Filtering auxiliary equipment for biopharmacy experiment

Abstract

The utility model discloses filtering auxiliary equipment for biopharmacy experiments, and relates to the technical field of biopharmacy. According to the technical key points, the device comprises a containing vessel, a balance weight base is placed in the center of the bottom of the containing vessel, a vertical insertion barrel is fixedly connected to the center of the upper side of the balance weight base, and a mounting shaft is movably inserted into the insertion barrel and can freely rotate around the axis of the insertion barrel in the insertion barrel; the upper end of the mounting shaft is fixedly sleeved with a first horizontal frame, and a plurality of through holes are evenly formed in the first horizontal frame in the circumferential direction. The upper end of the mounting shaft is fixedly connected with a rotating handle, and the height of the mounting shaft meets the requirement that the rotating handle can be located on the outer side of the upper end of the containing vessel when the mounting shaft is used. The dialysis bags can be driven to stir dialysate in the containing vessel by rotating the handle, so that the separation efficiency of small molecular substances in the dialysis bags is effectively improved, and the progress of a dialysis experiment is accelerated.

Description

Technical Field

[0001] This application relates to the field of biopharmaceutical technology, and in particular to a filtration auxiliary device for biopharmaceutical experiments. Background Technology

[0002] In biopharmaceutical manufacturing processes, filtration technology is one of the most widely used separation methods. In biopharmaceutical experiments, membrane separation technology is often used to separate specific substances in order to obtain them. Membrane separation technology utilizes the selectivity of membranes, using the energy difference across the membrane as the driving force, allowing certain components to permeate through the membrane while retaining other components in the mixture, thus achieving separation. In biopharmaceutical experiments, membranes in membrane separation technology can concentrate products, remove impurities, separate mixtures, and promote reactions. When using biomembranes for dialysis experiments, the common method is to fill a graduated cylinder with dialysate or purified water, and then suspend a dialysis bag containing the mixture inside the graduated cylinder by a cotton thread. The dialysate in the graduated cylinder is replaced periodically by the experimenter until small molecules in the mixture are removed. Since the dialysis bag mainly transports small molecules into the dialysate through natural diffusion within the graduated cylinder, and the rate of natural diffusion is relatively slow, this reduces the filtration efficiency of the mixture within the dialysis bag. Utility Model Content

[0003] This application provides a filtration auxiliary device for biopharmaceutical experiments, which can effectively improve the filtration efficiency of mixed substances in dialysis experiments.

[0004] The above-mentioned objective of this application is achieved through the following technical solution:

[0005] A filtration auxiliary device for biopharmaceutical experiments includes a container, a counterweight base placed at the center of the bottom of the container, a vertical insert fixedly connected to the center of the upper side of the counterweight base, and an installation shaft movably inserted into the insert, the installation shaft being able to rotate freely around its axis within the insert.

[0006] The upper end of the mounting shaft is fixedly fitted with a first horizontal frame, and the first horizontal frame is provided with multiple through holes evenly distributed along the circumference.

[0007] A rotating handle is fixedly connected to the upper end of the mounting shaft, and the height of the mounting shaft is such that the rotating handle is located on the outside of the upper end of the container when in use.

[0008] Furthermore, the upper side of the first horizontal frame is uniformly provided with sealing clips in the circumferential direction, the number of which is equal to the number of the plurality of through holes, and the positions of the plurality of sealing clips on the first horizontal frame correspond one-to-one with the positions of the plurality of through holes.

[0009] Furthermore, the sealing clip is a spring clip, the closed end of which is fixedly connected to the first horizontal frame via a fixing post, and the open end of which extends from the through hole near the mounting shaft to the outside of the first horizontal frame.

[0010] Furthermore, a limiting sleeve with the same inner diameter is coaxially provided above the insert, and the limiting sleeve and the edge of the insert are connected by two mutually symmetrical side posts.

[0011] Furthermore, a second horizontal frame is also installed on the mounting shaft below the first horizontal frame. The second horizontal frame is also provided with a plurality of the through holes and the sealing clips, and the through holes and the sealing clips on the second horizontal frame are in the same position and number as the through holes and the sealing clips on the first horizontal frame.

[0012] Furthermore, the second horizontal frame has a through clearance hole at its center. The diameter of the clearance hole is larger than the outer diameter of the limiting sleeve. The upper side of the clearance hole is connected to the mounting shaft by multiple F-shaped rods.

[0013] Furthermore, a drain tap is installed on one side of the bottom of the container.

