Dropping liquid driving device and digital PCR (Polymerase Chain Reaction) detection equipment

Abstract

The utility model discloses a dropping liquid driving device and digital PCR (Polymerase Chain Reaction) detection equipment, and relates to the technical field of nucleic acid detection. The liquid dropping driving device comprises a rack, a first driving mechanism, a mounting frame, a push-pull mechanism and a liquid dropping injector. The first driving mechanism is installed on the machine frame and connected with the installation frame, the liquid dropping injector comprises a needle tube and a push rod, the push rod is arranged in the needle tube in a sliding mode, the needle tube is connected with the installation frame, the push-pull mechanism is installed on the installation frame and connected with the push rod, and the first driving mechanism is used for synchronously driving the push-pull mechanism and the liquid dropping injector to move through the installation frame. The push-pull mechanism is used for driving the push rod to slide relative to the needle tube. The liquid dropping driving device provided by the utility model can realize automatic liquid dropping operation, improve the liquid dropping efficiency, improve the liquid dropping amount precision, save the labor cost and the time cost, and ensure the nucleic acid detection efficiency and the nucleic acid detection effect.

Description

TECHNICAL FIELD

[0001] The utility model relates to nucleic acid detection technical field, specifically, relate to a kind of drop driving device and digital PCR detection equipment. BACKGROUND

[0002] Microdroplet digital PCR is an absolute quantitative PCR technique, which mainly uses microdroplet method to disperse the solution containing target nucleic acid into a large number of water-in-oil droplets, forming tens of thousands of independent micro-reaction systems with equal volume. The initial number of target nucleic acid templates in each droplet is 0, 1 or more. After PCR cycle, the droplet with an initial target nucleic acid will give a fluorescence signal, and the droplet without target nucleic acid will not give a fluorescence signal. According to the relative proportion of the two kinds of droplets and the mathematical correction of Poisson distribution, the concentration of the original solution of target nucleic acid can be calculated.

[0003] Microdroplet digital PCR requires thermal cycling (using a temperature control device to cycle the droplets up and down). The current digital PCR detection equipment is manually dropped a certain amount of droplets into the reaction disc, and then the reaction disc is placed on the heat conduction surface of the temperature control device. The temperature control device controls the temperature of the droplets through the reaction disc. However, this method is time-consuming and labor-intensive, with high labor and time costs, low droplet efficiency, low droplet volume accuracy, and direct impact on nucleic acid detection efficiency and nucleic acid detection effect.

[0004] Therefore, it is particularly important to design and manufacture a droplet driving device with high droplet efficiency and high droplet volume accuracy, especially in nucleic acid detection. SUMMARY

[0005] The utility model aims at providing a kind of drop driving device, can realize automatic liquid drop operation, improve droplet efficiency, improve droplet volume accuracy, save labor cost and time cost, guarantee nucleic acid detection efficiency and nucleic acid detection effect.

[0006] Another purpose of the utility model is to provide a kind of digital PCR detection equipment, which can realize automatic liquid drop operation, improve droplet efficiency, improve droplet volume accuracy, save labor cost and time cost, guarantee nucleic acid detection efficiency and nucleic acid detection effect.

[0007] The utility model is realized by using the following technical solutions.

[0008] The application discloses a kind of drop driving device, including frame, first driving mechanism, mounting frame, push-pull mechanism and drop injector, first driving mechanism is installed in frame, and with mounting frame is connected, drop injector includes needle tube and push rod, push rod is slidably arranged in needle tube, needle tube is connected with mounting frame, push-pull mechanism is installed in mounting frame, and with push rod is connected, first driving mechanism is used to drive push-pull mechanism and drop injector movement by mounting frame synchronously, push-pull mechanism is used to drive push rod to slide relative to needle tube.

