Mesenchymal stem cell separation and amplification kit

Abstract

The utility model relates to the technical field of kits, and provides a mesenchymal stem cell separation and amplification kit which comprises a kit body, a cover plate is arranged at the top of the kit body, a driving piece is arranged at the bottom in the kit body, a rectangular block is fixedly connected to the top end of the driving piece, and driving assemblies are arranged on the four side faces of the rectangular block; according to the mesenchymal stem cell separation and amplification kit disclosed by the utility model, the driving component can rotate by arranging the driving piece, so that the driving component collides with the extrusion block and the ball on the inner wall of the kit; under the matching action of a connecting rod, a rectangular rod, a hollow rod, a rectangular groove, a spring, a rectangular frame, a test tube sleeve, an extrusion plate, a spring I and a collision block, cell tissue fluid in a reagent tube can shake up and down and left and right by virtue of the restoring force of the spring and the spring I, so that the cell tissue fluid is never effectively prevented from precipitating.

Description

TECHNICAL FIELD

[0001] The utility model relates to the technical field of reagent box, especially a mesenchymal stem cell separation and expansion kit. BACKGROUND

[0002] The reagent box is a box for containing chemical reagents for detecting chemical components, drug residues, virus types, etc. In order to better culture umbilical cord stem cells and expand the number of umbilical cord stem cells, the umbilical cord stem cells are placed in a culture bottle after being taken out, and some factors, biological agents and traditional Chinese medicine prescriptions are added artificially to increase the number of cells.

[0003] However, in the prior art, there are at least the following technical problems: the reagent box used for mesenchymal stem cell separation and expansion has poor mixing effect, the reagents placed in the reagent box are prone to precipitation, the mesenchymal stem cells cannot be well contacted with the additives, and the separation and expansion is slow. Therefore, we propose a mesenchymal stem cell separation and expansion kit. UTILITY MODEL CONTENT

[0004] (I) Technical problem solved

[0005] In view of the shortcomings of the prior art, the utility model provides a mesenchymal stem cell separation and expansion kit, which solves the technical problem that the reagent box used for mesenchymal stem cell separation and expansion has poor mixing effect, the reagents placed in the reagent box are prone to precipitation, the mesenchymal stem cells cannot be well contacted with the additives, and the separation and expansion is slow.

[0006] (II) Technical scheme

[0007] To achieve the above purpose, the utility model realizes the following technical scheme:

[0008] A mesenchymal stem cell separation and expansion kit, comprising a reagent box, a cover plate is arranged on the top of the reagent box, a driving part is arranged on the inner bottom of the reagent box, a rectangular block is fixedly connected to the top end of the driving part, a driving assembly is arranged on each side of the rectangular block, an annular plate is fixedly connected to the inner side of the reagent box close to the bottom end, and a plurality of extrusion blocks are fixedly connected to the inner side of the reagent box.

[0009] Preferably, the driving part comprises a speed reducer, the speed reducer is fixedly installed on the inner bottom of the reagent box, the output end of the speed reducer is fixedly connected with a main gear, one side of the main gear is meshedly connected with a slave gear, the middle part of the slave gear is fixedly connected with a rotating shaft, the bottom end of the rotating shaft is rotatably connected with the reagent box, and the top end of the rotating shaft is welded with the rectangular block.

[0010] The technical effect of the further scheme is that the deceleration motor drives the gear to rotate, the gear drives the shaft to rotate, the shaft drives the four connecting rods of the rectangular block to rotate, and the reagent tube and the test tube sleeve rotate around the shaft.

[0011] Preferably, the driving assembly comprises a connecting rod fixedly connected to four sides of the rectangular block, one side of the connecting rod is provided with a hollow rod, one end of the hollow rod is fixedly connected with a rectangular frame, the inside of the rectangular frame is inserted with a test tube sleeve, the inside of the test tube sleeve is placed with a reagent tube, the middle of the test tube sleeve is sleeved with a pressing plate, the spring one is fixedly connected between the pressing plate and the rectangular frame, the top of the annular plate is fixedly connected with a plurality of mounting blocks, the top of the mounting blocks is installed with a plurality of balls, one side of the connecting rod is fixedly connected with a rectangular rod, one side of the hollow rod is provided with a rectangular groove corresponding to the rectangular rod, and the connecting rod and the hollow rod are fixedly connected with the spring.

