application, and other drawings can be obtained by those skilled in the art without creative labor.

[0017] Among them:

[0018] Figure 1 is the overall structure schematic diagram of the molecular detection all-in-one machine shown in an embodiment of the present application;

[0019] Figure 2 is the front view of the molecular detection all-in-one machine shown in an embodiment of the present application;

[0020] Figure 3is a structural schematic diagram of a sample processing device in a molecular detection all-in-one machine according to an embodiment of the present application;

[0021] Figure 4 is a top view of a cup dividing module, a nucleic acid extraction module, and a system construction module on a bearing plate in a sample processing device of a molecular detection all-in-one machine according to an embodiment of the present application;

[0022] Figure 5 is a structural schematic diagram of a PCR detection device and a sample lifting device in a detection space in a molecular detection all-in-one machine according to an embodiment of the present application.

[0023] Main element symbol explanation:

[0024] 100, rack; 110, frame body; 120, bearing plate; 1201, avoiding hole; 1202, waste discarding port;

[0025] 200, sample processing device; 210, cup dividing module; 211, tube rack; 212, clamping module; 213, deep-well plate rack; 220, nucleic acid extraction module; 230, system construction module; 240, mechanical arm module; 241, gripper; 242, pipette; 250, consumable storage module;

[0026] 300, PCR detection device; 310, film sealing instrument; 320, PCR detector; 330, transfer module; 331, linear module; 332, lifting unit; 333, clamping jaw;

[0027] 400, sample lifting device; 410, lifting mechanism; 420, lifting tray;

[0028] 500, waste collection barrel. DETAILED DESCRIPTION

[0029] In order to facilitate the understanding of the present application, the present application will be described more fully below with reference to the accompanying drawings. The preferred embodiments of the present application are shown in the drawings. However, the present application can be implemented in many other different forms and is not limited to the embodiments described herein. On the contrary, the purpose of providing these embodiments is to make the disclosure of the present application more thorough and comprehensive.

[0030] It should be noted that when an element is referred to as being "fixed to" or "disposed on" another element, it can be directly on the other element or indirectly on the other element. When an element is referred to as being "connected to" another element, it can be directly connected to the other element or indirectly connected to the other element.

[0031] It should be understood that the terms "length", "width", "upper", "lower", "front", "rear", "left", "right", "vertical", "horizontal", "top", "bottom", "inner", "outer", etc., indicate the orientation or positional relationship based on the orientation or positional relationship shown in the accompanying drawings. They are only for the convenience of describing this application and simplifying the description, and do not indicate or imply that the device or element referred to must have a specific orientation, or be constructed and operated in a specific orientation. Therefore, they should not be construed as limitations on this application.

[0032] Furthermore, the terms "first" and "second" are used for descriptive purposes only and should not be construed as indicating or implying relative importance or implicitly specifying the number of technical features indicated. Thus, a feature defined as "first" or "second" may explicitly or implicitly include one or more of that feature. In the description of this application, "multiple" means two or more, unless otherwise explicitly specified.

[0033] Unless otherwise defined, all technical and scientific terms used herein have the same meaning as commonly understood by one of ordinary skill in the art to which this application belongs. The terminology used herein is for the purpose of describing particular embodiments only and is not intended to be limiting of the application.

[0034] Embodiments of this application provide a molecular diagnostic integrated machine that can be used for HPV screening, respiratory and maternal and child testing, etc., providing strong support for clinical diagnosis and treatment. Figures 1-2 As shown, the molecular diagnostic instrument includes a frame 100, a sample processing device 200, a PCR detection device 300, and a sample lifting device 400. The frame 100 has a sample processing space and a detection space located below the sample processing space. The sample processing device 200 is located in the sample processing space and is used to divide the sample into cups, extract nucleic acids, and construct a PCR reaction system. The PCR detection device 300 is located in the detection space and is used for PCR amplification and detection. The sample lifting device 400 is located between the sample processing space and the detection space and is used to transfer samples between the sample processing device 200 and the PCR detection device 300.

[0035] In this embodiment, the integrated molecular detection machine combines sample tube dispensing, nucleic acid extraction, PCR system construction, and PCR amplification detection into one unit, enabling nucleic acid detection without the need for multiple devices. By setting up a sample processing space and a detection space below the sample processing space on the rack 100, the sample processing device 200 and the PCR detection device 300 are respectively arranged in the sample processing space and the detection space. The sample is transferred between the sample processing device 200 and the PCR detection device 300 through the sample lifting device 400. The rational spatial layout makes the entire integrated molecular detection machine more compact and occupies less space.

