Nanopore processing fluid pool carrying platform

Abstract

The utility model discloses a nanopore processing fluid pool platform deck, which relates to the technical field of nanopore processing, and comprises a fluid pool platform deck, a fluid pool assembly and a fluid pool clamping assembly, the fluid pool assembly is composed of a left fluid pool and a right fluid pool which are in butt joint with each other, the fluid pool carrying table is composed of a fluid pool carrying table fixing seat, a crown spring locking seat, a first crown spring female needle and a first crown spring locking gasket, the fluid pool carrying table fixing seat is of a U-shaped structure, and the first crown spring female needle is installed on the left side of the fluid pool carrying table fixing seat through the crown spring locking seat and the first crown spring locking gasket. According to the utility model, a detachable connecting structure is adopted at the joint of the two fluid pools, a gasket, a chip and a gasket which are matched can be selected for butt joint according to a chip to be processed, the fluid pools can be conveniently clamped and fixed by using the carrying table, the chip is convenient to clamp the fluid pools and the fluid pools are convenient to take, so that the working efficiency and the experience feeling of a user are further improved, and the production cost is reduced. And the punching success efficiency is improved.

Description

Technical Field

[0001] This utility model belongs to the field of nanopore processing technology, specifically relating to a fluid pool platform for nanopore processing. Background Technology

[0002] Nanopore sensing technology, as a third-generation sequencing technology, has become a widely researched and applied biosensing technology due to its advantages such as high throughput, long read length, and low cost. Simultaneously, the fabrication of solid-state nanopore sensors with small pore sizes and strong robustness is essential. Currently, methods for fabricating solid-state nanopores mainly include ion beam etching, electron beam sputtering etching, electrochemical etching, and the emerging dielectric breakdown method. Because dielectric breakdown drilling does not require a beam line of sight to create nanopores, it can fabricate planar nanopores within existing nanostructures. Furthermore, dielectric breakdown drilling is cost-effective and can be easily implemented with minimal training using inexpensive hardware, making it a popular drilling technique in many laboratories.

[0003] However, the current operation and use of fluidic cells for nanopore fabrication is still in the laboratory stage. Typically, the fluidic cell is clamped and fastened with screws, requiring manual tightening each time. This results in inconsistent skill levels among operators and varying clamping force. Excessive force risks crushing the chip being processed, while insufficient force results in insufficient sealing and leakage, making it impossible to proceed to the next step. This current method of use affects work efficiency and also presents operational problems such as cumbersome procedures and difficult clamping. In addition, the connection between the fluidic cell and the external power source is also achieved by clamping with tweezers, which hinders the commercialization of the product. Utility Model Content

[0004] To address the problems existing in the prior art, this utility model provides a nanopore processing fluid pool platform, which improves the original laboratory setup that can only meet the needs of testing and experimental verification, and upgrades it to a testing and experimental environment setup for transforming products into commodities. The operation process is simple, easy to use, and easy to process and mass-produce, taking a big step towards the commercialization of products.

[0005] The solution adopted by this utility model to solve its technical problem is: a fluid pool stage for nanopore processing, including a fluid pool stage, a fluid pool assembly, and a fluid pool clamping assembly. The fluid pool assembly consists of two interlocking left and right fluid pools. The fluid pool stage consists of a fluid pool stage fixing seat, a crown spring locking seat, a crown spring female pin, and a crown spring locking washer. The fluid pool stage fixing seat has a U-shaped structure. A crown spring female pin is installed on the left side of the fluid pool stage fixing seat through the crown spring locking seat and the crown spring locking washer. The fluid pool clamping assembly includes a rotary button fixing seat fixed on the upper right side of the fluid pool stage fixing seat. A movable locking tongue is axially slidably fitted between the rotary button fixing seat and the fluid pool stage fixing seat. A crown spring female pin is installed inside the movable locking tongue through the crown spring locking washer, and a buffer rubber pad is installed on the front side of the crown spring locking washer.