[0012] The technical effect of the further scheme is that in the process of rotating, the collision block collides with the pressing block inside the reagent box, the force generated by the collision drives the hollow rod to compress the spring, when the collision force disappears, the spring restores, under the action of the restoring force of the spring, the cell tissue fluid in the reagent tube vibrates, so that the cell tissue fluid in the reagent tube is prevented from depositing, at the same time, the bottom of the test tube sleeve collides with the balls above the mounting blocks when the test tube sleeve rotates, under the action of the collision force, the test tube sleeve moves upward and stretches the spring one, when the force disappears, the spring one restores, under the action of the restoring force of the spring one, the pressing plate and the test tube sleeve move downward quickly, so that the cell tissue fluid in the reagent tube is prevented from depositing.

[0013] (Three) beneficial effects

[0014] Compared with the prior art, the beneficial effects of the utility model are:

[0015] 1. The driving member is arranged, and under the cooperation of the deceleration motor, the main gear, the gear and the shaft, the driving assembly can rotate, collide with the pressing block and the ball on the inner wall of the reagent box.

[0016] 2. The driving assembly is arranged, and under the cooperation of the connecting rod, the rectangular rod, the hollow rod, the rectangular groove, the spring, the rectangular frame, the test tube sleeve, the pressing plate, the spring one and the collision block, the cell tissue fluid in the reagent tube can be shaken up and down and left and right by the restoring force of the spring and the spring one, so that the cell tissue fluid is prevented from depositing. DRAWINGS

[0017] The above description is only a summary of the technical scheme of the utility model, in order to more clearly understand the technical means of the utility model, and can be implemented according to the content of the specification, the following is the preferred example of the utility model and is described in detail with the help of the drawings.

[0018] Figure 1 It is the three-dimensional structure schematic diagram in the embodiment of the utility model;

[0019] Figure 2 It is the three-dimensional structure schematic diagram in the embodiment of the utility model; Figure 1 It is the front view structure schematic diagram of the utility model embodiment;

[0020] Figure 3 It is the structure schematic diagram of the driving assembly in the embodiment of the utility model;

[0021] Figure 4 It is the structure schematic diagram of the driving assembly in the embodiment of the utility model; Figure 1 It is the enlarged structure schematic diagram of the place A in the embodiment of the utility model.

[0022] Legend: 1, reagent kit; 101, cover plate; 102, annular plate; 21, driving part; 211, speed reducer motor; 212, main gear; 213, from gear; 214, rotating shaft; 22, rectangular block; 23, driving assembly; 231, connecting rod; 232, rectangular rod; 233, hollow rod; 234, rectangular groove; 235, spring; 236, rectangular frame; 237, test tube cover; 238, extrusion plate; 239, spring one; 230, collision block; 3, reagent tube; 4, mounting block; 41, ball; 43, extrusion block. Specific implementation

[0023] The embodiment of the application provides a mesenchymal stem cell separation and expansion kit, which is driven by the driving part, and is rotated under the cooperation of the speed reducer motor, the main gear, the from gear and the rotating shaft, so that the driving assembly collides with the extrusion block and the ball on the inner wall of the reagent kit. The driving assembly is driven by the connecting rod, the rectangular rod, the hollow rod, the rectangular groove, the spring, the rectangular frame, the test tube cover, the extrusion plate, the spring one and the collision block, so that the reagent tube cell tissue fluid is shaken up and down and left and right by the restoring force of the spring and the spring one, and the cell tissue fluid is effectively prevented from precipitating.

