Amino acid paper chromatography experimental apparatus

Abstract

The utility model relates to an amino acid paper chromatography experiment appliance, which belongs to the technical field of amino acid chromatography experiments and comprises a chromatography sample application scale, a chromatography developing vessel, a chromatography liquid small groove, a cylindrical filter paper core and circular chromatography filter paper. And the outer side is hollowed out for drawing the front edge of chromatographic liquid. According to the utility model, the design of the chromatography sample application scale helps an operator to draw a starting line of a sample application original point, a finishing line of chromatography expansion and measure the distance between the sample application original point and an amino acid color spot, so that the adverse effects of inclination and tilting of filter paper on an experiment in the traditional chromatography process are avoided from the source in the horizontal chromatography direction from the center to the circumference; the experiment efficiency and success rate are greatly improved, the chromatography vessel is smaller in size compared with a traditional chromatography device, the use amount of chromatography liquid is effectively reduced through the design of the chromatography liquid small groove, and the device is more environmentally friendly during use.

Description

Technical Field

[0001] This utility model relates to the field of amino acid chromatography experimental technology, and in particular to an amino acid paper chromatography experimental apparatus. Background Technology

[0002] Paper chromatography is a partition chromatography method using filter paper as the support. When a water-miscible organic solvent is used as the developing solvent, the numerous hydroxyl groups distributed on the filter paper fibers form hydrogen bonds with water molecules, strongly binding them and serving as the stationary phase; the organic solvent has a weak affinity for the filter paper fibers and serves as the mobile phase. Paper chromatography can be used to separate components in amino acid mixtures. Amino acids of different polarities exhibit different migration rates (Rf) in paper chromatography; the Rf value of more polar amino acids is lower than that of less polar amino acids, thus enabling the separation and identification of different amino acids.

[0003] The existing amino acid paper chromatography experimental operation technique has the following defects: 1. In order to ensure that the origins of each sample spot are on the same starting line and come into contact with the developing liquid at the same time, the line connecting each origin (starting line) needs to be horizontal with the edge of the filter paper. This requires the operator to draw a horizontal starting line with a ruler before spotting the sample. However, in actual operation, it is difficult to guarantee success on the first try, and repeated drawing of the line affects the subsequent chromatographic development. 2. The bottom-up chromatographic direction requires the filter paper after spotting to be rolled into a tube and placed vertically into a petri dish containing the developing solution. The tilting or falling of the tube during the chromatography process directly affects the success rate of chromatography. 3. In traditional methods, the developing solution needs to be soaked in the lower edge of the tube for 8-10 mm, so the amount of organic solvent used is relatively large. During the process of taking the filter paper out and putting it in, a large amount of organic solvent vapor overflows outside the chromatography rod, polluting the environment. To address this, an amino acid paper chromatography experimental apparatus is proposed. Compared with traditional chromatography devices, it is more compact in size. The small tank design reduces the amount of chromatography solution used, making it more environmentally friendly. The horizontal chromatographic direction from the center to the circumference and the design of the chromatographic spotting scale effectively simplify the experimental operation and improve experimental efficiency and success rate. Utility Model Content

[0004] This invention provides an amino acid paper chromatography apparatus that solves the problems mentioned in the background.

[0005] The present invention provides the following solution to the above-mentioned technical problems: an amino acid paper chromatography apparatus, comprising a chromatography spotting scale, a chromatography developing dish, a chromatography solvent trough, a cylindrical filter paper core, and a circular chromatography filter paper. The chromatography spotting scale has two concentric circular cutouts. The inner cutout is used to draw the origin of the chromatography sample loading, and the outer cutout is used to draw the front edge of the chromatography solvent. Six graduation lines originating from the center of the two cutouts are provided between them. The chromatography solvent trough is placed in the chromatography developing dish. A vertical column is fixed at the center of the chromatography solvent trough. The vertical column can help fix the cylindrical filter paper core placed in the center of the circular chromatography filter paper. The chromatography spotting scale is placed on top of the circular chromatography filter paper. The cylindrical filter paper core is hollow, 1 cm high, and 6 mm in diameter, and is made of 1 cm × 2.5 cm filter paper strips rolled up. The cylindrical filter paper core is vertically inserted into the circular chromatography filter paper. The circular chromatography filter paper has a 6 mm diameter hole corresponding to the cylindrical filter paper core.

[0006] The chromatography developing dish includes a lid and a bottom.

[0007] Based on the above technical solution, the present invention can be further improved as follows.

[0008] Furthermore, the chromatography spotting scale is a transparent plastic disc with a diameter of 15cm and a thickness of 2.5mm. A cross is perpendicularly intersecting the center of the scale, and there are two semi-circular cutouts with a radius of 1cm from the center, which are used to draw the starting line of the spotting origin.

