Embryo cryopreservation device

Abstract

The utility model belongs to the technical field of embryo preservation, and particularly relates to an embryo cryopreservation device which comprises a freezing box, and preservation devices are arranged at the bottom and inside the freezing box. According to the embryo cryopreservation device, by arranging the damping mechanism, the freezing box pushes a rotating plate to drive a connecting block to extrude a buffer spring and a damping rod, so that vibration from the ground is absorbed and buffered, and the freezing box is prevented from generating excessive vibration; the problems that in the background technology, vibration from the ground cannot be effectively absorbed and buffered in the using process, organelles, cell membranes and other structures in embryos can be damaged under vibration impact, and storage of the embryos is affected are solved. The lower supporting rods are rotated to be in threaded connection with the upper supporting rods, so that the lower supporting rods move up and down along the upper supporting rods, the heights of the four corners of the bottom plate are adjusted, and the freezer is kept horizontal.

Description

Technical Field

[0001] This utility model relates to the field of embryo preservation technology, specifically to an embryo cryopreservation device. Background Technology

[0002] Frozen embryos are the only mature method in the world for preserving fertility. This technique involves placing the removed embryos into straws, pre-cooling them to allow them to cool down, and then placing the straws containing the embryos in a liquid nitrogen environment at -196°C for long-term preservation.

[0003] Existing embryo cryopreservation devices do not have a pre-cooling structure. The straw containing the embryo is placed directly into liquid nitrogen. The large temperature difference between the inside and outside of the liquid nitrogen tank can easily cause the embryo to die, reducing the survival rate of cryopreserved embryos.

[0004] As disclosed in CN222054404U, an embryo cryopreservation device involves placing a straw containing an embryo into a storage cylinder through an inlet and a connection hole. A pre-cooling component pre-cools the straw and embryo inside the storage cylinder. After pre-cooling, a drive component drives a sliding plate to slide down, allowing the storage cylinder and straw to pass through a through hole into the freezing zone. Liquid nitrogen in the freezing zone is used to cryopreserve the embryo inside the straw. This invention pre-cools the straw containing the embryo before placing it into liquid nitrogen for cryopreservation, avoiding embryo death due to excessive temperature differences between the inside and outside of the liquid nitrogen tank, and ensuring the survival rate of cryopreserved embryos.

[0005] However, the device cannot effectively absorb and buffer vibrations from the ground during use, which can damage the organelles, cell membranes and other structures inside the embryo under the impact of vibration, thus affecting the preservation of the embryo.

[0006] Therefore, we urgently need to provide an embryo cryopreservation device. Utility Model Content

[0007] The purpose of this invention is to provide an embryo cryopreservation device to solve the problem mentioned in the background art that the device cannot effectively absorb and buffer vibrations from the ground during use, which can damage the organelles, cell membranes and other structures inside the embryo under vibration impact, thus affecting the preservation of the embryo.

[0008] To achieve the above objectives, the present invention provides the following technical solution: an embryo cryopreservation device, comprising a cryobox, wherein a preservation device is provided at the bottom and inside of the cryobox, and the preservation device includes a shock-absorbing mechanism, a freezing mechanism and an adjusting mechanism.

[0009] The shock absorption mechanism is located at the bottom of the freezer, the freezing mechanism is located inside the freezer, and the adjustment mechanism is located at the bottom of the shock absorption mechanism.

[0010] The shock absorption mechanism includes telescopic rods, a base plate, a mounting plate, a buffer spring, a damping rod, a connecting block, a rotating plate, and a connecting frame. The telescopic rods are fixedly installed around the bottom of the freezer. The base plate is fixedly installed at the bottom of the four telescopic rods. The mounting plate is fixedly installed on the top left and right sides of the base plate. The buffer spring is fixedly installed on the adjacent side of the two mounting plates. The damping rod is installed inside the buffer spring. The connecting block is fixedly installed at the end of the buffer spring away from the mounting plate. One end of the rotating plate is rotatably connected to the outside of the connecting block. The connecting frame is fixedly installed at the bottom of the freezer.

