Cell transfer device

Abstract

The utility model discloses a cell transfer device which comprises a support frame and a transfer pipe, the support frame is provided with at least one mounting part, the transfer pipe is suitable for being mounted on the mounting part, the transfer pipe comprises an outer pipe and an inner cylinder, the inner cylinder is mounted in the outer pipe, the inner cylinder is provided with a filter part, and the filter part is arranged on the inner cylinder. The outer pipe is provided with a liquid outlet, and a solution in the inner cylinder is suitable for entering the outer pipe after being filtered by the filtering part and flowing out from the liquid outlet. According to the cell transfer device disclosed by the embodiment of the utility model, one-time multi-thread transfer can be realized, the next cell transfer work of the same batch can be carried out after some cells are transferred and during a waiting period, the time is saved, the efficiency is improved, meanwhile, cell blocks which cannot be seen clearly by naked eyes can be filtered in the cell transfer process, and the cell transfer efficiency is improved. The block mass problem caused by cell transfer is reduced.

Description

Technical Field

[0001] This invention relates to the field of biotechnology, and in particular to a cell transfer device. Background Technology

[0002] In the process of cell preparation, it is often necessary to transfer cells or replenish cells with fluid. For cell culture plates or cell culture flasks with small transfer volumes and large container diameters, pipettes can usually be used directly.

[0003] For cell culture bags, cell cryopreservation bags, or infusion bags with large transfer volumes and small container openings, auxiliary transfer devices are required. Existing auxiliary transfer devices usually only serve as drainage devices, transferring cell clumps that are not visible to the naked eye from the cell suspension to the cell culture bags, cell cryopreservation bags, or infusion bags, which affects the quality of the final product. In addition, due to the large transfer volume, each cell transfer has a long waiting period, resulting in low efficiency and room for improvement. Utility Model Content

[0004] This invention aims to solve at least one of the technical problems existing in the prior art. To this end, this invention proposes a cell transfer device that can achieve multi-threaded transfer in a single operation, allowing for the transfer of the next cell in the same batch while waiting for some cells to be transferred, saving time and improving efficiency. Simultaneously, it can filter out cell clumps that are not visible to the naked eye during the cell transfer process, reducing quality problems during cell transfer.

[0005] A cell transfer device according to an embodiment of the present invention includes: a support frame and a transfer tube. The support frame is provided with at least one mounting part. The transfer tube is adapted to be installed in the mounting part. The transfer tube includes an outer tube and an inner cylinder. The inner cylinder is installed inside the outer tube. The inner cylinder is provided with a filter part. The outer tube is provided with a liquid outlet. The solution in the inner cylinder is adapted to be filtered by the filter part and then enter the outer tube and flow out from the liquid outlet.

[0006] According to the cell transfer device of this utility model embodiment, when multiple mounting parts are provided, each mounting part can install a transfer tube, and each transfer tube is adapted to be connected to a cell culture bag, cell cryopreservation bag or infusion bag through a liquid outlet, so as to transfer the cell fluid in the transfer tube to the cell culture bag, cell cryopreservation bag or infusion bag, thereby realizing the transfer operation of multiple transfer tubes at the same time. In addition, each transfer tube is provided with a filter part, which can filter out clumps in the cell suspension that are not visible to the naked eye, so as to ensure the quality of the product after transfer.

[0007] According to an embodiment of the cell transfer device of the present invention, the outer tube includes a tube body and a tube cap, the top of the tube body is provided with a tube connector, the tube connector is provided with an external thread, and the tube cap and the tube connector are threadedly connected.

[0008] According to the cell transfer device of this utility model embodiment, the top outer periphery of the inner cylinder is provided with an overlapping portion, the inner cylinder extends into the tube body along the tube joint, and the overlapping portion overlaps the top of the tube joint.

[0009] According to the cell transfer device of this utility model embodiment, the transfer tube is provided with a hanging ear, the mounting part is a mounting hole, the transfer tube is installed in the mounting hole and the hanging ear is supported by the support frame.

[0010] According to the cell transfer device of the present invention, the filter section is constructed as a circular filter sheet, the circular filter sheet is provided with a plurality of filter holes spaced apart along the circumference, each of the filter hole rows is provided with a plurality of filter holes and the plurality of filter holes are arranged at radial intervals along the circular filter sheet.

[0011] According to the cell transfer device of the present invention, there are multiple mounting parts, and the support frame includes multiple side-by-side combined panels, each of the combined panels is provided with at least one mounting part, and the multiple combined panels are detachably connected to each other.