[0024] The technical scheme in the embodiment of the application effectively solves the technical problems that the reagent kit used for mesenchymal stem cell separation and expansion at present has poor mixing effect, the reagent placed in the reagent kit is prone to precipitate, and the mesenchymal stem cells cannot be well contacted with the additive, and the separation and expansion are slow, and the overall idea is as follows:

[0025] For example, Figures 1 to 4The utility model provides a mesenchymal stem cell separation amplification kit, which solves the problems in the prior art, and comprises a kit 1, a cover plate 101 is arranged on the top of the kit 1, a driving part 21 is arranged on the inner bottom of the kit 1, a rectangular block 22 is fixedly connected to the top end of the driving part 21, driving assemblies 23 are arranged on the four sides of the rectangular block 22, a ring plate 102 is fixedly connected to the inner side of the kit 1 close to the bottom end, a plurality of extrusion blocks 43 are fixedly connected to the inner side of the kit 1, the driving part 21 comprises a speed reducer 211, the speed reducer 211 is fixedly installed on the inner bottom of the kit 1, a main gear 212 is fixedly connected to the output end of the speed reducer 211, and a slave gear 213 is meshedly connected to one side of the main gear 212.

[0026] By adopting the above technical scheme, the speed reducer 211 drives the slave gear 213 to rotate through the main gear 212, the slave gear 213 drives the rotating shaft 214 to rotate, the rotating shaft 214 drives the four connecting rods 231 of the rectangular block 22 to rotate, and the reagent tube 3 and the test tube sleeve 237 rotate around the rotating shaft 214 as the center.

[0027] Specifically, the rotating shaft 214 is fixedly connected to the middle part of the slave gear 213, the bottom end of the rotating shaft 214 is rotationally connected to the kit 1, and the top end of the rotating shaft 214 is welded to the rectangular block 22; the driving assembly 23 comprises a connecting rod 231, the connecting rod 231 is fixedly connected to the four sides of the rectangular block 22, a hollow rod 233 is arranged on one side of the connecting rod 231, one end of the hollow rod 233 is fixedly connected to a rectangular frame 236, the test tube sleeve 237 is arranged in the rectangular frame 236, the reagent tube 3 is arranged in the test tube sleeve 237, the extrusion plate 238 is arranged in the middle part of the test tube sleeve 237, the spring 239 is fixedly connected between the extrusion plate 238 and the rectangular frame 236, a plurality of mounting blocks 4 are fixedly connected to the top of the ring plate 102, the ball 41 is arranged on the top end of the mounting block 4, the rectangular rod 232 is fixedly connected to one side of the connecting rod 231, the rectangular groove 234 corresponding to the rectangular rod 232 is formed in one side of the hollow rod 233, and the spring 235 is fixedly connected between the connecting rod 231 and the hollow rod 233.

[0028] By adopting the above technical solution, during the rotation, the collision block 230 will collide with the squeezing block 43 inside the reagent kit 1. The force generated by the collision will drive the hollow rod 233 to compress the spring 235. When the collision force disappears, the spring 235 will return to its original state. Under the action of the spring 235's restoring force, the cell tissue fluid inside the reagent tube 3 will vibrate, thereby preventing the cell tissue fluid inside the reagent tube 3 from precipitating. At the same time, as the test tube sleeve 237 rotates, its bottom end will collide with the ball bearing 41 above the mounting block 4. Under the action of this collision force, the test tube sleeve 237 can move upward and stretch the spring 239. When this force disappears, the spring 239 will return to its original state. Under the action of the spring 239's restoring force, the squeezing plate 238 and the test tube sleeve 237 can move downward quickly, thereby preventing the cell tissue fluid inside the reagent tube 3 from precipitating.

