Sample freezing device for immunohistochemical detection
By designing the pushing components and sliding rod system inside the ice bath, the problems of low sample freezing efficiency and inconvenient operation in the existing technology are solved, enabling simultaneous freezing and convenient removal of multiple samples, thus improving operational efficiency and stability in the low-temperature environment.
Patent Information
- Application Number
- CN202520264158.3
- Authority / Receiving Office
- CN · China
- Patent Type
- Utility models(China)
- Current Assignee / Owner
- Filing Date
- 2025-02-19
- Publication Date
- 2025-12-16
- Estimated Expiration
- 2035-02-19
AI Technical Summary
Existing cryopreservation devices for immune tissue samples are inefficient and inconvenient to operate, especially as the evaporation of the cryoprotectant at low temperatures leads to obscured visibility and makes it difficult to retrieve sample trays.
An ice bath chamber was designed, which includes an ice bath cavity and a structural cavity. Multiple samples in the sample tray can be frozen simultaneously and easily removed by pushing components and a sliding rod system. The design of the damping slider and the lid ensures the stability of the low-temperature environment and the convenience of operation.
It improves sample freezing efficiency, enables simultaneous freezing of multiple samples and facilitates convenient operation, and ensures the stability of the low-temperature environment and easy removal of sample trays.
Smart Images
Figure CN223678926U_ABST
Abstract
Description
TECHNICAL FIELD
[0001] The utility model relates to biological experiment equipment field especially, and relates to a sample refrigerating device for immunohistochemistry detection. BACKGROUND
[0002] The sample for immunohistochemistry detection is mainly the tissue section after specific treatment, and the principle of specific binding of immunology antigen and antibody is applied to make the color developing agent (fluorescein, enzyme, metal ion, isotope) of marked antibody color through chemical reaction, determine the antigen (polypeptide and protein) in tissue or cell, and carry out positioning, qualitative and relative quantitative inspection.
[0003] The acquisition of tissue section needs to freeze and embed the immunohistochemistry sample and then slice it, and the current freezing mode of immunohistochemistry sample is to put the sample in a sample holder and ice bath the sample holder in low-temperature refrigerant to realize rapid freezing. However, such operation mode can only freeze one sample at a time, and the evaporation of low-temperature refrigerant at normal temperature will generate a large amount of steam at the bottom of the ice bath container, which is difficult to see, and the sample holder is inconvenient to take. Therefore, a sample refrigerating device for immunohistochemistry detection is proposed. SUMMARY
[0004] The utility model discloses a sample refrigerating device for immunohistochemistry detection, and solves the problems of low freezing efficiency and inconvenient operation of the existing immunohistochemistry sample.
[0005] To achieve the above-mentioned purpose, the utility model provides the following technical scheme: a sample refrigerating device for immunohistochemistry detection, including ice bath box, the both sides in ice bath box are provided with ice bath cavity, and the middle is provided with structure cavity, the sliding connection of ice bath cavity is provided with the frame board, be connected with the support frame on the frame board, the support frame is supported with the sample holder, the both sides inner wall of structure cavity all are connected with the slide rod of sliding, and the extension end of ice bath box outside is connected with the supporting plate, and each supporting plate all slidingly penetrates ice bath box and extends in the ice bath cavity, the sliding connection of structure cavity is penetrated and is provided with the pull frame, be provided with the pusher assembly on the pull frame, the pusher assembly is used for pulling the slide rod of both sides to be close to each other or away from each other, to pull the supporting plate of the lower end of sample holder.
[0006] Preferably, the pusher assembly includes a guide slide rod, a rotating plate, and a spring. The guide slide rod is connected to the inner walls of the structure cavity, and the ends of the guide slide rod are symmetrically sleeved with slide plates. The slide rods on both sides are connected to the slide plates on the side away from each other. The ends of the rotating plate are rotatably connected to the slide plates and the pull frame. The spring is sleeved outside the guide slide rod, and the ends of the spring are connected to the inner walls of the structure cavity and the slide plates.
