Tissue culture bag flaring assembly for anoectochilus formosanus tissue culture
By designing an expansion assembly for tissue culture bags and using brackets and bolts to fix the opening of the tissue culture bags, the problem of inconvenient operation during the addition of culture medium solution and inoculation was solved, enabling single-person operation and efficient culture medium solution pouring and inoculation.
Patent Information
- Application Number
- CN202423291422.7
- Authority / Receiving Office
- CN · China
- Patent Type
- Utility models(China)
- Current Assignee / Owner
- Filing Date
- 2024-12-31
- Publication Date
- 2025-12-09
- Estimated Expiration
- 2034-12-31
AI Technical Summary
Adding culture medium to the tissue culture bag requires two people to open the bag opening, and one hand is needed to open the bag opening during inoculation, resulting in high labor costs and low operational efficiency.
Design a tissue culture bag flaring assembly, including a bracket, a horizontal bar, and bolts. The mounting holes on both sides of the opening section of the tissue culture bag are fixed by the bolts, enabling single-person operation. The height and spacing of the horizontal bar can be adjusted to accommodate tissue culture bags of different sizes, ensuring that the opening is stably opened.
It enables single-person operation of adding culture medium solution, improving operational efficiency, reducing manpower consumption, and freeing up the hand to open the bag during inoculation, thus improving overall operational efficiency.
Smart Images
Figure CN223639886U_ABST
Abstract
Description
Technical Field
[0001] This utility model relates to a tissue culture bag flaring component for tissue culture of Anoectochilus roxburghii. Background Technology
[0002] Golden Thread Orchid (Anoectochilus roxburghii) is a genus of orchids belonging to the Orchidaceae family. It possesses medicinal properties, including liver protection, treatment of dark spots, bronchitis, hypertension, and diabetes, and has high economic value, leading to its increasing cultivation. To improve yield, tissue culture is commonly used to rapidly increase the number of seedlings. Tissue culture bottles are commonly used for propagation, but these bottles have a fixed structure, are large in size, occupy a significant area, and are inconvenient to move. Often, the bottles are broken when harvesting seedlings, resulting in bottle loss.
[0003] Therefore, tissue culture bags are now being used to culture Anoectochilus roxburghii. These bags are flexible, have a small overall volume after inoculation, require little space, and are easy to transfer. Seedlings can be retrieved simply by opening the self-sealing opening of the bag, making it quite convenient. However, the following problems exist: When pouring the heated culture medium into the tissue culture bag, two people are often required—one to open the bag and the other to pour the medium, increasing manpower and making the operation inconvenient. Furthermore, when inoculating Anoectochilus roxburghii into the solidified culture medium after high-temperature sterilization, the operator needs to open the bag with one hand and inoculate with the other, especially when inoculating a large number of seedlings in each bag. The hand holding the bag open needs to be frequently changed, resulting in low efficiency and inconvenience. Therefore, these issues need to be addressed. Summary of the Invention
[0004] To address the issues of labor-intensive tasks such as requiring a dedicated person to open the bag opening when adding culture medium, and the inefficiency caused by the need to use one hand to open the bag opening during inoculation and the frequent need to change the opening position, this invention provides a tissue culture bag widening component for Anoectochilus roxburghii tissue culture.
[0005] The tissue culture bag flaring assembly for tissue culture of *Anoectochilus roxburghii* described in this utility model includes a tissue culture bag, a pair of supports, several horizontal rods connected between the pair of supports, and several bolts. The tissue culture bag is a self-sealing bag with a self-sealing part, which divides the bag body into a lower body part and an upper opening section. At least one through first mounting hole is provided on each of the opposite side walls of the opening section. Several through second mounting holes are evenly distributed on the horizontal rods. The diameters of the first and second mounting holes are both larger than the bolt screw diameter and smaller than the bolt head diameter.
[0006] In order to facilitate the adjustment of the height of the horizontal bar according to the height of the tissue culture bag, each support includes two bases, two uprights and one horizontal bar. The two uprights are respectively installed on the two bases, and the two uprights are provided with several corresponding through third mounting holes. The two ends of the horizontal bar pass through the corresponding third mounting holes on the two uprights.