[0014] In summary, this application includes at least one of the following beneficial technical effects:

[0015] Each through-hole on the first horizontal frame of this application can be fixed with a dialysis bag containing the mixture to be separated. After the mounting shaft on the first horizontal frame is inserted into the tube located on the counterweight base inside the container, the experimenter can drive the mounting shaft to rotate multiple dialysis bags on the first horizontal frame in the container by turning the handle. During this process, the dialysis bags can agitate the pre-prepared dialysis solution in the container, so that the small molecules precipitated in the dialysis bags can be quickly and evenly distributed in the dialysis solution. Compared with placing the dialysis bags in the dialysis solution, this can effectively improve the precipitation efficiency of small molecules in the dialysis bags, thereby speeding up the experimental progress. Attached Figure Description

[0016] To more clearly illustrate the technical solutions in the embodiments of this application or the prior art, the drawings used in the description of the embodiments or the prior art will be briefly introduced below. Obviously, the drawings described below are some embodiments of this application. For those skilled in the art, other drawings can be obtained based on these drawings without creative effort.

[0017] Figure 1 This is a schematic diagram of the overall structure of this application;

[0018] Figure 2 This is a schematic diagram of the internal structure of the container in this application;

[0019] Figure 3 This is a top view of the internal structure of the container in this application;

[0020] Figure 4 This is a schematic diagram showing the state after the mounting shaft of this application has been removed from the limiting sleeve and the insert.

[0021] Reference numerals in the attached drawings: 1. Container; 2. Counterweight base; 3. Insert; 4. Mounting shaft; 5. First horizontal frame; 6. Through hole; 7. Rotating handle; 8. Sealing clamp; 9. Fixing post; 10. Limiting sleeve; 11. Side post; 12. Second horizontal frame; 13. Clearance hole; 14. F-shaped hanging rod; 15. Drain tap. Detailed Implementation

[0022] To make the objectives, technical solutions, and advantages of the embodiments of this application clearer, the technical solutions in the embodiments of this application are described clearly and completely below. Obviously, the described embodiments are only some embodiments of this application, not all embodiments. Based on the embodiments in this application, all other embodiments obtained by those skilled in the art without creative effort are also within the scope of protection of this application.

[0023] like Figures 1-4 As shown, this application discloses a filtration auxiliary device for biopharmaceutical experiments, including a container 1. A counterweight base 2 is placed at the center of the bottom of the container 1. A vertical insert 3 is fixedly connected to the center of the upper side of the counterweight base 2. An installation shaft 4 is movably inserted into the insert 3 and can rotate freely around its axis. A first horizontal frame 5 is fixedly sleeved on the upper end of the installation shaft 4. The first horizontal frame 5 has multiple through holes 6 evenly distributed along its circumference. A rotating handle 7 is fixedly connected to the upper end of the installation shaft 4, and the height of the installation shaft 4 is such that the rotating handle 7 is located on the outside of the upper end of the container 1 during use.

[0024] In the above embodiments, the container 1 of this application can be a transparent glass cylinder, which allows the experimenter to easily observe the internal condition of the container 1 in real time during use. The counterweight base 2 of this application lowers the center of gravity of the entire mounting shaft 4 during use, thereby improving its stability during operation. The insert 3 on the counterweight base 2 is used to place the mounting shaft 4 and provides lateral support to the mounting shaft 4, ensuring that the mounting shaft 4 will not tip over after being placed in the insert 3.

[0025] During the dialysis experiment, the staff can first fix the prepared dialysis bags at the multiple through holes 6 of the first horizontal frame 5, insert the mounting shaft 4 into the insert 3 of the counterweight base 2 placed in the container 1, and then inject dialysis fluid into the container 1 until the dialysis fluid covers the dialysis bags. Then, the operator can hold the rotating handle 7 to drive the mounting shaft 4 to rotate around its own axis. The mounting shaft 4 can rotate together with multiple dialysis bags fixed on it via the first horizontal frame 5. During this process, the dialysis bags can agitate the pre-prepared dialysis solution in the container 1, so that the small molecules precipitated in the dialysis bags can be quickly and evenly distributed in the dialysis solution. In the existing technology, the dialysis bags are placed statically in the dialysis solution. Since it mainly relies on natural diffusion to discharge small molecules into the dialysis solution, the concentration of the solution near the dialysis bag will increase due to the untimely diffusion of small molecules in the static dialysis solution, which reduces the pressure difference inside and outside the dialysis bag and affects the precipitation of small molecules inside the dialysis bag. However, this application can agitate the dialysis solution by rotating multiple dialysis bags, thereby ensuring that the pressure difference inside and outside the dialysis bags can be maintained continuously and efficiently to precipitate small molecules inside the dialysis bags, effectively improving the precipitation efficiency of small molecules in the dialysis bags, thereby accelerating the experimental progress.