[0009] Optionally, the first driving mechanism includes a first driving assembly, a translation frame and a second driving assembly, the first driving assembly is installed on the frame and connected with the translation frame, the first driving assembly is used to drive the translation frame to move in a first direction, the second driving assembly is installed on the translation frame and connected with the mounting frame, the second driving assembly is used to drive the mounting frame to move up and down in a second direction, the first direction is perpendicular to the second direction.

[0010] Optionally, the first driving assembly includes a first driving member, a first screw rod and a first nut, the first driving member is connected with the first screw rod, the first nut is sleeved outside the first screw rod and threadedly matched with the first screw rod, the first nut is connected with the translation frame, and the axial direction of the first screw rod is the first direction.

[0011] Optionally, the frame is provided with a first guide rail, the translation frame is provided with a first sliding block, the first sliding block is slidably matched with the first guide rail, and the extension direction of the first guide rail is the first direction.

[0012] Optionally, the second driving assembly includes a second driving member, a second screw rod and a second nut, the second driving member is connected with the second screw rod, the second nut is sleeved outside the second screw rod and threadedly matched with the second screw rod, the second nut is connected with the mounting frame, and the axial direction of the second screw rod is the second direction.

[0013] Optionally, the drop driving device further includes a second driving mechanism and a bearing table, the second driving mechanism is installed on the frame and connected with the bearing table, the second driving mechanism is used to drive the bearing table to move in a third direction, the first direction, the second direction and the third direction are perpendicular to each other.

[0014] Optionally, the second driving mechanism includes a third driving member, a third screw rod and a third nut, the third driving member is connected with the third screw rod, the third nut is sleeved outside the third screw rod and threadedly matched with the third screw rod, the third nut is connected with the bearing table, and the axial direction of the third screw rod is the third direction.

[0015] Optionally, the push-pull mechanism includes a fourth driving member, a fourth screw rod and a fourth nut, the fourth driving member is connected with the fourth screw rod, the fourth nut is sleeved outside the fourth screw rod and threadedly matched with the fourth screw rod, the fourth nut is connected with the push rod, and the axial direction of the fourth screw rod is the same as the extension direction of the needle tube.

[0016] Optionally, the needle tube and the push rod are arranged to extend in a vertical direction, and the push-pull mechanism is connected to a top of the push rod.

[0017] The digital PCR detection device comprises the droplet driving device, and the digital PCR detection device can realize automatic droplet operation, improve droplet efficiency, improve droplet quantity precision, save labor cost and time cost, and ensure nucleic acid detection efficiency and nucleic acid detection effect.

[0018] The droplet driving device and the digital PCR detection device have the following beneficial effects:

[0019] The digital PCR detection device comprises the droplet driving device, and the digital PCR detection device can realize automatic droplet operation, improve droplet efficiency, improve droplet quantity precision, save labor cost and time cost, and ensure nucleic acid detection efficiency and nucleic acid detection effect.

[0020] The digital PCR detection device comprises the droplet driving device, and the digital PCR detection device can realize automatic droplet operation, improve droplet efficiency, improve droplet quantity precision, save labor cost and time cost, and ensure nucleic acid detection efficiency and nucleic acid detection effect. BRIEF DESCRIPTION OF DRAWINGS

[0021] In order to more clearly illustrate the technical scheme of the embodiments of the present application, the following will briefly introduce the drawings needed to be used in the embodiments, and it should be understood that the following drawings only show some embodiments of the present application, and therefore should not be regarded as a limitation to the scope, and for those skilled in the art, other related drawings can also be obtained without creative labor on the basis of these drawings.

[0022] Figure 1 The structure schematic view of the digital PCR detection device provided by the present application is shown in the figure.

[0023] Figure 2 The structure schematic view of the connection between the push-pull mechanism and the droplet injector in the droplet driving device provided by the present application is shown in the figure.

[0024] Figure 3 A cross-sectional view of the drip liquid injector of the drip liquid driving device is provided for the embodiments of the utility model;

[0025] Figure 4 A structure schematic view of one perspective of the drip liquid driving device is provided for the embodiments of the utility model;

[0026] Figure 5 A structure schematic view of another perspective of the drip liquid driving device is provided for the embodiments of the utility model.