[0012] According to the cell transfer device of this utility model embodiment, in adjacent combined panels, one combined panel is provided with a plug hole and the other combined panel is provided with a connecting rod, the connecting rod being inserted into the plug hole to connect the adjacent combined panels.

[0013] According to an embodiment of the present invention, in the cell transfer device, the support frame includes a support portion, and in the arrangement direction of the plurality of combined panels, the combined panel located at the outer end is connected to the support portion.

[0014] According to an embodiment of the present invention, the cell transfer device includes a support portion comprising a connected vertical support portion and a horizontal support portion, wherein the vertical support portion is connected to the combined panel at one end, and the horizontal support portions corresponding to the combined panels at both ends extend in a direction close to each other.

[0015] Additional aspects and advantages of this invention will be set forth in part in the description which follows, and in part will be obvious from the description, or may be learned by practice of the invention. Attached Figure Description

[0016] The above and / or additional aspects and advantages of this utility model will become apparent and readily understood from the description of the embodiments taken in conjunction with the following drawings, in which:

[0017] Figure 1 This is a schematic diagram of the cell transfer device according to an embodiment of the present invention;

[0018] Figure 2 This is a schematic diagram of the transfer tube of the cell transfer device according to an embodiment of the present invention;

[0019] Figure 3 This is a partial schematic diagram of the transfer tube of the cell transfer device according to an embodiment of the present invention.

[0020] Figure label:

[0021] Cell transfer device 100,

[0022] Transfer tube 1, tube body 11, inner cylinder 12, filter section 121, filter hole row 1211, overlapping section 122, pipe connector 13, hanging ear 14, pipe cap 15, liquid outlet 16, support frame 2, combined panel 21, mounting section 211, insertion hole 212, connecting rod 213, support section 22, vertical support section 221, and horizontal support section 222. Detailed Implementation

[0023] The embodiments of this utility model are described in detail below. Examples of these embodiments are shown in the accompanying drawings, wherein the same or similar reference numerals denote the same or similar elements or elements having the same or similar functions throughout. The embodiments described below with reference to the accompanying drawings are exemplary and are only used to explain this utility model, and should not be construed as limiting this utility model.

[0024] In the description of this utility model, it should be understood that the terms "center," "longitudinal," "transverse," "length," "width," "thickness," "upper," "lower," "front," "rear," "left," "right," "vertical," "horizontal," "top," "bottom," "inner," "outer," "clockwise," "counterclockwise," "axial," "radial," and "circumferential," etc., indicating the orientation or positional relationship shown in the accompanying drawings, are only for the convenience of describing this utility model and simplifying the description, and do not indicate or imply that the device or element referred to must have a specific orientation, or be constructed and operated in a specific orientation, and therefore should not be construed as a limitation of this utility model. Furthermore, features defined with "first" or "second" may explicitly or implicitly include one or more of that feature. In the description of this utility model, unless otherwise stated, "a plurality of" means two or more.

[0025] In the description of this utility model, it should be noted that, unless otherwise explicitly specified and limited, the terms "installation," "connection," and "joining" should be interpreted broadly. For example, they can refer to a fixed connection, a detachable connection, or an integral connection; they can refer to a mechanical connection or an electrical connection; they can refer to a direct connection or an indirect connection through an intermediate medium; and they can refer to the internal connection of two components. Those skilled in the art can understand the specific meaning of the above terms in this utility model based on the specific circumstances.

[0026] The following is for reference. Figures 1-3 The cell transfer device 100 according to an embodiment of the present invention can solve the problems of realizing one-time multi-threaded transfer, allowing the next cell transfer in the same batch to be performed while waiting, saving time and improving efficiency, and filtering out cell clumps that are not visible to the naked eye during the cell transfer process to ensure the quality of cell products.

[0027] like Figure 1-3 As shown, a cell transfer device 100 according to an embodiment of the present invention includes: a support frame 2 and a transfer tube 1.

[0028] The support frame 2 is provided with at least one mounting part 211; the transfer tube 1 is adapted to be installed in the mounting part 211. The transfer tube 1 includes an outer tube and an inner cylinder 12. The inner cylinder 12 is installed in the outer tube. The inner cylinder 12 is provided with a filter part 121. The outer tube is provided with a liquid outlet 16. The solution in the inner cylinder 12 is adapted to be filtered by the filter part 121 and then enter the outer tube and flow out from the liquid outlet 16.