[0007] To allow for easy adjustment of the distance between adjacent horizontal bars according to the size of the tissue culture bag, several through fourth mounting holes are evenly distributed on the horizontal bars. The fourth mounting holes on the two horizontal bars of a pair of supports correspond to each other, and the two ends of the horizontal bars pass through the corresponding fourth mounting holes on the two horizontal bars. All horizontal bars are parallel and located at the same height.
[0008] In order to ensure that the opening of the tissue culture bag is large enough and remains stably open to facilitate the pouring of culture medium and inoculation, the first mounting hole on each side wall of the opposite side wall of the opening section of the tissue culture bag is two.
[0009] To prevent the first mounting hole on the opening section of the tissue culture bag from being accidentally torn by the downward pulling force generated when the culture medium solution is poured in, a reinforcing rib is provided along the edge of the first mounting hole.
[0010] Beneficial effects: The tissue culture bag flaring assembly of this utility model can open and fix the opening of the tissue culture bag by inserting the screw into the first mounting holes on both sides of the opening section of the tissue culture bag and the second mounting holes on the horizontal bars on both sides of the tissue culture bag. This allows only one person to pour the culture medium solution into the tissue culture bag, saving manpower. In addition, during the inoculation operation, the hands that were originally used to open the bag opening can be freed up to perform other operations, thereby improving efficiency. By setting several third mounting holes on the uprights, the height of the horizontal bars can be adjusted according to the size of the tissue culture bag, and the horizontal bars can be adjusted to a suitable height to facilitate opening the opening. By setting several fourth mounting holes on the crossbar, the distance between two adjacent horizontal bars can be adjusted, thereby allowing the opening to be widened to a suitable extent to facilitate the pouring of culture medium and inoculation. By setting two first mounting holes on each side wall of the opposite side wall of the tissue culture bag opening section, the opening of the tissue culture bag can be widened sufficiently after installation and can be stably kept in the open state, facilitating the pouring of culture medium and inoculation. By providing a reinforcing rib along the edge of the first mounting hole, the first mounting hole on the opening section of the tissue culture bag is prevented from being accidentally torn by the downward pulling force generated when the culture medium is poured in. Attached Figure Description
[0011] Figure 1 This is a schematic diagram of the structure of the tissue culture bag flaring component of this utility model;
[0012] In the diagram, 1 is the tissue culture bag; 2 is the first mounting hole; 3 is the base; 4 is the upright; 5 is the crossbar; 6 is the horizontal bar; 7 is the second mounting hole; 8 is the bolt; and 9 is the fourth mounting hole. Detailed Implementation
[0013] The technical solution of this utility model will be described in detail below through embodiments, but the protection scope of this utility model is not limited to the embodiments described.
[0014] like Figure 1 As shown, a tissue culture bag flaring assembly for tissue culture of *Anoectochilus roxburghii* includes a tissue culture bag 1, a pair of supports, several horizontal rods 6 connected between the pair of supports, and several bolts 8. The tissue culture bag 1 is a self-sealing bag with a self-sealing part, which divides the bag body into a lower body section and an upper open section. Two through first mounting holes 2 are provided on each of the opposite side walls of the open section. The two first mounting holes 2 on each side wall are located at opposite ends of the side wall. A reinforcing rib is provided along the edge of the first mounting hole 2 (i.e., thickening the edge of the self-sealing bag, not shown). Several through second mounting holes 7 are evenly distributed on the horizontal rods 6. The diameters of both the first mounting holes 2 and the second mounting holes 7 are larger than the diameter of the bolt 8's screw and smaller than the diameter of the bolt 8's head. The length of the screw extends from the top to the bottom of the second mounting holes 7.