[0036] In an embodiment, as shown in Figures 1-3 The rack 100 includes a frame body 110 and a bearing plate 120 arranged on the frame body 110. The area above the bearing plate 120 is a sample processing space, and the area below the bearing plate 120 is a detection space. The bearing plate 120 is provided with an avoidance hole 1201 for the sample lifting device 400 to transfer the sample up and down.

[0037] Further, the rack 100 further includes a closed machine body shell (not shown in the figure), and the frame body 110 is arranged in the machine body shell. The machine body shell mainly realizes isolation from the outside world to prevent the overflow of pollutants. The machine body shell can be provided with a hatch, an observation window, a touch screen, and other external interaction interfaces.

[0038] In addition, the bearing plate 120 is also provided with a waste disposal port 1202, and a waste collection barrel 500 is arranged in the detection space below the waste disposal port 1202.

[0039] In an embodiment, as shown in Figures 1-4 The sample processing device 200 includes a cup dividing module 210, a nucleic acid extraction module 220, a system construction module 230, and a mechanical arm module 240. The cup dividing module 210, the nucleic acid extraction module 220, and the system construction module 230 are arranged on the bearing plate 120. The mechanical arm module 240 is arranged on the frame body 110 above the bearing plate 120. The cup dividing module 210 is used for cup dividing of the sample. The nucleic acid extraction module 220 is used for nucleic acid extraction of the cup-divided sample. The system construction module 230 is used for construction of a PCR reaction system. The mechanical arm module 240 has a gripper 241 and a pipette gun 242 that can move in the sample processing space.

[0040] The sample processing device 200 further includes a consumable storage module 250. The consumable storage module 250 is arranged in the sample processing space. The consumable storage module 250 is provided with a partitioned placement seat for storing a deep well plate, a PCR plate, reagents (such as extraction reagents and PCR amplification reagents), and a tip (also known as a Tip head). The mechanical arm module 240 can also transfer consumables between the consumable storage module 250 and the cup dividing module 210, between the consumable storage module 250 and the nucleic acid extraction module 220, and between the consumable storage module 250 and the system construction module 230.

[0041] The dispensing module 210 includes a tube rack 211, a clamping module 212, and a deep-well plate rack 213. The tube rack 211 is used to support the sample tubes, and the clamping module 212 is used to clamp the sample tubes. The clamping module 212 can be a clamping mechanism that can be opened and closed, including but not limited to electric grippers and pneumatic grippers, as long as it can clamp and fix the tube body of the sample tube. The gripper 241 is used to transfer the sample tube to the clamping module 212 and cooperate with the clamping module 212 to open the tube cap of the sample tube. The pipette 242 is used to transfer the sample in the sample tube to the deep-well plate on the deep-well plate rack 213 for dispensing.

[0042] During operation, gripper 241 picks up the deep-well plate used for dispensing at consumable storage module 250 and transfers it to deep-well plate holder 213. Gripper 241 picks up the sample tube from the tube clamp and transfers it to clamping module 212. Clamping module 212 actuates to clamp the sample tube body. Gripper 241 picks up the tube cap and rotates it to open the tube cap. At the same time, pipette 242 moves to consumable storage module 250 to press the pipette tip, then moves to clamping module 212 to draw up sample liquid, and moves to deep-well plate for dispensing. After dispensing, pipette 242 moves to waste disposal port 1202, removes the used pipette tip, and the discarded pipette tip falls into waste collection bin 500 below, completing the dispensing operation. After the sample is dispensed, the gripper 241 picks up the deep-well plate containing the sample solution and moves it to the nucleic acid extraction module 220 for nucleic acid extraction. The nucleic acid extraction module 220 can use either the upper or lower magnetic bead method for nucleic acid extraction, which is not limited here. Preferably, the nucleic acid extraction module 220 also includes a heating and shaking module to heat the liquid in the deep-well plate to meet the temperature requirements for extraction. The nucleic acid extracted by the nucleic acid extraction module 220 is transferred to the system construction module 230 via a pipette 242 and mixed with reagents in a PCR plate to construct the PCR reaction system.

[0043] In one specific embodiment, such as Figures 1-3 As shown, the robotic arm module 240 includes a first linear module, a second linear module disposed on the first linear module, and multiple lifting modules disposed on the second linear module. The first linear module is disposed within the sample processing space and is located above the dispensing cup module 210 and the nucleic acid extraction module 220. Each lifting module can move along a first direction under the drive of the second linear module and move along a second direction under the drive of the first linear module. The first direction and the second direction intersect. The gripper 241 and the pipette 242 are respectively disposed on different lifting modules. The gripper 241 can move up and down under the drive of the lifting module connected to it, and the gripper 241 can also rotate relative to the lifting module. The pipette 242 can move up and down under the drive of the lifting module connected to it.