[0029] In practice, the support frame 2 can be provided with one or more mounting parts 211. When the support frame 2 is provided with one mounting part 211, one mounting part 211 is used to install one transfer tube 1. When the support frame 2 is provided with multiple mounting parts 211, each mounting part 211 can be equipped with a transfer tube 1, and each transfer tube 1 is provided with an outlet 16. When the cell fluid in the transfer tube 1 needs to be transferred to a cell culture bag, cell cryopreservation bag or infusion bag, it can flow through the outlet 16 to the corresponding cell culture bag, cell cryopreservation bag or infusion bag, thereby enabling the cell fluid in multiple transfer tubes 1 to be transferred at the same time, improving the efficiency of transfer.

[0030] In addition, the inner cylinder 12 of this utility model embodiment is a filter cylinder. The inner cylinder 12 is provided with a filter section 121. When the cell fluid is put into the transfer tube 1, the cell fluid is actually placed in the inner cylinder 12 and filtered through the filter section 121 of the inner cylinder 12 before entering the interior of the outer tube. This achieves the filtration of cell clumps in the cell suspension that cannot be seen with the naked eye, thereby improving the quality of cell transfer.

[0031] Therefore, the cell transfer device 100 of this utility model embodiment can not only realize multi-threaded operation, but also perform the next cell transfer in the same batch while waiting after some cells have been transferred, saving time and improving efficiency. It can also filter cell fluid, and does not require manual gripping or tilting to collect and transfer cell fluid. It is only necessary to install the transfer tube 1 on the support frame 2 and connect the cell culture bag, cell cryopreservation bag or infusion bag to the outlet 16 of the transfer tube 1.

[0032] In some embodiments, the outer tube includes a tube body 11 and a tube cap 15. The top of the tube body 11 is provided with a tube joint 13, which is provided with external threads. The tube cap 15 and the tube joint 13 are threaded together.

[0033] In actual manufacturing, the pipe connector 13 and the pipe body 11 can be integrated, with the pipe connector 13 located at the end of the pipe body 11. The pipe connector 13 has a hollow cavity, and the pipe body 11 has a receiving cavity. The hollow cavity of the pipe connector 13 and the receiving cavity of the pipe body 11 are connected. When the inner cylinder 12 is inserted into the receiving cavity of the pipe body 11 along the hollow cavity of the pipe connector 13, and the pipe cap 15 is threaded onto the pipe connector 13, the pipe cap 15 can protect the inner cylinder 12 inside the pipe body 11, preventing impurities from falling into the inner cylinder 12, thereby reducing the risk of cell fluid contamination. Furthermore, the threaded connection between the pipe cap 15 and the pipe connector 13 improves the convenience of detachable connection and the reliability of the connection.

[0034] In some embodiments, the top outer periphery of the inner cylinder 12 is provided with an overlapping portion 122, the inner cylinder 12 extends into the pipe body 11 along the pipe joint 13, and the overlapping portion 122 overlaps the top of the pipe joint 13.

[0035] In practice, the inner cylinder 12 may include an inner cylinder body and an overlapping portion 122. That is, the overlapping portion 122 can extend radially along the outer periphery of the top of the inner cylinder body, so that the diameter of the inner cylinder 12 at the overlapping point is larger than the diameter of the inner cylinder body at other positions. In other words, the inner cylinder body can extend into the receiving cavity of the pipe body 11 along the hollow cavity of the pipe joint 13, but the overlapping portion 122 at the top of the inner cylinder body cannot extend into the hollow cavity of the pipe joint 13. Furthermore, the overlapping portion 122 at the top of the inner cylinder body can also overlap the top of the pipe joint 13, thereby fixing the axial position of the inner cylinder 12 relative to the pipe body 11. When the pipe cover 15 is connected to the pipe joint 13, that is, the pipe cover 15 is connected to the pipe joint 13 and the overlapping portion 122 of the inner cylinder 12 at the same time, thereby further ensuring the stability of the connection between the inner cylinder 12 and the outer pipe.

[0036] In some embodiments, the transfer tube 1 is provided with a hanging ear 14, and the mounting part 211 is a mounting hole. The transfer tube 1 is installed in the mounting hole and the hanging ear 14 is supported on the support frame 2.

[0037] Reference Figure 2 As shown, the transfer tube 1 can have two lugs 14, which are arranged facing each other radially along the transfer tube 1. The two lugs 14 have identical structures. When the transfer tube 1 is installed in the mounting hole on the support frame 2, it ensures stability between the transfer tube 1 and the support frame 2. Furthermore, the facing and identical structure of the two lugs 14 improves the uniformity of force distribution after the transfer tube 1 is installed on the support frame 2, preventing tilting or uneven force distribution that could lead to uneven cell fluid distribution and incomplete cell fluid outflow. Alternatively, a ring of integrally formed lugs 14 can be provided around the tube body 11 of the transfer tube 1. When the transfer tube 1 is installed in the mounting part 211, the lugs 14 fit snugly against the support frame 2 for support. Additionally, a certain distance is left between the lugs 14 and the opening of the tube connector 13, facilitating observation of the liquid being poured.