[0006] Furthermore, solution channels are provided in the left and right fluid pools, and electrolyte holes communicating with the solution channels are provided at the top of the two fluid pools. Nylon pillars are installed through the outer sides of the two fluid pools, and sealing gaskets are provided at the connection between the inner end of the nylon pillar and the solution channel. Conductive silver wires are inserted into the nylon pillars, and the front end of the conductive silver wires is inserted into the solution channel. Sealing gaskets are provided at the joint on the inner side of the two fluid pools. The chip to be processed can be detachably installed in the fluid pool assembly.

[0007] Furthermore, a guide shaft fixing seat is fixed to the rear right side of the fluid pool platform fixing seat. Multiple locking tongue guide shafts are axially arranged on the guide shaft fixing seat. The movable locking tongue is guided by the locking tongue guide shafts and then directionally slidably fitted onto the right side of the fluid pool platform fixing seat.

[0008] Furthermore, the upper surface of the movable latch is provided with multiple slots along the moving direction. The slots are used to insert anti-push blocks to prevent the push blocks from engaging with the slots to position the movable latch.

[0009] Furthermore, a micro switch sensing component is installed on the fluid pool platform. The micro switch sensing component consists of a micro switch and a micro switch bracket. The micro switch bracket is fixed to one side of the fluid pool platform, and the micro switch is mounted on the micro switch bracket.

[0010] Furthermore, the fluid pool assembly is provided with an overflow hole, which is connected to the solution channel.

[0011] Compared with the prior art, the beneficial effects of this utility model are:

[0012] The connection between the two fluid pools in this invention adopts a detachable connection structure. Depending on the chip to be processed, a suitable gasket + chip + gasket sealing component and docking can be selected. The fluid pool can be easily clamped and fixed using a platform, making it convenient to clamp and remove the chip from the fluid pool, thereby improving work efficiency and user experience. The golden combination of sealing component + chip + sealing component solves the risk of chip leakage leading to solid nanopore processing failure and chip crushing leading to expensive chip waste during the clamping process of gasket chip and gasket, thereby improving the drilling success rate.

[0013] The fluid pool of this utility model has an electrolyte hole and an overflow hole. Through the inlet and outlet flow channels, the electrolyte can release air through the overflow hole during the process of flowing into the chip, so as to avoid the generation of bubbles that interfere with the nanopore processing of the chip.

[0014] The conductive silver wire of this invention can be connected to an external power source using a crown spring or spring pin, which improves the adaptability of experimental environment setup and allows solid nanopores to be upgraded from laboratory applications to commercial products, thus truly entering the market. Attached Figure Description

[0015] Figure 1 This is a schematic diagram of the fluid pool platform assembly of this utility model;

[0016] Figure 2 This is a schematic diagram of the explosion of the fluid pool platform of this utility model;

[0017] Figure 3 This is a schematic diagram of the fluid stage assembly of this utility model;

[0018] Figure 4 This is a schematic diagram of the fluid platform explosion of this utility model.

[0019] In the diagram: 1. Fluid pool platform; 101. Fluid pool platform fixing seat; 102. Crown spring locking seat; 103. Crown spring female pin one; 104. Crown spring locking gasket one; 2. Fluid pool assembly; 21. Left fluid pool; 22. Right fluid pool; 201. Conductive silver wire; 202. Nylon column; 203. Sealing gasket; 204. Electrolyte hole; 205. Sealing gasket; 206. Overflow hole; 3. Fluid pool clamping assembly; 301. Rotary button; 302. Rotary button fixing seat; 303. Anti-push block; 304. Locking tongue guide shaft; 305. Movable locking tongue; 306. Crown spring female pin two; 307. Crown spring locking gasket two; 308. Buffer rubber pad; 309. Guide shaft fixing seat; 4. Micro switch sensing assembly; 401. Micro switch; 402. Micro switch bracket; 5. Chip to be processed. Detailed Implementation

[0020] The present invention will be further described below with reference to the accompanying drawings and embodiments.

