Surgical instrument for mouse ovary orthotopic transplantation

Abstract

The utility model discloses a surgical instrument for mouse ovary orthotopic transplantation, and belongs to the technical field of mouse ovary transplantation, the surgical instrument comprises a sleeve, a supporting rod is mounted in the sleeve, a clamping piece and an expansion piece are mounted at the bottom of the supporting rod, the clamping piece comprises two clamping rods, the bottom ends of the two clamping rods can abut against each other, and the expansion piece is mounted at the bottom of the supporting rod. The expansion piece comprises two expansion rods, and the bottom ends of the two expansion rods can expand towards the outer side. The method has the advantages of being high in transplanting speed and high in accuracy.

Description

TECHNICAL FIELD

[0001] The present application relates to the technical field of mouse ovary transplantation, and particularly to a surgical instrument for in-situ transplantation of mouse ovary. BACKGROUND

[0002] Mouse ovary transplantation technology refers to transplanting ovary tissue of a donor mouse into a recipient mouse through surgery, and adopting in-situ transplantation or ectopic transplantation to make the ovary tissue undergo vascular reconstruction and restore normal physiological functions in the recipient mouse.

[0003] The ovary tissue itself contains abundant angiogenic factors, which is conducive to the formation of new blood vessels in the body and has strong natural plasticity. In the process of ovary transplantation, the accuracy of the transplantation position and the transplantation speed are crucial, because ovary transplantation is an invasive operation, and improper position can easily cause adhesion between the ovary and the surrounding tissue; too long time of ovary transplantation or ex vivo can cause ischemia of the ovary tissue and follicle atresia, thereby seriously damaging the ovary tissue and losing its potential physiological functions after transplantation.

[0004] In the in-situ transplantation of mouse ovary, the ovary tissue structure needs to be exposed, the ovary capsule is opened, the ovary of the recipient mouse is removed, the ovary of the donor mouse is transplanted into the capsule, and finally the entire ovary tissue is sent back into the mouse for surgical suture.

[0005] There are some difficulties in the transplantation process. The ovary capsule has a certain elasticity and is very thin. When the ovary is removed, the size of the opening of the ovary capsule is very important. If the opening is too small, the ovary tissue cannot be fully exposed and it is difficult to remove; if the opening is too large, the capsule cannot wrap and fix the transplanted ovary tissue, and the ovary is easy to slip off, which may run to other positions in the mouse body during the process of being sent back to the abdominal cavity, thus it is difficult to ensure the accuracy of the transplantation position and the transplantation speed, thereby causing tissue adhesion or failure to restore the physiological functions of the transplanted ovary. CONTENT OF THE UTILITY MODEL

[0006] In order to ensure the accuracy of the transplantation position and improve the transplantation speed, and to reduce the occurrence of tissue adhesion and improve the recovery effect of the potential physiological functions of the ovary after transplantation as much as possible, the present application provides a surgical instrument for in-situ transplantation of mouse ovary.

[0007] The surgical instrument for in-situ transplantation of mouse ovary provided by the present application adopts the following technical solution:

[0008] The surgical instrument for in-situ transplantation of mouse ovary comprises a sleeve, a supporting rod is installed in the sleeve, a clamping piece and a dilating piece are installed at the bottom of the supporting rod, the clamping piece comprises two clamping rods, the bottom ends of the two clamping rods can abut against each other, the dilating piece comprises two dilating rods, and the bottom ends of the two dilating rods can be dilated outward.

[0009] By adopting the technical scheme, the two clamping rods are used to clamp the ovary of the donor mouse and transport it to the ovary envelope of the recipient mouse, the ovary is clamped and gently forced, the end of the expansion rod is pressed against the ovary envelope, the ovary envelope is expanded horizontally outward, the clamping rod is released, the ovary is accurately placed in the ovary envelope, the ovary envelope is fully wrapped, the ovary tissue is not easy to slip off, the whole operation is very fast, the transplantation speed is fast and the accuracy is high.

[0010] Preferably, the clamping rod comprises a connecting part fixed to the bottom end of the supporting rod and a clamping part fixed to the bottom end of the connecting part, the bottom ends of the two connecting parts are inclined towards each other away from each other, the bottom ends of the two clamping parts are inclined towards each other, and the bottom end of the clamping part is a sharp end.

[0011] By adopting the technical scheme, the inclination of the clamping rod makes the clamping more stable, and the sharp end design helps to accurately clamp the ovary and reduce damage.

