Zebra fish behavioral analysis device

Abstract

The utility model provides a zebra fish behavioral analysis device which comprises a box body, and a box cover is hinged to the box body. The interior of the box body is divided into a three-box social contact area, a novel water tank experiment area, a T labyrinth area, a Y labyrinth area and an open field area by partition plates; two first plate frames are arranged in the three-box social contact area, and through holes are formed in the first plate frames; the Y labyrinth area is provided with a Y-shaped channel defined by a second plate frame. The T-shaped labyrinth area is provided with a T-shaped channel defined by a third plate frame. Grid lines are drawn at the bottom of the open field area; grooves corresponding to the three-box social contact area, the novel air box experiment area, the T labyrinth area, the Y labyrinth area and the open field area are formed in the box cover, and cameras are arranged in the grooves; the camera is electrically connected with a touch control module arranged outside the box body, and animal behavior analysis software is arranged in the touch control module. The zebra fish behavioristics analysis device provided by the utility model can accurately record and analyze various behavioristics of the zebra fish, and further facilitates research and development in the fields of neurobiology, environmental toxicology and the like.

Description

TECHNICAL FIELD

[0001] The utility model belongs to experimental apparatus technical field, concretely relates to a zebra fish behavior analysis device. BACKGROUND

[0002] Zebrafish as biomedical research model organism, because its unique biological characteristics and experimental advantages, in the field of neurobiology, behavior, genetics and drug screening has been widely used.

[0003] The existing zebra fish behavior analysis system is divided into several modules, including camera and support, computer analysis end, behavior hardware three modules, three modules not only occupy larger space, and there are various drawbacks in hardware facilities, such as air storage box is generally adopted transparent acrylic plate, greatly increase the influence of the outside experimental animal.

[0004] Therefore, a new integrated experimental box for recording and analyzing various behaviors of zebra fish is needed to meet the actual needs and promote the development of neurobiology, environmental toxicology and other fields. UTILITY MODEL CONTENT

[0005] The utility model aims at the shortage of prior art, provides a zebra fish behavior analysis device, and the specific scheme is as follows:

[0006] A zebra fish behavior analysis device, including the box, the box is hinged with lid, the lid is equipped with handle, and the box is equipped with a plurality of supporting legs; the box is divided into three boxes social area, new water tank experimental area, T maze area, Y maze area and open field area by partition; two plate frames are arranged in the three boxes social area, and the plate frame is provided with a through hole; the Y maze area is provided with a Y-shaped channel surrounded by a plate frame two; the T maze area is provided with a T-shaped channel surrounded by a plate frame three; the bottom of the open field area is drawn with a square grid line; the lid is provided with a recess corresponding to the three boxes social area, new water tank experimental area, T maze area, Y maze area and open field area, and a camera is arranged in the recess; the camera is electrically connected with a touch module arranged outside the box, and the touch module is provided with animal behavior analysis software.

[0007] Based on the above, the bottom of the box is provided with at least one drain corresponding to the three boxes social area, new water tank experimental area, T maze area, Y maze area and open field area, and a sealing plug is arranged at the drain.

[0008] Based on the above, a jet pipe with one end sealed is provided through and rotatably mounted on the housing. The jet pipe is provided with multiple nozzles. A fan is provided outside the housing. The inlet of the fan is connected to one end of the air filter assembly. The outlet of the fan is connected to the airflow transmission assembly through a connecting pipe. The airflow transmission assembly is associated with the jet pipe. The fan is electrically connected to the touch module.

[0009] Based on the above, the airflow transmission assembly includes a cylindrical outer shell disposed outside the housing. The side wall of the outer shell is provided with an air inlet connected to the connecting pipe one. The orientation of the air inlet is offset from the axis of the outer shell. One end of the outer shell is also provided with an air outlet. A rotating rod is rotatably disposed through the axis of the outer shell. The rotating rod is provided with multiple fan blades. One end of the rotating rod is provided with a turntable. The turntable is provided with a first pin. The first pin is rotatably connected to one end of a connecting plate. The other end of the connecting plate is provided with a second pin. The second pin is rotatably connected to one end of a transmission rod. A support rod is fixed outside the housing. The support rod is provided with a guide frame for the transmission rod. The transmission rod is provided with a rack that meshes with a driven gear fixed on a jet pipe. The air outlet is connected to one end of the connecting pipe two. The other end of the connecting pipe two is rotatably connected to the open end of the jet pipe through a rotary joint.