[0027] Icon: 10 - digital PCR detection equipment;100 - drip liquid driving device;110 - rack;111 - first guide rail;112 - third guide rail;120 - first driving mechanism;121 - first driving assembly;1211 - first driving piece;1212 - first screw;1213 - first nut;122 - translation frame;1221 - first sliding block;1222 - second guide rail;123 - second driving assembly;1231 - second driving piece;1232 - second screw;1233 - second nut;130 - mounting frame;131 - second sliding block;132 - fourth guide rail;140 - push-pull mechanism;141 - fourth driving piece;142 - fourth screw;143 - fourth nut;144 - fourth sliding block;150 - drip liquid injector;151 - needle tube;152 - push rod;160 - second driving mechanism;161 - third driving piece;162 - third screw;163 - third nut;170 - bearing table;171 - third sliding block;200 - temperature control device;300 - reaction disc. DETAILED DESCRIPTION

[0028] In order to make the purpose, technical scheme and advantages of the embodiments of the utility model clearer, the technical scheme in the embodiments of the utility model will be described clearly and completely below in combination with the drawings in the embodiments of the utility model. Obviously, the described embodiments are part of the embodiments of the utility model, rather than all the embodiments. The components of the embodiments of the utility model described and shown in the drawings here can be arranged and designed in various different configurations.

[0029] Therefore, the following detailed description of the embodiments of the utility model provided in the drawings is not intended to limit the scope of the claimed utility model, but only represents selected embodiments of the utility model. Based on the embodiments in the utility model, all other embodiments obtained by those of ordinary skill in the art without creative labor fall within the scope of protection of the utility model.

[0030] It should be noted that like reference numerals and letters refer to like items in the several views, and that once an item is defined in one view, it should not require further defining and explaining in subsequent views.

[0031] In the description of the utility model, it needs to be explained that the directions or position relations indicated by the terms "inner", "outer", "upper", "lower", "horizontal" and the like are based on the directions or position relations shown in the drawings, or are the directions or position relations in which the utility model product is usually placed during use, and are only for the convenience of describing the utility model and simplifying the description, and do not indicate or imply that the devices or elements referred to must have a particular orientation, be constructed and operated in a particular orientation, and therefore cannot be understood as limiting the utility model. In addition, the terms "first", "second", "third" and the like are only used for differentiation in description and cannot be understood as indicating or implying relative importance.

[0032] In the description of the utility model, it also needs to be explained that, unless otherwise explicitly specified and limited, the terms "setting", "connecting", "installing" and "connecting" should be understood broadly, for example, can be fixedly connected, or can be detachably connected, or integrally connected; can be mechanically connected, or can be electrically connected; can be directly connected, or can be indirectly connected through an intermediate medium; or can be the communication inside two elements. For ordinary skilled in the art, the specific meanings of the above terms in the utility model can be understood according to specific circumstances.

[0033] Some embodiments of the utility model will be described in detail below with reference to the drawings. In the case of no conflict, the features in the following examples can be combined with each other.

[0034] Please refer to Figure 1 The utility model embodiment provides a kind of digital PCR detection equipment 10 for carrying out nucleic acid detection.It can realize automatic liquid drop operation, improve liquid drop efficiency, improve liquid drop quantity precision, save labor cost and time cost, guarantee nucleic acid detection efficiency and nucleic acid detection effect.