[0011] Preferably, the end of the rotating plate away from the connecting block is rotatably connected to the connecting frame. When the ground vibrates, the rotating plate is pushed by the freezer to cause the connecting block to compress the buffer spring and damping rod, thereby reducing the vibration of the freezer.

[0012] Preferably, the freezing mechanism includes a partition, a fixing frame, a placement plate, a support frame, a handle, a pump body, and a connecting pipe. The partition is fixedly installed inside the freezer, the fixing frame is fixedly installed on the left side inside the freezer, the placement plate is placed inside the freezer, the support frame is fixedly installed on the front and rear sides of the top of the placement plate, the handle is fixedly installed on the top of the support frame, the pump body is fixedly installed on the back of the freezer, and the connecting pipe is connected to the output end of the pump body.

[0013] Preferably, the freezer is equipped with a top cover, and the partition divides the freezer into two cavities. Liquid nitrogen is stored in the right cavity. By activating the pump, liquid nitrogen is introduced from the right cavity into the left cavity through a connecting pipe to freeze the embryos.

[0014] Preferably, the placement plate has a placement hole inside, which facilitates the placement of the wheat tube containing the embryo into the placement plate.

[0015] Preferably, the adjustment mechanism includes an upper support rod, a lower support rod, and a base. The upper support rod is fixedly installed around the bottom of the base plate, the lower support rod is threadedly connected to the lower end of the upper support rod, and the base is fixedly installed at the bottom of the lower support rod.

[0016] Preferably, the upper support rod has an internal thread and the lower support rod has an external thread. When the ground is uneven and the base plate tilts, the lower support rod is rotated to connect with the upper support rod, allowing the lower support rod to move up and down along the upper support rod. This adjusts the height of the four corners of the base plate, keeping the freezer level and preventing uneven liquid nitrogen levels inside the freezer from causing uneven cooling of the wheat tubes and affecting the embryo survival rate.

[0017] Compared with the prior art, the beneficial effects of this utility model are:

[0018] 1. This embryo cryopreservation device, by setting up a shock absorption mechanism, uses a freezing chamber to push a rotating plate, which in turn drives a connecting block to compress a buffer spring and a damping rod, thereby absorbing and buffering vibrations from the ground and preventing excessive vibrations from occurring in the freezing chamber. This solves the problem in the background technology that the device cannot effectively absorb and buffer vibrations from the ground during use, which can damage the organelles, cell membranes, and other structures inside the embryo under vibration impact, thus affecting the preservation of the embryo.

[0019] 2. This embryo cryopreservation device, through the setting of an adjustment mechanism, when the base plate tilts due to uneven ground, can adjust the height of the four corners of the base plate by rotating the threaded connection between the lower support rod and the upper support rod, so that the lower support rod can move up and down along the upper support rod, thereby keeping the cryo-box level. Attached Figure Description

[0020] Figure 1 This is a schematic diagram of the overall structure of this utility model;

[0021] Figure 2 This is a schematic diagram of the internal structure of the freezer of this utility model;

[0022] Figure 3 This is an exploded view of the connection between the fixing frame and the placement plate of this utility model;

[0023] Figure 4 This is a schematic diagram of the shock absorption mechanism of this utility model.

[0024] In the diagram: 1. Freezer; 201. Telescopic rod; 202. Base plate; 203. Mounting plate; 204. Buffer spring; 205. Damping rod; 206. Connecting block; 207. Rotating plate; 208. Connecting frame; 301. Partition; 302. Fixing frame; 303. Placement plate; 304. Support frame; 305. Handle; 306. Pump body; 307. Connecting pipe; 401. Upper support rod; 402. Lower support rod; 403. Base. Detailed Implementation

[0025] The technical solutions of the present utility model will be clearly and completely described below with reference to the accompanying drawings of the embodiments. Obviously, the described embodiments are only some embodiments of the present utility model, and not all embodiments. Based on the embodiments of the present utility model, all other embodiments obtained by those of ordinary skill in the art without creative effort are within the protection scope of the present utility model.