[0007] Preferably, the two side inner walls of the ice bath cavity are connected with slides, and the slides are connected with sliding blocks in a damping manner, the sliding blocks are connected with the shelf plate, the support frame is provided with clamping grooves, the sample box is clamped in the clamping grooves in correspondence, and the shelf plate is connected with a pull rod.
[0008] Preferably, the extension ends of the two slides outside the ice bath box are connected with connecting plates, the shelf plates are arranged in plurality and connected on the connecting plates, and the ice bath box is provided with slide holes penetrating the side surface.
[0009] Preferably, the two sides of the ice bath box are connected with slide rails, the slide rails are connected with box covers in a damping and sliding manner, and the two box covers are respectively combined with the two ice bath cavities.
[0010] Preferably, the slide holes are arranged on the slide rails, the two sides of the box cover are provided with limiting holes penetrating the two sides, and the shelf plates are inserted in the limiting holes in cooperation.
[0011] Compared with the related art, the sample freezing device for immunohistochemical detection has the following beneficial effects:
[0012] The sample freezing device for immunohistochemical detection has the following beneficial effects: BRIEF DESCRIPTION OF DRAWINGS
[0013] Figure 1 It is a structural schematic view of the utility model.
[0014] Figure 2 It is a sectional view of the structure of the utility model in side view.
[0015] Figure 3 It is a structural schematic view of the internal structure of the ice bath box.
[0016] Figure 4 It is a structural schematic view of the internal structure of the ice bath cavity.
[0017] Figure 5 It is a sectional view of the structure of the utility model in side view.
[0018] In the figure: 1, ice bath box; 2, ice bath cavity; 3, structure cavity; 4, slide rail; 5, box cover; 6, limiting hole; 7, slide hole; 8, slide; 9, sliding block; 10, shelf plate; 11, support frame; 12, clamping groove; 13, sample storage box; 14, guide slide rod; 15, slide plate; 16, slide rod; 17, connecting plate; 18, supporting plate; 19, pull frame; 20, rotating plate; 21, spring; 22, pull rod. DETAILED DESCRIPTION
[0019] The technical solutions in the embodiments of the utility model will be clearly and completely described below with reference to the drawings in the embodiments of the utility model. Obviously, the described embodiments are only part of the embodiments of the utility model, rather than all the embodiments. Based on the embodiments in the utility model, all other embodiments obtained by those skilled in the art without creative labor fall within the scope of the utility model.
[0020] Please refer to Figures 1-5 The utility model provides a kind of technical scheme: a sample freezing device for immunohistochemical detection, including ice bath box 1, ice bath box 1 inside two sides are provided with ice bath cavity 2, middle is provided with structure cavity 3, shelf plate 10 is slidably connected in ice bath cavity 2, and shelf plate 10 is connected with support frame 11, and support frame 11 is supported with sample storage box 13, and the both sides inner wall of structure cavity 3 is slidably connected with slide rod 16, and is connected with supporting plate 18 in the extension end outside ice bath box 1, and each supporting plate 18 is slidably connected with ice bath box 1 and extends in ice bath cavity 2, and slide rod 16 is slidably connected in structure cavity 3, and pull frame 19 is provided with pusher assembly on pull frame 19, and pusher assembly is used to pull the slide rod 16 of two sides to be close to each other or away from each other, to pull supporting plate 18 and support the lower end of sample storage box 13.