[0009] Furthermore, the chromatography spotting scale has two semi-circular cutouts with a radius of 6 cm from the center, which are used to draw the endpoint line of the chromatography front, which specifies the chromatography development.

[0010] Furthermore, six scale lines and serial numbers are drawn between the two hollowed-out areas of the chromatography spotting scale, originating from the center of the circle, to assist in measuring the distance between the spotting origin and the amino acid spot. The serial numbers 1 to 6 correspond to a total length of 1 cm to 6 cm, respectively, and the last centimeter of each scale line is subdivided into 1 mm increments.

[0011] Furthermore, the chromatography tank is made of polypropylene, with an inner diameter of 2.7cm, an outer diameter of 3cm, a height of 0.7cm, and a bottom wall thickness of 0.15cm. The vertical solid column at the center of the chromatography tank has a diameter of 3mm and a height of 1cm.

[0012] Furthermore, the chromatography developing dish is made of glass, the dish lid has an inner diameter of 12.4 cm, an outer diameter of 13 cm, a height of 1 cm, and a top wall thickness of 0.2 cm, and the dish bottom has an inner diameter of 12.4 cm, an outer diameter of 13 cm, a height of 1 cm, and a bottom wall thickness of 0.2 cm.

[0013] This invention provides an amino acid paper chromatography apparatus, which has the following advantages:

[0014] 1. The design of the chromatography spotting scale helps operators draw the spotting origin "starting line", the chromatography development "finish line", and measure the distance between the spotting origin and the amino acid spot;

[0015] 2. The horizontal chromatography direction from the center to the circumference avoids the adverse effects of filter paper tilting or leaning on the experiment in the traditional chromatography process from the source, greatly improving experimental efficiency and success rate;

[0016] 3. Compared with traditional chromatography apparatus, the chromatography dish is more compact in size, and the design of the small chromatography solvent tank effectively reduces the amount of chromatography solvent used (only 4-5 mL of chromatography developing solvent is needed), making it more environmentally friendly to use.

[0017] The above description is merely an overview of the technical solution of this utility model. In order to better understand the technical means of this utility model and to implement it according to the contents of the specification, the preferred embodiments of this utility model are described in detail below with reference to the accompanying drawings. The specific implementation methods of this utility model are given in detail in the following embodiments and their accompanying drawings. Attached Figure Description

[0018] The accompanying drawings, which are included to provide a further understanding of the present invention and form part of this application, illustrate exemplary embodiments of the present invention and, together with the description thereof, serve to explain the present invention and do not constitute an undue limitation thereof. In the drawings:

[0019] Figure 1 This is a schematic diagram of the structure of an amino acid paper chromatography experimental apparatus provided in one embodiment of the present invention;

[0020] Figure 2 This is a schematic diagram of the structure of a chromatographic spotting scale in an amino acid paper chromatography experimental apparatus according to an embodiment of the present invention;

[0021] Figure 3 A three-dimensional rendering of the assembled state of a chromatography developing dish in an amino acid paper chromatography experimental apparatus provided in an embodiment of this utility model;

[0022] Figure 4 A schematic diagram showing the disassembly of a chromatography developing dish in an amino acid paper chromatography experimental apparatus according to an embodiment of this utility model;

[0023] Figure 5 A schematic diagram showing the disassembly of the chromatographic spotting scale and circular chromatographic filter paper in an amino acid paper chromatography experimental apparatus provided in an embodiment of this utility model;

[0024] Figure 6 This is a schematic diagram illustrating the assembly of a chromatographic spotting scale and a circular chromatographic filter paper in an amino acid paper chromatography experimental apparatus according to an embodiment of the present invention.

[0025] Figure 7 This is a schematic diagram showing the disassembled structure of an amino acid paper chromatography apparatus according to an embodiment of the present invention.

[0026] The attached diagram lists the components represented by each number as follows:

[0027] 1. Chromatography spotting scale; 2. Chromatography developing dish; 201. Dish lid; 202. Dish bottom; 3. Chromatography solvent trough; 4. Cylindrical filter paper core; 5. Circular chromatography filter paper. Detailed Implementation

[0028] The following is in conjunction with the appendix Figure 1-7 The principles and features of this utility model are described below. The examples given are for illustrative purposes only and are not intended to limit the scope of this utility model. The utility model is described more specifically in the following paragraphs by way of example with reference to the accompanying drawings. The advantages and features of this utility model will become clearer from the following description and claims. It should be noted that the drawings are all in a very simplified form and use non-precise proportions, and are only used to facilitate and clarify the illustration of the embodiments of this utility model.

