Silk fibroin dissolving and dialyzing device

Abstract

The utility model relates to a silk fibroin dissolving and dialyzing device which comprises a containing mechanism, the containing mechanism comprises a first tank body and a second tank body, a stirring space and a dialyzing space are defined by the first tank body and the second tank body respectively, the stirring space and the dialyzing space are partially communicated, and a dialyzing diaphragm is arranged at the communication position of the stirring space and the dialyzing space; the first tank body is respectively provided with a feed port and a discharge port, and the second tank body is respectively provided with a first water inlet and a water outlet; and the filament dissolving mechanism is arranged on the first tank body and comprises a stirring assembly, a heating assembly, a chassis and a first driving assembly. Through the arrangement, a silk fibroin solution in the stirring space and a dialysis solution in the dialysis space can be completely separated or separated through the dialysis diaphragm, and the dialysis process is detected through the conductivity monitor. And the dissolving process and the dialysis process can be sequentially and automatically carried out, so that the dialysis efficiency is improved.

Description

TECHNICAL FIELD

[0001] The utility model relates to dialysis field especially is a kind of silk fibroin dissolving dialysis device. BACKGROUND

[0002] Silk fibroin is a kind of protein-based biological macromolecule extracted from natural cocoon, with excellent mechanical properties, biocompatibility and biodegradability, and is a kind of biological medical material with broad application prospect. In recent years, high-end medical devices such as bone nail, artificial brain membrane, sustained-release microneedle, cardiovascular stent and artificial bone prepared from silk fibroin are widely used in orthopedics, sports department, cardiovascular department, brain surgery, plastic medical beauty and other fields. Generally speaking, silk fibroin is mainly extracted from natural cocoon by solution method. The specific steps include: (1) degumming with alkali to obtain degummed silk; (2) dissolving degummed silk with high-concentration salt solution (such as lithium bromide); (3) dialysis purification.

[0003] Although a relatively ideal silk fibroin solution can be obtained by this method, the solution needs to be transferred to a dialysis bag for dialysis purification after dissolving the degummed silk solution, which results in large loss during the transfer process and low dialysis efficiency due to small capacity, easy leakage of the dialysis bag and frequent manual replacement of dialysis solution during dialysis. UTILITY MODEL CONTENT

[0004] Therefore, the utility model solves the technical problems in the prior art that the solution needs to be transferred to a dialysis bag for dialysis purification after dissolving the degummed silk solution, which results in large loss during the transfer process and low dialysis efficiency due to small capacity, easy leakage of the dialysis bag and frequent manual replacement of dialysis solution during dialysis, and provides a silk fibroin dissolving dialysis device.

[0005] To solve the above technical problems, the utility model provides a silk fibroin dissolving dialysis device, which comprises:

[0006] The accommodating mechanism comprises a first tank body and a second tank body, the first tank body and the second tank body respectively enclose a stirring space and a dialysis space, the stirring space and the dialysis space are at least partially communicated, and a dialysis diaphragm is arranged at the communication position, the first tank body is respectively provided with a feeding port and a discharging port, and the second tank body is respectively provided with a first water inlet and a water outlet.

[0007] The silk dissolving mechanism is arranged in the first tank body and comprises a stirring assembly, a heating assembly, a bottom disc and a first driving assembly, the stirring assembly is arranged in the first tank body, the heating assembly is arranged at the bottom of the first tank body and is used for heating the solution in the stirring space, the peripheral side of the bottom disc is sealingly connected to the inner wall of the first tank body, the output end of the first driving assembly is connected to the bottom disc, and the bottom disc is movably connected to the first tank body along a first direction.

[0008] a detection assembly disposed in the second tank body and configured to detect the conductivity of the solution in the dialysis space.

[0009] In an embodiment of the present application, the stirring assembly comprises a driving motor and a stirring paddle, the stirring paddle is connected to the output end of the driving motor, the driving motor can adjust the stirring rate, and the stirring paddle extends into the stirring space.

[0010] In an embodiment of the present application, the stirring assembly is movably disposed in the first tank body along a first direction.

