Nine-way valve for plasmid cracking device
By designing a nine-way valve for the plasmid lysis device, multiple flow path switching and flow rate control are achieved, solving the problem that existing equipment cannot accurately control the plasmid lysis time, and improving the efficiency and quality of plasmid extraction.
Patent Information
- Application Number
- CN202520576523.4
- Authority / Receiving Office
- CN · China
- Patent Type
- Utility models(China)
- Current Assignee / Owner
- Filing Date
- 2025-03-29
- Publication Date
- 2026-01-27
- Estimated Expiration
- 2035-03-29
AI Technical Summary
Existing equipment cannot effectively control plasmid lysis time, making it difficult to screen for suitable lysis times and failing to meet the needs of plasmid extraction.
Design a nine-way valve for a plasmid lysis device to achieve multiple flow path switching. Combined with a pump, change the flow rate to control the lysis time. By switching between different lysis sections through the nine-way valve, multiple lysis fields are provided so as to select a suitable lysis time.
It enables precise control of plasmid lysis time, improves the efficiency and quality of plasmid extraction, and meets different experimental needs.
Smart Images
Figure CN223839802U_ABST
Abstract
Description
Technical Field
[0001] This utility model relates to the field of biotechnology equipment, specifically to a nine-way valve for a plasmid lysis device. Background Technology
[0002] Directional control valves are commonly used in analytical and research equipment in the biopharmaceutical field, primarily to alter the flow path of solutions. Examples include three-way valves and six-way valves, with six-way valves being particularly prevalent in laboratory instruments such as high-performance liquid chromatographs (HPLC). When improving equipment, the first thought is often to use common components; however, in some cases, new parts must be designed to better solve the problem.
[0003] Plasmids are common raw materials in gene therapy, generally obtained from cultured bacteria. The lysis of plasmids requires screening for suitable processes, with lysis time being a crucial process condition. The lysis buffer is mixed with the disrupted bacterial cells, and after a certain time, a neutralizing solution is added to terminate the lysis. Only by controlling the appropriate time can the desired plasmid be obtained. Currently, there is no suitable equipment for screening plasmid lysis times.
[0004] Generally, the contact time between the lysis solution and the plasmid is controlled by extending the length of the lysis tubing. Therefore, a reversing valve that can switch between different lysis tubing is needed. Utility Model Content
[0005] To achieve the above-mentioned objectives, this invention provides a nine-way valve for a plasmid pyrolysis device. This nine-way valve can realize multiple flow paths, has a high degree of integration, a simple structure, and a small size.
[0006] This utility model discloses a nine-way valve for a plasmid lysis device. The nine-way valve includes a valve body, a valve core that can rotate inside the valve body, and an end cap for sealing the valve core and fixing it on the valve body. The valve body has nine ports. The valve core is a cylinder. Three passages are provided on the upper surface of the valve core, two of which are joined at one end to form a single port. Two passages are provided on the lower surface of the valve core. Each passage on the valve core can connect to two ports on the valve body.
[0007] Compared with the prior art, this utility model realizes the integration of nine ports on one valve, enabling the switching of multiple working modes. Attached Figure Description
[0008] Figure 1 Schematic diagram of the structure of this utility model
[0009] Figure 2 The three passages on the upper surface of the valve core of this utility model are AC passage, MC passage, and HE passage;
[0010] Figure 3 The two passages on the lower surface of the valve core of this utility model are the fk passage and the bd passage.
[0011] Figure 4 The first working mode of this utility model;
[0012] Figure 5 The second working mode of this utility model;
[0013] Figure 6 The third working mode of this utility model;
[0014] Figure 7 The fourth working mode of this utility model.
