Rotation head for measuring and analyzing plant xylem embolism fragility curve

Abstract

The utility model relates to the technical field of centrifugal machines, in particular to a rotating head for measuring and analyzing plant xylem embolism fragility curves, which comprises a rotating head body provided with a containing cavity. A plurality of cuvettes are arranged in the containing cavity, the number of the cuvettes is even, each cuvette comprises two vertically arranged bottom plates, the tail ends of the two bottom plates are inwards, vertically and fixedly connected with baffles, the two sides of the two bottom plates are fixedly connected with side plates, and the cuvettes form two vertical grooves through the bottom plates, the side plates and the baffles; the two tail ends of the plant stem section are placed in the corresponding cuvettes; a mounting assembly for fixing a plurality of plant stem sections is mounted in the accommodating cavity; according to the utility model, the cuvettes are arranged in the accommodating cavity, so that the two ends of the plant stem section can be positioned in the corresponding cuvettes, and then the plant stem section is fixed through the mounting assembly, namely, the equipment can accommodate a plurality of plant stem section samples.

Description

TECHNICAL FIELD

[0001] The utility model relates to centrifuge technical field, concretely relates to a kind of head of measuring analysis plant xylem embolism fragile curve. BACKGROUND

[0002] In order to better understand the relationship between plant xylem embolism and plant drought tolerance, the concept of embolism vulnerability is introduced, and the vulnerability curve of embolism is constructed to describe the vulnerability of plant xylem embolism. At present, there are many methods for measuring xylem embolism vulnerability. Among them, the plant xylem centrifugal embolism induction method is one of the methods for measuring embolism vulnerability curve. This method can establish embolism vulnerability curve on xylem segments of different lengths, and is not easy to produce "open conduit false appearance". In the above method, centrifuge is needed, and the plant stem to be detected needs to be placed on the centrifuge head during use, and the plant stem is immersed in water. In the patent number: "201610898775.4", the name is: "a centrifuge method and equipment for measuring plant water conductivity", the plant stem is limited, and when multiple plant stems need to be centrifuged, the problem of low efficiency exists. Therefore, our company provides a kind of head of measuring analysis plant xylem embolism fragile curve according to the state of plant xylem embolism vulnerability determination and the centrifugation of plant stem sample. UTILITY MODEL CONTENT

[0003] In view of the deficiencies of the prior art, the utility model provides a kind of head of measuring analysis plant xylem embolism fragile curve, with the advantage of being able to centrifuge multiple samples simultaneously, solving the problems in the background art.

[0004] The head of measuring analysis plant xylem embolism fragile curve of the utility model, comprising a head body, a containing cavity is provided on the head body;

[0005] The inside of the containing cavity is provided with a plurality of cuvettes, the number of cuvettes is double, and the cuvettes include two vertically arranged bottom plates, the ends of the two bottom plates are vertically fixedly connected with baffles inward, the two sides of the two bottom plates are fixedly connected with side plates, and the cuvettes form two vertical grooves through the bottom plates, side plates and baffles;

[0006] The two ends of the plant stem are placed in the corresponding cuvettes;

[0007] The inside of the containing cavity is provided with a plurality of plant stems.

[0008] In some embodiments, the installation assembly comprises a plurality of support screws, which are threadedly installed on the upper surface of the rotating head body and are not located on the same diameter of the cuvette, and a passage a for the plant stem segments is formed between the support screws; a corresponding number of pressing plates are sleeved on the support screws corresponding to the plant stem segments, and the plant stem segments and the pressing plates are sequentially and repeatedly placed inside the accommodating cavity.

[0009] In some embodiments, the rotating head body comprises a rotating head body, an annular side wall, and a rotating head cover plate, the annular side wall is fixedly installed on the edge of the upper surface of the rotating head body, and the rotating head cover plate is detachably installed on the top of the annular side wall, and the rotating head body, the annular side wall, and the rotating head cover plate constitute an accommodating cavity for placing the plant stem segments.

[0010] In some embodiments, the upper surface of the rotating head body is provided with a plurality of installation grooves for installing cuvettes.