[0012] Preferably, the top end of the expansion rod is fixed with a connecting piece, the top end of the connecting piece is fixedly connected with the bottom end of the supporting rod, the connecting piece is a rigid elastic piece, the bottom end of the expansion rod is provided with a supporting part, and the supporting part is located on the side away from each other of the two expansion rods.

[0013] By adopting the technical scheme, the connecting piece provides stable connection for the expansion rod, ensuring the stability and durability of the expansion rod during operation; the design of the supporting part helps to disperse the force and improve the strength and reliability of the expansion rod.

[0014] Preferably, the top end of the supporting rod is fixed with a mounting piece, and the bottom surface of the mounting piece can be attached to the top surface of the sleeve.

[0015] By adopting the technical scheme, the mounting piece is attached to the top surface of the sleeve to ensure the close connection between the supporting rod and the sleeve, and improve the stability of the whole instrument.

[0016] Preferably, the bottom surface of the connecting piece is provided with a moving slot, a moving rod is vertically slidably installed in the moving slot, a rotating rod is rotatably installed at the bottom end of the moving rod, two hinged rods are sleeved on the outer periphery of the rotating rod, one end of the hinged rod away from the rotating rod is hinged to the middle part of the expansion rod, and the included angle of the two hinged rods is less than 180°.

[0017] By adopting the technical scheme, when the two expansion rods expand the ovary envelope towards each other away from each other, the moving rod and the hinged rod can limit the opening angle of the expansion rod, thereby reducing the possibility of over-expanding the ovary envelope.

[0018] Preferably, the side of the moving rod is fixed with a sliding block, the inner wall of the moving groove is provided with a sliding groove, and the sliding block is in sliding cooperation with the connecting plate through the sliding groove.

[0019] By adopting the above technical scheme, the sliding block and the sliding groove limit the position of the moving rod, thereby limiting the expansion angle of the expansion rod.

[0020] Preferably, the outer periphery of the two expansion rods is provided with a limiting frame, the limiting frame is located at the top of the expansion rod, and the outer periphery of the expansion rod is provided with two fixed rings.

[0021] By adopting the above technical scheme, the limiting frame and the fixed ring limit the movement range of the expansion rod, prevent over-expansion, and protect the ovarian capsule from damage.

[0022] Preferably, two limiting blocks are slidingly installed in the limiting frame along the length direction of the limiting frame, threaded holes are formed in both ends of the limiting frame, bolts are penetratingly arranged in the threaded holes, the bolts are in threaded connection with the limiting frame through the threaded holes, and the end of the bolt is in rotary connection with the limiting block.

[0023] By adopting the above technical scheme, the rotary bolt can adjust the distance between the two limiting blocks, thereby adjusting the maximum expansion limit of the two expansion rods, which is simple in operation and improves the applicability of the expansion rod.

[0024] In summary, the present application has at least one of the following beneficial technical effects:

[0025] 1. The donor mouse ovary is clamped and transported to the recipient mouse ovarian capsule by two clamping rods, the ovary is clamped and gently forced, the end of the expansion rod abuts against the ovarian capsule, and the ovarian capsule is expanded horizontally outward, the clamping rod is loosened, the ovary is accurately placed in the ovarian capsule, the capsule is fully wrapped, the ovarian tissue is not easy to slip off, the whole operation is very rapid, the transplantation speed is faster and the accuracy is higher;

[0026] 2. When the two expansion rods expand the ovarian capsule in the direction away from each other, the movement rod and the hinged rod can limit the opening angle of the expansion rod, thereby reducing the possibility of over-expansion of the ovarian capsule;

[0027] 3. The limiting frame and the fixed ring limit the movement range of the expansion rod, prevent over-expansion, protect the ovarian capsule from damage, the rotary bolt can adjust the distance between the two limiting blocks, thereby adjusting the maximum expansion limit of the two expansion rods, which is simple in operation and improves the applicability of the expansion rod. BRIEF DESCRIPTION OF DRAWINGS

[0028] Figure 1is a schematic diagram of the overall structure of Example 1.

[0029] Figure 2 is a schematic diagram of the structure of the support rod in Example 1.

[0030] Figure 3 is a schematic diagram of the overall structure of Example 2.

[0031] Figure 4 is a sectional view of the connecting piece in Example 2.

[0032] Figure 5 is Figure 4 is an enlarged schematic diagram at A in FIG.

[0033] Figure 6 is a schematic diagram of the overall structure of Example 1.

[0034] Figure 7 is Figure 6 is an enlarged schematic diagram at B in FIG.

