High-throughput detection device for monitoring water pollution toxicity types

Abstract

The utility model discloses a high throughput detection device for monitoring water body pollution toxicity types, which relates to the technical field of water body pollution type detection and comprises a detection unit, the detection unit comprises a photon detection darkroom, and a photon counting module is arranged in the photon detection darkroom and can detect luminous data of luminous bacteria and transmit the data to a data processor; the perforated plate is arranged in the photon detection darkroom and located above the photon counting module, and to-be-detected samples and photogenic bacteria are placed in holes of the perforated plate; the power supply module is electrically connected with the photon counting module and the data processor. The high-throughput detection device for monitoring the pollution toxicity types of the water body can detect the pollution toxicity types in a large batch of samples in time.

Description

TECHNICAL FIELD

[0001] The utility model relates to water body pollution kind detection technical field especially relates to a kind of high-throughput detection device for monitoring water body pollution toxicity kind. BACKGROUND

[0002] Toxicity pollutant composition in water body is different, and there are many kinds, and water body pollution toxicity kind mainly includes heavy metal, organic pollutant, antibiotic etc., and at present, in order to detect the pollution toxicity kind in water body, sample is generally collected on site, which is transported to laboratory, and then specific kind of toxicity pollution is detected, which lacks timeliness, often leading to pollution sample failure or result distortion;Moreover, the flux of the existing detection equipment is low, generally only single channel, and when a large number of samples are detected simultaneously, the detection efficiency is low.

[0003] Therefore, a technical scheme for timely detecting the pollution toxicity kind in a large number of samples is needed. UTILITY MODEL CONTENT

[0004] The utility model aims at providing a kind of high-throughput detection device for monitoring water body pollution toxicity kind to solve the problems existing in the prior art, and the pollution toxicity kind in a large number of samples can be detected in time.

[0005] To achieve the above object, the utility model provides the following scheme:

[0006] The utility model provides a kind of high-throughput detection device for monitoring water body pollution toxicity kind, comprising:

[0007] Detection unit, it includes photon detection darkroom, photon counting module is equipped in the photon detection darkroom, the luminous data of luminous bacteria can be detected, and data is transmitted to data processor;

[0008] Multi-well plate is set in the photon detection darkroom, and is located above the photon counting module, and the hole in the multi-well plate is used to place sample and luminous bacteria to be detected;

[0009] Power module is electrically connected with the photon counting module and data processor respectively.

[0010] Preferably, the photon detection darkroom is detection incubation bin, the detection incubation bin side wall is equipped with light shielding layer, the multi-well plate is set in detection incubation bin, and the photon counting module is equipped below the multi-well plate.

[0011] Preferably, the detection incubation bin top is opened, and the opening is hinged with light shielding bin door, the bin door is connected with electric push rod away from hinged point, and the electric push rod can drive the bin door to close the opening;The multi-well plate is located below the opening.

[0012] Preferably, the detection incubation chamber is fixed with an incubation support, and the multi-well plate is fixed on the incubation support.

[0013] Preferably, a heating sheet and a thermometer are attached to the outside of the multi-well plate, the heating sheet is connected with a power module, and the thermometer is connected with the data processor.

[0014] Preferably, the photon counting module comprises a photon counter, and the photon counter is connected with the data processor.

[0015] Preferably, the photon counting module further comprises a moving platform, the moving platform is located below the multi-well plate, the photon counter and the timer are arranged on the moving platform, and the moving platform can drive the photon counter to move below any hole of the multi-well plate.

[0016] Preferably, the moving platform comprises an X-direction guide rail arranged on one side of the multi-well plate, a first sliding block is arranged on the X-direction guide rail and close to one side of the multi-well plate, a Y-direction guide rail is arranged on the first sliding block, the Y-direction guide rail is located below the multi-well plate, a second sliding block is arranged on the Y-direction guide rail, and the photon counter is fixed on the second sliding block; and the first sliding block and the second sliding block are respectively connected with sliding block driving devices.

[0017] Preferably, the data processor is fixed on one end of the photon detection darkroom, and a display is arranged on the data processor.

[0018] Preferably, the data processor is connected with an alarm, and the alarm can give an audible or light alarm when the detection data exceeds a preset value.

