Incubation box convenient for cleaning cell climbing slide
By designing inclined fixing blocks and partition structures in the incubation box, the problem of cell slide detachment and removal during the washing process was solved, achieving stable washing and efficient experiments.
Patent Information
- Application Number
- CN202423006390.1
- Authority / Receiving Office
- CN · China
- Patent Type
- Utility models(China)
- Current Assignee / Owner
- Filing Date
- 2024-12-05
- Publication Date
- 2025-12-19
- Estimated Expiration
- 2034-12-05
AI Technical Summary
Cell slides are prone to detachment during the washing process and are difficult to remove after washing, leading to experimental failures and wasted resources.
An incubation box was designed, which includes a fixed block and a partition set at an angle. After the cell slide is wetted by liquid, it is automatically fixed on the inclined slope. The washing solution is injected from the gap between the fixed block and the box body to avoid direct impact. After washing, it is slowly removed with tweezers.
This effectively prevents cell slides from detaching and breaking, improves experimental efficiency, reduces waste of experimental resources, and avoids cross-reaction effects.
Smart Images
Figure CN223692382U_ABST
Abstract
Description
Technical Field
[0001] This utility model relates to the field of experimental equipment technology, specifically to an incubation box that facilitates the cleaning of cell slides. Background Technology
[0002] Immunofluorescence staining is an experimental method that combines immunological techniques with fluorescent labeling techniques. It can visually demonstrate the localization and distribution characteristics of various target proteins in cellular structures, and is an important tool in molecular immunology research, playing a decisive role in disease diagnosis, studying cell structure and function, and analyzing protein expression. The simplified procedure is as follows: A coverslip with cells on one side (i.e., a cell smear) is placed in an incubation chamber. After cell membrane rupture, washing, and blocking, the target protein antibody is dropped onto the coverslip. Incubation is performed overnight at 4°C to promote a full immune reaction between the antibody and intracellular antigen. After washing, a fluorescently labeled secondary antibody is added, followed by incubation in the dark, washing, and finally photographing under a fluorescence microscope to complete the experiment.
[0003] The entire process of immunofluorescence staining involves multiple washing steps of the cell slides. During these washing processes, the cells covering the slides are very easy to detach. The commonly used washing method is to place the cell slides into a discarded six-well cell culture dish and pour washing buffer from above. This direct impact method easily causes cells to detach from the coverslip. At the same time, the liquid wetting at the bottom of the coverslip and the bottom of the culture dish creates a large negative pressure suction, making it difficult to remove the cells easily with tweezers. Repeated handling with tweezers or improper force can even cause the cell slides to break, leading to experimental failure and wasting biological samples and experimental funds. Utility Model Content
[0004] Therefore, this invention aims to solve the problems in the prior art where cells are easily detached due to impact during cleaning of cell slides, and the cell slides are difficult to remove after cleaning, thereby providing an incubation box that facilitates the cleaning of cell slides.
[0005] To solve the above-mentioned technical problems, the technical solution of this utility model is as follows:
[0006] An incubation box for easy cleaning of cell slides includes a box body and a fixing block disposed within the box body. One end of the fixing block is spaced apart from the side wall of the box body, and one side of the fixing block has an inclined slope. The cell slide is adapted to rest against the inclined slope, and the height of the inclined slope is less than the height of the cell slide.
[0007] Furthermore, the inclined slope is provided with strip grooves extending along its length.
[0008] Furthermore, the box body is provided with a plurality of fixing blocks fixed to the bottom of the box body.
[0009] Further, a plurality of partitions are arranged in the box body, and the box body is divided into a plurality of cleaning tanks with the same size by the partitions.
[0010] Further, a plurality of fixing blocks are arranged in the cleaning tank, and the fixing blocks are arranged side by side in the cleaning tank.
[0011] Further, one end of the fixing block is arranged apart from the partition or the side wall of the box body, and the other end of the fixing block is fixed to the side wall of the partition or the side wall of the box body.
[0012] Further, the interval between the two adjacent fixing blocks in the same cleaning tank is 10mm-20mm.
[0013] Further, the interval between the other end of the fixing block and the partition or the side wall of the box body is 10mm-20mm.
[0014] Further, the inclination of the inclined slope surface of the fixing block is 45 degrees-75 degrees.
[0015] Further, the height of the fixing block is 8mm-14mm.