[0010] Based on the above, the lid of the box is equipped with at least one ultraviolet lamp electrically connected to the touch module for each of the three-box social area, the new water tank experimental area, the T-maze area, the Y-maze area, and the open area.

[0011] Based on the above, the box body, box cover, and partition are all made of opaque board with vacuum interlayer. The box cover is equipped with lighting lights that are electrically connected to the touch module, corresponding to the three social areas, the new water tank experimental area, the T-maze area, the Y-maze area, and the open area.

[0012] Based on the above, the brightness adjustment range of the lighting lamp is 10-400 lux.

[0013] This utility model has substantial features and progress compared to the prior art. Specifically, this utility model has the following advantages:

[0014] The zebrafish behavioral analysis device provided by this utility model has a structural design that facilitates the recording and analysis of various behaviors of zebrafish, and promotes the development of fields such as neurobiology and environmental toxicology. In addition, the device is easy to clean, sterilize and maintain, and its structural design is ingenious and practical. Attached Figure Description

[0015] Figure 1 This is a schematic diagram of the overall structure of this utility model.

[0016] Figure 2This is a top view of the box body in this utility model.

[0017] Figure 3 yes Figure 1 Schematic diagram of the structure at point A in the middle.

[0018] In the diagram: 1. Box body; 1-1. Partition plate; 2. Box lid; 2-1. Groove; 3. Support leg; 4. Three-box social area; 4-1. Plate rack one; 5. New type of water tank experimental area; 6. T-maze area; 6-1. Plate rack two; 7. Y-shaped maze area; 7-1. Frame 3; 8. Open space area; 9. Camera; 10. Lighting; 11. Ultraviolet lamp; 12. Jet pipe; 13. Fan; 14. Air filter assembly; 15. Connecting pipe 1; 16. Airflow transmission assembly; 16-1. Housing; 16-2. Air inlet; 16-3. Air outlet; 16-4. Rotating rod; 16-5. Fan blade; 16-6. Turntable; 16-7. Connecting plate; 16-8. Transmission rod; 16-9. Guide frame; 16-10. Rack; 17. Connecting pipe 2; 18. Driven gear; 19. Touch module; 20. Drain outlet; 21. Handle. Detailed Implementation

[0019] The technical solution of this utility model will be further described in detail below through specific embodiments. Example

[0020] like Figures 1-3 As shown, this utility model provides a zebrafish behavioral analysis device, including a box body 1, a box cover 2 hinged to the box body 1, a handle 21 on the box cover 2, and multiple support legs 3 at the bottom of the box body 1; the box body 1 is divided into three social areas 4, a novel water tank experimental area 5, a T-maze area 6, a Y-maze area 7, and an open area 8 by a partition plate 1-1; the three social areas 4 are provided with two plate frames 4-1, and the plate frames 4-1 are provided with through holes; the Y-maze area 7 is provided with a plate frame 4-1. The Y-shaped passage is formed by the second frame 6-1; the T-maze area 6 is provided with a T-shaped passage formed by the third frame 7-1; the bottom of the open area 8 is drawn with square lines; the box cover 2 is provided with grooves 2-1 corresponding to the three-box social area 4, the new water tank experimental area 5, the T-maze area 6, the Y-maze area 7 and the open area 8, and a camera 9 is provided in the groove 2-1; the camera 9 is electrically connected to the touch module 19 set outside the box body 1, and the touch module 19 has built-in animal behavior analysis software.

[0021] To facilitate the drainage of water from the tank 1, at least one drain outlet 20 is provided at the bottom of the tank 1 corresponding to the three-tank social area 4, the new water tank experimental area 5, the T-maze area 6, the Y-maze area 7, and the open area 8. Each drain outlet 20 is equipped with a sealing plug.

