Detachable elisa plate
By designing a detachable positioning frame structure for ELISA plates, the problems of poor stability and difficult cleaning of ELISA plates were solved, enabling multiple reuses and flexible assembly, reducing replacement costs and improving experimental efficiency.
Patent Information
- Application Number
- CN202422997710.8
- Authority / Receiving Office
- CN · China
- Patent Type
- Utility models(China)
- Current Assignee / Owner
- Filing Date
- 2024-12-05
- Publication Date
- 2025-12-23
- Estimated Expiration
- 2034-12-05
AI Technical Summary
Existing detachable ELISA plates have poor stability, are difficult to clean, and can only be used once, which cannot meet the need for multiple reuses.
A detachable enzyme-labeled plate was designed, which adopts a detachable positioning frame structure, including a circular positioning hole formed by a strip positioning plate and a semi-arc plate. The enzyme-labeled tube is connected by a sliding snap-fit mechanism with protrusions and through-slits, and then fixed with fastening bolts, so as to achieve stable connection and convenient disassembly of the enzyme-labeled tube.
This technology enables the repeated use of ELISA plates, provides good stability, facilitates cleaning, reduces replacement costs, and improves usage flexibility and experimental efficiency.
Smart Images
Figure CN223711612U_ABST
Abstract
Description
Technical Field
[0001] This utility model relates to the field of enzyme-linked immunosorbent assay (ELISA) plate technology, and in particular to a detachable ELISA plate. Background Technology
[0002] ELISA plates are commonly used in enzyme-linked immunosorbent assays (ELISA) and play an important role in immunological research. ELISA plates primarily serve as solid-phase carriers, holding antigens, antibodies, and other reactants to provide the basis for immunological reactions. Commonly available sizes include 48-well or 96-well plates, mainly used in conjunction with ELISA readers for detection. In the experiment, antigens, antibodies, and other biomolecules are adsorbed onto the surface of the ELISA plate through various mechanisms, then react with the sample being tested, and finally detected by the ELISA reader.
[0003] ELISA plates have wide applications in clinical medical diagnosis, immunology, and transgenic product identification. They are made of transparent polystyrene (GPPS) and undergo surface modification to achieve high transparency and sensitivity. Depending on experimental requirements, ELISA plates are available in various series, including high binding strength, medium binding strength, and amination, ensuring the reliability of experimental results. Currently, commonly used ELISA plates include both detachable and non-detachable structures. However, commonly used detachable ELISA plates suffer from poor stability, while non-detachable ELISA plates are difficult to clean and can only be used once. Therefore, this invention provides a detachable ELISA plate that can be reused multiple times. Utility Model Content
[0004] To address the problems of poor stability, difficulty in cleaning, and single-use limitation in existing technologies, this utility model discloses a detachable enzyme-linked immunosorbent assay (ELISA) plate.
[0005] The specific technical solution of this utility model is as follows:
[0006] This invention provides a detachable enzyme-linked immunosorbent assay (ELISA) plate, comprising a solid-phase plate and a plurality of ELISA tubes disposed on the solid-phase plate. The solid-phase plate has a storage slot, and a detachable positioning frame is provided within the storage slot. The plurality of ELISA tubes are detachably connected to the positioning frame.
[0007] The positioning frame includes multiple strip positioning plates, with both ends of the strip positioning plates snapped onto the side wall of the storage slot. Multiple longitudinal semi-arc plates are evenly arranged on the side of the strip positioning plates, and the semi-arc plates on adjacent strip positioning plates are joined together to form a circular positioning hole. The enzyme label tube is detachably snapped into the circular positioning hole.
[0008] Furthermore, the inner diameter of the circular positioning hole is the same as the outer diameter of the enzyme-labeled tube.
[0009] Furthermore, a through-slit is formed at the junction of the two semi-arc plates inside the circular positioning hole, and an annular groove is formed on the inner wall of the semi-arc plate. The annular groove communicates with the through-slit. A protrusion is provided on the outer wall of the enzyme labeling tube. The protrusion is adapted to the through-slit and the annular groove and can slide along the through-slit and the annular groove.
[0010] Furthermore, the strip positioning plate has a bayonet at both ends, which is engaged with the side wall of the storage slot. The end of the strip positioning plate is provided with a fastening bolt on the outer side wall of the bayonet. The side wall of the storage slot is evenly provided with a plurality of screw holes that are adapted to the fastening bolts. The plurality of strip positioning plates are fixed to the side wall of the storage slot by the fastening bolts.