[0029] It should be noted that when a component is described as "fixed to" another component, it can be directly on the other component or may have a component in between. When a component is considered "connected to" another component, it can be directly connected to the other component or may have a component in between. When a component is considered "set on" another component, it can be directly set on the other component or may have a component in between. The terms "vertical," "horizontal," "left," "right," and similar expressions used in this document are for illustrative purposes only.

[0030] Unless otherwise defined, all technical and scientific terms used herein have the same meaning as commonly understood by one of ordinary skill in the art to which this invention pertains. The terminology used herein is for the purpose of describing particular embodiments only and is not intended to be limiting of the invention. The term "and / or" as used herein includes any and all combinations of one or more of the associated listed items.

[0031] like Figure 1-7As shown, an amino acid paper chromatography apparatus includes a chromatography spotting scale 1, a chromatography developing dish 2, a chromatography solvent trough 3, a cylindrical filter paper core 4, and a circular chromatography filter paper 5. The chromatography spotting scale 1 has two concentric circular cutouts; the inner cutout is used to mark the origin of the chromatography sample loading, and the outer cutout is used to mark the front of the chromatography solvent. Six graduation lines originating from the center of the two cutouts are arranged between them. The chromatography solvent trough 3 is placed in the chromatography developing dish 2, and a vertical [unclear text - possibly a reference to a vertical axis or groove] is fixed at the center of the chromatography solvent trough 3. The small column, vertical column can help fix the cylindrical filter paper core 4 placed in the center of the circular chromatography filter paper 5. The chromatography spotting scale 1 is placed on the top of the circular chromatography filter paper 5. The cylindrical filter paper core 4 is hollow, 1cm high and 6mm in diameter, and is made of 1cm×2.5cm filter paper strips rolled up. The cylindrical filter paper core 4 is vertically inserted into the circular chromatography filter paper 5. The circular chromatography filter paper 5 has a 6mm diameter hole corresponding to the cylindrical filter paper core 4. The chromatography developing dish 2 includes a dish lid 201 and a dish bottom 202.

[0032] Preferably, the chromatography spotting scale 1 is a transparent plastic disc with a diameter of 15cm and a thickness of 2.5mm. There is a cross perpendicular to the center of the scale, and two semi-circular cutouts with a radius of 1cm from the center are used to draw the starting line of the spotting origin.

[0033] Preferably, the chromatography spotting scale 1 has two semi-circular arc-shaped cutouts with a radius of 6 cm from the center, which are used to draw the endpoint line of the chromatography front that specifies the chromatography development.

[0034] Preferably, six scale lines and serial numbers are drawn between the two hollowed-out areas of the chromatography spotting scale 1, starting from the center of the circle, to assist in measuring the distance between the spotting origin and the amino acid spot. The serial numbers 1 to 6 correspond to a total length of 1 cm to 6 cm, respectively, and the last centimeter of each scale line is subdivided into 1 mm scales.

[0035] Preferably, the chromatography tank 3 is made of polypropylene, with an inner diameter of 2.7cm, an outer diameter of 3cm, a height of 0.7cm, a bottom wall thickness of 0.15cm, and a vertical solid column in the center of the chromatography tank 3 with a diameter of 3mm and a height of 1cm.

[0036] Preferably, the chromatography developing dish 2 is made of glass, the dish lid 201 has an inner diameter of 12.4cm, an outer diameter of 13cm, a height of 1cm, and a top wall thickness of 0.2cm, and the dish bottom 202 has an inner diameter of 12.4cm, an outer diameter of 13cm, a height of 1cm, and a bottom wall thickness of 0.2cm.

[0037] The specific working principle and usage method of this utility model are as follows:

[0038] 1. Filter paper preparation: Use a hole punch to make a 6mm diameter hole in the center of a 160mm diameter circular chromatography filter paper 5. Attach the chromatography spotting scale 1 above the circular chromatography filter paper 5 and adjust its position so that the center cross of the chromatography spotting scale 1 coincides with the center of the small hole in the circular chromatography filter paper 5 (as per the instruction manual). Figure 6 (As shown), use a pencil to draw the starting line for the sample origin along the "semi-circular" cutout on the inner side of the chromatography sample spotting scale 1. Define the sample origin on the starting line and mark it with a pencil. Use a pencil to draw the finish line for the chromatography development along the "semi-circular" cutout on the outer side of the chromatography sample spotting scale 1 (as shown in the instruction manual). Figure 2 (as shown);

[0039] 2. Spotting: Take 1-2 μL of amino acid solution and spot it at the spotting origin marked with a pencil. After each spotting point dries, continue spotting at the same point 15-20 times.

[0040] 3. Assembly of chromatography filter paper: Cut a 1cm × 2.5cm strip of filter paper, roll it into a cylindrical filter paper core, and then insert it into the center hole of the circular chromatography filter paper (as per the instruction manual). Figure 7 (As shown).

