Novel cassette for pathological tissue dyeing and storage

Abstract

The utility model discloses a novel cassette for pathological tissue dyeing and storage, and belongs to pathological experiment auxiliary devices in the field of molecular biology experiment research. The cassette is composed of a dyeing device and a storage device. The dyeing device comprises a slide making box, a slide groove, a groove and other parts, multiple samples can be dyed and cleaned at the same time, and waste liquid can be conveniently discharged through a specific structure. The storage device is made of stainless steel or hard plastic materials, is provided with double-side storage inner grooves and slide clamping grooves, can be used for storing sample wafers in a dark place, and can be combined with the dyeing device or used independently. The staining cassette solves many problems of the existing staining cassette, is simple and convenient to operate, can improve the experimental efficiency and reduce experimental errors, and is suitable for staining and storing cell or pathological sample slides.

Description

Technical Field

[0001] This utility model relates to the field of molecular biology experimental research, specifically to auxiliary devices for pathological experiments, and in particular to a novel dark box for staining and storing pathological tissues. Background Technology

[0002] Since the 1990s, pathological examination, encompassing multiple fields such as immunology, molecular biology, and oncogene testing, has become an indispensable diagnostic tool in clinical work and scientific research. It not only assists in diagnosis—observing the nature of lesions under a microscope by preparing pathological sections with an accuracy rate of approximately 99%—but also guides treatment, prognosis, assists in surgical plan selection, and assesses treatment efficacy. In commonly used staining techniques in molecular biology laboratories, such as HE staining, immunohistochemistry, and immunofluorescence, the pretreatment of experimental materials is complex, involving steps such as fixation, dehydration, clearing, dewaxing, staining, dehydration and clearing, and mounting. Multiple reagents are required to treat the materials, and they must be repeatedly soaked and rinsed with water or PBS. Furthermore, the staining effect is affected by factors such as staining time, temperature, pH value of the staining solution, and effective concentration, and the entire experimental process must be conducted in a light-protected environment. However, there is currently no dedicated equipment for these staining techniques. Existing cell and molecular biology research experiments typically involve operators performing staining and cleaning operations one by one in a standard dark box. Staining and cleaning operations cannot be performed simultaneously, and samples need to be repeatedly handled, increasing the difficulty for researchers and prolonging the operation time. When staining large batches of samples, long staining intervals can easily lead to overstaining, resulting in significant variations in experimental results and unsatisfactory staining outcomes. Furthermore, existing staining cassettes are prone to tipping over, scattering, and sample confusion during use. Utility Model Content

[0003] 1. Purpose of the utility model: This utility model aims to overcome the shortcomings of the prior art and provide a novel, easy-to-use dark box for staining and storing pathological tissues. This dark box solves the problems of easy tipping and sample confusion in existing staining dark boxes, adds a fixing support to assist experimental operations, achieves light-proof operation, reduces the number of sample handling operations, simplifies the experimental process, improves experimental efficiency, and thus obtains better experimental results.

[0004] 2. Technical solution: The new type of dark box for staining and storing pathological tissues consists of two parts: a staining device and a storage device.

[0005] Staining apparatus: The slide box (1) is equipped with multiple slide slots (2) inside, the size of which matches the existing glass slides for easy sample placement. The grooves (3) on the slide slots (2) facilitate the placement and removal of glass slides. Each slide slot (2) is spaced 1 cm apart to avoid collisions during placement and removal. Two glass slides can be placed in the same slide slot (2). The waste liquid flow channel (4) in the middle allows waste liquid to flow into the box body during cleaning and then be discharged through the drain port (11), facilitating the recovery of harmful reagents. The slide rail (5) at the front of the box body is slidably connected to the roller base (803). The slide rod (6) is installed on the sliding roller (8) and is connected and hinged to the slide slot (2) through the inner core support (7). When the slide rod (6) is pushed to the bottom of a specific slide slot (2), rotating the slide rod (6) drives the eccentric rail (801) to rotate, which can lift the inner core support (7) and lift the slide slot (2) so that the waste liquid flows into the waste liquid flow channel (4). The lid (9) and handle A (10) on the upper part of the storage box facilitate opening and closing during operation. At the same time, the slide tray (2) has a specific tilt angle in the groove (3) to facilitate the pouring of waste liquid. A sealing structure is provided between the lid (9) of the staining device and the slide preparation box (1) to prevent reagent evaporation and external impurities from entering during the staining process. The slide preparation box (1) and the slide tray (2) are made of corrosion-resistant materials and can withstand the corrosion of reagents commonly used in staining experiments.