[0035] The digital PCR detection device 10 comprises a droplet driving device 100, a temperature control device 200 and a microscopic shooting device (not shown in the figure). The droplet driving device 100 is used to make a large number of water-in-oil droplets (to-be-tested droplets) from the original solution in a sample and inject the to-be-tested droplets into a reaction disc 300 placed on the temperature control device 200; the temperature control device 200 is used to perform a temperature rising and falling cycle on the to-be-tested droplets through the reaction disc 300, and the number of target nucleic acids in each to-be-tested droplet is doubled after each temperature rising and falling cycle, and the process is repeated to greatly increase the number of trace target nucleic acids; the microscopic shooting device is used to shoot and calculate all the to-be-tested droplets after the temperature rising and falling cycle is completed, and the to-be-tested droplets with target nucleic acids will give a fluorescent signal, and the to-be-tested droplets without target nucleic acids will not give a fluorescent signal, and according to the relative proportion of the number of the two kinds of to-be-tested droplets and the Poisson distribution mathematical correction, the target nucleic acid concentration of the original solution in the corresponding sample can be calculated.

[0036] The droplet driving device 100 comprises a rack 110, a first driving mechanism 120, a mounting rack 130, a push-pull mechanism 140 and a droplet injector 150. The first driving mechanism 120 is mounted on the rack 110 and connected with the mounting rack 130, the push-pull mechanism 140 and the droplet injector 150 are both mounted on the mounting rack 130, the first driving mechanism 120 is used to drive the push-pull mechanism 140 and the droplet injector 150 to move synchronously through the mounting rack 130 to realize the displacement function of the droplet injector 150, so as to facilitate the movement of the droplet injector 150 to different positions. The push-pull mechanism 140 is connected with the droplet injector 150, and the push-pull mechanism 140 is used to drive the droplet injector 150 to suck the original solution in the sample, and the push-pull mechanism 140 is also used to drive the droplet injector 150 to drip out the original solution, in this process, the oil pump pumps out the oil and wraps the original solution droplet to form a water-in-oil droplet (to-be-tested droplet), so as to realize the function of injecting the to-be-tested droplet into the reaction disc 300.

[0037] Please refer to Figure 2 and Figure 3The drop injector 150 includes a needle tube 151 and a push rod 152. The push rod 152 is slidably arranged in the needle tube 151, and the push rod 152 is capable of sliding relative to the needle tube 151 to form negative pressure or positive pressure in the needle tube 151, thereby realizing the liquid suction function and the drop function. The needle tube 151 can guide and limit the push rod 152. Specifically, the needle tube 151 is connected with the mounting frame 130, the mounting frame 130 can fix the needle tube 151, the push-pull mechanism 140 is installed on the mounting frame 130 and connected with the push rod 152, and the push-pull mechanism 140 is used to drive the push rod 152 to slide relative to the needle tube 151, so as to realize the automatic action of the push rod 152 without manual operation. In this way, the first driving mechanism 120 and the push-pull mechanism 140 jointly act to enable the drop injector 150 to suck the original solution in the sample at one position and drop the to-be-tested liquid droplets to the reaction disc 300 at another position, realize the automatic drop operation, improve the drop efficiency, improve the drop amount accuracy, save the labor cost and time cost, and ensure the nucleic acid detection efficiency and the nucleic acid detection effect.

[0038] In the embodiment, the needle tube 151 and the push rod 152 are arranged in the vertical direction, the inlet and outlet of the needle tube 151 are located at the bottom thereof, the push-pull mechanism 140 is connected to the top of the push rod 152, and the push-pull mechanism 140 can drive the push rod 152 to slide upward relative to the needle tube 151 to realize the liquid suction function through the inlet and outlet. The push-pull mechanism 140 can also drive the push rod 152 to slide downward relative to the needle tube 151 to realize the drop function through the inlet and outlet.

[0039] Please refer to Figure 1 , Figure 4 and Figure 5 , the first driving mechanism 120 includes a first driving assembly 121, a translation frame 122 and a second driving assembly 123. The first driving assembly 121 is installed on the rack 110 and connected with the translation frame 122, and the first driving assembly 121 is used to drive the translation frame 122 to move in the first direction. The second driving assembly 123 is installed on the translation frame 122 and connected with the mounting frame 130, and the second driving assembly 123 is used to drive the mounting frame 130 to lift in the second direction. Specifically, the first driving assembly 121 can synchronously drive the push-pull mechanism 140 and the drop injector 150 to move in the first direction through the translation frame 122, the second driving assembly 123 and the mounting frame 130 in sequence, the second driving assembly 123 can synchronously drive the push-pull mechanism 140 and the drop injector 150 to lift in the second direction through the mounting frame 130, and the first direction is perpendicular to the second direction, so that the push-pull mechanism 140 and the drop injector 150 can move to any position on the plane where the first direction and the second direction are located, thereby improving the movement accuracy.