[0021] Further pusher assembly includes guide slide rod 14, rotating plate 20, spring 21, guide slide rod 14 is connected in the both sides inner wall of structure cavity 3, and the both ends of guide slide rod 14 are respectively symmetrically slidably sleeved with slide plate 15, and the slide rod 16 of two sides is respectively connected in the slide plate 15 of two sides and is away from each other, and the both ends of rotating plate 20 are respectively rotationally connected on slide plate 15 and pull frame 19, and spring 21 is sleeved outside guide slide rod 14, and the both ends of spring 21 are respectively connected on the inner wall of structure cavity 3 and slide plate 15, as Figure 2 As shown, by pressing down pull frame 19, drive both sides rotating plate 20 to rotate, to push both sides slide plate 15 and be away from each other on guide slide rod 14, and compress spring 21, drive the slide rod 16 of two sides to be away from each other, push both sides supporting plate 18 also be away from each other, then when pull frame 22 is released, under the elastic force of spring 21, both sides slide plate 15 will be close to each other again, and drive supporting plate 18 and pull frame 19 to reset.
[0022] Further, the inner walls on both sides of the ice bath chamber 2 are connected with slide ways 8, and the slide ways 8 are connected with sliding blocks 9 in a damping sliding mode, the sliding blocks 9 are connected with the shelf plates 10, the supporting frames 11 are provided with clamping grooves 12, and the sample holding boxes 13 are clamped in the clamping grooves 12 correspondingly, the shelf plates 10 are connected with pull rods 22, as shown in Figure 1 and 4 The sliding blocks 9 are connected with the shelf plates 10 in a damping sliding mode in the slide ways 8, so that the shelf plates 10 can be moved stably on the upper side or the lower side of the ice bath chamber 2 through the damping sliding connection, and the supporting frames 11 and the sample holding boxes 13 supported by the clamping grooves 12 on the supporting frames 11 can be moved stably on the upper side or the lower side of the ice bath chamber 2 respectively, so as to realize the ice bath with the low-temperature solution in the ice bath chamber 2 when the sample holding boxes 13 are moved to the lower side of the ice bath chamber 2, and the sample holding boxes 13 are convenient to take out when the sample holding boxes 13 are moved to the upper side of the ice bath chamber 2, the clamping grooves 12 are convenient for the stable placement of the sample holding boxes 13 on the supporting frames 11, and the pull rods 22 are convenient for the lifting of the shelf plates 10.
[0023] Further, the extension ends of the slide rods 16 on both sides are connected with connecting plates 17 outside the ice bath box 1, the supporting plates 18 are provided in plurality and connected with the connecting plates 17, the slide holes 7 are provided through the sides of the ice bath box 1, and the supporting plates 18 slide through the slide holes 7 and extend into the ice bath chamber 2, as shown in Figure 3 When one slide rod 16 slides, the connecting plate 17 and the plurality of supporting plates 18 connected with the connecting plate 17 can slide in the slide hole 7, so that the supporting plates 18 can slide into the ice bath chamber 2 or outside the ice bath box 1 respectively.
[0024] Further, the ice bath box 1 is connected with slide rails 4 on both sides, the slide rails 4 are connected with box covers 5 in a damping sliding mode, and the box covers 5 on both sides are covered with the ice bath chambers 2 on both sides, as shown in Figure 1 and 3 The box covers 5 are convenient for the ice bath operation to be insulated when the sample holding boxes 13 are moved to the bottom of the ice bath chamber 2 for ice bath, and the efficiency of the ice bath operation is improved.
[0025] Further, the slide holes 7 are provided on the slide rails 4, the box covers 5 are provided with limiting holes 6 through the sides, and the supporting plates 18 are inserted into the limiting holes 6 in cooperation, as shown in Figure 1 and 3As shown, in specific use, first press down the pull frame 19, drive the two sides of the supporting plate 18 away from each other to the outside of the ice bath cavity 2, through the pull rod 22 to lift the frame plate 10 to the upper of the ice bath cavity 2, at this time, loosen the pull frame 19 under the action of the spring, the pull frame 19 moves up to reset, the sample box 13 containing samples are placed in the clamping groove 12 respectively, again press down the pull frame 19 and drive the two sides of the supporting plate 18 away from each other to the outside of the ice bath cavity 2, through the pull rod 22 to press down the frame plate 10 to the frozen solvent in the ice bath cavity 2 to carry on the ice bath, at the same time, the box cover 5 can be selectively slid, so that the box cover 5 can cover the ice bath cavity 2, to facilitate the ice bath process to keep warm, improve the ice bath efficiency, when selecting to close the box cover 5, after the ice bath is completed, first need to press down the pull frame 19, and slide the box cover 5 to open the upper of the ice bath cavity 2, then pull the pull rod 22 to drive the sample box 13 supported on the supporting frame 11 to move to the upper of the supporting plate 18, at this time, loosen the pull frame 19, under the action of the spring 21, the supporting plate 18 resets, at this time, directly press down the pull rod 22, the frame plate 10 and the supporting frame 11 move down, the sample box 13 on it can be automatically left on the supporting plate 18, facilitate the sample box 13 to be taken out, and one ice bath can freeze multiple samples, when ice bath, do not close the box cover 5, keep it open.
