Movable optical fiber fixing device for optical genetic experiment

Abstract

The utility model relates to the technical field of optical fiber fixing, and provides a movable optical fiber fixing device for an optogenetic experiment, which comprises a round steel bar, a slide rail and a frame body, and solves the problem that an optical fiber is easy to fall to the ground or the redundancy is too long by filling the blank that no fixing device is provided for the optical fiber in an animal optogenetic behavior experiment. By flexibly adjusting the position of the optical fiber, interference of the optical fiber on mouse behaviors is reduced to the greatest extent, and the reliability of experimental results is ensured. And the two hands and the time of experimenters are liberated, so that the optogenetic behavior experiment of more mice can be carried out at the same time in the same time, and the experiment efficiency is remarkably improved. Through the cooperation of the sliding rails and the wheels, the stability of the optical fiber fixing device in the moving process is ensured, and the phenomenon that a fixed part is loosened or falls off is avoided. The device is simple in structure and convenient to operate, the tightness of the clamping device is adjusted by pushing the screw rod, and adjustment can be easily carried out according to different optical fiber diameters.

Description

TECHNICAL FIELD

[0001] The utility model relates to the technical field of optical fiber fixing, especially relates to a movable optical fiber fixing device for optical genetic experiment. BACKGROUND

[0002] With the rapid development of brain science, scientists have deepened their understanding of neural nuclei and their pathways. Traditional neural pathway verification methods mainly rely on microscopic molecular immunology research, however, this method can only provide static structural information and cannot directly reflect the functional state of neural pathways in living organisms.

[0003] In order to more comprehensively verify the function of a specific neural pathway, researchers have gradually turned their attention to behavioral verification at the behavioral level, that is, by interfering with a specific pathway, observing whether the animal exhibits the expected behavioral response. In recent years, the rise of optogenetics has provided a new means for this research. Optogenetics involves precisely introducing exogenous light-sensitive protein genes into living cells, allowing these cells to express light-sensitive proteins. When light of a specific wavelength shines on these cells, the channels of the light-sensitive proteins open or close, causing changes in the cell membrane potential. This method allows precise control of specific neurons with minimal damage to cells, and even non-invasive. The emergence of optogenetics not only provides a powerful tool for neuroscience research, but also opens up new directions for animal behavioral experiments. Through optogenetic technology, researchers can precisely manipulate specific neural pathways without damaging the animal and observe the resulting behavioral changes. This method greatly improves the accuracy and repeatability of experiments, allowing scientists to more deeply explore the relationship between neural pathways and behavior.

[0004] Therefore, optogenetic behavioral experiments have emerged and quickly become an important means of neuroscience and behavioral research. Through this technology, researchers can manipulate neural activity in live animals in real time, observe and record the behavioral responses of animals after a specific neural pathway is intervened, thereby providing strong support for revealing the functional mechanisms of neural circuits. In traditional mouse optogenetic behavioral experiments, the fixation and positioning of optical fibers have always been key issues in experimental design and implementation. In previous experiments, optical fibers were usually randomly placed on the walls of the mine or the walls of the elevated plus maze. This method has the following significant problems:

[0005] Optical fibers are prone to falling or being excessively long: without a dedicated fixing device, optical fibers are prone to falling from the walls or being excessively long in the air, causing the optical fibers to drag the mouse's head and limit the mouse's normal movement.

[0006] Low experimental efficiency: due to the unstable position of the optical fiber, the experimenter needs to be near the experimental site at all times to observe whether the length of the optical fiber affects the behavior of the mouse and manually adjust the dragging length and placement position of the optical fiber at any time. This makes it possible to carry out only one optical genetic behavior experiment of a mouse in the same time, which seriously reduces the experimental efficiency.

[0007] Experimental result bias: the dragging effect of the optical fiber will interfere with the natural behavior of the mouse, causing the experimental results to be biased and unable to accurately reflect the real behavior of the mouse under light stimulation. Practical new type content

[0008] The utility model discloses a movable optical fiber fixing device for optical genetic experiment, adopt this device to work to solve above -mentioned problem.

[0009] In order to solve the above technical problem, the utility model provides the following technical scheme: a movable optical fiber fixing device for optical genetic experiment, including round steel strip, slide rail and frame body, the inner side wall of round steel strip is provided with slide rail, the outside of slide rail is provided with fixed mobile subassembly, the periphery of round steel strip bottom is provided with frame body, and the top of frame body is provided with connecting adjustment subassembly.

[0010] Preferably, the fixed mobile subassembly includes a sliding seat provided on the slide rail, a support seat fixed on the sliding seat, support blocks fixed on both sides of the front end of the support seat, clamps provided on both sides between the two support blocks, a push screw threadedly connected in the support block, and the push screw connected with the clamp at one end.