[0044] Specifically, the gripper 241 can adopt a rotating gripper, and the distance between the two clamping arms of the gripper 241 is adjustable to clamp objects of different widths, thereby realizing clamping of consumables such as sample tubes, deep-well plates and PCR plates. The pipette 242 can adopt a single-head pipette or a multi-head pipette, which is not limited here.

[0045] Preferably, the first direction is perpendicular to the second direction, and the plane where the first direction and the second direction are located is parallel to the plane where the carrier plate 120 is located. For the convenience of understanding and description, an X-Y-Z three-axis coordinate system is established in the figure, wherein the first direction is the direction where the X-axis is located, the second direction is the direction where the Y-axis is located, and the vertical direction is the direction where the Z-axis is located.

[0046] In an embodiment, as shown in Figures 1-2 and Figure 5 , the sample lifting device 400 includes a lifting mechanism 410 and a lifting tray 420 arranged on the lifting mechanism 410, and the lifting tray 420 can be transferred between the sample processing space and the detection space under the action of the lifting mechanism 410.

[0047] As shown in Figure 4 , the lifting tray 420 is driven by the lifting mechanism 410 to move into the avoiding hole 1201 on the carrier plate 120, the gripper 241 clamps the PCR plate constructed by the system construction module 230 and places it on the lifting tray 420, and the lifting mechanism 410 drives the lifting tray 420 to lower the PCR plate to the PCR detection device 300.

[0048] In an embodiment, as shown in Figures 1-2 and Figure 5 , the PCR detection device 300 includes a film sealing instrument 310, a PCR detection instrument 320 and a transfer module 330; the film sealing instrument 310 is used for sealing the film of the PCR plate before PCR detection; and the transfer module 330 is used for transferring the PCR plate between the sample lifting device 400, the film sealing instrument 310 and the PCR detection instrument 320.

[0049] During work, the transfer module 330 clamps the PCR plate on the sample lifting device 400 and transfers it to the film sealing instrument 310, the film sealing instrument 310 seals the opening of the PCR plate, and after the sealing is completed, the transfer module 330 clamps the sealed PCR plate and transfers it to the PCR detection instrument 320 for PCR amplification detection.

[0050] In a specific embodiment, as shown in Figures 1-2 and Figure 5As shown, the film sealing instrument 310 and the PCR detector 320 are arranged side by side in the detection space, the sample lifting device 400 is arranged between the film sealing instrument 310 and the PCR detector 320, the transfer module 330 is located on the same side of the film sealing instrument 310, the sample lifting device 400 and the PCR detector 320, the transfer module 330 includes a linear module 331, a lifting unit 332 arranged on the linear module 331 and a clamping jaw 333 arranged on the lifting unit 332, the clamping jaw 333 moves up and down under the action of the lifting unit 332 and moves between the film sealing instrument 310, the sample lifting device 400 and the PCR detector 320 under the action of the linear module 331, so as to realize the transfer of the PCR plate between the sample lifting device 400, the film sealing instrument 310 and the PCR detector 320.

[0051] By arranging the sample lifting device 400 between the film sealing instrument 310 and the PCR detector 320 and arranging the transfer module 330 on the same side of the film sealing instrument 310, the sample lifting device 400 and the PCR detector 320, on the one hand, the movement stroke of the transfer module 330 can be saved, and on the other hand, the transfer module 330 only needs to clamp the PCR plate to move horizontally and lift up and down, which can be realized by only two linear modules, simplifying the structure and saving the cost.

[0052] The technical features of the above embodiments can be combined in any manner. To make the description concise, all possible combinations of the technical features in the above embodiments are not described, but as long as the combinations of the technical features do not exist contradictory, they should be considered as the scope of the present application.

[0053] The above embodiments only express several implementation manners of the present application, and the description is more specific and detailed, but it should not be understood as a limitation on the scope of the application. It should be pointed out that for ordinary skilled in the art, without departing from the concept of the present application, several modifications and improvements can be made, which are all within the protection scope of the present application. Therefore, the protection scope of the present application should be subject to the appended claims.