[0021] Please see Figure 1-4 This utility model provides a technical solution for a fluid pool platform for nanopore processing: Example

[0022] according to Figure 1 and Figure 3 As shown, the nanopore processing fluid pool stage mainly includes a fluid pool stage 1, a fluid pool assembly 2, and a fluid pool clamping assembly 3. The fluid pool assembly 2 consists of two interlocking fluid pools, a left fluid pool 21 and a right fluid pool 22. The specific structures of the two fluid pools are as follows... Figure 4 As shown, the device mainly includes a conductive silver wire 201, a nylon pillar 202, a sealing gasket 203, an electrolyte hole 204, an overflow hole 206, and a sealing gasket 205. Solution channels are provided in two fluid pools. An electrolyte hole 204 communicating with the solution channels is opened at the top of each fluid pool. A nylon pillar 202 is fitted through the outer sides of both fluid pools. A sealing gasket 203 is detachably installed at the connection between the inner end of the nylon pillar 202 and the solution channel. The nylon pillar 202 is used to insert the conductive silver wire 201, and the front end of the conductive silver wire 201 can be inserted into the solution channel. A sealing gasket 205 is provided at the inner joint of the two fluid pools. The chip to be processed 5 is detachably installed at the joint of the two fluid pools. When the two liquid pools are joined together, electrolyte is introduced into the two liquid pools and electrodes are connected, forming a dielectric within the solution channel, thereby performing nanopore processing on the chip to be processed 5 held in the middle.

[0023] The main function of the fluid pool assembly is to create nanopores for the chip to be processed. It is a core component of nanopore processing. The golden combination of gasket + chip + gasket is very important. If it is not suitable, it will lead to leakage at the chip connection or chip crushing, which will seriously affect the success rate of drilling and work efficiency. Therefore, the connection between the two fluid pools adopts a detachable connection structure. The appropriate gasket + chip + gasket sealing component can be selected according to the chip to be processed.

[0024] The electrolyte is primarily an alkaline liquid, and the sealing gasket 203 can be a perfluoroether gasket. Because air bubbles are easily generated during electrolyte dripping, preventing the liquid from properly wetting the chip and resulting in poor conductivity and unsuccessful drilling, a custom sealing gasket 205 is used at the interface of the fluid pool. An overflow hole 206 is added to the fluid pool, which is connected to the solution channel to solve the above problems and improve drilling efficiency and success rate. Specifically, the electrolyte flow channel into the chip is horizontal. The overflow hole allows air to be released during the electrolyte flow through the inlet and outlet channels, preventing air bubbles from interfering with the nanopore processing of the chip.

[0025] like Figure 2 As shown, the fluid pool platform 1 mainly consists of a fluid pool platform fixing seat 101, a crown spring locking seat 102, a crown spring female pin 103, and a crown spring locking washer 104. The fluid pool platform fixing seat 101 has a U-shaped structure. The bottom platform of the fluid pool platform fixing seat 101 is used to place the fluid pool assembly 2. The crown spring locking seat 102 and the crown spring locking washer 104 are fixedly mounted on the left side of the fluid pool platform fixing seat 101. The crown spring female pin 103 is mounted on the left side of the fluid pool platform fixing seat 101 through the crown spring locking washer 104. The outer end of the crown spring female pin 103 is stably installed on the left side of the fluid pool platform fixing seat 101 through the crown spring locking seat 102. The main function of this part is to serve as a support platform for the fluid pool. When the fluid pool is placed in, the fluid pool support platform fixing seat 101 positions the fluid pool. The crown spring female pin 103 connects with the conductive silver wire 201 of the left fluid pool 21, which enables the left fluid pool 21 to be connected to the external power supply.