[0015] In the figure, there are: first port 1, second port 2, third port 3, fourth port 4, fifth port 5, sixth port 6, seventh port 7, eighth port 8, ninth port 9, valve body 10, valve core 11, end cap 12, first pyrolysis section 13, second pyrolysis section 14, third pyrolysis section 15, and fourth pyrolysis section 16. Specific Implementation
[0016] like Figure 1-3 As shown, a nine-way valve for a plasmid lysis apparatus is disclosed. The nine-way valve includes a valve body 10, a valve core 11 rotatable within the valve body 10, and an end cap 12 for sealing the valve core 11 and fixing it to the valve body 10. The valve body 10 has nine ports. The valve core 11 is a cylinder with three passages on its upper surface, two of which merge into one port at one end. Two passages are provided on the lower surface of the valve core 11. Each passage on the valve core 11 can connect to two ports on the valve body 10.
[0017] The nine ports on the valve body 10 are designated as first port 1, second port 2, third port 3, fourth port 4, fifth port 5, sixth port 6, seventh port 7, eighth port 8, and ninth port 9; among which, first port 1 is the inlet.
[0018] The three passages on the upper surface of the valve core 11 are the ac passage, the mc passage, and the he passage, and the two passages on the lower surface of the valve core 11 are the fk passage and the bd passage.
[0019] The second port 2 is connected to the fourth port 4 and flows into the outlet pipeline; the third port 3 is connected to the fifth port 5; the sixth port 6 is connected to the seventh port 7; and the eighth port 8 is connected to the ninth port 9.
[0020] In the first working mode of the nine-way valve, the MC passage connects the first port 1 and the fourth port 4.
[0021] In the second working mode of the nine-way valve, the ac passage connects the first port 1 and the third port 3; the bd passage connects the fifth port 5 and the second port 2.
[0022] In the third working mode of the nine-way valve, the he passage connects the first port 1 and the eighth port 8, and the fk passage connects the ninth port 9 and the second port 2.
[0023] In the fourth working mode of the nine-way valve, the fk passage connects the first port 1 and the eighth port 8, the he passage connects the ninth port and the seventh port, the bd passage connects the sixth port 6 and the third port 3, and the mc passage connects the fifth port 5 and the second port 2.
[0024] A plasmid lysis apparatus equipped with a nine-way valve includes,
[0025] The liquid addition mechanism is used to add bacterial suspension and lysis buffer to the plasmid lysis device;
[0026] A separation and mixing mechanism is used to separate plasmids from the initial bacterial suspension and mix plasmids with lysate.
[0027] The lysis mechanism includes a lysis section that provides a site for lysis of plasmids and lysis buffer, and a switching section that communicates with the lysis section;
[0028] The neutralization mechanism is used to add neutralizing agent to the pyrolysis mixture;
[0029] Collection mechanism for collecting the neutralized mixture;
[0030] The pyrolysis mechanism connects the liquid addition mechanism and the neutralization mechanism;
[0031] The separation and mixing mechanism is located between the liquid addition mechanism and the pyrolysis mechanism;
[0032] The pyrolysis section includes a first pyrolysis section 13, a second pyrolysis section 14, a third pyrolysis section 15, and a fourth pyrolysis section 16. The switching section is a nine-way valve, which allows the pyrolysis sections to be connected individually or in series by switching the nine-way valve.
[0033] The bacterial suspension to be lysed and the pre-prepared lysis buffer are added through the liquid addition mechanism, and then mixed and separated in the separation and mixing mechanism; the mixed liquid enters the lysis mechanism for lysis; the lysed liquid reacts with the neutralizing liquid in the neutralization mechanism to stop the lysis, and the neutralized liquid enters the collection mechanism for collection.
[0034] The pyrolysis mechanism includes a pyrolysis section and a switching section. There are multiple pyrolysis sections, providing multiple pyrolysis sites. By switching the directional valve to the desired pyrolysis section, different pyrolysis times can be achieved, thus enabling the selection of pyrolysis times. Alternatively, the pyrolysis time can also be changed by altering the flow rate using a pump. However, under the same flow rate conditions, the length of the pyrolysis section determines the pyrolysis time.