[0006] Storage device: The storage box (13) can be made of stainless steel or hard plastic. The storage box (13) and the slide slots (17) are made of corrosion-resistant materials to ensure that there is no deformation or corrosion after long-term contact with reagents, thus extending the service life of the device. The double-sided storage tanks on the outside of the door (14) have multiple slide slots (17) horizontally opened on the side walls. Their size is adapted to conventional slides to ensure stable placement of the samples. The handle on the door (14) makes it easy to close the door. The door (14) is made of light-shielding material, which can effectively block light when the door (14) is closed, thus playing a role in light-proof preservation of the samples in the storage tank. The rounded interlocking hanging rings (18) at the four corners of the side of the storage box allow the storage device to be used in conjunction with the slide box or to store the prepared samples separately, realizing multiple uses of one box.

[0007] Beneficial effects: This utility model has a simple structure, reasonable design, and easy operation. It can perform staining and cleaning operations on multiple samples simultaneously. The multiple sets of slide placement slots on the slide slot (2) can meet the needs of simultaneous processing of multiple samples and can also be used for sample incubation. Through the cooperation of slide rails, slide rods, inner core support pillars and sliding rollers, the lifting and lowering of a single set of slide slots (2) can be realized, which facilitates the switching of staining and cleaning operations, eliminates the need to repeatedly take out slides, saves experimental time, avoids sample confusion, reduces experimental errors, and improves the accuracy of experimental operations. Even if the sample is unlabeled or the label is unclear, the corresponding sample can be quickly found according to the position of the slide slot (2). Attached Figure Description

[0008] Figure 1 A schematic diagram of a pathological tissue slide storage device for a novel dark box for staining and storing pathological tissues, as proposed in this utility model.

[0009] Figure 2 A schematic diagram of the internal support lifting structure of a novel pathological tissue staining and storage cassette proposed in this utility model.

[0010] Figure 3 This is a schematic diagram of a pathological tissue slide storage device for a novel dark box for staining and storing pathological tissues, as proposed in this utility model.

[0011] Legend:

[0012] Slide box (1); slide slot (2); groove (3); waste liquid flow channel (4); slide rail (5); slide rod (6); inner core support (7); sliding roller (8); eccentric rail (801); roller ball (802); roller base (803); box cover (9); handle A (10); drain port (11); storage box (12); storage box body (13); door body (14); handle B (15); hanging ring base (16); slide slot (17); fitting hanging ring (18); sample slide (19). Detailed Implementation

[0013] To make the technical means, creative features, objectives and effects of this utility model easier to understand, the present utility model will be further described below in conjunction with specific embodiments.

[0014] In the description of this utility model, it should be noted that the terms "upper," "lower," "inner," "outer," "front end," "rear end," "both ends," "one end," and "the other end," etc., indicate the orientation or positional relationship based on the orientation or positional relationship shown in the accompanying drawings. They are used only for the convenience of describing this utility model and simplifying the description, and do not indicate or imply that the device or element referred to must have a specific orientation, or be constructed and operated in a specific orientation. Therefore, they should not be construed as limitations on this utility model. In addition, the terms "first" and "second" are used for descriptive purposes only and should not be construed as indicating or implying relative importance.

[0015] In the description of this utility model, it should be noted that, unless otherwise explicitly specified and limited, the terms "installed," "equipped with," and "connected," etc., should be interpreted broadly. For example, "connected" can be a fixed connection, a detachable connection, or an integral connection; it can be a mechanical connection or an electrical connection; it can be a direct connection or an indirect connection through an intermediate medium; it can be a connection within two components. Those skilled in the art can understand the specific meaning of the above terms in this utility model based on the specific circumstances.