[0040] In this embodiment, the first direction is a horizontal direction, the second direction is a vertical direction, the plane where the first direction and the second direction are located is a vertical plane, and the first driving mechanism 120 can drive the push-pull mechanism 140 and the drop injector 150 to move to any position on the vertical plane, so as to facilitate liquid suction and drop injection operations.

[0041] The first driving assembly 121 comprises a first driving member 1211, a first lead screw 1212 and a first nut 1213. The first driving member 1211 is connected with the first lead screw 1212, the first nut 1213 is sleeved outside the first lead screw 1212 and threadedly matched with the first lead screw 1212, the first nut 1213 is connected with the translation frame 122, and the axial direction of the first lead screw 1212 is the first direction. Specifically, the first driving member 1211 is configured to drive the first lead screw 1212 to rotate, so that the first nut 1213 is displaced along the axial direction of the first lead screw 1212, thereby driving the translation frame 122 to move along the first direction.

[0042] Further, the rack 110 is provided with a first guide rail 111, the translation frame 122 is provided with a first sliding block 1221, the first sliding block 1221 is slidably matched with the first guide rail 111, the extension direction of the first guide rail 111 is the first direction, the first sliding block 1221 can slide relative to the first guide rail 111, and the first guide rail 111 can guide and limit the first sliding block 1221, so as to ensure the stability of the sliding of the translation frame 122 relative to the rack 110.

[0043] The second driving assembly 123 comprises a second driving member 1231, a second lead screw 1232 and a second nut 1233. The second driving member 1231 is connected with the second lead screw 1232, the second nut 1233 is sleeved outside the second lead screw 1232 and threadedly matched with the second lead screw 1232, the second nut 1233 is connected with the mounting frame 130, and the axial direction of the second lead screw 1232 is the second direction. Specifically, the second driving member 1231 is configured to drive the second lead screw 1232 to rotate, so that the second nut 1233 is displaced along the axial direction of the second lead screw 1232, thereby driving the mounting frame 130 to move along the second direction.

[0044] Further, the translation frame 122 is provided with a second guide rail 1222, the mounting frame 130 is provided with a second sliding block 131, the second sliding block 131 is slidably matched with the second guide rail 1222, the extension direction of the second guide rail 1222 is the second direction, the second sliding block 131 can slide relative to the second guide rail 1222, and the second guide rail 1222 can guide and limit the second sliding block 131, so as to ensure the stability of the sliding of the mounting frame 130 relative to the translation frame 122.

[0045] Preferably, the droplet driving device 100 further comprises a second driving mechanism 160 and a bearing table 170. The second driving mechanism 160 is mounted on the rack 110 and connected with the bearing table 170, the bearing table 170 is used for mounting the temperature control device 200, the reaction disc 300 is placed on the temperature control device 200, and the second driving mechanism 160 is used for driving the bearing table 170 to move in the third direction. Specifically, the second driving mechanism 160 can drive the temperature control device 200 and the reaction disc 300 to move in the third direction through the bearing table 170 synchronously, and the first direction, the second direction and the third direction are perpendicular to each other, so that the push-pull mechanism 140 and the droplet injector 150 can move to any position on the bearing table 170, and it is ensured that the droplet injector 150 can inject the droplet of the to-be-tested liquid into the reaction disc 300.