[0041] 4. Preparation of developing phase: Pour 4-5 mL of developing buffer into the chromatography tank 3, then assemble the chromatography apparatus (as per the instruction manual). Figure 3 As shown), at this time, the lower edge of the cylindrical filter paper core 4 is immersed in the chromatography developing solution, and then the circular chromatography filter paper 5 is horizontally developed from the center to the outer circumference. The edge of the circular chromatography filter paper 5 is sandwiched between the plate lid 201 and the plate bottom 202.

[0042] 5. Development process: Observe the position of the development front of the chromatography solvent through the glass plate lid 201. When the development front coincides with the "end line" of chromatography development, remove the circular chromatography filter paper 5 and pull out the cylindrical filter paper core 4 to end the development process.

[0043] 6. Color Development and Rf Calculation: After drying the circular chromatography filter paper 5 with a hair dryer, spray ninhydrin solution evenly onto the circular chromatography filter paper 5 using a spray bottle. Heat and dry it with a hair dryer. At this time, purple-blue spots will gradually appear on the filter paper. Align the center of the crosshairs of the chromatography spotting scale 1 with the center of the circular chromatography filter paper 5, fix the center, and rotate the chromatography spotting scale 1 to measure the distance between the spotting origin and the amino acid spot using the scale lines on it. Calculate the Rf value of each spot (distance from origin to amino acid spot / distance from origin to the front of the developing solvent).

[0044] 7. End: Collect the developing solution into a dedicated recovery container, rinse the equipment with clean water, dry it, and store it away.

[0045] The above description is merely a preferred embodiment of this utility model and is not intended to limit the utility model in any way. Those skilled in the art can readily implement this utility model based on the accompanying drawings and the above description. However, any modifications, alterations, or variations made by those skilled in the art without departing from the scope of the utility model's technical solution, utilizing the disclosed technical content, are equivalent embodiments of this utility model. Furthermore, any equivalent changes, alterations, or variations made to the above embodiments based on the essential technology of this utility model are still within the protection scope of this utility model's technical solution.

Claims

1. An amino acid paper chromatography experimental apparatus, comprising a chromatography spotting scale (1), a chromatography developing dish (2), a chromatography liquid trough (3), a cylindrical filter paper core (4), and a circular chromatography filter paper (5), characterized in that: The chromatography spotting scale (1) is provided with two concentric circle hollows, and six scale lines are drawn from the center of the circle, the chromatography liquid groove (3) is placed in the chromatography development dish (2), the chromatography liquid groove (3) is fixed with a vertical small column in the center, the chromatography spotting scale (1) is placed on the top of the circular chromatography filter paper (5), the cylindrical filter paper core (4) is hollow, 1cm high and 6mm in diameter, which is rolled by a 1cm×2.5cm filter paper strip, the cylindrical filter paper core (4) is vertically inserted into the circular chromatography filter paper (5), and the circular chromatography filter paper (5) is provided with a 6mm diameter hole corresponding to the cylindrical filter paper core (4). The chromatography development dish (2) comprises a dish cover (201) and a dish bottom (202).

2. The amino acid paper chromatography laboratory kit according to claim 1, wherein The chromatography spotting scale (1) is a transparent plastic circle with a diameter of 15cm and a thickness of 2.5mm, and a cross is vertically crossed in the center of the circle, and two semicircle arc hollows are provided at a radius of 1cm from the center of the circle.

3. The amino acid paper chromatography laboratory kit according to claim 1, wherein The chromatography spotting scale (1) is provided with two semicircle arc hollows at a radius of 6cm from the center of the circle.

4. The amino acid paper chromatography laboratory kit according to claim 1, wherein Six scale lines and serial numbers are drawn between the two hollows of the chromatography spotting scale (1), and the serial numbers 1-6 correspond to the total length of 1cm-6cm, and the last centimeter of a single scale line is subdivided to 1mm.

5. The amino acid paper chromatography laboratory kit of claim 1 wherein, The chromatography liquid groove (3) is made of polypropylene, the inner diameter of the chromatography liquid groove (3) is 2.7cm, the outer diameter is 3cm, the height is 0.7cm, the bottom wall thickness is 0.15cm, the vertical solid small column in the center of the chromatography liquid groove (3) is 3mm in diameter and 1cm in height.

6. The amino acid paper chromatography laboratory kit of claim 1 wherein, The chromatography development dish (2) is made of glass, the inner diameter of the dish cover (201) is 12.4cm, the outer diameter is 13cm, the height is 1cm, and the top wall thickness is 0.2cm, the inner diameter of the dish bottom (202) is 12.4cm, the outer diameter is 13cm, the height is 1cm, and the bottom wall thickness is 0.2cm.

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