[0015] Each bracket includes two bases 3, two uprights 4, and one horizontal bar 5. All three components are made of stainless steel. The bottoms of the two uprights 4 are welded to the two bases 3. Each upright has several corresponding through-holes in the third mounting holes. The two ends of the horizontal bar 5 pass through the corresponding third mounting holes on the two uprights 4. Several through-holes 9 are evenly distributed on the horizontal bar 5. The fourth mounting holes 9 on the two horizontal bars 5 of a pair of brackets correspond to each other. The two ends of the horizontal bars 6 pass through the corresponding fourth mounting holes 9 on the two horizontal bars 5. All horizontal bars 6 are parallel and at the same height.
[0016] The distance between two adjacent second mounting holes 7, the distance between two adjacent third mounting holes, and the distance between two adjacent fourth mounting holes 9 are all 2-5cm, for example, all can be 2cm.
[0017] In practical use, first adjust the height of the horizontal bar 5 and the spacing between adjacent horizontal bars 6 to suit the size of the tissue culture bag 1. Then, place the tissue culture bag 1 between two adjacent horizontal bars 6. Next, use bolts 8 to pass the screw through the first mounting hole 2 on one side wall of the opening section of the tissue culture bag and through the second mounting hole 7 on one horizontal bar 6, thus fixing one side wall of the opening section of the tissue culture bag. Similarly, fix the other side wall of the opening section of the tissue culture bag. In this way, the opening of the tissue culture bag 1 is opened and fixed, making it easy to pour in the culture medium solution and to inoculate *Anoectochilus roxburghii* into the culture medium. The height of the horizontal bar 5 and the spacing between adjacent horizontal bars 6 can be adjusted according to the size of the tissue culture bag 1. In addition, multiple horizontal bars 6 can be set up to fix multiple tissue culture bags 1 at the same time, realizing the filling of culture medium solution and inoculation of *Anoectochilus roxburghii* into multiple tissue culture bags 1 at one time. As for sealing and high-temperature sterilization after pouring in the culture medium solution, as well as subsequent inoculation of *Anoectochilus roxburghii* and maintenance, all refer to existing technologies.
[0018] Unless otherwise specified, all technologies mentioned above refer to existing technologies.
[0019] As described above, although the present invention has been shown and described with reference to specific preferred embodiments, it should not be construed as limiting the present invention itself. Various changes in form and detail may be made to the present invention without departing from the spirit and scope of the appended claims.
Claims
1. A flared-mouth component for tissue culture bags used in *Anoectochilus roxburghii* tissue culture, characterized in that, The device includes a tissue culture bag, a pair of supports, several horizontal bars connected between the pair of supports, and several bolts. The tissue culture bag is a self-sealing bag with a self-sealing part, which divides the bag body into a lower body part and an upper opening section. At least one through first mounting hole is provided on each of the opposite side walls of the opening section. Several through second mounting holes are evenly distributed on the horizontal bars. The diameters of the first and second mounting holes are both larger than the bolt screw diameter and smaller than the bolt head diameter.
2. The tissue culture bag flaring assembly for *Anoectochilus roxburghii* tissue culture according to claim 1, characterized in that, Each bracket includes two bases, two uprights and one crossbar. The two uprights are respectively installed on the two bases, and the two uprights are provided with several corresponding through third mounting holes. The two ends of the crossbar pass through the corresponding third mounting holes on the two uprights.
3. The tissue culture bag flaring assembly for *Anoectochilus roxburghii* tissue culture according to claim 2, characterized in that, Several through fourth mounting holes are evenly distributed on the crossbars. The fourth mounting holes on the two crossbars of a pair of brackets correspond to each other. The two ends of the horizontal bar pass through the corresponding fourth mounting holes on the two crossbars. All horizontal bars are parallel and located at the same height.
4. The tissue culture bag flaring assembly for *Anoectochilus roxburghii* tissue culture according to claim 3, characterized in that, There are two first mounting holes on each of the opposite side walls of the open section of the tissue culture bag.
5. The tissue culture bag flaring assembly for *Anoectochilus roxburghii* tissue culture according to claim 4, characterized in that, A reinforcing rib is provided along the edge of the first mounting hole.