[0016] The technical scheme of the utility model has the following advantages:
[0017] 1. The incubation box for cleaning cell climbing slices can automatically fix the cell climbing slices on the inclined slope surface of the fixing block in an adsorbed manner after the cell climbing slices are soaked in liquid, so that the cell climbing slices can be prevented from being damaged due to falling; since one end of the fixing block is arranged apart from the side wall of the box body, the cleaning liquid can be injected from the interval between the fixing block and the box body when the cell climbing slices are cleaned, so that the cell climbing slices can be prevented from being directly impacted by the cleaning liquid and the cells on the cell climbing slices can be prevented from falling off; since the height of the inclined surface of the inclined slope surface is smaller than the height of the cell climbing slices, the cell climbing slices can be slowly moved out by clamping the edge part that is not in contact with the inclined slope surface of the fixing block with tweezers after the cell climbing slices are cleaned, so that the cell climbing slices can be gradually separated from the inclined slope surface of the fixing block, and the cell climbing slices can be prevented from being broken due to repeated clamping with the tweezers or improper force.
[0018] 2. The incubation box for cleaning cell climbing slices is provided with a strip-shaped groove extending along the length direction of the inclined slope surface, so that the negative pressure between the strip-shaped groove and the cell climbing slices can be avoided, the overall negative pressure suction force between the cell climbing slices and the inclined slope surface of the fixing block can be reduced, the cell climbing slices can be more easily taken out, and the risk of violently clamping and breaking the cell climbing slices due to excessive adsorption force can be avoided.
[0019] 3. The incubation box for cleaning cell climbing slices, wherein the box body is internally provided with a plurality of fixing blocks fixed to the bottom of the box body, so that a plurality of cell climbing slices can be cleaned at the same time, the number of liquid adding times is reduced, and the experimental efficiency is improved.
[0020] 4. The incubation box for cleaning cell climbing slices, wherein a plurality of partitions are internally and intermittently arranged in the box body, the plurality of partitions divide a plurality of cleaning tanks with the same size in the box body, and each cleaning tank is internally provided with a fixing block. In this way, the experimental efficiency is improved, and cross-reactions of cell climbing slices between different experimental purposes are avoided to affect the experimental results. BRIEF DESCRIPTION OF DRAWINGS
[0021] In order to more clearly illustrate the technical scheme in the specific embodiments of the present application or the prior art, the drawings needed to be used in the specific embodiments or the prior art description will be briefly introduced. Obviously, the drawings in the following description are some embodiments of the present application, and other drawings can be obtained by those skilled in the art without creative labor on the basis of these drawings.
[0022] Fig. 1 The incubation box for cleaning cell climbing slices is provided with a perspective view of the incubation box for cleaning cell climbing slices.
[0023] Fig. 2 The incubation box for cleaning cell climbing slices is provided with a top view of the incubation box for cleaning cell climbing slices.
[0024] Fig. 3 The incubation box for cleaning cell climbing slices is provided with a side view of the incubation box for cleaning cell climbing slices.
[0025] The drawings are explained as follows: 1, box body; 2, box cover; 3, fixing block; 4, partition; 5, cell climbing slice; 6, inclined slope. DETAILED DESCRIPTION
[0026] The technical scheme of the present application will be described in detail below with reference to the drawings. Obviously, the described embodiments are some embodiments of the present application, but not all embodiments. Based on the embodiments in the present application, all other embodiments obtained by those skilled in the art without creative labor fall within the scope of the present application.
[0027] In the description of the utility model, it needs to explain, the term "center", "upper", "lower", "left", "right", "vertical", "horizontal", "internal", "external" and so on indicate the orientation or position relation based on the orientation or position relation shown in the drawing, only for the convenience of describing the utility model and simplifying the description, and not indicate or imply that the indicated device or element must have a particular orientation, construct and operate in a particular orientation, therefore, it cannot be understood as a limitation on the utility model. In addition, the terms "first", "second", "third" are only for the purpose of description, and cannot be understood as indicating or implying relative importance.
[0028] In the description of the utility model, it needs to explain, unless otherwise explicitly provided and limited, the terms "mounting", "connecting", "connection" should be understood broadly, for example, it can be fixed connection, or detachable connection, or integrally connected, it can be mechanical connection, or electrical connection, it can be directly connected, or indirectly connected through intermediate medium, it can be the communication inside two elements. For ordinary skilled in the art, the specific meaning of the above terms in the utility model can be understood according to the specific circumstances.
[0029] In addition, the technical features involved in different embodiments of the utility model described below can be combined with each other as long as there is no conflict.