[0016] Example

[0017] When using this novel dark box for staining and storing pathological tissues, the following detailed steps must be followed:

[0018] Sample loading and staining preparation: The experimenter first selects sample slides that meet the experimental requirements and carefully places them in the slide slots (2) of the slide preparation box (1). Each slide slot (2) can hold two slides. When placing the slides, ensure that they fall steadily into the grooves (3). The design of the grooves (3) facilitates subsequent handling and avoids collisions between the slides. After loading the samples, the experimenter uses a micropipette to accurately draw an appropriate amount of staining solution according to the experimental protocol and slowly and evenly drips it onto the samples. The amount and speed of the staining solution dripped must be strictly in accordance with the experimental specifications to ensure the consistency of the staining effect. After dripping, quickly tighten the box lid (9) to ensure a relatively closed staining environment and reduce the interference of external factors on the staining process.

[0019] Staining process in the dark: After tightening the lid (9), place the dark box in a suitable experimental environment for staining in the dark. The staining time depends on the specific experimental requirements. Different staining methods and sample types require different staining times, and the experimenter must strictly control the staining time. During the staining process, the dark box should be placed on a stable experimental table to avoid shaking and vibration, so as to ensure the uniformity of staining.

[0020] Waste Discharge and Sample Cleaning: After the staining time is over, the experimenter opens the box lid (9). At this time, the waste discharge operation begins. The experimenter holds the grip on the slide bar (6) and pulls the slide bar (6) smoothly. The slide bar (6) drives the sliding roller (8) to slide in the slide rail (5). Since the slide bar (6) is connected to the slide groove (2) through the inner core support (7) and hinged on the sliding roller (8), when the slide bar (6) moves to the slide groove (2) where the waste liquid needs to be discharged, the slide bar (6) is rotated. The rotation of the slide bar (6) drives the eccentric rail (801) to rotate together. The special structure of the eccentric rail (801) causes the inner core support (7) to be lifted upward, thereby lifting the slide groove (2) at the corresponding position. Since the end of the slide trough (2) near the inner core support (7) is lower than the end away from the inner core support (7) when the inner core support (7) lifts the bottom of the slide trough (2) to a specific angle, that is, close to the ground level or slightly higher than the end of the waste liquid flow channel (4), the staining solution in the slide trough (2) will flow naturally into the box through the waste liquid flow channel (4) and finally be discharged through the drain port (11). If harmful reagents are used in the experiment, they can be collected and recycled outside the drain port (11) to avoid environmental pollution.

[0021] After the waste liquid is discharged, the sample cleaning operation is performed. The experimenter uses sterile water and appropriate tools (such as dropper or pipette) to add sterile water into the slide holder (2) to clean the sample. During the cleaning process, it is necessary to ensure that the sterile water fully covers the sample to remove residual staining solution. The samples in each set of slide holders (2) are cleaned in turn. After cleaning one set, slide the slider (6) to restore the slide holder (2) to the horizontal position, and then perform the cleaning operation for the next set.

[0022] Subsequent staining and processing: After completing one round of cleaning, if the experiment requires multiple staining steps, slide the slider (6) again to make all slide slots (2) horizontal, and then add the next staining solution according to the sample loading and staining preparation steps to perform staining and cleaning operations. Repeat the above staining, waste liquid discharge, and cleaning process until all staining steps are completed.

[0023] Sample Storage: After staining and cleaning, the slides can be transferred to the storage device for storage after being covered with coverslips. Open the door (14) of the storage device and carefully place the slides into the slide slots (17) of the storage box (12). The double-sided storage inner groove design of the storage box (12) and the internal elastic components can stably fix the slides and prevent them from shaking or slipping during storage. After placing the slides, close the door (14) and use the handle on the door (14) to ensure that the door (14) is closed tightly, thus achieving light-proof preservation of the slides.

[0024] Combination and Separation of the Dark Box: Throughout the experiment, the staining device and the storage device can be used together by connecting them via the interlocking hanging ring (18) and the hanging ring base (16) to facilitate sample transfer during the experiment; or they can be used separately depending on the actual experimental site, sample quantity, and other requirements. For example, when the experimental sample quantity is small, the storage device can be used alone to store a small number of prepared sample slides; when the experiment only involves staining, the staining device can be used alone to improve the flexibility and convenience of the experiment.