[0011] Furthermore, a marking piece is provided at the connection between the top of the strip positioning plate and the semi-circular plate.
[0012] Furthermore, both the solid plate and the strip positioning plate are made of transparent material.
[0013] The beneficial effects of this utility model are as follows: The detachable enzyme-labeled plate provided by this utility model can detachably connect the solid phase plate, positioning frame and enzyme-labeled tube. When in use, it can be assembled as needed, and multi-well assembly can be realized. After assembly, the connection is not only stable, but also easy to clean and can be reused multiple times. The detachable assembly method can replace the component in time when one component is damaged, without replacing the whole component, which effectively saves costs. Attached Figure Description
[0014] Figure 1 This is a structural diagram of the detachable enzyme-labeled plate of this utility model;
[0015] Figure 2 This is an enlarged view of the semi-circular plate assembly of the detachable enzyme-labeled plate in this utility model;
[0016] Figure 3 for Figure 1 Enlarged view of A in the middle;
[0017] Figure 4 This is an enlarged view of the enzyme labeling tube structure of the detachable enzyme labeling plate in this utility model;
[0018] Figure 5 This is an assembly diagram of the semi-arc plate and enzyme-labeled tube in this utility model.
[0019] In the diagram: 10, solid plate; 20, enzyme-labeled tube; 31, strip positioning plate; 11, storage slot; 111, screw hole; 21, protrusion; 311, semi-circular plate; 312, through slot; 313, annular groove; 314, bayonet; 315, fastening bolt. Detailed Implementation
[0020] The present invention will be further described in detail below with reference to the following embodiments.
[0021] like Figure 1 As shown, this utility model provides a detachable ELISA plate, specifically including a solid plate 10 and multiple ELISA tubes 20 disposed on the solid plate 10. The solid plate 10 is similar to a fixed base. The ELISA tubes 20 are used to add test samples or reagents. A storage slot 11 is opened on the solid plate 10 for placing the ELISA tubes 20. When not performing an experiment, the ELISA tubes 20 can be stored in the storage slot 11. A detachable positioning frame is provided in the storage slot 11 for supporting and fixing the ELISA tubes 20. Multiple ELISA tubes 20 are detachably connected to the positioning frame, which facilitates cleaning of the ELISA tubes 20 or replacement of the positioning frame. When the ELISA tubes 20 or the positioning frame are damaged, individual parts can be replaced without replacing the entire plate.
[0022] Specifically, the positioning frame includes multiple strip positioning plates 31, with both ends of the strip positioning plates 31 snapped onto the side wall of the storage slot 11. The strip positioning plates 31 can be assembled as needed, not limited to 48 holes or 96 holes, and can be assembled according to requirements, making it more flexible to use.
[0023] like Figure 2-5 As shown, to secure the enzyme-labeled tube 20 and ensure its stability, multiple longitudinal semi-circular plates 311 are evenly arranged on the side of the strip positioning plate 31. The semi-circular plates 311 on adjacent strip positioning plates 31 are joined to form circular positioning holes, and the enzyme-labeled tube 20 is detachably snapped into the circular positioning holes. During use, snapping the enzyme-labeled tube 20 into the circular positioning holes ensures that the enzyme-labeled tube 20 will not tilt, making assembly and use more convenient and quick.
[0024] like Figure 2-5 As shown, in some embodiments, the inner diameter of the circular positioning hole is the same as the outer diameter of the enzyme labeling tube 20, so that the enzyme labeling tube 20 can be directly placed into the circular positioning hole.
[0025] In order to stably place the enzyme labeling tube 20 in the circular positioning hole, in some embodiments, it is further specified that a through-slit 312 is formed at the joint of the two semi-arc plates 311 inside the circular positioning hole, and an annular groove 313 is formed on the inner wall of the semi-arc plates 311. The annular groove 313 communicates with the through-slit 312. The outer wall of the enzyme labeling tube 20 is provided with a protrusion 21, which is adapted to the through-slit 312 and the annular groove 313 and can slide along the through-slit 312 and the annular groove 313. In use, the protrusion 21 on the outer wall of the enzyme labeling tube 20 slides vertically into the circular positioning hole through the through-slit 312. When the protrusion 21 reaches the ring groove 313, rotate the enzyme labeling tube 20 to make the protrusion 21 slide into the ring groove 313. At this time, the enzyme labeling tube 20 can be stably locked into the circular positioning hole and can be stably fixed. When not in use or during cleaning, it can be rotated in the opposite direction until the protrusion 21 slides into the through-slit 312, and then the enzyme labeling tube 20 can be removed from the circular positioning hole. Disassembly is extremely convenient and more stable.