[0019] Compared with the prior art, the utility model discloses the following technical effects:

[0020] The utility model discloses whole simple structure can move to the scene and carry out timely sampling and detection, through multi-well plate, different sample to be detected can be placed in its multiple holes respectively, then the luminous bacteria are added in the sample, the luminous data of luminous bacteria will change under the influence of different kinds of pollution toxins, thereby the luminous data of luminous bacteria are detected based on photon technology module, and are transmitted to the data processor and are analyzed, compare, and the kind of pollution toxin in sample can be detected, realizes the comprehensive screening of a large number of samples, broad spectrum toxicity, can realize the real-time continuous collection and measurement of large quantities of samples such as surface water body, sewage outlet and can be suitable for the off-line and on-line toxicity detection of laboratory. BRIEF DESCRIPTION OF DRAWINGS

[0021] In order to more clearly illustrate the technical solutions in the embodiments of the present application or the prior art, the drawings needed in the embodiments will be briefly introduced as follows. Obviously, the drawings in the following description are only some embodiments of the present application, and for those skilled in the art, other drawings can also be obtained from these drawings without creative labor.

[0022] Figure 1 The structure schematic diagram of the high-throughput detection device for monitoring the toxic species of water pollution in one or some embodiments of the present application;

[0023] Figure 2 The arrangement schematic diagram of the photon counting module and the multi-well plate in the present application;

[0024] Figure 3 The schematic diagram of the data processor module in the present application.

[0025] In the figure: 1-detection incubation bin, 2-bin door, 3-photon counter, 4-moving platform, 5-heating sheet, 6-multi-well plate, 7-display screen, 8-X guide rail, 9-Y guide rail, 10-data processor. DETAILED DESCRIPTION

[0026] The technical solutions in the embodiments of the present application will be described clearly and completely in combination with the drawings in the embodiments of the present application. Obviously, the described embodiments are only some embodiments of the present application, not all the embodiments. Based on the embodiments in the present application, all other embodiments obtained by those skilled in the art without creative labor are within the protection scope of the present application.

[0027] The purpose of the present application is to provide a high-throughput detection device for monitoring the toxic species of water pollution, so as to solve the problems in the prior art and detect the toxic species in a large number of samples in time.

[0028] In order to make the above-mentioned purposes, features and advantages of the present application more obvious and easy to understand, the present application will be further described in detail in combination with the drawings and specific embodiments.

[0029] The detection of toxic pollution species in water bodies is currently carried out by collecting samples and sending them to a laboratory for detection. However, the flux of existing detection equipment is low, generally only a single channel, and for a large number of samples, it cannot be detected at the same time, and the detection efficiency is low. In addition, although there are some on-site detection equipment for pollution sources, the on-site detection equipment can generally only detect a single pollution species, and the detection technology has few monitoring indexes and low accuracy, and cannot effectively deal with the diversity of pollution, cannot provide a basis for further laboratory detection, and can miss many potential pollutants and unknown pollutants; and directly sending samples to the laboratory for detection also has the problems of timeliness and cost, causing large-scale sampling detection to be impossible, and thus there is a problem of missed detection.

[0030] To solve this problem, the utility model provides a kind of high flux detection device of monitoring water pollution toxicity species, reference Figure 1 、 Figure 2 And Figure 3 As shown, including detection unit, it includes photon detection darkroom, photon counting module is equipped in photon detection darkroom, can detect the luminous data of luminous bacteria, and data is transmitted to data processor 10;Multi-well plate 6 is arranged in photon detection darkroom, and is located above photon counting module, and the hole of multi-well plate 6 is used to place sample and luminous bacteria to be detected;Power module is electrically connected with photon counting module and data processor 10 respectively. The power module of the embodiment adopts a storage battery, which is used to provide stable working power for the detection unit and the data processor 10. The data processor 10 of the embodiment adopts a computer, which includes a central processing unit, a communication unit, an alarm module and a display module. The alarm module of the embodiment adopts an alarm, and the display module of the embodiment adopts a display screen 7. The data processor 10 is integrated at one end of the photon detection darkroom, so that it can be moved to the scene with the photon detection darkroom for timely detection.

[0031] Generally, the pollutants are classified into inorganic pollution, heavy metal pollution, organic pollution and eutrophication pollution. The photobacteria have different responses to different pollutants. When the pollutant is inorganic pollution, the light intensity of the photobacteria decreases sharply with time and then reaches a steady state. When the pollutant is heavy metal pollution, the light intensity of the photobacteria decreases slowly with time and then reaches a steady state, and the light intensity ratio of the photobacteria does not reach a very weak state. When the pollutant is organic pollution, the light intensity of the photobacteria increases slowly with time and then reaches a steady state, because the activity of the photobacteria is strengthened by the appropriate organic matter, leading to reproduction. When the pollutant is eutrophication pollution, the light intensity of the photobacteria increases rapidly with time and then decreases sharply, because the activity of the photobacteria is rapidly strengthened by the eutrophication, leading to reproduction, and after the eutrophication is consumed, the activity of the photobacteria decreases and even dies, so the light intensity decreases rapidly. A mathematical model of the light intensity ratio of the photobacteria and the type of the pollutant is established by using a known central processing unit, and the type of the pollutant of the water sample is calculated according to the light intensity ratio of the photobacteria of the measured water sample, so that the main type of the pollutant can be obtained, which is a mature known technology, so no further description is given.