[0011] In an embodiment of the present application, the height of the top of the base plate relative to the ground can be less than or greater than the height of the bottom of the dialysis membrane relative to the ground.

[0012] In an embodiment of the present application, the detection assembly is a conductivity monitor, and the sensing unit of the conductivity monitor extends to a lower region in the dialysis space.

[0013] In an embodiment of the present application, the stirring space is further provided with a second water inlet, and the second water inlet is connected with a water inlet pipeline.

[0014] In an embodiment of the present application, a temperature sensor is disposed in the stirring space.

[0015] In an embodiment of the present application, the first driving assembly is a linear driving member, and the output end of the linear driving member is connected to the base plate.

[0016] In an embodiment of the present application, the stirring assembly is disposed on the base plate.

[0017] In an embodiment of the present application, a second driving assembly is further provided, the second driving assembly is configured to drive the stirring assembly to move up and down, the stirring assembly has at least two groups of paddles, and the paddles are disposed at different heights of the stirring assembly, respectively.

[0018] Compared with the prior art, the above technical solution of the present application has the following advantages:

[0019] The silk fibroin dissolving and dialysis device can completely separate the silk fibroin solution in the stirring space from the dialysis solution in the dialysis space or separate them through the dialysis membrane, and can realize efficient dialysis through the independent space of the accommodating mechanism and the dialysis membrane; the silk dissolving mechanism has multiple components that cooperate with each other, and can flexibly adjust the stirring and heating to improve the dissolving quality; the conductivity monitor is used to detect the dialysis process. The dissolving process and the dialysis process can be automatically performed in sequence, and the dialysis efficiency is improved. BRIEF DESCRIPTION OF DRAWINGS

[0020] In order to make the content of the utility model more easily be clearly understood, the following according to the specific embodiment of the utility model and combining with the drawings, the utility model is further explained in detail, wherein

[0021] Fig. 1 It is the structure diagram of dialysis device of the utility model;

[0022] Fig. 2 It is the structure diagram of second drive assembly of the utility model.

[0023] The description of the drawing of the specification is explained: 1, first tank body;2, second tank body;3, bottom disc;4, elastic member;5, stirring assembly;6, feed inlet;7, second water inlet;8, discharge port;9, dialysis diaphragm;10, drive motor;11, support;12, conductivity monitor;13, water outlet;14, first drive assembly;15, stopper;16, first water inlet;17, rope winding machine;18, guide wheel;19, rope body;20, stand;21, sliding frame. Specific embodiment

[0024] The utility model is further explained in combination with the drawings and specific embodiment, so that the person skilled in the art can better understand the utility model and can be implemented, but the embodiment is not as the limitation of the utility model.

[0025] Embodiment

[0026] Refer to Figs. 1-2 The utility model discloses a silk fibroin dissolving dialysis device, which comprises:

[0027] The accommodating mechanism comprises a first tank body 1 and a second tank body 2, the first tank body 1 and the second tank body 2 enclose a stirring space and a dialysis space respectively, the stirring space and the dialysis space are at least partially communicated, and a dialysis diaphragm 9 is arranged at the communication position; in the embodiment, the first tank body 1 and the second tank body 2 share a side wall on the side close to each other, and a communication port is formed in the side wall; the dialysis diaphragm 9 is sealingly arranged in the communication port, so that the solution in the stirring space needs to pass through the dialysis diaphragm 9 to enter the dialysis space; the first tank body 1 is provided with a feed inlet 6 and a discharge port 8; and the second tank body 2 is provided with a first water inlet 16 and a water outlet 13.

[0028] The silk dissolving mechanism is arranged in the first tank body 1 and comprises a stirring assembly 5, a heating assembly, a bottom disc 3 and a first drive assembly 14; the stirring assembly 5 is arranged in the first tank body 1; the heating assembly is arranged in the first tank body 1 and is used for heating the stirring space; the peripheral side of the bottom disc 3 is sealingly connected to the inner wall of the first tank body 1; the output end of the first drive assembly 14 is connected to the bottom disc 3; and the bottom disc 3 is movably connected to the first tank body 1 along a first direction.