[0035] Reference signs: 1, sleeve; 11, support rod; 12, mounting piece; 2, clamping piece; 21, clamping rod; 22, connecting portion; 23, clamping portion; 3, expansion piece; 31, expansion rod; 32, connecting piece; 33, support portion; 4, moving rod; 41, moving groove; 42, sliding block; 43, sliding groove; 44, rotating rod; 45, hinged rod; 5, limiting frame; 51, fixed ring; 52, limiting block; 53, threaded through hole; 54, bolt. DETAILED DESCRIPTION

[0036] The following will be described in detail below with reference to the accompanying drawings. Figures 1-7 The application will be described in further detail.

[0037] The application discloses a surgical instrument for in-situ transplantation of mouse ovaries.

[0038] Example 1

[0039] With reference to Figure 1 and Figure 2 The surgical instrument for in-situ transplantation of mouse ovaries comprises a sleeve 1, and a support rod 11 is mounted in the sleeve 1. The top end of the support rod 11 is fixed with a mounting piece 12, and the bottom surface of the mounting piece 12 can be attached to the top surface of the sleeve 1.

[0040] With reference to Figure 1 and Figure 2The bottom of the support rod 11 is provided with the clamping piece 2 and the expansion piece 3. The clamping piece 2 comprises two clamping rods 21, and the bottom ends of the two clamping rods 21 can abut each other. The clamping rod 21 comprises a connecting portion 22 fixed to the bottom end of the support rod 11 and a clamping portion 23 fixed to the bottom end of the connecting portion 22, and the bottom ends of the two connecting portions 22 are inclined towards each other away from each other. The bottom ends of the two clamping portions 23 are inclined towards each other, and the bottom end of the clamping portion 23 is a pointed end.

[0041] With reference to Figure 1 and Figure 2 , the expansion piece 3 comprises two expansion rods 31, and the bottom ends of the two expansion rods 31 can be expanded outward. The top end of the expansion rod 31 is fixedly connected with the connecting sheet 32, and the top end of the connecting sheet 32 is fixedly connected with the bottom end of the support rod 11. The connecting sheet 32 is a rigid elastic sheet. The bottom end of the expansion rod 31 is provided with a support portion 33, and the support portion 33 is located on the side away from each other of the two expansion rods 31.

[0042] The implementation principle of the embodiment 1 is as follows: first, the recipient mouse is anesthetized, and after the mouse has no pain reaction, the surgical area is disinfected, and the skin and muscle are opened in turn to expose the ovary structure. According to the size of the ovary, a small incision is made on the ovary capsule with a Venus scissors, and the capsule is gently pried open to fully expose the ovary tissue. Then, the ovary base is clamped with the clamping rod 21, and the ovary tissue is quickly removed. Hemostasis is performed immediately.

[0043] Meanwhile, the donor mouse is euthanized, and its ovary is quickly removed. The donor mouse ovary is clamped with the clamping rod 21, and is transported to the ovary capsule of the recipient mouse. The ovary is clamped and gently forced, the expansion rod 31 opens the ovary capsule, and the support portion 33 at the bottom end of the expansion rod 31 abuts against the ovary capsule to horizontally expand the ovary capsule outward. After the clamping rod 21 is released, the ovary can be accurately placed in the ovary capsule. The capsule is fully wrapped, the ovary tissue is not easy to slip off, the whole operation is very fast, the transplantation speed is fast and the accuracy is high.

[0044] Embodiment 2

[0045] With reference to Figure 3 , Figure 4 and Figure 5 , the difference between this embodiment and the embodiment 1 is that the bottom surface of the connecting sheet 32 is provided with a moving groove 41, and the connecting sheet 32 is vertically slidably installed with a moving rod 4 through the moving groove 41. The side surface of the moving rod 4 is fixedly connected with a sliding block 42, the inner wall of the moving groove 41 is provided with a sliding groove 43, and the sliding block 42 is vertically slidably connected with the connecting sheet 32 through the sliding groove 43. The bottom end of the moving rod 4 is rotatably connected with a rotating rod 44, and the outer periphery of the rotating rod 44 is sleeved with two hinged rods 45. The end of the hinged rod 45 away from the rotating rod 44 is hingedly connected with the middle portion of the expansion rod 31, and the included angle between the two hinged rods 45 is less than 180°.

[0046] The implementation principle of the embodiment 2 is that when the two expansion rods 31 expand the ovarian capsule in the direction away from each other, the movable rod 4 and the hinged rod 45 can limit the opening angle of the expansion rod 31, thereby reducing the possibility of overexpansion of the ovarian capsule.