[0038] In some embodiments, the filter section 121 is configured as a circular filter sheet, which has a plurality of filter hole rows 1211 distributed circumferentially, each filter hole row 1211 having a plurality of filter holes and the plurality of filter holes being arranged radially spaced along the circular filter sheet.

[0039] In practice, the circular filter has multiple filter hole rows 1211, which are distributed circumferentially around the center of the circular filter. Each filter hole row 1211 has multiple filter holes. This means that when the cell fluid enters the inner cylinder 12, the cell fluid can be dispersed from the center of the circular filter to different radial directions, and can also flow to different positions along the circumference of the circular filter, thus dispersing the cell fluid more evenly, reducing cell clumping, and improving product quality.

[0040] It should be noted that the bottom of the transfer tube 1 is constructed in an arc shape, and the arc shape is constructed in a funnel shape, so that after the cell fluid flows out from the outlet 16, there is almost no cell fluid residue.

[0041] In some embodiments, there are multiple mounting portions 211, and the support frame 2 includes multiple side-by-side combination panels 21, each combination panel 21 having at least one mounting portion 211, and the multiple combination panels 21 being detachably connected to each other.

[0042] Reference Figure 1 As shown, there are multiple mounting parts 211, and after the multiple combination panels 21 of the support frame 2 are connected, each combination panel 21 can be provided with one mounting part 211, or each combination panel 21 can be provided with two or more mounting parts 211. Then, after multiple combination panels 21 are connected side by side, there are more mounting parts 211. Each mounting part 211 is used to install a transfer tube 1. At this time, multiple transfer tubes 1 can be used to transfer cell fluid at the same time.

[0043] Furthermore, the number of combination panels 21 can be selected according to actual needs. For example, if there are many mounting parts 211 required, the corresponding number of combination panels 21 can be set to be more. Figure 1 As shown, three or four combination panels 21 are set; when the actual required mounting parts 211 are few, two combination panels 21 or one combination panel 21 can be set; of course, under the premise of setting more combination panels 21, the number of transfer tubes 1 can be set less, that is, the number of mounting parts 211 is greater than the number of transfer tubes 1, and the specific design depends on the space size.

[0044] In some embodiments, in adjacent combination panels 21, one combination panel 21 is provided with a plug hole 212 and the other combination panel 21 is provided with a connecting rod 213, the connecting rod 213 being inserted into the plug hole 212 to connect the adjacent combination panels 21.

[0045] Reference Figure 1 As shown, when there are three combination panels 21, the combination panel 21 in the middle is provided with insertion holes 212 and connecting rods 213 on both sides along the arrangement direction of multiple combination panels 21. In the two combination panels 21 at the ends, one combination panel 21 is provided with insertion holes 212 and the other combination panel 21 is provided with connecting rods 213. Then the middle combination panel 21 can be detachably connected to the combination panels 21 at both ends at the same time. Each combination panel 21 is provided with a mounting part 211. The three combination panels 21 can realize the simultaneous installation of three transfer tubes 1, that is, realize multi-threaded cell fluid transfer.

[0046] Specifically, a magnet can be installed inside the insertion hole 212, and a portion of the connecting rod 213 can also be made of magnetic material. When the connecting rod 213 is inserted into the insertion hole 212, the stability and reliability of the insertion can be improved, ensuring the stability of the combined panel 21, that is, maintaining the stability of the transfer tube 1, thereby improving the quality of cell fluid transfer.

[0047] Of course, the number of combination panels 21 in this embodiment of the present invention is not limited to three, but can also be four, five, six, or even more, depending on actual needs; by making each combination panel 21 of the support frame 2 detachable, the structural flexibility of the support frame 2 can be improved, and space can be saved when the number of transfer tubes 1 that need to be set is small.

[0048] In some embodiments, the support frame 2 includes a support portion 22, and in the arrangement direction of the plurality of combination panels 21, the combination panel 21 located at the outer end is connected to the support portion 22.

[0049] The support portion 22, serving as the main body supporting the transfer tube 1, is located at both ends along the arrangement direction of the multiple combined panels 21. This prevents interference between the multiple transfer tubes 1 and the support portion 22 of the support frame 2 when they are connected to corresponding cell culture bags, cell cryopreservation bags, or infusion bags. Furthermore, the location of the support portion 22 at both ends along the arrangement direction of the multiple combined panels 21 improves the stability of the support between the combined panels 21 after assembly. The support portion 22 and the end combined panels 21 can be integrally formed, or they can be detachable. When the support portion 22 and the end combined panels 21 are detachable, it is equivalent to the entire support frame 2 being detachable, saving space during storage. Conversely, when the support portion 22 and the end combined panels 21 are integrally formed, the stability between the end combined panels 21 and the support portion 22 is improved, installation time is saved, and installation efficiency is increased.