[0026] Example 1:

[0027] Current technologies are unable to effectively absorb and buffer ground vibrations during use, which can damage organelles, cell membranes, and other structures within the embryo, impacting embryo preservation. Please refer to [link to relevant documentation]. Figures 1-4 The embodiment provides an embryo cryopreservation device capable of absorbing and buffering vibrations from the ground, preventing excessive vibration in the cryopreservation chamber. The embryo cryopreservation device includes a cryopreservation chamber 1, with a preservation device located at the bottom and inside the chamber 1. The preservation device includes a shock-absorbing mechanism, a freezing mechanism, and an adjustment mechanism.

[0028] The shock absorption mechanism is located at the bottom of the freezer 1, the freezing mechanism is located inside the freezer 1, and the adjustment mechanism is located at the bottom of the shock absorption mechanism.

[0029] The shock absorption mechanism includes telescopic rods 201, a base plate 202, a mounting plate 203, buffer springs 204, damping rods 205, connecting blocks 206, a rotating plate 207, and a connecting frame 208. The telescopic rods 201 are fixedly installed around the bottom of the freezer 1. The base plate 202 is fixedly installed at the bottom of the four telescopic rods 201. The mounting plates 203 are fixedly installed on the top left and right sides of the base plate 202. The buffer springs 204 are fixedly installed on the adjacent side of the two mounting plates 203. The damping rods 205 are installed on the buffer springs. Inside 204, the connecting block 206 is fixedly installed on the end of the buffer spring 204 away from the mounting plate 203. One end of the rotating plate 207 is rotatably connected to the outside of the connecting block 206. The end of the rotating plate 207 away from the connecting block 206 is rotatably connected to the connecting frame 208. When the ground vibrates, the rotating plate 207 is pushed by the freezer 1 to drive the connecting block 206 to squeeze the buffer spring 204 and the damping rod 205, thereby reducing the vibration of the freezer 1. The connecting frame 208 is fixedly installed at the bottom of the freezer 1.

[0030] To facilitate the placement of the straw containing the embryos into the freezing chamber 1 for embryo freezing, this device is also equipped with a freezing mechanism. The freezing mechanism includes a partition 301, a fixing frame 302, a placement plate 303, a support frame 304, a handle 305, a pump body 306, and a connecting pipe 307. The partition 301 is fixedly installed inside the freezing chamber 1, and a top cover is installed on the top of the freezing chamber 1. The partition 301 divides the freezing chamber 1 into two cavities, with liquid nitrogen stored in the right cavity. By activating the pump body 306, liquid nitrogen is pumped from the right cavity through the connecting pipe... 307 is inserted into the left cavity to freeze the embryo. The fixing frame 302 is fixedly installed inside the left side of the freezer 1. The placement plate 303 is set inside the freezer 1. The placement plate 303 has a placement hole inside, through which the straw for storing the embryo can be easily placed into the placement plate 303. The support frame 304 is fixedly installed on the front and rear sides of the top of the placement plate 303. The handle 305 is fixedly installed on the top of the support frame 304. The pump body 306 is fixedly installed on the back of the freezer 1. The connecting pipe 307 is connected to the output end of the pump body 306.

[0031] Example 2:

[0032] Based on Example 1, please refer to Figures 1-4 When the ground is uneven, the liquid nitrogen level inside the cryo-chamber 1 will vary, causing uneven cooling of the embryos. To ensure that the cryo-chamber 1 is level, this device is also equipped with an adjustment mechanism, which includes an upper support rod 401, a lower support rod 402, and a base 403. The upper support rod 401 is fixedly installed around the bottom of the base plate 202, and the lower support rod 402 is threaded to the lower end inside the upper support rod 401. The upper support rod 401 has an internal thread, and the lower support rod 402 has an external thread. When the uneven ground causes the base plate 202 to tilt, the lower support rod 402 is rotated to move up and down along the upper support rod 401, thereby adjusting the height of the four corners of the base plate 202. This keeps the cryo-chamber 1 level and prevents uneven liquid nitrogen levels inside the cryo-chamber 1 from causing uneven cooling of the wheat tubes and affecting the survival rate of the embryos. The base 403 is fixedly installed at the bottom of the lower support rod 402.