[0047] Embodiment 3

[0048] With reference to Figure 6 and Figure 7 The difference between the embodiment and the embodiment 1 is that the outer periphery of the two expansion rods 31 is sleeved with a limiting frame 5, and the limiting frame 5 is located at the top of the expansion rod 31. The outer periphery of the expansion rod 31 is sleeved with two fixed rings 51, and the limiting frame 5 is located between the two fixed rings 51. Two limiting blocks 52 are slidably installed in the limiting frame 5 along the length direction of the limiting frame 5, threaded through holes 53 are formed in both ends of the limiting frame 5, and bolts 54 are threaded through the threaded through holes 53.

[0049] The implementation principle of the embodiment 3 is that rotating the bolt 54 can adjust the distance between the two limiting blocks 52, thereby adjusting the maximum expansion limit of the two expansion rods 31, which is simple to operate and improves the applicability of the expansion rod 31.

[0050] The above are preferred embodiments of the present application, and are not intended to limit the protection scope of the present application. Therefore, any equivalent changes made on the basis of the structure, shape, and principle of the present application shall be covered within the protection scope of the present application.

Claims

1. A surgical instrument for mouse ovarian orthotopic transplantation, characterized in that: The utility model provides a kind of sleeve (1), support rod (11) is installed in the sleeve (1), the bottom of support rod (11) is equipped with clamping piece (2) and expansion piece (3), clamping piece (2) includes two clamping rods (21), the bottom end of two clamping rods (21) can be mutually abutted, expansion piece (3) includes two expansion rods (31), the bottom end of two expansion rods (31) can expand to outside side; The top end of the expansion rod (31) is fixed with a connecting sheet (32), the top end of the connecting sheet (32) is fixedly connected with the bottom end of the support rod (11), the connecting sheet (32) is a rigid elastic sheet, the bottom end of the expansion rod (31) is provided with a support portion (33), and the support portion (33) is located on the side where the two expansion rods (31) are away from each other. A moving groove (41) is formed on the bottom surface of the connecting sheet (32), and a moving rod (4) is vertically slidably installed in the moving groove (41). The bottom end of the moving rod (4) is rotatably installed with a rotating rod (44), and the outer periphery of the rotating rod (44) is sleeved with two hinged rods (45). One end of the hinged rod (45) away from the rotating rod (44) is hinged to the middle part of the expansion rod (31), and the included angle between the two hinged rods (45) is less than 180°.

2. The surgical instrument for orthotopic transplantation of mouse ovary according to claim 1, characterized in that: The clamping rod (21) includes a connecting portion (22) fixed to the bottom end of the support rod (11) and a clamping portion (23) fixed to the bottom end of the connecting portion (22). The bottom ends of the two connecting portions (22) are inclined towards each other away from each other. The bottom ends of the two clamping portions (23) are inclined towards each other. The bottom end of the clamping portion (23) is a sharp end.

3. The surgical instrument for orthotopic transplantation of mouse ovary according to claim 1, characterized in that: The top end of the support rod (11) is fixed with a mounting sheet (12), and the bottom surface of the mounting sheet (12) can be fitted with the top surface of the sleeve (1).

4. The surgical instrument for orthotopic transplantation of mouse ovary according to claim 1, characterized in that: A sliding block (42) is fixed to the side surface of the moving rod (4), and a sliding groove (43) is formed on the inner wall of the moving groove (41). The sliding block (42) is vertically slidably matched with the connecting sheet (32) through the sliding groove (43).

5. The surgical instrument for orthotopic transplantation of mouse ovary according to claim 1, characterized in that: The outer periphery of the two expansion rods (31) is provided with a limiting frame (5), and the limiting frame (5) is located at the top of the expansion rod (31). The outer periphery of the expansion rod (31) is sleeved with two fixed rings (51), and the limiting frame (5) is located between the two fixed rings (51).

6. The surgical instrument for orthotopic transplantation of mouse ovary according to claim 5, characterized in that: Two limiting blocks (52) are slidably installed in the limiting frame (5) along the length direction of the limiting frame (5). Threaded holes (53) are formed at both ends of the limiting frame (5), and bolts (54) are penetratingly arranged in the threaded holes (53). The bolts (54) are threadedly connected with the limiting frame (5) through the threaded holes (53), and the end portions of the bolts (54) are rotatably connected with the limiting blocks (52).

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