[0026] To ensure assembly stability, some embodiments further specify that the strip positioning plate 31 has slots 314 at both ends, which engage with the side wall of the storage slot 11. A fastening bolt 315 is located on the outer side wall of the slot 314 at the end of the strip positioning plate 31. Several screw holes 111, adapted to the fastening bolts 315, are evenly distributed on the side wall of the storage slot 11. Multiple strip positioning plates 31 are fixed to the side wall of the storage slot 11 by the fastening bolts 315. By engaging the two ends of the strip positioning plate 31 with the side wall of the storage slot 11 and fixing it with the fastening bolts 315, greater stability is achieved. Furthermore, the storage slot 11 has a large space, allowing experimenters to select the fixing position of the strip positioning plate 31 as needed, facilitating experimental operations.
[0027] In some embodiments, a marking sheet is further specified at the connection between the top of the strip positioning plate 31 and the semi-circular plate 311. The marking sheet can be used for recording or marking, which is convenient for experimental personnel to conduct experiments, so as to prevent forgetting to add solution or adding reagents repeatedly, which is more in line with the actual needs of experimental personnel.
[0028] In some embodiments, it is further specified that both the solid plate 10 and the strip positioning plate 31 are made of transparent material. The transparent material can be polypropylene. The transparent solid plate 10 and the strip positioning plate 31 have good light transmittance, which can transmit the fluorescence or radioactive signal of the detection result to the reader.
[0029] This utility model is not limited to the above-described preferred embodiments. Anyone can derive other forms of products under the guidance of this utility model. However, regardless of any changes made in their shape or structure, any technical solution that is the same as or similar to this application falls within the protection scope of this utility model.
Claims
1. A detachable enzyme-linked immunosorbent assay (ELISA) plate, characterized in that, The device includes a solid-phase plate (10) and a plurality of enzyme-labeled tubes (20) disposed on the solid-phase plate (10). A material storage slot (11) is formed on the solid-phase plate (10), and a detachable positioning frame is provided within the material storage slot (11). The plurality of enzyme-labeled tubes (20) are detachably connected to the positioning frame. The positioning frame includes multiple strip positioning plates (31), with both ends of the strip positioning plates (31) snapped onto the side wall of the storage slot (11). Multiple longitudinal semi-arc plates (311) are evenly arranged on the side of the strip positioning plates (31). The semi-arc plates (311) on adjacent strip positioning plates (31) are joined together to form a circular positioning hole. The enzyme label tube (20) is detachably snapped into the circular positioning hole.
2. The detachable ELISA plate as described in claim 1, characterized in that, The inner diameter of the circular positioning hole is the same as the outer diameter of the enzyme labeling tube (20).
3. The detachable enzyme-linked immunosorbent assay (ELISA) plate as described in claim 2, characterized in that, The circular positioning hole forms a through-slit (312) at the junction of the two semi-arc plates (311). An annular groove (313) is formed on the inner wall of the semi-arc plate (311), and the annular groove (313) communicates with the through-slit (312). The outer wall of the enzyme labeling tube (20) is provided with a protrusion (21), which is adapted to the through-slit (312) and the annular groove (313) and can slide along the through-slit (312) and the annular groove (313).
4. The detachable enzyme-labeled plate as described in claim 3, characterized in that, The strip positioning plate (31) has a bayonet (314) at both ends, which is engaged with the side wall of the storage slot (11). The end of the strip positioning plate (31) is provided with a fastening bolt (315) on the outer side wall of the bayonet (314). The side wall of the storage slot (11) is evenly provided with a plurality of screw holes (111) that are adapted to the fastening bolt (315). The multiple strip positioning plates (31) are fixed to the side wall of the storage slot (11) by the fastening bolt (315).
5. The detachable enzyme-labeled plate as described in claim 4, characterized in that, A marking piece is provided at the connection between the top of the strip positioning plate (31) and the semi-arc plate (311).
6. The detachable enzyme-linked immunosorbent assay (ELISA) plate as described in claim 5, characterized in that, Both the solid plate (10) and the strip positioning plate (31) are made of transparent material.