[0026] The fluid pool clamping assembly 3 consists of a rotary button 301, a rotary button mounting base 302, an anti-push block 303, a locking tongue guide shaft 304, a movable locking tongue 305, a crown spring pin 306, a crown spring locking washer 307, a buffer rubber pad 308, and a guide shaft mounting base 309. The rotary button mounting base 302 is fixedly installed on the upper right side of the fluid pool platform mounting base 101. A movable locking tongue 305 is axially slidably fitted between the rotary button mounting base 302 and the fluid pool platform mounting base 101. A guide shaft mounting base 309 is fixedly installed on the rear right side of the fluid pool platform mounting base 101. Multiple locking tongue guide shafts 304 are axially arranged on the upper, lower, left, and right sides of the guide shaft mounting base 309. The movable locking tongue 305 is connected via a locking mechanism. The tongue guide shaft 304 is guided and oriented slidingly mounted on the right side of the fluid pool platform fixing seat 101. Multiple slots are provided on the upper surface of the movable locking tongue 305 along the moving direction. The slots are used to insert the anti-push block 303. When the movable locking tongue 305 moves to the designated position, the anti-push block 303 is inserted into the slot at the corresponding position. The position of the movable locking tongue 305 can be positioned by the blocking effect of the anti-push block 303. A crown spring female pin 306 is installed inside the movable locking tongue 305. A crown spring locking pad 307 is installed on the inner side of the movable locking tongue 305. The front end of the crown spring female pin 306 is limited and fixed by the crown spring locking pad 307. A buffer rubber pad 308 is installed on the front side of the crown spring locking pad 307.

[0027] The main function of this part is to clamp and fix the fluid pool assembly 2. By rotating the rotary button 301, the movable locking tongue 305 will move away from or towards the fluid pool along the horizontal axis. When moving towards the fluid pool, the movable locking tongue 305 approaches and abuts against the fluid pool, while the front buffer rubber pad 308 abuts against the fluid pool assembly 2 and locks it. The movable locking tongue 305 is restricted by the locking tongue guide shaft 304, limiting its degree of freedom and allowing it to slide horizontally in a straight line along the track without swinging back and forth. When the buffer rubber pad 308 is firmly pressed against the right side... After the fluid pool 22 is inserted, the anti-push block 303 is inserted into the corresponding slot to position the movable locking tongue 305. The positioning of the movable locking tongue 305 can lock the fluid pool component 2. The clamping force can be determined by aligning the anti-push block with the same slot each time. The locking and fixing by the movement of the movable locking tongue 305 eliminates the cumbersome process of locking screws, greatly improving the user's operating efficiency and experience. In addition, the buffer rubber pad 308 can provide buffering force in the middle, providing buffer protection for the fluid pool component 2 and the chip, so as not to make abnormal noises or uneven force that could cause the chip to be crushed.

[0028] In practical use, the nanopore processing fluid pool platform of this utility model first assembles the chip 5 to be processed into the left and right fluid pools, and pre-inserts conductive silver wires 201 on both sides of the two fluid pools. Then, the prepared fluid pool assembly 2 is placed on the positioning platform on the fluid pool platform 1, so that the left fluid pool 21 abuts against the fluid pool platform 1. At the same time, the conductive silver wires 201 that have been threaded into the left fluid pool 21 are coaxially and horizontally inserted into and abut against the left fixed side crown spring pin 103 of the fluid pool platform fixing seat 101, so that the left fluid pool 21 is connected to the equipment and connected to the external power supply. On the other side, it is necessary to rotate the rotary button 301. Rotating the rotary button 301 drives the movable locking tongue 305 to move linearly, so that the movable locking tongue 305 moves away from or near the fluid pool assembly 2. When it moves near the fluid pool assembly 2, the front side of the buffer rubber pad 308 at the front end of the movable locking tongue 305 first contacts and presses against the right fluid pool 22. At the same time, when the rotary button 301 is operated, the conductive silver wire 201 that has been threaded on the right fluid pool 22 is coaxially inserted into the crown spring female needle 306 and abuts against it, so that the right fluid pool 22 is connected to the device and connected to the external power supply. The position of the movable locking tongue is then positioned by the anti-push block. Finally, electrolyte solution is dripped into the electrolyte holes 204 of the left fluid pool 21 and the right fluid pool 22, and the fluid pools on both sides are connected to form a liquid-solid-liquid working environment. The entire nanopore processing instrument stage enters the nanopore drilling state.

[0029] The above-mentioned connection to the external power source includes, but is not limited to, the crown spring female pin. This embodiment uses the crown spring female pin as an example, but it can also be a spring pin or a magnetic connector, etc.