[0046] Further, the number of the temperature control device 200 and the reaction disc 300 is multiple, multiple temperature control devices 200 are arranged side by side on the bearing table 170, and each reaction disc 300 is placed on one temperature control device 200. The first driving mechanism 120 and the second driving mechanism 160 jointly act to enable the droplet injector 150 to inject the droplet of the to-be-tested liquid of different samples into different reaction discs 300, so as to facilitate the nucleic acid detection of multiple samples at the same time and improve the detection efficiency. In addition, the multiple temperature control devices 200 are independently controlled, so as to facilitate flexible operation and further improve the detection efficiency.

[0047] In the embodiment, the number of the temperature control device 200 and the reaction disc 300 is four, but is not limited thereto. In other embodiments, the number of the temperature control device 200 and the reaction disc 300 can be two or eight, and the number of the temperature control device 200 and the reaction disc 300 is not specifically limited.

[0048] The second driving mechanism 160 comprises a third driving member 161, a third lead screw 162 and a third nut 163. The third driving member 161 is connected with the third lead screw 162, the third nut 163 is sleeved outside the third lead screw 162 and threadedly matched with the third lead screw 162, the third nut 163 is connected with the bearing table 170, and the axial direction of the third lead screw 162 is the third direction. Specifically, the third driving member 161 is used for driving the third lead screw 162 to rotate, so as to drive the third nut 163 to displace along the axial direction of the third lead screw 162, thereby driving the bearing table 170 to move in the third direction.

[0049] Further, the rack 110 is provided with a third guide rail 112, and the bearing table 170 is provided with a third sliding block 171. The third sliding block 171 is slidably matched with the third guide rail 112, the extending direction of the third guide rail 112 is the third direction, the third sliding block 171 can slide relative to the third guide rail 112, and the third guide rail 112 can guide and limit the third sliding block 171, so as to ensure the stability of the sliding of the bearing table 170 relative to the rack 110.

[0050] Please continue to refer to Figure 2 The push-pull mechanism 140 comprises a fourth driving member 141, a fourth screw rod 142 and a fourth nut 143. The fourth driving member 141 is connected with the fourth screw rod 142, the fourth nut 143 is sleeved outside the fourth screw rod 142 and is in threaded cooperation with the fourth screw rod 142, the fourth nut 143 is connected with the push rod 152, and the axial direction of the fourth screw rod 142 is the same as the extending direction of the needle tube 151, that is, the vertical direction. Specifically, the fourth driving member 141 is used to drive the fourth screw rod 142 to rotate, so that the fourth nut 143 is displaced along the axial direction of the fourth screw rod 142, thereby driving the push rod 152 to move along the vertical direction.

[0051] Further, the mounting frame 130 is provided with a fourth guide rail 132, the fourth nut 143 is provided with a fourth sliding block 144, the fourth sliding block 144 is in sliding cooperation with the fourth guide rail 132, the extending direction of the fourth guide rail 132 is the vertical direction, the fourth sliding block 144 can slide relative to the fourth guide rail 132, and the fourth guide rail 132 can guide and limit the fourth sliding block 144, so as to ensure the stability of the sliding of the fourth nut 143 relative to the mounting frame 130.

[0052] In the embodiment, the first driving member 1211, the second driving member 1231, the third driving member 161 and the fourth driving member 141 are all driving motors, but are not limited thereto. In other embodiments, the first driving member 1211, the second driving member 1231, the third driving member 161 and the fourth driving member 141 can all be pneumatic motors or hydraulic motors, and the type of the first driving member 1211, the second driving member 1231, the third driving member 161 and the fourth driving member 141 is not limited in particular.

[0053] The drop driving device 100 provided by the embodiment of the utility model, the first driving mechanism 120 is installed in the rack 110 and is connected with the mounting frame 130, the drop injector 150 comprises a needle tube 151 and a push rod 152, the push rod 152 is slidably arranged in the needle tube 151, the needle tube 151 is connected with the mounting frame 130, the push-pull mechanism 140 is installed in the mounting frame 130 and is connected with the push rod 152, the first driving mechanism 120 is used to drive the push-pull mechanism 140 and the drop injector 150 to move synchronously through the mounting frame 130, and the push-pull mechanism 140 is used to drive the push rod 152 to slide relative to the needle tube 151. Compared with the prior art, the drop driving device 100 provided by the utility model can realize automatic drop operation, improve drop efficiency, improve drop quantity precision, save labor cost and time cost, ensure nucleic acid detection efficiency and nucleic acid detection effect, and make the digital PCR detection equipment 10 have high detection efficiency and good detection effect.