[0022] When not in use, the device has a jet pipe 12 that is sealed at one end and rotatably installed through the housing 1 to facilitate drying of the inside of the housing 1. The jet pipe 12 is equipped with multiple nozzles. A fan 13 is installed outside the housing 1. The inlet of the fan 13 is connected to one end of the air filter assembly 14. The outlet of the fan 13 is connected to the airflow transmission assembly 16 through a connecting pipe 15. The airflow transmission assembly 16 is associated with the jet pipe 12. The fan 13 is electrically connected to the touch module 19.

[0023] The aforementioned airflow transmission assembly 16 includes a cylindrical outer shell 16-1 disposed outside the housing 1. The side wall of the outer shell 16-1 has an air inlet 16-2 connected to the connecting pipe 15. The orientation of the air inlet 16-2 is offset from the axis of the outer shell 16-1. One end of the outer shell 16-1 also has an air outlet 16-3. A rotating rod 16-4 is rotatably mounted through the axis of the outer shell 16-1. Multiple fan blades 16-5 are mounted on the rotating rod 16-4. A turntable 16-6 is mounted on one end of the rotating rod 16-4. A pin is mounted on the turntable 16-6. One end of the connecting plate 16-7 is rotatably connected to the other end of the connecting plate 16-7. The other end of the connecting plate 16-7 is provided with a second pin. The second pin is rotatably connected to one end of the transmission rod 16-8. A support rod is fixed to the outside of the housing 1. The support rod is provided with a guide frame 16-9 for the transmission rod 16-8. The transmission rod 16-8 is provided with a rack 16-10 that meshes with the driven gear 18 fixed on the jet pipe 12. The air outlet 16-3 is connected to one end of the second connecting pipe 17. The other end of the second connecting pipe 17 is rotatably connected to the open end of the jet pipe 12 through a rotary joint.

[0024] After the experiment, the inside of the chamber 1 needs to be cleaned. After cleaning, in order to facilitate the drying of the inside of the chamber, the fan 13 is turned on. The outside airflow is filtered by the air filter assembly 14 and enters the outer shell 16-1 through the connecting pipe 15. The fan blades 16-5 are blown to make the rotating rod 16-4 rotate. When the rotating rod 16-4 rotates, the turntable 16-6 rotates, which in turn drives the transmission rod 16-8 to move up and down through the connecting plate 16-7. The rack 16-10 on the transmission rod 16-8 drives the driven gear 18 to rotate back and forth, which in turn makes the jet pipe 12 rotate back and forth. The airflow inside the outer shell 16-1 enters the jet pipe 12 through the connecting pipe 2 17. Finally, the airflow is sprayed out from the nozzle to dry the inside of the chamber 1.

[0025] To facilitate disinfection and sterilization of the device's interior, at least one ultraviolet lamp 11 electrically connected to the touch module 19 is provided on the lid 2 corresponding to the three-box social area 4, the new water tank experimental area 5, the T-maze area 6, the Y-maze area 7, and the open area 8.

[0026] To facilitate lighting inside the tank 1 so that the camera 9 can capture images of the zebrafish's behavior, the tank 1, the lid 2, and the partition 1-1 are all made of opaque board with a vacuum interlayer. The lid 2 is equipped with lights 10 that are electrically connected to the touch module 19, corresponding to the three-tank social area 4, the new water tank experimental area 5, the T-maze area 6, the Y-maze area 7, and the open area 8.

[0027] Considering that lighting can have a certain impact on the behavior of zebrafish, the brightness adjustment range of the above-mentioned lighting lamp 10 is 10-400 lux.

[0028] The zebrafish behavioral analysis device provided by this utility model has a structural design that facilitates the recording of various zebrafish behaviors via camera and transmission to the control module for analysis, which is conducive to promoting the development of fields such as neurobiology and environmental toxicology. In addition, the device is also easy to clean, sterilize and maintain, and its structural design is ingenious and practical.

[0029] Finally, it should be noted that the above embodiments are only used to illustrate the technical solution of this utility model and not to limit it; although the utility model has been described in detail with reference to preferred embodiments, those skilled in the art should understand that modifications can still be made to the specific implementation of this utility model or equivalent substitutions can be made to some technical features without departing from the spirit of the technical solution of this utility model, and all such modifications and substitutions should be covered within the scope of the technical solution claimed by this utility model.