[0030] As Figs. 1-3 The utility model discloses an incubation box convenient for cleaning cell climbing piece, including box body 1 and the fixed block 3 of setting in the box body 1, one end of the fixed block 3 is spaced apart with the lateral wall of the box body 1, one side of the fixed block 3 is the inclined slope 6 of inclined arrangement, cell climbing piece 5 is suitable for leaning on the inclined slope 6, and the height of the inclined surface of the inclined slope 6 is less than the height of cell climbing piece 5.
[0031] The incubation box convenient for cleaning cell climbing piece, cell climbing piece 5 can be automatically fixed on the inclined slope 6 of the fixed block 3 in the way of adsorption after being soaked by liquid, which can avoid damage caused by cell climbing piece 5 from falling down, the fixed block 3 is spaced apart with the lateral wall of the box body 1, and when cleaning cell climbing piece 5, the cleaning liquid can be injected from the interval between the fixed block 3 and the box body 1, so that the cell climbing piece 5 can be avoided from being directly impacted by the cleaning liquid, and the cells on the cell climbing piece 5 can be avoided from falling off, the height of the inclined surface of the inclined slope 6 is less than the height of cell climbing piece 5, after cleaning the cell climbing piece 5, the edge part of the cell climbing piece 5 not in contact with the inclined slope 6 of the fixed block 3 can be clamped by using tweezers, the cell climbing piece 5 is slowly moved out, the cell climbing piece 5 is gradually separated from the inclined slope 6 of the fixed block 3, and the cell climbing piece 5 can be avoided from being broken due to repeated use of tweezers or improper force.
[0032] In this embodiment, a lid 2 is rotatably provided on one side of the box body 1, suitable for flipping over and covering it. This arrangement allows the lid 2 to be closed after cleaning the cell slides 5, preventing dust from falling onto the cell slides 5 and affecting subsequent experimental results. Specifically, the width of the box body 1 is 168mm, the depth of the box body 1 is 120mm, and the height of the box body 1 is 30mm.
[0033] In this embodiment, multiple partitions 4 are spaced apart inside the box 1, dividing the box 1 into multiple washing tanks of the same size. Each washing tank is equipped with a fixing block 3. This arrangement improves experimental efficiency while preventing cross-reactions between cell smears 5 for different experimental purposes, thus avoiding interference with experimental results.
[0034] In this embodiment, a plurality of fixing blocks 3 are arranged side by side in the washing tank. Specifically, the distance between two adjacent fixing blocks 3 in the washing tank is 15 mm. This arrangement allows for the simultaneous washing of multiple cell slides 5, reducing the number of liquid additions and improving experimental efficiency.
[0035] Specifically, one end of the fixing block 3 is spaced 15mm from the partition 4 or the side wall of the box 1, forming an injection channel for the cleaning solution. The other end of the fixing block 3 is fixed to the side wall of the partition 4 or the side wall of the box 1. With this arrangement, during cleaning, the cleaning solution enters from the injection channel and then flows between the two spaced-apart fixing blocks, thus avoiding direct rinsing of the cell slides and preventing cell detachment from the cell slides.
[0036] Specifically, the slope 6 of the fixed block 3 has an inclination of 60 degrees, the width of the fixed block 3 is 25mm, and the height of the fixed block 3 is 10mm.
[0037] In this embodiment, a limiting strip is provided at the bottom of the inner box 1, spaced apart from the inclined slope 6, with a distance of 0.5mm between the limiting strip and the inclined slope 6. This design allows the limiting strip to restrain the bottom of the cell crawler 5 when the negative pressure suction between the cell crawler 5 and the inclined slope 6 is too weak to adsorb or when the cell crawler 5 accidentally detaches, preventing it from sliding and causing damage. Specifically, the distance between the limiting strip and the inclined slope 6 can be set according to requirements.
[0038] In the embodiment, the inclined slope surface 6 is provided with a strip-shaped groove extending along the length direction thereof to the end thereof. In this way, the negative pressure between the strip-shaped groove and the cell crawling sheet 5 can be avoided, the overall negative pressure suction force between the cell crawling sheet 5 and the inclined slope surface 6 of the fixing block 3 can be reduced, and thus the cell crawling sheet 5 can be more easily taken off, and the risk of violent clamping and crushing of the cell crawling sheet 5 caused by excessive suction force can be avoided. Specifically, the inclined slope surface 6 is provided with a plurality of grooves. In this way, when the cell crawling sheet 5 is placed on the inclined slope surface 6, the negative pressure suction force can be formed at the grooves, and the position stability of the cell crawling sheet 5 during cleaning can be improved.