[0054] The preferred embodiments of the present application have been described above with the preferred embodiments, but are not intended to limit the present application. For those skilled in the art, the present application can have various changes and variations. Any modification, equivalent replacement, improvement, etc. made within the spirit and principle of the present application shall be included in the protection scope of the present application.

Claims

1. A droplet drive apparatus characterized by comprising: The device comprises a rack, a first driving mechanism, a mounting rack, a push-pull mechanism and a droplet injector, the first driving mechanism is mounted on the rack and connected with the mounting rack, the droplet injector comprises a needle tube and a push rod, the push rod is slidably arranged in the needle tube, the needle tube is connected with the mounting rack, the push-pull mechanism is mounted on the mounting rack and connected with the push rod, the first driving mechanism is used to drive the push-pull mechanism and the droplet injector to move synchronously through the mounting rack, and the push-pull mechanism is used to drive the push rod to slide relative to the needle tube.

2. The drop drive device according to claim 1, characterized by The first driving mechanism comprises a first driving assembly, a translation rack and a second driving assembly, the first driving assembly is mounted on the rack and connected with the translation rack, the first driving assembly is used to drive the translation rack to move in a first direction, the second driving assembly is mounted on the translation rack and connected with the mounting rack, and the second driving assembly is used to drive the mounting rack to move up and down in a second direction, the first direction is perpendicular to the second direction.

3. The drop drive device of claim 2, wherein The first driving assembly comprises a first driving member, a first screw rod and a first nut, the first driving member is connected with the first screw rod, the first nut is sleeved outside the first screw rod and threadedly matched with the first screw rod, the first nut is connected with the translation rack, and the axial direction of the first screw rod is the first direction.

4. The drop drive device of claim 2, wherein The rack is provided with a first guide rail, the translation rack is provided with a first sliding block, the first sliding block is slidably matched with the first guide rail, and the extension direction of the first guide rail is the first direction.

5. The drop drive device of claim 2, wherein The second driving assembly comprises a second driving member, a second screw rod and a second nut, the second driving member is connected with the second screw rod, the second nut is sleeved outside the second screw rod and threadedly matched with the second screw rod, the second nut is connected with the mounting rack, and the axial direction of the second screw rod is the second direction.

6. The drop drive device of claim 2, wherein The droplet driving device further comprises a second driving mechanism and a bearing table, the second driving mechanism is mounted on the rack and connected with the bearing table, the second driving mechanism is used to drive the bearing table to move in a third direction, and the first direction, the second direction and the third direction are perpendicular to each other.

7. The drop drive device of claim 6, wherein The second driving mechanism comprises a third driving member, a third screw rod and a third nut, the third driving member is connected with the third screw rod, the third nut is sleeved outside the third screw rod and threadedly matched with the third screw rod, the third nut is connected with the bearing table, and the axial direction of the third screw rod is the third direction.

8. The drop drive device of claim 1, wherein The push-pull mechanism comprises a fourth driving member, a fourth screw rod and a fourth nut, the fourth driving member is connected with the fourth screw rod, the fourth nut is sleeved outside the fourth screw rod and threadedly matched with the fourth screw rod, the fourth nut is connected with the push rod, and the axial direction of the fourth screw rod is the same as the extension direction of the needle tube.

9. The drop drive device of claim 1, wherein The needle tube and the push rod are arranged in a vertical direction, and the push-pull mechanism is connected to the top of the push rod.

10. A digital PCR detection device, characterized by, The device comprises the droplet driving device according to any one of claims 1-9.

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