[0050] In some embodiments, the support portion 22 includes a connected vertical support portion 221 and a horizontal support portion 222, the vertical support portion 221 is connected to the end of the combined panel 21, and the horizontal support portions 222 corresponding to the combined panels 21 at both ends extend in a direction close to each other.

[0051] In practice, by setting the transverse support portions 222 at both ends to extend toward each other, the entire support frame 2 forms a frame-like structure, which improves the stability of the support and saves space in the direction of the arrangement of multiple combination panels 21. That is, other structures and components can be placed in the area of ​​the support frame 2 outside the direction of the arrangement of multiple combination panels 21 without affecting each other.

[0052] In operation, the cell transfer device 100 of this embodiment first removes the transfer tube 1, removes the tube cap 15, and places the tube body 11 into the mounting hole of the support frame 2. The tubing of the cell culture bag, cell cryopreservation bag, or infusion bag is then connected to the outlet 16 at the bottom of the tube body 11. The cell solution to be transferred is poured into the inner cylinder 12. After being filtered by the filter section 121, the cell solution flows from the outlet 16 through the tubing into the cell culture bag, cell cryopreservation bag, or infusion bag. When multi-threaded operation is required, the support frame 2 can be combined with several combination panels 21 to hold multiple transfer tubes 1 for cell transfer.

[0053] In the description of this specification, the references to terms such as "one embodiment," "some embodiments," "illustrative embodiment," "example," "specific example," or "some examples," etc., indicate that a specific feature, structure, material, or characteristic described in connection with that embodiment or example is included in at least one embodiment or example of the present invention. In this specification, the illustrative expressions of the above terms do not necessarily refer to the same embodiment or example. Furthermore, the specific features, structures, materials, or characteristics described may be combined in any suitable manner in one or more embodiments or examples.

[0054] Although embodiments of the present invention have been shown and described, those skilled in the art will understand that various changes, modifications, substitutions and alterations can be made to these embodiments without departing from the principles and spirit of the present invention, the scope of which is defined by the claims and their equivalents.

Claims

1. A cell transfer device, characterized in that, include: The support frame is provided with at least one mounting part; A transfer tube is adapted to be installed in the mounting part. The transfer tube includes an outer tube and an inner cylinder. The inner cylinder is installed inside the outer tube. The inner cylinder is provided with a filter section. The outer tube is provided with a liquid outlet. The solution in the inner cylinder is adapted to be filtered by the filter section and then enter the outer tube and flow out from the liquid outlet.

2. The cell transfer device according to claim 1, characterized in that, The outer tube includes a tube body and a tube cap. The top of the tube body is provided with a tube joint, and the tube joint is provided with external threads. The tube cap and the tube joint are threaded together.

3. The cell transfer device according to claim 2, characterized in that, The inner cylinder has an overlapping portion on its top outer periphery. The inner cylinder extends into the pipe body along the pipe joint, and the overlapping portion overlaps the top of the pipe joint.

4. The cell transfer device according to claim 1, characterized in that, The transfer tube is provided with a hanging ear, the mounting part is a mounting hole, the transfer tube is installed in the mounting hole and the hanging ear is supported by the support frame.

5. The cell transfer device according to claim 1, characterized in that, The filter section is constructed as a circular filter sheet, which has multiple rows of filter holes spaced apart circumferentially. Each row of filter holes has multiple filter holes, and the multiple filter holes are arranged at radial intervals along the circular filter sheet.

6. The cell transfer device according to claim 1, characterized in that, The mounting portion is multiple, and the support frame includes multiple side-by-side combination panels. Each combination panel is provided with at least one mounting portion, and the multiple combination panels are detachably connected to each other.

7. The cell transfer device according to claim 6, characterized in that, In the adjacent combination panels, one combination panel is provided with a plug hole and the other combination panel is provided with a connecting rod, the connecting rod being inserted into the plug hole to connect the adjacent combination panels.

8. The cell transfer device according to claim 7, characterized in that, The support frame includes a support portion, and in the arrangement direction of the plurality of combined panels, the combined panel located at the outer end is connected to the support portion.

9. The cell transfer device according to claim 8, characterized in that, The support includes a connected vertical support and a horizontal support, the vertical support being connected to the combined panel at one end, and the horizontal support corresponding to the combined panels at both ends extending in a direction close to each other.

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