Kit applicable to field fish identification
By integrating a refrigeration system and designing universal primers into the field kit, the problems of sample preservation and identification efficiency were solved, achieving the effects of low-temperature preservation and rapid identification, and simplifying the field species identification process.
Patent Information
- Application Number
- CN202520433608.7
- Authority / Receiving Office
- CN · China
- Patent Type
- Utility models(China)
- Current Assignee / Owner
- Filing Date
- 2025-03-13
- Publication Date
- 2026-01-02
- Estimated Expiration
- 2035-03-13
AI Technical Summary
Existing species identification kits cannot effectively preserve samples under low-temperature conditions, affecting the freshness of samples for field workers. Furthermore, the PCR amplification and sequencing processes are time-consuming and costly, making it difficult to meet the needs of rapid identification in the field.
A kit with a miniature refrigeration compressor, condenser, and insulation layer was designed. Using universal primers designed on 18S rDNA and 5.8S rDNA, the ITS1 and ITS2 sequences were amplified in one step, and species were directly identified by gel electrophoresis, avoiding the sequencing process.
This technology enables the preservation of sample freshness at low temperatures in the field, simplifies the identification process, saves costs, and improves work efficiency.
Smart Images
Figure CN223751557U_ABST
Abstract
Description
TECHNICAL FIELD
[0001] The utility model relates to biological species identification technical field, concretely relates to a reagent box suitable for field fish identification. BACKGROUND
[0002] Molecular biology method extracts species DNA, carries out PCR sequencing, and identifies after comparing with a database; compared with morphological method, molecular biology means low threshold, easy to start, and can identify species in batches. Current commonly used molecular identification means is based on DNA bar code technology, and the mitochondrial COI gene of these species is amplified through PCR method, and then the gene is sequenced, and species identification is carried out through sequence comparison. The maintenance of sample freshness is the difficult problem that field workers often encounter, under the condition of lacking refrigeration equipment, the usual practice is to fix sample tissue in alcohol solution or other solution, but the method can affect subsequent operation and use, such as damaging the integrity of DNA, and then affecting the quality of genome. Current species identification reagent box developed based on molecular biology mostly operates at normal temperature, mainly aims at indoor staff, and cannot meet the needs of field staff who need to store samples at low temperature. The existing technology has the following main problems:
[0003] Because the existing species identification reagent box is only suitable for indoor normal temperature operation, it cannot effectively solve the sample needing to be stored at low temperature, field workers cannot guarantee the freshness of samples due to lack of effective refrigeration equipment; meanwhile, the reagent box needs to be subjected to PCR amplification and sequencing after extracting DNA, which is high in cost and time-consuming, and the above are not conducive to field workers to collect fresh samples and rapidly identify species. UTILITY MODEL CONTENT
[0004] The utility model provides a reagent box suitable for field fish identification to solve the problems in the background art.
[0005] To solve the above technical problems, the technical scheme adopted by the utility model is:
[0006] The utility model provides a kit suitable for field fish identification, comprising an identification kit, a sealing cover hinged to the top of the identification kit, a portable handle fixedly installed on the top of the sealing cover, a spring snap fastener arranged on the front side of the identification kit, an ultraviolet illumination mechanism arranged on the left side of the identification kit, a refrigeration equipment shell fixedly installed on the rear side of the identification kit, a mobile power supply fixedly installed on the top of the refrigeration equipment shell, a miniature refrigeration compressor fixedly installed in the right side of the inner cavity of the refrigeration equipment shell, a condenser fixedly installed in the left side of the inner cavity of the refrigeration equipment shell, a heat preservation layer fixedly installed on the inner wall of the identification kit, a refrigeration layer fixedly installed on the inner wall of the heat preservation layer, the output end of the miniature refrigeration compressor penetrating into the inner cavity of the identification kit and being fixedly connected with the input end of the refrigeration layer, a partition plate fixedly installed between the front and rear sides of the inner wall of the refrigeration layer, an identification platform fixedly installed on the left side of the partition plate, and an identification plate arranged on the top of the identification platform, and a test tube storage mechanism arranged on the right side of the partition plate.