Claims
1. A sample freezing device for immunohistochemical detection, comprising an ice bath box (1), two ice bath cavities (2) are arranged on both sides of the ice bath box (1), and a structure cavity (3) is arranged in the middle, characterized in that, The ice bath cavity (2) is slidably connected with a shelf plate (10), the shelf plate (10) is connected with a support frame (11), the support frame (11) supports a sample holder box (13), the structure cavity (3) is slidably connected with a slide rod (16) penetrating through the inner wall of both sides, and the extension end of the ice bath box (1) is connected with a supporting plate (18), each supporting plate (18) is slidably connected with the ice bath box (1) and extends in the ice bath cavity (2), the structure cavity (3) is slidably connected with a pull frame (19) penetrating through the structure cavity (3), the pull frame (19) is provided with a pushing assembly, the pushing assembly is used for pulling the slide rods (16) on both sides to approach or away from each other, so as to pull the supporting plate (18) to support the lower end of the sample holder box (13).
2. The sample freezing device for immunohistochemical detection according to claim 1, wherein The pushing assembly comprises a guide slide rod (14), a rotating plate (20) and a spring (21), the guide slide rod (14) is connected to the inner wall of both sides of the structure cavity (3), and the both ends of the guide slide rod (14) are symmetrically slidably sleeved with a slide plate (15), the slide rods (16) on both sides are connected to the slide plates (15) on both sides away from each other, the both ends of the rotating plate (20) are rotatably connected to the slide plates (15) and the pull frame (19), and the spring (21) is sleeved outside the guide slide rod (14), and the both ends of the spring (21) are connected to the inner wall of the structure cavity (3) and the slide plate (15).
3. The sample freezing device for immunohistochemical detection according to claim 2, wherein The both sides of the ice bath cavity (2) are connected with a slide (8), and the slide (8) is connected with a slide block (9) in a damping sliding manner, the slide block (9) is connected with the shelf plate (10), the support frame (11) is provided with a clamping groove (12), and the sample holder box (13) is clamped in the clamping groove (12), and the shelf plate (10) is connected with a pull rod (22).
4. The sample freezing device for immunohistochemical detection according to claim 1, wherein The both sides of the slide rod (16) are connected with a connecting plate (17) at the extension end outside the ice bath box (1), the supporting plates (18) are provided in plurality and connected to the connecting plate (17), and the ice bath box (1) is provided with a slide hole (7) penetrating through the side surface, the supporting plates (18) are slidably connected with the slide hole (7) and extend in the ice bath cavity (2).
5. The sample freezing device for immunohistochemical detection according to claim 4, wherein The both sides of the ice bath box (1) are connected with slide rails (4), the slide rails (4) are connected with box covers (5) in a damping sliding manner, and the both sides of the box covers (5) are connected with the both sides of the ice bath cavity (2).
6. The sample freezing device for immunohistochemical detection according to claim 5, wherein The slide hole (7) is provided on the slide rail (4), the both sides of the box cover (5) are provided with limiting holes (6) penetrating through, and the supporting plates (18) are inserted in cooperation with the limiting holes (6).