[0039] In an alternative embodiment, the inclined slope surface 6 is provided with a limiting groove extending along the height direction thereof to the end thereof. The width of the limiting groove is 20 mm. In this way, the cell crawling sheet can be placed in the limiting groove, and the cell crawling sheet can be limited in this way, so as to avoid the cell crawling sheet from being tilted and damaged due to sliding caused by the influence of the cleaning liquid flow in a non-human case.
[0040] In an alternative embodiment, the side wall of the fixing block 3 is provided with a through hole. In this way, the overall negative pressure suction force between the cell crawling sheet 5 and the inclined slope surface 6 of the fixing block 3 can be reduced, and thus the cell crawling sheet 5 can be more easily taken off.
[0041] In the embodiment, the top of the fixing block 3 is provided with a mark. The mark can be a digital mark, which is convenient for managing the cell crawling sheet and avoiding confusion when recording experimental data.
[0042] In summary, the incubation box convenient for cleaning the cell crawling sheet can automatically fix the cell crawling sheet 5 on the inclined slope surface 6 of the fixing block 3 in an adsorbed manner after the cell crawling sheet 5 is soaked by the liquid, and the cell crawling sheet 5 can be avoided from being damaged due to tilting. Since one end of the fixing block 3 is spaced apart from the side wall of the box body 1, the cleaning liquid can be injected from the space between the fixing block 3 and the box body 1 when the cell crawling sheet 5 is cleaned, so as to avoid the direct impact of the cleaning liquid on the cell crawling sheet 5, and the cells on the cell crawling sheet 5 can be avoided from falling off. Since the height of the inclined slope surface 6 is less than the height of the cell crawling sheet 5, after the cell crawling sheet 5 is cleaned, the edge part not in contact with the inclined slope surface 6 of the fixing block 3 can be clamped by the tweezers, the cell crawling sheet 5 can be slowly moved out, the cell crawling sheet 5 can be gradually separated from the inclined slope surface 6 of the fixing block 3, and the cell crawling sheet 5 can be avoided from being crushed due to repeated use of the tweezers or improper force.
[0043] Obviously, the above embodiments are only examples for clearly illustrating the present application and are not intended to limit the present application. Based on the above description, other different forms of changes or variations can be made by those skilled in the art. Here, all the embodiments are not required to be enumerated. The changes or variations derived therefrom are still within the protection scope of the present application.
Claims
1. An incubation box for facilitating washing of cell-crawled slides, characterized in that, The utility model relates to a cell culture box, including box (1) and the fixed block (3) of setting in the box (1), the fixed block (3) one end is arranged at the side wall of the box (1) interval, the fixed block (3) one side has the inclined slope (6) of inclined arrangement, cell climbing piece (5) is suitable for leaning on the inclined slope (6) of setting, the height of the slope of the inclined slope (6) is less than cell climbing piece (5).
2. The incubation box for facilitating washing of cell-crawled slides according to claim 1, wherein, The inclined slope (6) is provided with a strip-shaped groove extending along the length direction thereof.
3. The incubation box for facilitating washing of cell-crawled slides according to claim 1, wherein, The box (1) is provided with a plurality of fixed blocks (3) fixed to the bottom of the box (1).
4. The incubation box for facilitating washing of cell-crawled slides according to claim 1, wherein, The box (1) is provided with a plurality of partitions (4) arranged at intervals, and the plurality of partitions (4) divide the box (1) into a plurality of cleaning tanks of the same size.
5. The incubation box for facilitating washing of cell climb sheets according to claim 4, wherein, The cleaning tank is provided with a plurality of fixed blocks (3), and the plurality of fixed blocks (3) are arranged side by side in the cleaning tank.
6. The incubation box for facilitating washing of cell climb sheets according to claim 4, wherein, One end of the fixed block (3) is arranged at an interval from the partition (4) or the side wall of the box (1), and the other end of the fixed block (3) is fixed to the side wall of the partition (4) or the side wall of the box (1).
7. The incubation box for facilitating washing of cell climb sheets according to claim 6, characterized in that, The interval between the two adjacent fixed blocks (3) in the same cleaning tank is 10-20 mm.
8. The incubation box for facilitating washing of cell-crawled slides according to claim 4, wherein, The interval between the other end of the fixed block (3) and the partition (4) or the side wall of the box (1) is 10-20 mm.
9. The incubation box for facilitating washing of cell climb sheets according to claim 1, wherein, The inclination of the inclined slope (6) of the fixed block (3) is 45-75 degrees.
10. The incubation box for facilitating washing of cell climb sheets according to claim 1, wherein, The height of the fixed block (3) is 8-14 mm.