[0007] Further improvement of the utility model technical scheme lies in that the bottom of the sealing cover is fixedly installed with a sealing clamping plate, the outer wall of the sealing clamping plate is fixedly bonded with a rubber ring, the bottom of the sealing clamping plate is provided with a storage groove, the inner side of the storage groove is provided with a placing bag, and the bottom of the sealing clamping plate is overlapped with the top of the partition plate.
[0008] Further improvement of the utility model technical scheme lies in that the test tube storage mechanism comprises a test tube placing plate, a plurality of jack holes are arranged in the top of the test tube placing plate in a rectangular array, pull handles are fixedly installed on the top of the test tube placing plate on the front and rear sides, sample test tubes are clamped in the inner circle of the plurality of jack holes, and the bottom of the sealing clamping plate is overlapped with the top of the two pull handles.
[0009] Further improvement of the utility model technical scheme lies in that the bottom of the test tube placing plate is fixedly installed with four hangers in a rectangular array, a placing bottom plate is fixedly installed at the bottom end of the four hangers, a plurality of test tube placing grooves are arranged in the top of the placing bottom plate in a rectangular array, the plurality of test tube placing grooves are aligned with the plurality of jack holes in an up-down direction, and the bottom end of the plurality of sample test tubes is clamped with the plurality of test tube placing grooves respectively.
[0010] Further improvement of the utility model technical scheme lies in that a plurality of sequence length display grooves are arranged in the top of the identification plate, power supply wires are electrically connected to the rear side of the identification plate, and the identification plate is powered by an external mobile power supply through the power supply wires.
[0011] The further improvement of the technical scheme of the utility model lies in that the ultraviolet lighting mechanism comprises a fixed side plate, the fixed side plate is fixedly installed at the left side of the identification kit, a lamp holder is fixedly installed at the top of the fixed side plate, a universal hose is fixedly installed at the top of the lamp holder, and an ultraviolet lighting lamp is fixedly installed at the top end of the universal hose.
[0012] Due to the adoption of the above technical scheme, the utility model has the following technical progress compared with the prior art:
[0013] 1. The reagent kit suitable for field fish identification is provided, the micro refrigeration compressor, the condenser, the portable power source, the heat preservation layer, the refrigeration layer and the test tube storage mechanism are mutually matched, so that the low-temperature storage environment is kept in the whole reagent kit, the demand of low-temperature sample preservation is met, and the freshness of the sample is ensured.
[0014] 2. The reagent kit suitable for field fish identification is provided, the universal primer is designed on the 18S rDNA and 5.8S rDNA at two ends and on the 5.8S rDNA and 28S rDNA, the ITS1 and ITS2 sequences of a batch of common fishes are amplified at one time, the species identification is directly carried out by comparing the lengths of the ITS sequences of different species, so that the sequencing process is avoided, the cost is saved, and more importantly, the work efficiency is greatly improved. DETAILED DESCRIPTION
[0015] Figure 1 It is the whole schematic view of the utility model structure;
[0016] Figure 2 It is the rear view schematic view of the identification kit of the utility model structure;
[0017] Figure 3 It is the schematic view of the sealing cover opening state of the utility model structure;
[0018] Figure 4 It is the schematic view of the test tube storage mechanism of the utility model structure;
[0019] Figure 5 It is the schematic view of the ultraviolet lighting mechanism of the utility model structure.
[0020] In the figure: 1, identification kit; 11, refrigeration equipment shell; 111, miniature refrigeration compressor; 112, condenser; 113, mobile power supply; 12, heat preservation layer; 13, refrigeration layer; 14, partition plate; 141, identification platform; 15, test tube storage mechanism; 151, test tube placement plate; 152, jack; 153, pull handle; 154, sample test tube; 155, boom; 156, placement bottom plate; 157, test tube placement groove; 16, identification plate; 161, sequence length display groove; 162, power supply wire; 2, sealing cover; 21, sealing clamping plate; 211, rubber ring; 22, storage groove; 221, placement bag; 3, portable handle; 4, spring buckle; 5, ultraviolet illumination mechanism; 51, fixed side plate; 52, lamp holder; 53, universal hose; 54, ultraviolet illumination lamp. DETAILED DESCRIPTION
[0021] In order to make the technical means, creative features, purposes and effects realized by the utility model easy to understand, the utility model is further described below in combination with specific embodiments.