[0035] like Figure 4 As shown, when the nine-way valve is in its initial state, one end of the MC passage is connected to the first port 1, and the other end is connected to the fourth port 4, and the first pyrolysis section 13 is working.
[0036] like Figure 5 As shown, rotating the valve core 11 of the nine-way valve connects one end of the ac passage to the first port 1 and the other end to the third port 3; one end of the bd passage connects to the fifth port 5 and the other end to the second port 2, and the first pyrolysis section 13 and the second pyrolysis section 14 work together.
[0037] like Figure 6 As shown, rotating the valve core 11 of the nine-way valve causes one end of the he passage to be connected to the first port 1 and the other end to be connected to the eighth port 8, and one end of the fk passage to be connected to the ninth port 9 and the other end to be connected to the second port 2. The first pyrolysis section 13, the third pyrolysis section 15, and the fourth pyrolysis section 16 work together.
[0038] like Figure 7 As shown, rotating the valve core 11 of the nine-way valve connects one end of the fk passage to the first port 1 and the other end to the eighth port 8; one end of the he passage connects to the ninth port and the other end to the seventh port; one end of the bd passage connects to the sixth port 6 and the other end to the third port 3; one end of the mc passage connects to the fifth port 5 and the other end to the second port 2; and the first pyrolysis section 13, the second pyrolysis section 14, the third pyrolysis section 15, and the fourth pyrolysis section 16 work together.
Claims
1. A nine-way valve for a plasmid lysis apparatus, characterized in that, The nine-way valve includes a valve body (10), a valve core (11) that can rotate inside the valve body (10), and an end cap (12) for sealing the valve core (11) and fixing it on the valve body (10). The valve body (10) has nine ports. The valve core (11) is a cylinder. Three passages are provided on the upper surface of the valve core (11), and one end of two passages merges into one port. Two passages are provided on the lower surface of the valve core (11). Each passage on the valve core (11) can connect to two ports on the valve body (10).
2. The nine-way valve for a plasmid pyrolysis apparatus according to claim 1, characterized in that, The nine ports on the valve body (10) are the first port (1), the second port (2), the third port (3), the fourth port (4), the fifth port (5), the sixth port (6), the seventh port (7), the eighth port (8), and the ninth port (9); The first pipe opening (1) is the inlet.
3. A nine-way valve for a plasmid lysis apparatus according to claim 2, characterized in that, The three passages on the upper surface of the valve core (11) are the ac passage, the mc passage, and the he passage, and the two passages on the lower surface of the valve core (11) are the fk passage and the bd passage.
4. A nine-way valve for a plasmid pyrolysis apparatus according to claim 3, characterized in that, The second port (2) is connected to the fourth port (4) and merges into the outlet pipeline; the third port (3) is connected to the fifth port (5); the sixth port (6) is connected to the seventh port (7); and the eighth port (8) is connected to the ninth port (9).
5. A nine-way valve for a plasmid pyrolysis apparatus according to claim 4, characterized in that, In the first working mode of the nine-way valve, the mc passage connects the first port (1) and the fourth port (4).
6. A nine-way valve for a plasmid pyrolysis apparatus according to claim 5, characterized in that, In the second working mode of the nine-way valve, the ac passage connects the first port (1) and the third port (3); the bd passage connects the fifth port (5) and the second port (2).
7. A nine-way valve for a plasmid lysis apparatus according to claim 6, characterized in that, In the third working mode of the nine-way valve, the he passage connects the first port (1) and the eighth port (8), and the fk passage connects the ninth port (9) and the second port (2).
8. A nine-way valve for a plasmid pyrolysis apparatus according to claim 7, characterized in that, In the fourth working mode of the nine-way valve, the fk passage connects the first port (1) and the eighth port (8), the he passage connects the ninth port and the seventh port, the bd passage connects the sixth port (6) and the third port (3), and the mc passage connects the fifth port (5) and the second port (2).