[0029] a detection assembly arranged in the second tank body 2 and used for detecting the solution conductivity in the dialysis space.

[0030] The silk fibroin dissolving and dialysis device has the advantages that the first tank body 1 and the second tank body 2 are respectively used to construct a stirring space and a dialysis space, and provide corresponding places for the dissolution of degummed silk and the dialysis of silk fibroin solution. The dialysis diaphragm 9 is arranged at the communication position of the stirring space and the dialysis space, and plays a key role in isolation and selective permeation, so that selective exchange of silk fibroin solution can be realized while different operations are performed in different spaces. The stirring assembly 5 accelerates the mixing of silk and lithium bromide solution through stirring, and improves the dissolution efficiency; the heating assembly can heat the lithium bromide solution, and adjust the temperature according to the needs of silk dissolution, and promote the progress of the dissolution reaction. The bottom disc 3 is sealingly connected to the inner wall of the first tank body 1, which not only ensures the sealing property of the stirring space, but also can move along the first direction under the action of the first driving assembly 14, so that the top end of the liquid surface of the silk fibroin solution is below the bottom end of the dialysis diaphragm 9 during stirring, heating and dissolution, and the silk that is not completely dissolved is prevented from being mixed with the lithium bromide solution through the dialysis diaphragm 9. After complete dissolution, the bottom disc 3 is lifted, the height of the bottom of the bottom disc 3 is not lower than the bottom end of the dialysis diaphragm 9, and the height of the bottom of the bottom disc 3 is not higher than the top end of the dialysis diaphragm 9, so that the silk fibroin solution in the stirring space is dialyzed through the dialysis diaphragm 9, and the lithium bromide component is removed into the dialysis space. The detection assembly is used to detect the conductivity of the solution in the dialysis space. When the concentration of lithium bromide is too high, the conductivity of the solution in the dialysis space is also increased, the water outlet 13 of the second tank body 2 is opened to discharge the lithium bromide solution with too high concentration, and water is added through the water inlet, so that the concentration of lithium bromide in the water solution in the dialysis space is kept at a low state, thereby ensuring the dialysis effect. When the conductivity of the lithium bromide solution in the dialysis space is lower than the set value within the set time, it is determined that the dialysis is completed, and the prepared silk fibroin solution is taken out through the discharge port 8.

[0031] Referring to Fig. 1As shown, the stirring assembly 5 includes a driving motor 10 and a stirring paddle connected to the output end of the driving motor 10, which extends into the stirring space. The driving motor 10 starts to operate after being connected to the power supply. The motor output shaft transmits the rotating power to the stirring paddle. The stirring paddle rotates synchronously with the rotation of the driving motor 10 output shaft, and the paddle blade moves in the solution in the stirring space. When the paddle blade rotates, it exerts force on the surrounding solution, causing the solution to flow. Specifically, the paddle blade pushes the surrounding solution to flow in the radial and axial directions. The radial flow causes the solution to spread from the center of the stirring paddle to the tank wall, while the axial flow causes the solution to circulate in the up-down direction of the stirring space. The degummed silk raw materials and lithium bromide solution in the stirring space are continuously mixed, and the degummed silk that was originally gathered together is dispersed into the lithium bromide solution, increasing the contact area between the silk and the solution and thus accelerating the dissolution process of the silk. As the stirring continues, the solution concentration in the entire stirring space gradually becomes uniform, improving the dissolution efficiency. In this embodiment, lithium bromide and water are first added in the appropriate ratio through the feed inlet 6 and the second water inlet 7, respectively, and are stirred and dissolved at an appropriate temperature to form a uniform lithium bromide solution. Then, the degummed silk is added to the first tank body 1 and mixed with the lithium bromide solution for stirring and dissolution.