[0025] The foregoing has shown and described the basic principles, main features, and advantages of this utility model. Those skilled in the art should understand that this utility model is not limited to the above embodiments. The embodiments and descriptions in the specification are merely illustrative of the principles of this utility model. Various changes and modifications can be made to this utility model without departing from its spirit and scope, and all such changes and modifications fall within the scope of the claims. The scope of protection of this utility model is defined by the appended claims and their equivalents.

Claims

1. A novel cassette for pathological tissue staining and storage, characterized by, The application relates to a staining device and a storage device. The staining device comprises a slide preparation box (1), a slide groove (2), a groove (3), a waste liquid flow channel (4), a slide way (5), a slide rod (6), an inner core support (7), a sliding roller (8), an eccentric rail (801), a roller steel ball (802), a roller base (803), a box cover (9), a handle A (10) and a liquid discharge port (11); the slide preparation box (1) is internally provided with a plurality of slide grooves (2) consistent with the size of existing glass slides, the slide grooves (2) are provided with grooves (3), the slide grooves (2) are spaced at a certain distance, two glass slides can be placed in the same slide groove (2) and a waste liquid flow channel (4) is arranged between the two glass slides; a slide way (5) is arranged at the front of the box body, the roller base (803) is slidably connected with the slide way (5), the slide rod (6) on the sliding roller (8) is connected with the slide groove (2) through the inner core support (7) and is hingedly arranged on the sliding roller (8), the upper portion of the storage box is provided with the box cover (9) and the handle A (10), and when the slide groove (2) is in the groove (3), the end close to the inner core support (7) is lower than the end far from the inner core support (7); The storage device comprises a storage box (12), a storage box body (13), a door body (14), a handle B (15), a hanging ring base (16), a slide card slot (17), an embedded hanging ring (18) and a sample (19); the storage box body (13) is made of stainless steel or hard plastic material, the door body (14) is internally provided with double-sided storage inner grooves on the outer side, a plurality of slide card slots (17) with the size consistent with conventional glass slides are horizontally arranged on the side walls of the storage inner grooves, and circular-arc embedded hanging rings (18) are arranged around the four corners of the side surface of the storage box body.

2. A novel cassette for staining and storing pathological tissues according to claim 1, characterized in that, In the staining device, when the waste liquid is poured, the inner core support (7) can lift the bottom to a certain angle, so that the waste liquid can flow into the waste liquid flow channel (4) smoothly.

3. A novel cassette for staining and storing pathological tissues according to claim 1, characterized in that, The door body (14) of the storage device is provided with a handle convenient for operation, so that the light can be effectively shielded when the door body (14) is closed, and the sample in the storage groove can be stored in the light-proof mode.

4. The novel cassette for staining and storing pathological tissues according to claim 1, characterized in that, The staining device and the storage device are connected through the embedded hanging ring (18) and the hanging ring base (16), and the two can be separately used according to actual use requirements.

5. The novel cassette for staining and storing pathological tissues according to claim 1, characterized in that, The slide preparation box (1), the slide groove (2) of the staining device and the storage box body (13), the slide card slot (17) of the storage device are all made of corrosion-resistant materials, so as to prevent the reagents from being corroded.

6. A novel cassette for staining and storing pathological tissues according to claim 1, characterized in that, A sealing structure is arranged between the box cover (9) and the slide preparation box (1) of the staining device, so as to prevent the reagents from volatilizing and foreign matters from entering during the staining process.

7. The novel cassette for staining and storing pathological tissues according to claim 1, wherein Elastic components for fixing the sample are arranged in the double-sided storage inner grooves of the storage device, so as to prevent the sample from shaking or sliding during the storage process.

8. The novel cassette for staining and storing pathological tissues according to claim 1, wherein A holding part convenient for operation is arranged on the slide rod (6) of the staining device, so as to facilitate the experimental personnel to push the slide rod (6) to lift the slide groove (2).

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