[0022] As shown in Figure 1 , Figure 2 , Figure 3 , the utility model provides a reagent box suitable for field fish identification, including identification kit 1, the top of identification kit 1 is hinged with sealing cover 2, the top of sealing cover 2 is fixedly installed with portable handle 3, the front side of identification kit 1 is provided with spring buckle 4, the left side of identification kit 1 is provided with ultraviolet illumination mechanism 5, the rear side of identification kit 1 is fixedly installed with refrigeration equipment shell 11, the top of refrigeration equipment shell 11 is fixedly installed with mobile power supply 113, the right side in the cavity of refrigeration equipment shell 11 is fixedly installed with miniature refrigeration compressor 111, the left side in the cavity of refrigeration equipment shell 11 is fixedly installed with condenser 112, the inner wall of identification kit 1 is fixedly installed with heat preservation layer 12, the inner wall of heat preservation layer 12 is fixedly installed with refrigeration layer 13, the output end of miniature refrigeration compressor 111 is fixedly connected with the input end of refrigeration layer 13 and is connected to the inner cavity of identification kit 1, the inner wall of refrigeration layer 13 is fixedly installed with partition plate 14 between the front and rear sides, the left side of partition plate 14 is fixedly installed with identification platform 141, the top of identification platform 141 is provided with identification plate 16, the right side of partition plate 14 is provided with test tube storage mechanism 15, the bottom of sealing cover 2 is fixedly installed with sealing clamping plate 21, the outer wall of sealing clamping plate 21 is fixedly bonded with rubber ring 211, the bottom of sealing clamping plate 21 is provided with storage groove 22, the inner side of storage groove 22 is provided with placement bag 221, the bottom of sealing clamping plate 21 is overlapped with the top of partition plate 14, and sealing clamping plate 21 is connected with the inner cavity of refrigeration layer 13.
[0023] When in use, the portable handle 3 on the top of the sealing cover 2 can be used to carry the whole identification kit 1, and the sealing cover 2 can be opened by opening the spring buckle 4 when it is needed to store the fish sample. Meanwhile, the metal material refrigeration layer 13 in the inner cavity of the whole identification kit 1 can supply power to the micro refrigeration compressor 111 and the condenser 112 in the inner cavity of the refrigeration equipment shell 11 through the portable power supply 113, so as to carry out refrigeration, so that the inner cavity of the whole refrigeration layer 13 is in a low-temperature environment, and the heat preservation layer 12 of rock wool material is used for heat preservation. When the sealing cover 2 is closed, the sealing clamping plate 21 can be clamped into the inner cavity of the refrigeration layer 13, and the sealing is completed by using the rubber ring 211, so as to further ensure the low-temperature environment in the inner cavity of the identification kit 1, so that the sample storage effect is better. Meanwhile, the gel and dyeing agent used for identification and other materials can be stored by placing the bag 221.
[0024] As shown in Figure 4 , the test tube storage mechanism 15 comprises a test tube placing plate 151, a plurality of insertion holes 152 are arranged in a rectangular array on the top of the test tube placing plate 151, pull handles 153 are fixedly installed on the top of the test tube placing plate 151, sample test tubes 154 are clamped in the inner circle of the insertion holes 152, the bottom of the sealing clamping plate 21 is overlapped with the top of the pull handles 153, four hanging rods 155 are fixedly installed on the bottom of the test tube placing plate 151, a placing bottom plate 156 is fixedly installed on the bottom of the four hanging rods 155, a plurality of test tube placing grooves 157 are arranged in a rectangular array on the top of the placing bottom plate 156, the plurality of test tube placing grooves 157 are aligned with the plurality of insertion holes 152, and the bottom of the plurality of sample test tubes 154 is clamped with the plurality of test tube placing grooves 157 respectively;
[0025] Different field fish can be collected by collecting the body surface mucus into the sample test tube 154, and the sample test tube 154 is clamped into the insertion hole 152 of the test tube placing plate 151, and is clamped into the test tube placing groove 157 on the top of the placing bottom plate 156 until the bottom is fixed. Then the whole test tube placing plate 151 can be taken up by means of the hanging rod 155 and the pull handle 153, and is clamped into the right side area of the partition plate 14, so as to be completely placed in the inner cavity of the refrigeration layer 13, which is convenient for centralized taking or placing.