[0032] The stirring assembly 5 is movably arranged in the first tank body 1 in the first direction. In this embodiment, the first direction is the up-down direction. In the initial stage of the degummed silk dissolution, the silk is just added to the stirring space and will gather in the bottom area. At this time, the stirring assembly 5 is moved to a position close to the raw material gathering area by the driving mechanism. The driving mechanism moves the stirring assembly 5 downward to approach the raw material gathering area. At different stages of the dissolution process, the operator can adjust the position of the stirring assembly 5 through the control system according to the actual state of the solution and the stirring effect. When it is found that the solution in the upper part of the stirring space is not mixed sufficiently, the driving mechanism moves the stirring assembly 5 upward to more effectively stir the upper solution. The stirring assembly 5 can move up and down during the stirring process to improve the fluidity and stirring effect.

[0033] The height of the top of the bottom plate 3 relative to the ground can be less than or greater than the height of the bottom of the dialysis membrane 9 relative to the ground. When the bottom plate 3 is at the low point, the top end of the silk fibroin solution is below the dialysis membrane 9, avoiding the communication between the first tank body 1 and the second tank body 2, and ensuring sufficient stirring before dialysis. When the bottom plate 3 is at the high point, the silk fibroin solution can remove the lithium bromide component through the dialysis membrane 9.

[0034] The detection component is a conductivity monitor 12, and the sensing unit of the conductivity monitor 12 extends to the lower region in the dialysis space. The sensing unit of the conductivity monitor 12 is always in the lower region in the dialysis space and keeps in contact with the solution in the dialysis space. When the ions in the solution move directionally under the action of an electric field, the electrodes of the sensing unit will sense the flow of the ions, thereby generating an electric signal proportional to the conductivity of the solution. The ion concentration in the solution in the dialysis space is low, and the value displayed by the conductivity monitor 12 is relatively small. As the dialysis process proceeds, the lithium bromide component continuously enters the dialysis space from the stirring space through the dialysis membrane 9, the ion concentration in the solution gradually increases, and the conductivity also rises.

[0035] The stirring space is also provided with a second water inlet 7, and the water inlet is connected with a water inlet pipeline. The water inlet is independently controlled through the second water inlet 7, thereby improving the convenience of water injection of the first tank body 1.

[0036] In the embodiment, the first water inlet 16, the second water inlet 7, the water outlet 13, the material inlet 6 and the material outlet 8 are respectively provided with valves or gates.

[0037] The stirring space is provided with a temperature sensor. The temperature sensor is used for detecting the heating temperature. In the embodiment, the target value of the heating temperature is 60-80℃, and preferably 70℃. When the temperature is too low, the system will control the heating component to increase the heating power and increase the temperature of the stirring space. When the temperature is too high, the system may take measures such as reducing the heating power or starting the cooling device to restore the temperature to the appropriate range.

[0038] The first driving component 14 is a linear driving member, and the output end of the linear driving member is connected with the bottom disc 3. The linear driving member is any one of an electric push rod, an air cylinder and a hydraulic cylinder. The position of the bottom disc 3 in the stirring space can be flexibly adjusted, the stirring effect is optimized, and the material exchange state between the stirring space and the dialysis space can be adjusted according to the needs, thereby meeting the process requirements in different stages of the silk fibroin dialysis process. The stirring component 5 is arranged on the bottom disc 3, so that the stirring component 5 moves up and down with the bottom disc 3.

[0039] Reference Fig. 2The second driving assembly is used to drive the stirring assembly 5 to move up and down. The second driving assembly is used to drive the stirring assembly 5 to move up and down independently. The weight of the bottom plate 3 is reduced, so that the sealing effect of the bottom plate 3 is guaranteed. In the embodiment, the second driving assembly comprises a rope winding machine 17, a guide wheel 18, a support 11, a driving motor 10 and a rope 19. The rope winding machine 17 is similar to a winding device such as an electric hoist. The rope 19 is wound on the rope winding disc of the rope winding machine 17, so that the length of the rope 19 is changed. The rope 19 changes the extension direction through the support 11 and the guide wheel 18, so that the rope 19 can drive the stirring assembly 5 to move in the up-down direction. An elastic member 4 is arranged between the bottom of the first tank body 1 and the stirring assembly 5. The elastic member 4 is an elastic telescopic rod. The telescopic rod of the first driving assembly 14 is sealed through the bottom of the first tank body 1 and is connected to the bottom plate 3. The bearing of the bottom plate 3 is provided with a sealing ring on the inner wall of the first tank body 1 to form a piston seal. The telescopic rod of the first driving assembly 14 and the first tank body 1 are sealed by a packing ring, soft packing or hard packing. A stand 20 is further provided. The stand 20 is movably connected with a sliding frame 21. The end of the rope 19 is connected to the sliding frame 21. The driving motor 10 and the stirring assembly 5 are arranged on the sliding frame 21. The stand 20 is further provided with a fixing frame below the sliding frame 21. The fixing frame is provided with a shaft sleeve sleeved on the stirring shaft, so as to improve the stability of the up-down movement.