[0026] Furthermore, such as Figures 1-3 As shown, the upper side of the first horizontal frame 5 is uniformly provided with sealing clips 8 in the circumferential direction, the number of which is equal to the number of through holes 6, and the positions of the multiple sealing clips 8 on the first horizontal frame 5 correspond one-to-one with the positions of the multiple through holes 6.

[0027] In the above embodiments, a sealing clip 8 is installed at each through hole 6 on the first horizontal frame 5. This allows the experimenter to quickly fix the dialysis bag in place by using the sealing clip 8 after inserting the dialysis bag into the through hole 6, thereby improving the convenience of operation.

[0028] Furthermore, such as Figures 1-3 As shown, the sealing clip 8 is a spring clip. The closed end of the spring clip is fixedly connected to the first horizontal frame 5 through the fixing post 9, and the open end of the spring clip extends from the through hole 6 near the mounting shaft 4 to the outside of the first horizontal frame 5.

[0029] In the above embodiments, the sealing clip 8 of this application is a spring clip. Its closed end is fixedly installed on the upper end of the through hole 6 at the corresponding position near the mounting shaft 4 by the fixing post 9, and its open end is located on the upper end of the through hole 6 at the corresponding position away from the mounting shaft 4. This makes it convenient for the experimenter to pry open the spring clip from the open end. After placing one section of the dialysis bag into the spring clip, the experimenter releases the spring clip, and the spring clip can quickly close under the action of elasticity to automatically complete the fixing of the dialysis bag. When the dialysis bag needs to be removed later, the experimenter only needs to pry open the spring clip again, which is simple and convenient to use.

[0030] Furthermore, such asFigure 2 and Figure 4 As shown, a limiting sleeve 10 with the same inner diameter is coaxially arranged above the insert 3. The limiting sleeve 10 and the edge of the insert 3 are connected by two mutually symmetrical side posts 11.

[0031] In the above embodiments, when the mounting shaft 4 is inserted into the insert 3, it will first pass through the limiting sleeve 10 set at intervals directly above the insert 3. This reduces the suspended section of the mounting shaft 4 during use, thereby improving the stability of its rotation process.

[0032] Furthermore, such as Figures 2-4 As shown, a second horizontal frame 12 is also installed on the mounting shaft 4 below the first horizontal frame 5. The second horizontal frame 12 is also provided with multiple through holes 6 and sealing clips 8, and the through holes 6 and sealing clips 8 on the second horizontal frame 12 are in the same position and number as the through holes 6 and sealing clips 8 on the first horizontal frame 5.

[0033] In the above embodiments, the second horizontal frame 12 provided below the mounting shaft 4 is provided with the same number and position of through holes 6 and sealing clips 8 as the first horizontal frame 5. In this way, when the dialysis bag is installed, its upper and lower ends can be fixed in the through holes 6 at corresponding positions on the first horizontal frame 5 and the second horizontal frame 12 by the sealing clips 8. When the dialysis bag rotates rapidly with the mounting shaft 4, it can prevent the lower end of the dialysis bag from being thrown up under the action of centrifugal force and colliding with the inner wall of the container 1, causing damage to the dialysis bag.

[0034] Furthermore, such as Figure 2 and Figure 4 As shown, the center of the second horizontal frame 12 is provided with a through clearance hole 13. The diameter of the clearance hole 13 is larger than the outer diameter of the limiting sleeve 10. The upper side of the clearance hole 13 is connected to the mounting shaft 4 by multiple F-shaped hangers 14.

[0035] In the above embodiments, the clearance hole 13 provided at the center of the second horizontal frame 12 of this application has a diameter larger than that of the limiting sleeve 10. This facilitates the smooth passage of the second horizontal frame 12 through the limiting sleeve 10 when the mounting shaft 4 is passed through it. The upper side of the second horizontal frame 12 is connected to the mounting shaft 4 through the F-shaped hanger 14. The two horizontal bars of the F-shaped hanger 14 are fixedly connected to the mounting shaft 4, and its vertical bar is connected to the second horizontal frame 12. In this way, after the mounting shaft 4 is inserted into the insert 3, the F-shaped hanger 14 can provide a high support force to the second horizontal frame 12 through the mounting shaft 4, while also flexibly avoiding the interference of the limiting sleeve 10.

[0036] Furthermore, such as Figure 1 and Figure 2 As shown, a drain tap 15 is installed on one side of the bottom of the container 1.

[0037] In the above embodiments, since the dialysate in container 1 needs to be changed multiple times during the dialysis experiment, if the experimenter had to lift container 1 to empty the used dialysate each time, the weight of container 1 plus the weight of the dialysate would make the operation very strenuous. Therefore, this application provides a drain tap 15 on one side of the bottom of container 1. This way, when dialysate needs to be drained during the experiment, the experimenter can connect a drain tube to the drain tap 15 and then open the drain tap 15 to quickly drain the used dialysate from container 1, effectively improving the convenience of dialysate replacement.