[0030] With the use of the fluid pool and fluid pool platform described above, operators can easily get started with simple training. The low success rate of nanopore drilling due to uneven operator skill levels or improper operation can be effectively improved, thus enhancing work efficiency and product preparation. It is simple, easy to use, and user-friendly. Example

[0031] Based on Example 1, such as Figure 2 As shown, a micro switch sensing component 4 is installed on the fluid pool platform 1. The micro switch sensing component 4 mainly consists of a micro switch 401 and a micro switch bracket 402. The micro switch bracket 402 is fixed to one side of the fluid pool platform 1, and the micro switch 401 is installed on the micro switch bracket 402 and connected to the circuit board of the equipment. The main function of setting up the micro switch 401 sensing component 4 is that when the fluid pool component 2 is placed in and removed from the fluid pool platform 1, the micro switch 401 will be triggered to emit a voice prompt, which has a prompting and warning function for the operator. It can identify whether it has been placed in the fluid pool and whether it has been properly connected, which better reflects the design concept of human-machine interaction.

[0032] The above description is only a preferred embodiment of the present utility model and does not limit the present utility model. Any modifications, equivalent substitutions and improvements made within the spirit and principles of the present utility model should be included within the protection scope of the present utility model.

Claims

1. A nanopore processing fluid cell stage comprising a fluid cell stage (1), a fluid cell assembly (2) and a fluid cell clamping assembly (3), characterised in that: The fluid pool assembly (2) is composed of two mutually butted left fluid pool (21) and right fluid pool (22), the fluid pool carrier (1) is composed of fluid pool carrier fixing seat (101), crown spring lock seat (102), crown spring female needle one (103), crown spring lock gasket one (104), the fluid pool carrier fixing seat (101) is U-shaped structure, the crown spring female needle one (103) is installed on the left side of the fluid pool carrier fixing seat (101) through the crown spring lock seat (102) and the crown spring lock gasket one (104); The fluid pool clamping assembly (3) includes the rotary button fixing seat (302) fixed on the right side of the fluid pool carrier fixing seat (101) upper, the rotary button fixing seat (302) and the fluid pool carrier fixing seat (101) are axially slidably sleeved with the movable lock tongue (305), the movable lock tongue (305) is installed with the crown spring female needle two (306) through the crown spring lock gasket two (307) in, and the buffer rubber pad (308) is installed on the front side of the crown spring lock gasket two (307).

2. A nanopore processing fluid cell stage according to claim 1, wherein: Solution channels are formed in the left fluid pool (21) and the right fluid pool (22), electrolyte holes (204) are formed in the top of the two fluid pools and communicate with the solution channels, nylon columns (202) are sleeved on the outer sides of the two fluid pools, sealing washers (203) are arranged at the connection of the inner ends of the nylon columns (202) and the solution channels, conductive silver wires (201) are inserted into the nylon columns (202), the front ends of the conductive silver wires (201) are inserted into the solution channels, and sealing gaskets (205) are detachably arranged at the inner side butt joints of the two fluid pools.

3. A nanopore processing fluid cell stage according to claim 1, wherein: The right rear of the fluid pool carrier fixing seat (101) is fixed with a guide shaft fixing seat (309), a plurality of lock tongue guide shafts (304) are axially arranged on the guide shaft fixing seat (309), and the movable lock tongue (305) is slidably sleeved on the right side of the fluid pool carrier fixing seat (101) through the lock tongue guide shaft (304).

4. A nanopore processing fluid cell stage according to claim 1, wherein: A plurality of insertion slots are formed in the upper surface of the movable lock tongue (305) along the moving direction, the insertion slots are used for inserting the prevention push block (303), and the prevention push block (303) is positioned with the insertion slot.

5. A nanopore processing fluid cell stage according to claim 1, wherein: A microswitch sensing assembly (4) is installed on the fluid pool carrier (1), the microswitch sensing assembly (4) is composed of a microswitch (401) and a microswitch bracket (402), the microswitch bracket (402) is fixed on one side of the fluid pool carrier (1), and the microswitch (401) is installed on the microswitch bracket (402).

6. A nanopore processing fluid cell stage according to claim 1, wherein: An overflow hole (206) is formed in the fluid pool assembly (2), and the overflow hole (206) communicates with the solution channel.

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