[0032] In order to enable the utility model to carry out the on-site pollution toxin type detection in time, the photon detection darkroom of the embodiment is a rectangular box structure detection incubation bin 1, the side wall of the detection incubation bin 1 is provided with a light shielding layer, a multi-well plate 6 is arranged on the incubation support fixed in the detection incubation bin 1, a photon counting module is arranged below the multi-well plate 6, the multi-well plate 6 is a cell culture plate, the holes of which are similar to the structure of test tubes, the multi-well plate 6 is transparent, which facilitates detection by the photon counting module at the bottom of the multi-well plate 6, and the multi-well plate 6 can be replaced by a plurality of test tubes arranged in sequence as needed. The detection incubation bin 1 is provided with an opening at the top, and a light shielding bin door 2 is hinged to the opening. The end of the bin door 2 away from the hinge point is connected to an electric push rod, which can drive the bin door 2 to close the opening. When the detection incubation bin 1 is working, the bin door 2 is closed to ensure airtightness and light shielding. The airtight environment can ensure the stability of the temperature, and the light shielding environment can ensure that the interference of external stray light is minimized. The multi-well plate 6 is located below the opening, which facilitates the placement and removal of samples.

[0033] In order to realize the accuracy of the detection result, a heating module is arranged outside the multi-well plate 6 in the embodiment. The heating module comprises a heating sheet 5 attached to the outer side of the multi-well plate 6 and a thermometer. The heating sheet 5 is connected to a power module, and the thermometer is connected to a data processor 10 for temperature control during the detection process.

[0034] The photon counting module of the embodiment comprises a photon counter 3 connected with a data processor 10, the photon counter 3 is of a known structure, the data processor 10 is a computer, which is equipped with a power switch, a physical emergency stop switch, USB and RS232 communication interfaces, and mature operation software for controlling the automatic detection process. In order to realize the detection of samples in any hole of the multi-well plate 6, the photon counting module is further provided with a moving platform 4 located below the multi-well plate 6, the photon counter 3 is arranged on the moving platform 4, and the moving platform 4 can drive the photon counter 3 to move below any hole of the multi-well plate 6.

[0035] In operation, the matched luminous bacteria kit is configured according to the instructions, the resuscitation liquid is added to the luminous bacteria freeze-dried powder, after successful resuscitation, the toxic control substance or the sample to be detected is diluted to a suitable concentration with physiological saline, and then mixed and added to the holes of the multi-well plate 6 in an amount of 200 μL per hole. The multi-well plate 6 is placed in the detection incubation bin 1, and then the power is turned on. The built-in known existing biological toxicity detection software is opened on the display screen 7, the main interface is clicked to set the incubation temperature, reaction time, toxicity type, positive and negative control information, and sample hole position information. After the setting is completed, the detection program is started, the bin door 2 of the detection incubation bin 1 is controlled to open and close, the incubation temperature is heated according to the setting, the reaction time is waited to end, the photon counter 3 moves below the corresponding hole, and the luminous intensity of the hole is collected. After the collection is completed, the device automatically calculates according to the collected signal value, and gives the detection results such as the toxicity type and the toxicity grade under the condition of the selected reference. The multi-well plate 6 can be used to detect multiple samples at the same time, realize high-throughput detection, significantly reduce the detection cost, and further improve the sampling coverage, screening capacity and detection efficiency.

[0036] In a specific embodiment, the moving platform 4 comprises an X-direction guide rail 8 arranged on one side of the multi-well plate 6, the first sliding block is arranged on one side of the multi-well plate 6, the Y-direction guide rail 9 is arranged on the first sliding block, the Y-direction guide rail 9 is located below the multi-well plate 6, the second sliding block is arranged on the Y-direction guide rail 9, and the photon counter 3 is fixedly arranged on the second sliding block; the first sliding block and the second sliding block are respectively connected with sliding block driving devices, the sliding block driving devices adopt nut and screw mechanisms in the embodiment, the nuts are connected with the first sliding block or the second sliding block, and the screw rod motor at one end of the screw rod drives the screw rod to rotate, so that the nut drives the corresponding first sliding block or second sliding block to move horizontally along the respective guide rail.