[0011] Preferably, the connecting adjustment subassembly includes a steel strip limiting seat fixed on the top of the frame body, the steel strip limiting seat is clamped on the outer side of the round steel strip, a fixed buckle is provided on the outer side of the steel strip limiting seat, the fixed buckle is threadedly connected with fixed bolts on both sides, and the fixed bolts are screwed on the side wall of the round steel strip at one end.

[0012] Preferably, the bottom of the frame body is provided with a base, a sliding groove is formed in the inside of the base, a sliding block is arranged in the inside of the sliding groove, the top end of the sliding block is fixedly connected with the bottom end of the frame body, an external block is arranged on the top of the base, the external block is threadedly connected with locking bolts on both sides, and the bottom of the locking bolts is tightly abutted with the top end of the base.

[0013] Compared with the prior art, the utility model has the beneficial effects as follows:

[0014] The utility model provides a movable optical fiber fixing device for light genetic experiment, through the blank of no fixing device of optical fiber in animal light genetic behavior experiment is filled up, the problem that optical fiber is easy to fall to the ground or redundant is too long is solved. Through the position of optical fiber is adjusted flexibly, the interference of optical fiber to mouse behavior is reduced to the maximum, ensure the reliability of experimental result. The hands and time of experimental personnel are liberated, so that more mice light genetic behavior experiments can be carried out simultaneously in the same time, and the experimental efficiency is improved significantly. Through the cooperation of slide rail and wheel, the stability of optical fiber fixing device in the moving process is ensured, and the phenomenon that the fixed part is loose or falls off is avoided. The device has simple structure and convenient operation, the tightness of the holder is adjusted by pushing the screw rod, and it is easy to adjust according to different optical fiber diameters, and the device is suitable for various experimental scenes such as mine field experiment and overhead cross experiment, and can meet different experimental requirements. BRIEF DESCRIPTION OF DRAWINGS

[0015] Fig. 1 It is the overall structure schematic diagram of the utility model;

[0016] Fig. 2 It is the local structure split drawing of the utility model;

[0017] Fig. 3 It is the fixed mobile assembly structure schematic diagram of the utility model;

[0018] Fig. 4 It is the connection adjustment assembly structure split drawing of the utility model.

[0019] The reference signs in the drawing are explained: 1, round steel strip; 2, slide rail; 21, slide base; 22, support seat; 23, holder; 24, support block; 25, pushing screw rod; 3, frame body; 31, steel strip limiting seat; 32, fixed buckle; 33, fixed bolt; 34, base; 35, sliding groove; 36, sliding block; 37, external block; 38, locking bolt. DETAILED DESCRIPTION

[0020] The technical scheme in the embodiments of the utility model will be described clearly and completely in combination with the drawings in the embodiments of the utility model, and obviously, the described embodiments are only a part of the embodiments of the utility model, not all the embodiments. Based on the embodiments in the utility model, all other embodiments obtained by the ordinary skill in the art without making creative labor belong to the range of protection of the utility model.

[0021] In order to further understand the contents of the utility model, the utility model is described in detail in combination with the drawings.

[0022] In combination with Figs. 1 to 4The utility model discloses a movable optical fiber fixing device for optical genetic experiment, including round steel strip 1, slide rail 2 and frame body 3, the inner side wall of round steel strip 1 is provided with slide rail 2, the outside of slide rail 2 is provided with fixed mobile subassembly, the periphery of round steel strip 1 bottom is provided with frame body 3, and the top of frame body 3 is provided with connecting adjustment subassembly.

[0023] Fixed mobile subassembly includes the slide seat 21 set up on slide rail 2, and the support seat 22 is fixed on the slide seat 21, and the both sides of the front end of support seat 22 are fixed with support block 24, and the both sides between two support blocks 24 are provided with clamping device 23, and the inside screw of support block 24 is connected with push screw 25, and one end of push screw 25 is connected with clamping device 23.

[0024] Connecting adjustment subassembly includes the steel strip limiting seat 31 fixed on the top of frame body 3, and the steel strip limiting seat 31 is clamped on the outside of round steel strip 1, and the outside of steel strip limiting seat 31 is provided with fixed buckle 32, and the both sides of fixed buckle 32 are screw connected with fixed bolt 33, and one end of fixed bolt 33 is rotated on the side wall of round steel strip 1.

[0025] The bottom of frame body 3 is provided with base 34, the inside of base 34 is provided with sliding slot 35, the inside of sliding slot 35 is provided with sliding block 36, the top of sliding block 36 is fixedly connected with the bottom end of frame body 3, the top of base 34 is provided with external block 37, the both sides of external block 37 are screw connected with locking bolt 38, and the bottom of locking bolt 38 is tightly connected with the top end of base 34.