[0029] Working principle: During use, the geared motor 211 drives the driven gear 213 to rotate via the main gear 212. The driven gear 213 drives the rotating shaft 214 to rotate, and the rotating shaft 214 drives the four connecting rods 231 of the rectangular block 22 to rotate. The reagent tube 3 and the test tube sleeve 237 rotate around the rotating shaft 214. During the rotation, the collision block 230 collides with the squeezing block 43 inside the reagent kit 1. The force generated by the collision drives the hollow rod 233 to compress the spring 235. When the collision force disappears, the spring 235 returns to its original state. Under the action of the spring 235's restoring force, the... The cell tissue fluid inside reagent tube 3 vibrates, thus preventing the cell tissue fluid inside reagent tube 3 from settling. At the same time, as the test tube sleeve 237 rotates, its bottom end collides with the ball bearing 41 above the mounting block 4. Under the action of this collision force, the test tube sleeve 237 moves upward and stretches the spring 239. When the force disappears, the spring 239 returns to its original position. Under the action of the restoring force of the spring 239, the squeezing plate 238 and the test tube sleeve 237 can be driven to move downward quickly, thus preventing the cell tissue fluid inside reagent tube 3 from settling.

[0030] Finally, it should be noted that the above embodiments are merely examples for clearly illustrating the present invention and are not intended to limit the implementation. Those skilled in the art can make other variations or modifications based on the above description. It is neither necessary nor possible to exhaustively list all possible implementations here. However, obvious variations or modifications derived therefrom are still within the protection scope of this invention.

Claims

1. A mesenchymal stem cell isolation and expansion kit, comprising kit (1), characterized in that: The reagent kit (1) is provided with a cover plate (101) on the top, and a drive unit (21) is provided at the bottom inside the reagent kit (1). A rectangular block (22) is fixedly connected to the top of the drive unit (21), and a driving component (23) is provided on each of the four sides of the rectangular block (22). An annular plate (102) is fixedly connected to the inner side of the reagent kit (1) near the bottom. Multiple extrusion blocks (43) are fixedly connected to the middle of the inner side of the reagent kit (1).

2. The mesenchymal stem cell isolation and expansion kit as described in claim 1, characterized in that: The drive unit (21) includes a geared motor (211), which is fixedly installed at the bottom of the reagent kit (1). The output end of the geared motor (211) is fixedly connected to a main gear (212), and a driven gear (213) is meshed on one side of the main gear (212).

3. The mesenchymal stem cell isolation and expansion kit as described in claim 2, characterized in that: A rotating shaft (214) is fixedly connected to the middle of the gear (213). The bottom end of the rotating shaft (214) is rotatably connected to the reagent kit (1), and the top end of the rotating shaft (214) is welded to the rectangular block (22).

4. The mesenchymal stem cell isolation and expansion kit as described in claim 1, characterized in that: The driving component (23) includes a connecting rod (231), which is fixedly connected to the four sides of the rectangular block (22). A hollow rod (233) is provided on one side of the connecting rod (231), and a rectangular frame (236) is fixedly connected to one end of the hollow rod (233). A test tube sleeve (237) is inserted inside the rectangular frame (236).

5. The mesenchymal stem cell isolation and expansion kit as described in claim 4, characterized in that: The test tube sleeve (237) contains a reagent tube (3). A squeezing plate (238) is fitted in the middle of the test tube sleeve (237). A spring (239) is fixedly connected between the squeezing plate (238) and the rectangular frame (236). Multiple mounting blocks (4) are fixedly connected to the top of the annular plate (102). Each of the multiple mounting blocks (4) has a ball bearing (41) installed at its top.

6. The mesenchymal stem cell isolation and expansion kit as described in claim 4, characterized in that: A rectangular rod (232) is fixedly connected to one side of the connecting rod (231), and a rectangular groove (234) corresponding to the rectangular rod (232) is opened on one side of the hollow rod (233).

7. The mesenchymal stem cell isolation and expansion kit as described in claim 6, characterized in that: A spring (235) is fixedly connected between the connecting rod (231) and the hollow rod (233).

8. The mesenchymal stem cell isolation and expansion kit as described in claim 5, characterized in that: One end of the rectangular frame (236) is fixedly connected to a collision block (230) corresponding to the extrusion block (43).

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