[0037] The central processing unit of the data processor 10, i.e., the computer, is in communication connection with the display screen 7 through a communication unit; the alarm is connected to the central processing unit. The central processing unit is used to receive data from the detection unit, process and analyze, generate a water quality evaluation report, and transmit the result to the display screen 7 through the communication unit. The display screen 7 can display the water quality detection result in an intuitive way (such as numbers, charts, images, etc.) for the user to refer to and make decisions. When the central processing unit completes the analysis of the water quality detection data, it will judge the detection result according to the preset standard or threshold value. If the detection data exceeds the safe range or does not meet the specific requirements, the central processing unit will send a trigger signal to the alarm. After receiving the signal, the alarm will immediately start the alarm mechanism and can send a warning to the user through sound, light, etc.

[0038] The utility model discloses utilize luminescent bacteria to detect the different toxicity kind degree in water sample, reflect toxin and kind in water body through the intensity of chemiluminescent signal to evaluate the toxin pollution degree of water quality. The chemiluminescent signal detected by the detection unit is received by the photoelectric transducer and converted into an electric signal, and then amplified and processed by the signal processing circuit, and finally output to the central processing unit for analysis.

[0039] The principle and implementation mode of the utility model are described by applying specific examples in the utility model. The above embodiment is only used to help understand the method and core idea of the utility model. Meanwhile, for the general technical personnel in the field, according to the idea of the utility model, the specific implementation mode and application range will be changed. In conclusion, the content of the specification should not be understood as the limitation of the utility model.

Claims

1. A high-throughput detection device for monitoring water bodies for toxic species, characterized in that: The utility model relates to a detection device for bioluminescent bacteria, comprising: a detection unit comprising a photon detection darkroom, wherein a photon counting module is arranged in the photon detection darkroom, capable of detecting bioluminescent bacteria data and transmitting the data to a data processor; a multi-well plate arranged in the photon detection darkroom and above the photon counting module, wherein the wells of the multi-well plate are used to place samples and bioluminescent bacteria to be detected; a power module electrically connected to the photon counting module and the data processor, respectively.

2. The high-throughput device for monitoring water pollution toxicity species according to claim 1, characterized in that: The photon detection darkroom is a detection incubation chamber, and the side wall of the detection incubation chamber is provided with a light shielding layer. The multi-well plate is arranged in the detection incubation chamber, and the photon counting module is arranged below the multi-well plate.

3. The high-throughput device for monitoring water bodies for toxic species according to claim 2, wherein: The top of the detection incubation chamber is open, and a light shielding chamber door is hinged to the opening. The end of the chamber door away from the hinge point is connected to an electric push rod, which can drive the chamber door to close the opening. The multi-well plate is located below the opening.

4. The high-throughput device for monitoring water pollution toxicity species according to claim 2, characterized in that: An incubation support is fixedly arranged in the detection incubation chamber, and the multi-well plate is fixed to the incubation support.

5. The high-throughput device for monitoring water pollution toxicity species according to claim 4, characterized in that: A heating sheet and a thermometer are attached to the outside of the multi-well plate. The heating sheet is connected to a power module, and the thermometer is connected to the data processor.

6. The high-throughput device for monitoring water bodies for toxic species according to claim 1, wherein: The photon counting module comprises a photon counter connected to the data processor.

7. The high-throughput device for monitoring water bodies for toxic species according to claim 6, wherein: The photon counting module further comprises a moving platform located below the multi-well plate. The photon counter is arranged on the moving platform, and the moving platform can drive the photon counter to move below any well of the multi-well plate.

8. The high-throughput device for monitoring water bodies for toxic species according to claim 7, wherein: The moving platform comprises an X-direction guide rail arranged on one side of the multi-well plate. A first sliding block is slidingly arranged on the side of the X-direction guide rail close to the multi-well plate. A Y-direction guide rail is arranged on the first sliding block and located below the multi-well plate. A second sliding block is arranged on the Y-direction guide rail, and the photon counter is fixedly arranged on the second sliding block. The first sliding block and the second sliding block are respectively connected to sliding block driving devices.

9. The high-throughput device for monitoring water bodies for toxic species according to claim 1, wherein: The data processor is fixed to one end of the photon detection darkroom, and a display is arranged on the data processor.

10. The high-throughput device for monitoring water bodies for toxic species according to claim 1, wherein: The data processor is connected to an alarm. When the detection data exceeds a preset value, the alarm can issue a sound or light alarm.

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