[0026] Working principle:

[0027] Round steel strip 1 is surrounded by steel strip, and four frame bodies 3 are installed around the mine field experiment or overhead cross experiment, and the steel strip is installed in the inside of the steel strip limiting seat 31 on the top of four frame bodies 3, the fixed buckle 32 is clamped on the outside of the steel strip limiting seat 31, and the round steel strip 1 is fixed on the inside of the steel strip limiting seat 31 by using the fixed bolt 33 and the fixed buckle 32, so that the installation of the round steel strip 1 and the frame body 3 can be completed.

[0028] The position of frame body 3 can be adjusted, the sliding block 36 at the bottom of frame body 3 is slid in the inside of sliding slot 35 by pushing frame body 3, so that the position of frame body 3 can be changed, after moving frame body 3 to different positions on the top of base 34, rotating locking bolt 38 makes the bottom end tightly connected with the top end of base 34, the position of frame body 3 is fixed, so that the adjustment of the position of frame body 3 can be completed, in the mine field experiment or overhead cross experiment, the operator can flexibly adjust the distance between the four frame bodies 3 of the device according to the experimental requirements.

[0029] The inner side of the round steel strip 1 is fixed with a circle of slide rails 2 for the movement of the optical fiber fixing device, the slide rails 2 and slide blocks 21 are integrated, like the rails of concrete sleepers and rails, the whole slide rails 2 can be assembled in sections, and the experimenter can freely assemble according to the size required by the experimental site, so as to realize the slide rails 2 with different diameters;

[0030] The optical fiber is placed between the two clamps 23, the two clamps 23 are pushed close to the optical fiber by rotating the push screw 25, the tightness of the clamps 23 is adjusted, the tightness of the clamps 23 is ensured, the clamps 23, the supporting blocks 24 and the push screw 25 are the fixing part of the optical fiber, the experimental animal pulls the fixing part by moving by itself, the fixing part freely slides on the slide rails 2 by the wheels of the slide blocks 21, so as to ensure that the position of the optical fiber fixing device can be adjusted in real time according to the movement of the animal, wherein the cooperation of the slide rails 2 and the wheels ensures the stability and accuracy of the optical fiber fixing device during the movement.

[0031] It should be noted that, in the present text, the relationship terms such as first and second are only used to distinguish one entity or operation from another entity or operation, and do not necessarily require or imply any such actual relationship or order between the entities or operations. Moreover, the terms "include", "contain" or any other variants thereof are intended to cover non-exclusive inclusion, so that the process, method, article or equipment including a series of elements not only includes those elements, but also includes other elements not explicitly listed or inherent to such process, method, article or equipment.

[0032] Although the embodiments of the present application have been shown and described, it can be understood by those skilled in the art that various changes, modifications, replacements and variations can be made to the embodiments without departing from the principles and spirits of the present application, and the scope of the present application is defined by the appended claims and their equivalents.

Claims

1. A movable optical fiber fixing device for optogenetic experiments, comprising a circular steel bar (1), a sliding rail (2) and a frame (3), characterized in that: The inner side wall of the circular steel strip (1) is provided with a sliding rail (2), the outer part of the sliding rail (2) is provided with a fixing moving assembly, the bottom of the circular steel strip (1) is provided with a frame body (3), and the top end of the frame body (3) is provided with a connecting adjusting assembly.

2. The movable optical fiber fixation device for optogenetic experiments according to claim 1, wherein: The fixing moving assembly comprises a sliding seat (21) arranged on the sliding rail (2), a supporting seat (22) fixed on the sliding seat (21), supporting blocks (24) fixed on the two sides of the front end of the supporting seat (22), clamps (23) arranged on the two sides between the two supporting blocks (24), a pushing screw (25) threadedly connected in the supporting block (24), and one end of the pushing screw (25) connected with the clamp (23).

3. The movable optical fiber fixation device for optogenetic experiments of claim 2, wherein: The connecting adjusting assembly comprises a steel strip limiting seat (31) fixed on the top end of the frame body (3), the steel strip limiting seat (31) is clamped on the outer side of the circular steel strip (1), the outer side of the steel strip limiting seat (31) is provided with a fixing buckle (32), the two sides of the fixing buckle (32) are threadedly connected with fixing bolts (33), and one end of the fixing bolt (33) is screwed on the side wall of the circular steel strip (1).

4. The movable optical fiber fixation device for optogenetic experiments of claim 3, wherein: The bottom of the frame body (3) is provided with a base (34), the inside of the base (34) is provided with a sliding groove (35), the inside of the sliding groove (35) is provided with a sliding block (36), the top end of the sliding block (36) is fixedly connected with the bottom end of the frame body (3), the top of the base (34) is provided with an external block (37), the two sides of the external block (37) are threadedly connected with locking bolts (38), and the bottom of the locking bolt (38) is tightly abutted with the top end of the base (34).

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