[0011] In some embodiments, the top of the annular side wall is provided with a fitting groove, the rotating head cover plate is installed inside the fitting groove, and the distance between the rotating head cover plate and the inner wall bottom of the taper hole groove is equal to the height of the cuvette.

[0012] In some embodiments, the upper surface of the rotating head cover plate is provided with a through hole corresponding to the support screw, the support screw extends outward through the through hole on the rotating head cover plate, and the rotating head cover plate is fixed on the support screw through a nut.

[0013] In some embodiments, the bottom of the rotating head body is provided with a taper hole groove for connecting with the centrifuge driving mechanism, the top center of the rotating head body is provided with a locking groove in communication with the taper hole groove, and the locking groove is provided with a locking component for locking the centrifuge driving mechanism.

[0014] In some embodiments, the locking component comprises a hexagonal sleeve installed in the taper hole groove through a screw, a butt joint hole for installing the centrifuge driving mechanism is formed in the center of the hexagonal sleeve, an internal hexagonal bolt is installed in the butt joint hole, and the internal hexagonal bolt is threadedly inserted on the centrifuge driving mechanism through the butt joint hole to lock the centrifuge driving mechanism.

[0015] In some embodiments, the top center of the hexagonal sleeve is provided with an installation groove for installing the head of the internal hexagonal bolt, and an elastic check ring, a disc washer, and an aluminum gasket are sequentially installed between the head of the internal hexagonal bolt and the inner wall of the installation groove.

[0016] In some embodiments, a dismounting ejection block is further included, which comprises a driving part, a force receiving part and an ejection part, the ejection part is matched with the butt joint hole of the hexagonal sleeve, and the outer side of the driving part is provided with a thread matched with the inner wall of the mounting groove of the hexagonal sleeve, the dismounting ejection block is connected with the mounting groove of the hexagonal sleeve through the thread of the driving part, so that the ejection part in the butt joint hole extrudes the centrifuge driving mechanism in the butt joint hole.

[0017] Compared with the prior art, the utility model has the advantages that:

[0018] The utility model discloses a plant stem section can be fixed through the installation component, and the equipment can accommodate multiple plant stem section samples. BRIEF DESCRIPTION OF DRAWINGS

[0019] The accompanying drawings, which are included to provide a further understanding of the application and are incorporated in and constitute a part of this application, illustrate embodiments of the application and serve to explain the principles of the application, and do not limit the application. In the drawings:

[0020] Figure 1 It is the front view structure schematic diagram of the embodiment one of the utility model;

[0021] Figure 2 It is the top view structure schematic diagram of the embodiment one of the utility model;

[0022] Figure 3 It is the front view structure schematic diagram of the utility model cuvette;

[0023] Figure 4 It is the front view structure schematic diagram of the embodiment two of the utility model;

[0024] Figure 5 It is Figure 4 It is the enlarged structure schematic diagram of the place A in the middle;

[0025] Figure 6 It is the structure schematic diagram of the dismounting ejection block in the embodiment two;

[0026] Figure 7 It is the structure schematic diagram of the ejection block in the embodiment two.

[0027] In the drawing: 1, rotating head body; 101, rotating head body; 102, annular side wall; 103, rotating head cover plate; 104, mounting groove; 105, fitting groove; 106, taper hole groove; 107, locking groove;

[0028] 2, accommodating cavity; 3, plant stem section;

[0029] 4. cuvette; 41. base plate; 42. baffle; 43. side plate; 44. groove;

[0030] 5. mounting assembly; 51. support screw; 52. nut; 53. pressing plate;

[0031] 6. lifting ring; 7. centrifuge driving mechanism;

[0032] 8. locking component; 81. hexagonal sleeve; 82. inner hexagonal bolt; 83. elastic check ring; 84. disc washer; 85. aluminum gasket; 86. butt joint hole;

[0033] 9. dismounting ejection block; 91. force receiving part; 92. driving part; 93. ejection part. DETAILED DESCRIPTION

[0034] In the following, a plurality of embodiments of the present application will be disclosed with reference to the drawings. For the purpose of clear illustration, many details of the embodiments will be described in the following description. However, it should be understood that these details of the embodiments should not be used to limit the present application. That is, in some embodiments of the present application, these details of the embodiments are not necessary. In addition, for the purpose of simplifying the drawings, some conventional structures and components will be shown in the drawings in a simple schematic manner.