[0038] The implementation principle of this embodiment is as follows: During the dialysis experiment, the operator first fixes the prepared dialysis bags sequentially into the multiple through holes 6 of the first horizontal frame 5 and the second horizontal frame 12. The mounting shaft 4 is inserted into the insert 3 of the counterweight base 2 placed in the container 1. Next, dialysis fluid is injected into the container 1 until the dialysis fluid covers the dialysis bags. Then, the operator can hold the rotating handle 7 to drive the mounting shaft 4 to rotate around its own axis. The mounting shaft 4 can rotate together with the multiple dialysis bags fixed on it through the first horizontal frame 5. During this process, the dialysis bags can agitate the pre-prepared dialysis fluid in the container 1, so that the small molecules precipitated in the dialysis bags can be quickly and evenly distributed in the dialysis fluid. Compared with the prior art, this application uses the rotation of multiple dialysis bags to agitate the dialysis fluid, thereby ensuring that the pressure difference inside and outside the dialysis bags can be maintained continuously and efficiently to precipitate the small molecules in the dialysis bags, effectively improving the precipitation efficiency of small molecules in the dialysis bags, thereby accelerating the experimental progress.

[0039] Finally, it should be noted that the above embodiments are only used to illustrate the technical solutions of this application, and are not intended to limit them. Although this application has been described in detail with reference to the foregoing embodiments, those skilled in the art should understand that modifications can still be made to the technical solutions described in the foregoing embodiments, or equivalent substitutions can be made to some or all of the technical features therein. Such modifications or substitutions do not cause the essence of the corresponding technical solutions to deviate from the scope of the technical solutions of the embodiments of this application.

Claims

1. A filtration auxiliary device for biopharmaceutical experiments, characterized in that: The container includes a holding vessel (1), a counterweight base (2) is placed at the center of the bottom of the holding vessel (1), a vertical insert (3) is fixedly connected to the center of the upper side of the counterweight base (2), and an installation shaft (4) is movably inserted into the insert (3), and the installation shaft (4) can rotate freely around its axis in the insert (3). The upper end of the mounting shaft (4) is fixedly fitted with a first horizontal frame (5), and the first horizontal frame (5) is provided with a plurality of through holes (6) evenly distributed along the circumference. A rotating handle (7) is fixedly connected to the upper end of the mounting shaft (4), and the height of the mounting shaft (4) is such that the rotating handle (7) can be located on the outside of the upper end of the container (1) when in use.

2. The filtration auxiliary device for biopharmaceutical experiments according to claim 1, characterized in that: The upper side of the first horizontal frame (5) is uniformly provided with sealing clips (8) in the same number as the plurality of through holes (6) along the circumferential direction, and the positions of the plurality of sealing clips (8) on the first horizontal frame (5) correspond one-to-one with the positions of the plurality of through holes (6).

3. The filtration auxiliary device for biopharmaceutical experiments according to claim 2, characterized in that: The sealing clip (8) is a spring clip. The closed end of the spring clip is fixedly connected to the first horizontal frame (5) through the fixing post (9). The open end of the spring clip extends from the through hole (6) near the mounting shaft (4) to the outside of the first horizontal frame (5).

4. The filtration auxiliary device for biopharmaceutical experiments according to claim 3, characterized in that: A limiting sleeve (10) with the same inner diameter is provided coaxially above the insert (3). The limiting sleeve (10) and the edge of the insert (3) are connected by two mutually symmetrical side posts (11).

5. The filtration auxiliary device for biopharmaceutical experiments according to claim 4, characterized in that: A second horizontal frame (12) is also installed on the mounting shaft (4) below the first horizontal frame (5). The second horizontal frame (12) is also provided with a plurality of through holes (6) and sealing clips (8). The through holes (6) and sealing clips (8) on the second horizontal frame (12) are in the same position and number as the through holes (6) and sealing clips (8) on the first horizontal frame (5).

6. The filtration auxiliary device for biopharmaceutical experiments according to claim 5, characterized in that: The second horizontal frame (12) has a through hole (6) at its center. The diameter of the through hole (6) is larger than the outer diameter of the limiting sleeve (10). The upper side of the edge of the through hole (6) is connected to the mounting shaft (4) by multiple F-shaped rods (14).

7. The filtration auxiliary device for biopharmaceutical experiments according to any one of claims 1 to 6, characterized in that: A drain tap (15) is installed on one side of the bottom of the container (1).

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