Claims

1. A zebrafish behavioral analysis device, characterized in that: The system includes a box body (1), a box cover (2) hinged to the box body (1), a handle (21) on the box cover (2), and multiple support legs (3) below the box body (1). The box body (1) is divided into three social areas (4), a new type of water tank experimental area (5), a T-maze area (6), a Y-maze area (7), and an open area (8) by a partition (1-1). The three social areas (4) are provided with two board frames (4-1), and the board frames (4-1) are provided with through holes. The Y-maze area (7) is surrounded by board frames (6-1). Y-shaped passage; the T-maze area (6) is provided with a T-shaped passage formed by three board frames (7-1); the bottom of the open area (8) is drawn with grid lines; the box cover (2) is provided with grooves (2-1) corresponding to the three-box social area (4), the new water tank experimental area (5), the T-maze area (6), the Y-maze area (7) and the open area (8), and a camera (9) is provided in the groove (2-1); the camera (9) is electrically connected to the touch module (19) set outside the box body (1), and the touch module (19) has built-in animal behavior analysis software.

2. The zebrafish behavioral analysis device according to claim 1, characterized in that: The bottom of the box (1) is provided with at least one drain outlet (20) corresponding to the three-box social area (4), the new water tank experimental area (5), the T-maze area (6), the Y-maze area (7) and the open area (8), and the drain outlet (20) is provided with a sealing plug.

3. The zebrafish behavioral analysis device according to claim 1, characterized in that: The housing (1) is provided with a jet pipe (12) that is sealed at one end and rotates through it. The jet pipe (12) is provided with multiple nozzles. A fan (13) is provided outside the housing (1). The inlet of the fan (13) is connected to one end of the air filter assembly (14). The outlet of the fan (13) is connected to the airflow transmission assembly (16) through the connecting pipe (15). The airflow transmission assembly (16) is associated with the jet pipe (12). The fan (13) is electrically connected to the touch module (19).

4. The zebrafish behavioral analysis device according to claim 3, characterized in that: The airflow transmission assembly (16) includes a cylindrical outer shell (16-1) disposed outside the housing (1). The side wall of the outer shell (16-1) is provided with an air inlet (16-2) connected to the connecting pipe (15). The orientation of the air inlet (16-2) is offset from the axis of the outer shell (16-1). One end of the outer shell (16-1) is also provided with an air outlet (16-3). A rotating rod (16-4) is rotatably mounted through the axis of the outer shell (16-1). Multiple fan blades (16-5) are mounted on the rotating rod (16-4). A turntable (16-6) is mounted at one end of the rotating rod (16-4). A pin is mounted on the turntable (16-6). The first pin is rotatably connected to one end of the connecting plate (16-7), and the other end of the connecting plate (16-7) is provided with a second pin. The second pin is rotatably connected to one end of the transmission rod (16-8). A support rod is fixed outside the housing (1). A guide frame (16-9) for the transmission rod (16-8) is provided on the support rod. A rack (16-10) that meshes with the driven gear (18) fixed on the jet pipe (12) is provided on the transmission rod (16-8). The air outlet (16-3) is connected to one end of the second connecting pipe (17). The other end of the second connecting pipe (17) is rotatably connected to the open end of the jet pipe (12) through a rotary joint.

5. The zebrafish behavioral analysis device according to claim 1, characterized in that: The lid (2) is provided with at least one ultraviolet lamp (11) electrically connected to the touch module (19) for each of the three-box social area (4), the new water tank experimental area (5), the T-maze area (6), the Y-maze area (7) and the open area (8).

6. The zebrafish behavioral analysis device according to claim 1, characterized in that: The box body (1), the box cover (2) and the partition (1-1) are all made of opaque board with vacuum interlayer. The box cover (2) is equipped with lighting lamps (10) that are electrically connected to the touch module (19) for the three-box social area (4), the new water tank experimental area (5), the T maze area (6), the Y maze area (7) and the open area (8).

7. The zebrafish behavioral analysis device according to claim 6, characterized in that: The brightness adjustment range of the lighting lamp (10) is 10-400 lux.

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