[0035] In addition, in the present application, the description such as "first", "second", etc. is only for the purpose of description, and does not particularly mean the order or sequence, nor is used to limit the present application. It is merely used to distinguish the components or operations described by the same technical terms, and cannot be understood as indicating or implying the relative importance of the indicated technical features or implicitly indicating the number of the indicated technical features. Therefore, the features limited by "first" and "second" can explicitly or implicitly include at least one of the features. In addition, the technical solutions of each embodiment can be combined with each other, but it must be based on the realization of a person skilled in the art. When the combination of technical solutions contradicts each other or cannot be realized, it should be considered that the combination of technical solutions does not exist, nor is within the protection scope required by the present application.

[0036] Embodiment one:

[0037] Please refer to Figure 1 - Figure 3 The present application discloses a measuring and analyzing plant xylem embolism vulnerability curve rotor, which comprises a rotor body 1, and a containing cavity 2 is arranged on the rotor body 1.

[0038] The inside of the accommodating cavity 2 is provided with a plurality of cuvettes 4, the number of cuvettes 4 is double, and the cuvettes 4 include two vertically arranged bottom plates 41, the ends of the two bottom plates 41 are vertically and inwardly fixedly connected with baffles 42, and the two sides of the two bottom plates 41 are fixedly connected with side plates 43, and the cuvettes 4 form two vertical grooves 44 through the bottom plates 41, the side plates 43 and the baffles 42.

[0039] The two ends of the plant stem segments 3 are placed in the corresponding cuvettes 4;

[0040] The inside of the accommodating cavity 2 is provided with a plurality of cuvettes 4, the number of cuvettes 4 is double, and the cuvettes 4 include two vertically arranged bottom plates 41, the ends of the two bottom plates 41 are vertically and inwardly fixedly connected with baffles 42, and the two sides of the two bottom plates 41 are fixedly connected with side plates 43, and the cuvettes 4 form two vertical grooves 44 through the bottom plates 41, the side plates 43 and the baffles 42.

[0041] The installation assembly 5 includes a plurality of support screws 51, the plurality of support screws 51 are threadedly installed on the upper surface of the rotating head body 101, and the support screws 51 are not located on the same diameter of the rotating head body 1 as the cuvettes 4, and the support screws 51 form passages a for the plant stem segments 3 to pass through; a plurality of pressing plates 53 can be sleeved on the support screws 51 corresponding to the number of plant stem segments 3, and the plant stem segments 3 and the pressing plates 53 are sequentially and repeatedly placed in the accommodating cavity 2.

[0042] The rotating head body 1 includes a rotating head body 101, an annular side wall 102 and a rotating head cover plate 103, the annular side wall 102 is fixedly installed on the edge of the upper surface of the rotating head body 101, and the rotating head cover plate 103 is detachably installed on the top of the annular side wall 102, and the rotating head body 101, the annular side wall 102 and the rotating head cover plate 103 form the accommodating cavity 2 for placing the plant stem segments 3.

[0043] The upper surface of the rotating head body 101 is provided with a plurality of installation grooves 104 for installing the cuvettes 4.

[0044] The top of the annular side wall 102 is provided with a fitting groove 105, and the rotating head cover plate 103 is installed in the fitting groove 105, and the distance between the rotating head cover plate 103 and the inner wall bottom of the taper hole groove 106 is equal to the height of the cuvette 4.

[0045] The upper surface of the rotating head cover plate 103 is provided with a through hole corresponding to the support screw 51, the support screw 51 extends outwardly through the through hole on the rotating head cover plate 103, and the rotating head cover plate 103 is fixed on the support screw 51 through the nut 52.

[0046] Working principle:

[0047] In use, the cuvette 4 is first placed in the corresponding mounting groove 104, and then the plant stem segment 3 is placed in the containing cavity 2 through the channel a formed between the two supporting screws 51, and the two ends of the plant stem segment 3 are located in the corresponding cuvettes 4, then the pressing plate 53 is sleeved on the supporting screw 51, and the pressing plate 53 is fixed by the nut 52, that is, the plant stem segment 3 can be fixed, and then the plant stem segment 3 and the pressing plate 53 are repeatedly placed in the containing cavity 2, that is, the device can accommodate multiple plant stem segment 3 samples.