[0040] A stopper 25 is arranged at the bottom of the first tank body 1, which is used to stop and limit the lower limit position of the bottom plate 3.

[0041] Obviously, the above embodiment is only an example for clearly illustrating, and is not a limitation to the embodiments. Other different forms of changes or variations can be made by those skilled in the art on the basis of the above description. All the embodiments do not need to be exhausted, and the obvious changes or variations derived therefrom are still within the protection scope of the utility model.

Claims

1. A silk fibroin dissolving dialysis device, characterized by, The application relates to a dialysis device. The device comprises a containing mechanism, a dissolving mechanism and a detecting mechanism. The containing mechanism comprises a first tank body and a second tank body, which enclose a stirring space and a dialysis space respectively, the stirring space and the dialysis space are at least partially communicated, and a dialysis diaphragm is arranged at the communication position; the first tank body is provided with a feeding port and a discharging port respectively; the second tank body is provided with a first water inlet and a water outlet respectively. The dissolving mechanism is arranged in the first tank body and comprises a stirring assembly, a heating assembly, a bottom disc and a first driving assembly; the stirring assembly is arranged in the first tank body; the heating assembly is arranged at the bottom of the first tank body and is used for heating the solution in the stirring space; the bottom disc is sealingly connected to the inner wall of the first tank body; the output end of the first driving assembly is connected to the bottom disc; and the bottom disc is movably connected to the first tank body along a first direction.

2. The silk fibroin dissolving and dialyzing device according to claim 1, characterized in that: The detecting mechanism is arranged in the second tank body and is used for detecting the electric conductivity of the solution in the dialysis space.

3. The silk fibroin dissolving and dialyzing device according to claim 1, characterized in that: The stirring assembly comprises a driving motor and a stirring paddle; the stirring paddle is connected to the output end of the driving motor; and the stirring paddle extends into the stirring space.

4. The silk fibroin dissolving and dialyzing device according to claim 1, characterized in that: The stirring assembly is movably arranged in the first tank body along the first direction.

5. The silk fibroin dissolving and dialyzing device according to claim 1, characterized in that: The height of the top of the bottom disc relative to the ground is less than or greater than the height of the bottom of the dialysis diaphragm relative to the ground.

6. The silk fibroin dissolving and dialyzing device according to claim 1, characterized in that: The detecting mechanism is an electric conductivity monitor; the sensing unit of the electric conductivity monitor extends to a lower region in the dialysis space and is immersed in the dialysis solution.

7. The silk fibroin dissolving and dialyzing device according to claim 1, characterized in that: The stirring space is further provided with a second water inlet; and the second water inlet is externally connected with a water inlet pipeline.

8. The silk fibroin dissolving and dialyzing device according to claim 1, characterized in that: A temperature sensor is arranged in the stirring space.

9. The silk fibroin dissolving and dialyzing device according to claim 1, characterized in that: The first driving assembly is a linear driving element; the output end of the linear driving element is connected to the bottom disc.

10. The silk fibroin dissolving and dialyzing device according to claim 1, characterized in that: The stirring assembly is arranged in the bottom disc. The device further comprises a second driving assembly which is used for driving the stirring assembly to move up and down; the stirring assembly has at least two groups of paddles which are arranged at different heights of the stirring assembly respectively.

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