[0033] When in use, insert the embryo straw to be frozen into the placement hole of the placement plate 303, then hold the handle 305 and place the placement plate 303 into the freezer 1. When the support frame 304 contacts the top of the fixed frame 302, release the handle 305, close the top cover, and then start the pump body 306 to introduce liquid nitrogen from the right cavity into the left cavity through the connecting pipe 307 to freeze and preserve the embryo. When the ground vibrates, the freezer 1 pushes the rotating plate 207 to drive the connecting block 206 to compress the buffer spring 204 and the damping rod 205, thereby reducing the vibration of the freezer 1.

[0034] It should be noted that, in this document, relational terms such as "first" and "second" are used only to distinguish one entity or operation from another, and do not necessarily require or imply any such actual relationship or order between these entities or operations. Furthermore, the terms "comprising," "including," or any other variations thereof are intended to cover non-exclusive inclusion, such that a process, method, article, or apparatus that comprises a list of elements includes not only those elements but also other elements not expressly listed, or elements inherent to such a process, method, article, or apparatus. Without further limitations, an element defined by the phrase "comprising one..." does not exclude the presence of other identical elements in the process, method, article, or apparatus that includes said element.

Claims

1. An embryo cryopreservation device, comprising a cryostat (1), characterized in that: The freezer (1) is provided with a storage device at the bottom and inside, the storage device including a shock absorption mechanism, a freezing mechanism and an adjustment mechanism; The shock absorption mechanism is located at the bottom of the freezer (1), the freezing mechanism is located inside the freezer (1), and the adjustment mechanism is located at the bottom of the shock absorption mechanism; The shock absorption mechanism includes telescopic rods (201), a base plate (202), a mounting plate (203), a buffer spring (204), a damping rod (205), a connecting block (206), a rotating plate (207), and a connecting frame (208). The telescopic rods (201) are fixedly installed around the bottom of the freezer (1). The base plate (202) is fixedly installed at the bottom of the four telescopic rods (201). The mounting plate (203) is fixedly installed on the top left and right sides of the base plate (202). The buffer spring (204) is fixedly installed on the adjacent side of the two mounting plates (203). The damping rod (205) is installed inside the buffer spring (204). The connecting block (206) is fixedly installed at the end of the buffer spring (204) away from the mounting plate (203). One end of the rotating plate (207) is rotatably connected to the outside of the connecting block (206). The connecting frame (208) is fixedly installed at the bottom of the freezer (1).

2. The embryo cryopreservation device according to claim 1, characterized in that: The end of the rotating plate (207) away from the connecting block (206) is rotatably connected to the connecting frame (208).

3. The embryo cryopreservation device according to claim 1, characterized in that: The freezing mechanism includes a partition (301), a fixing frame (302), a placement plate (303), a support frame (304), a handle (305), a pump body (306), and a connecting pipe (307). The partition (301) is fixedly installed inside the freezer (1). The fixing frame (302) is fixedly installed on the left side inside the freezer (1). The placement plate (303) is located inside the freezer (1). The support frame (304) is fixedly installed on the front and rear sides of the top of the placement plate (303). The handle (305) is fixedly installed on the top of the support frame (304). The pump body (306) is fixedly installed on the back of the freezer (1). The connecting pipe (307) is connected to the output end of the pump body (306).

4. The embryo cryopreservation device according to claim 3, characterized in that: The freezer (1) is equipped with a top cover, and the partition (301) divides the freezer (1) into two cavities, with liquid nitrogen stored inside the right cavity.

5. The embryo cryopreservation device according to claim 3, characterized in that: The placement plate (303) has placement holes inside.

6. The embryo cryopreservation device according to claim 1, characterized in that: The adjustment mechanism includes an upper support rod (401), a lower support rod (402), and a base (403). The upper support rod (401) is fixedly installed around the bottom of the base plate (202). The lower support rod (402) is threadedly connected to the lower end of the upper support rod (401). The base (403) is fixedly installed at the bottom of the lower support rod (402).

7. The embryo cryopreservation device according to claim 6, characterized in that: The upper support rod (401) has an internal thread, and the lower support rod (402) has an external thread.

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