Claims

1. A molecular detection all-in-one machine, characterized in that, The device comprises a rack (100), a sample processing device (200), a PCR detection device (300) and a sample lifting device (400); the rack (100) is provided with a sample processing space and a detection space below the sample processing space; the sample processing device (200) is arranged in the sample processing space, and is used for cupping, extracting nucleic acid and constructing a PCR reaction system for a sample; the PCR detection device (300) is arranged in the detection space, and is used for PCR amplification detection; and the sample lifting device (400) is arranged between the sample processing device (200) and the PCR detection device (300), and is used for transferring a sample between the sample processing device (200) and the PCR detection device (300).

2. The molecular testing kiosk of claim 1, wherein, The rack (100) comprises a frame body (110) and a bearing plate (120) arranged on the frame body (110); a region of the frame body (110) above the bearing plate (120) forms the sample processing space, a region of the frame body (110) below the bearing plate (120) forms the detection space, and the bearing plate (120) is provided with an avoiding hole (1201) for the sample lifting device (400) to transfer a sample up and down.

3. The molecular testing kiosk of claim 2, wherein, The bearing plate (120) is further provided with a waste discarding port (1202), and a waste collection barrel (500) is arranged in the detection space below the waste discarding port (1202).

4. The molecular testing kiosk of claim 1, wherein, The sample processing device (200) comprises a cupping module (210), a nucleic acid extraction module (220), a system construction module (230) and a mechanical arm module (240); the cupping module (210) is used for cupping a sample, the nucleic acid extraction module (220) is used for extracting nucleic acid from the cupped sample, and the system construction module (230) is used for constructing a PCR reaction system; and the mechanical arm module (240) has a gripper (241) and a pipette gun (242) capable of moving in the sample processing space.

5. The molecular testing kiosk of claim 4, wherein, The sample processing device (200) further comprises a consumable storage module (250); the consumable storage module (250) is arranged in the sample processing space, and is provided with a partitioned placement seat for storing a deep-well plate, a PCR plate, reagents and a pipette tip; and the mechanical arm module (240) can further transfer consumables between the consumable storage module (250) and the cupping module (210), between the consumable storage module (250) and the nucleic acid extraction module (220), and between the consumable storage module (250) and the system construction module (230).

6. The molecular testing kiosk of claim 4, wherein, The cup dividing module (210) comprises a tube rack (211) for carrying sample tubes, a clamping module (212) for clamping sample tubes, a gripper (241) for transferring sample tubes to the clamping module (212) and opening the tube cover of the sample tubes in cooperation with the clamping module (212), and a pipette (242) for transferring samples in the sample tubes to a deep-well plate on the deep-well plate rack (213) for cup dividing.

7. The molecular testing kiosk of claim 4, wherein, The mechanical arm module (240) comprises a first linear module, a second linear module arranged on the first linear module, and a plurality of lifting modules arranged on the second linear module. The first linear module is arranged in the sample processing space and above the cup dividing module (210) and the nucleic acid extraction module (220). Each lifting module can move in a first direction under the drive of the second linear module and move in a second direction under the drive of the first linear module. The first direction intersects the second direction. The gripper (241) and the pipette (242) are arranged on different lifting modules, respectively. The gripper (241) can move up and down under the drive of the lifting module connected thereto, and the gripper (241) can also rotate relative to the lifting module. The pipette (242) can move up and down under the drive of the lifting module connected thereto.

8. The molecular testing kiosk of claim 1, wherein, The sample lifting device (400) comprises a lifting mechanism (410) and a lifting tray (420) arranged on the lifting mechanism (410). The lifting tray (420) can be transferred between the sample processing space and the detection space under the action of the lifting mechanism (410).

9. The molecular testing lab of any one of claims 1-8, wherein, The PCR detection device (300) comprises a film sealing instrument (310), a PCR detection instrument (320), and a transfer module (330). The film sealing instrument (310) is used for sealing the PCR plate before PCR detection. The transfer module (330) is used for transferring the PCR plate between the sample lifting device (400), the film sealing instrument (310), and the PCR detection instrument (320).

10. The molecular testing kiosk of claim 9, wherein, The sealing film instrument (310) and the PCR detector (320) are arranged side by side in the detection space, the sample lifting device (400) is arranged between the sealing film instrument (310) and the PCR detector (320), the transfer module (330) is located on the same side of the sealing film instrument (310), the sample lifting device (400) and the PCR detector (320), the transfer module (330) comprises a linear module (331), a lifting unit (332) arranged on the linear module (331) and a clamping jaw (333) arranged on the lifting unit (332), the clamping jaw (333) moves up and down under the action of the lifting unit (332) and moves between the sealing film instrument (310), the sample lifting device (400) and the PCR detector (320) under the action of the linear module (331).

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