[0026] As shown in Figure 5 , a plurality of sequence length display grooves 161 are arranged on the top of the identification plate 16, and power supply wires 162 are electrically connected to the rear side of the identification plate 16. The identification plate 16 is powered by the portable power supply 113 through the power supply wires 162. The ultraviolet illumination mechanism 5 comprises a fixed side plate 51, which is fixedly installed on the left side of the identification kit 1. A lamp holder 52 is fixedly installed on the top of the fixed side plate 51. A universal hose 53 is fixedly installed on the top of the lamp holder 52. An ultraviolet illuminating lamp 54 is fixedly installed on the top end of the universal hose 53.
[0027] When identification is needed, one of the sample test tubes 154 is taken out, and the gel is placed in the sequence length display groove 161 opened at the top of the identification plate 16, then the sample in the sample test tube 154 is placed on the gel in the sequence length display groove 161, and then the identification plate 16 is powered by the external power supply 113 connected by the power supply wire 162, so that the sample DNA runs in the gel, after a period of time, the fluorescent agent is added for staining, and the ultraviolet illuminating lamp 54 is bent above the identification plate 16 by opening the lamp holder 52 powered by the external power supply 113 and using the universal hose 53, so that the sample is colored, so as to judge the field fish species according to the sequence length of the sample. The ribosomal internal transcribed spacer (ITS1 and ITS2) evolution rate is relatively fast, basically similar within the species, and significantly different between the species, and the length of the internal transcribed spacer of different species is very different, so the length heterogeneity can be directly observed by gel electrophoresis with the naked eye to identify the species. Through PCR amplification and gel electrophoresis to display the sequence length, the common species can be identified, avoiding the need for sequencing of the conventional kit, saving time and cost.
[0028] The working principle of the kit for identifying field fish will be described in detail below.
[0029] As Figures 1-5As shown, when in use, the portable handle 3 on the top of the sealing cover 2 can be used to carry the whole identification kit 1, and when it is needed to store the fish sample, the sealing cover 2 can be opened by opening the spring buckle 4, and meanwhile, the metal material refrigeration layer 13 in the inner cavity of the whole identification kit 1 can supply power to the micro refrigeration compressor 111 and the condenser 112 in the inner cavity of the refrigeration equipment shell 11 through the portable power supply 113, so as to carry out refrigeration, so that the inner cavity of the whole refrigeration layer 13 is in a low-temperature environment, and the heat preservation layer 12 of rock wool material is used for heat preservation, and when the sealing cover 2 is closed, the sealing clamping plate 21 can be clamped into the inner cavity of the refrigeration layer 13, and the rubber ring 211 is used to complete the sealing, so as to further ensure the low-temperature environment in the inner cavity of the identification kit 1, so that the sample storage effect is better, and the gel and the staining agent used for identification and other materials can be stored by placing the bag 221, different field fish can be collected by collecting the body surface mucus into the sample test tube 154, and the sample test tube 154 is clamped into the insertion hole 152 of the test tube placing plate 151, and is clamped into the test tube placing groove 157 of the test tube placing plate 156 on the top of the test tube placing plate 156, and then the whole test tube placing plate 151 is lifted by the hanging rod 155 and the lifting handle 153, and is clamped into the right side area of the partition plate 14, so as to be completely placed in the inner cavity of the refrigeration layer 13, when identification is needed, one of the sample test tubes 154 is taken out, the gel is placed in the sequence length display groove 161 on the top of the identification plate 16, then the sample in the sample test tube 154 is placed on the gel in the sequence length display groove 161, then the identification plate 16 is powered by the portable power supply 113 connected to the power supply wire 162, so that the sample DNA can run in the gel, after a period of time, the fluorescent agent is added, the lamp holder 52 is powered by the portable power supply 113, the ultraviolet illuminating lamp 54 is bent above the identification plate 16 by the universal hose 53, so that the sample can be colored, so as to judge the field fish species according to the sequence length of the sample.
[0030] The above has made a detailed description of the present application, but some modifications or improvements can be made on the basis of the present application, which is obvious to those skilled in the art. Therefore, the modifications or improvements without departing from the spirit of the present application are within the scope of the present application.
Claims
1. A kit suitable for field use for fish identification, comprising an identification kit (1), characterized in that: The top of the identification kit (1) is hinged with a sealing cover (2), the top of the sealing cover (2) is fixedly installed with a portable handle (3), the front side of the identification kit (1) is provided with a spring buckle (4), the left side of the identification kit (1) is provided with an ultraviolet illumination mechanism (5), the rear side of the identification kit (1) is fixedly installed with a refrigeration equipment shell (11), the top of the refrigeration equipment shell (11) is fixedly installed with a mobile power supply (113), the right side of the inner cavity of the refrigeration equipment shell (11) is fixedly installed with a miniature refrigeration compressor (111), the left side of the inner cavity of the refrigeration equipment shell (11) is fixedly installed with a condenser (112), the inner wall of the identification kit (1) is fixedly installed with a heat preservation layer (12), the inner wall of the heat preservation layer (12) is fixedly installed with a refrigeration layer (13), the output end of the miniature refrigeration compressor (111) penetrates into the inner cavity of the identification kit (1) and is fixedly connected with the input end of the refrigeration layer (13), the inner wall of the refrigeration layer (13) is fixedly installed with a partition plate (14) between the front and rear sides, the left side of the partition plate (14) is fixedly installed with an identification platform (141), the top of the identification platform (141) is provided with an identification plate (16), the right side of the partition plate (14) is provided with a test tube storage mechanism (15).
2. The kit for field identification of fish according to claim 1, characterized in that: The bottom of the sealing cover (2) is fixedly installed with a sealing clamping plate (21), the outer wall of the sealing clamping plate (21) is fixedly bonded with a rubber ring (211), the bottom of the sealing clamping plate (21) is provided with a storage groove (22), the inner side of the storage groove (22) is provided with a placing bag (221), the bottom of the sealing clamping plate (21) is overlapped with the top of the partition plate (14), and the sealing clamping plate (21) is clamped with the inner cavity of the refrigeration layer (13).
3. The kit for field identification of fish according to claim 2, characterized in that: The test tube storage mechanism (15) comprises a test tube placing plate (151), a plurality of insertion holes (152) are arranged in a rectangular array on the top of the test tube placing plate (151), pull handles (153) are fixedly installed on the top of the test tube placing plate (151) on the front and rear sides, a plurality of sample test tubes (154) are clamped in the inner circle of the insertion holes (152), and the bottom of the sealing clamping plate (21) is overlapped with the top of the two pull handles (153).
4. The kit for field identification of fish according to claim 3, characterized in that: Four hangers (155) are fixedly installed in a rectangular array on the bottom of the test tube placing plate (151), a placing bottom plate (156) is fixedly installed at the bottom end of the four hangers (155), a plurality of test tube placing grooves (157) are arranged in a rectangular array on the top of the placing bottom plate (156), the plurality of test tube placing grooves (157) are aligned with the plurality of insertion holes (152) up and down, and the bottom end of the plurality of sample test tubes (154) is clamped with the plurality of test tube placing grooves (157) respectively.
5. The kit for field identification of fish species as claimed in claim 1, wherein: A plurality of sequence length display grooves (161) are arranged on the top of the identification plate (16), power supply wires (162) are electrically connected to the rear side of the identification plate (16), and the identification plate (16) is powered by the external mobile power supply (113) through the power supply wires (162).
6. The kit for field identification of fish species according to claim 1, characterized in that: The ultraviolet illumination mechanism (5) comprises a fixed side plate (51) fixedly installed on the left side of the identification kit (1), a lamp holder (52) fixedly installed on the top of the fixed side plate (51), a universal hose (53) fixedly installed on the top of the lamp holder (52), and an ultraviolet illumination lamp (54) fixedly installed on the top end of the universal hose (53).