[0048] The cuvette 4 contains a certain amount of water according to experimental requirements.

[0049] Example two:

[0050] Please refer to Figure 4 - Figure 7 As a further improvement of example one, the plant stem segment 3 is installed on the diameter of the rotating head body 1, that is, the device can be enlarged according to the actual proportion to accommodate larger and longer plant stem segments 3 for centrifugation; when the device is used to accommodate plant stem segments 3 with a diameter of 5-30mm and a length of 600mm, the device has a large volume, and it is inconvenient to disassemble when connected with the existing device.

[0051] Therefore, the bottom of the rotating head body 101 is provided with a tapered hole groove 106 for connecting with the centrifuge driving mechanism 7, and the top center of the rotating head body 101 is provided with a locking groove 107 in communication with the tapered hole groove 106, and the locking groove 107 is provided with a locking component 8 for locking the centrifuge driving mechanism 7.

[0052] The locking component 8 comprises a hexagonal sleeve 81 installed in the tapered hole groove 106 by screws, and the center of the hexagonal sleeve 81 is provided with a butt joint hole 86 for installing the centrifuge driving mechanism 7, and the butt joint hole 86 is provided with an internal hexagonal bolt 82, which is threadedly connected to the centrifuge driving mechanism 7 through the butt joint hole 86 to lock it.

[0053] The top center of the hexagonal sleeve 81 is provided with a mounting groove for installing the head of the internal hexagonal bolt 82, and the head of the internal hexagonal bolt 82 and the inner wall of the mounting groove are sequentially provided with an elastic check ring 83, a disc washer 84 and an aluminum gasket 85.

[0054] Further comprising a dismounting ejection block 9, the dismounting ejection block 9 comprises a driving part 92, a force receiving part 91 and an ejection part 93, the ejection part 93 is matched with the butt joint hole 86 of the hexagonal sleeve 81, and the outer side of the driving part 92 is provided with a thread matched with the inner wall of the mounting groove of the hexagonal sleeve 81, the dismounting ejection block 9 is connected with the mounting groove of the hexagonal sleeve 81 through the driving part 92 to make the ejection part 93 in the butt joint hole 86 extrude the centrifuge driving mechanism 7 in the butt joint hole 86.

[0055] In use, first need to make the taper hole groove 106 on the head body 101 and the butt joint hole 86 in the hex sleeve 81 inserted on the centrifuge drive mechanism 7 tight fit, then in turn install aluminum pad 85, disc washer 84, elastic check ring 83 and internal hexagonal bolt 82, through the internal hexagonal bolt 82 inserted on the centrifuge drive mechanism 7 further make the head body 1 locked on the centrifuge drive mechanism 7;

[0056] When disassembled, first remove the internal hexagonal bolt 82, then make the ejection part 93 in the disassembly ejection block 9 inserted in the butt joint hole 86 of the hex sleeve 81, and then make the driving part 92 in the installation groove of the hex sleeve 81 rotate through the force receiving part 91, so as to eject the centrifuge drive mechanism 7, thereby facilitating disassembly.

[0057] Among them, the force receiving part 91 is provided with an internal hexagonal screw hole groove;

[0058] Among them, the head body 1 can be installed with a lifting ring 6, which can further facilitate the separation of the head body 1 and the centrifuge drive mechanism 7 through tool equipment

[0059] Among them, the aluminum pad 85 and the disc washer 84 are used to add axial pre-tightening force, so that the fastening effect is better and the screw is prevented from loosening; the elastic check ring 83 is used to fix the aluminum pad 85 and the disc washer 84.

[0060] Example three:

[0061] A plant xylem centrifugal embolism induction method:

[0062] The head of the above-mentioned plant xylem embolism induction method is used to measure the water conductivity of the plant stem 3 by centrifugal force;

[0063] S1: In the plant xylem centrifugal embolism induction method, the plant stem 3 is placed inside the accommodating cavity 2 of the above-mentioned head, and is fixed by the pressing plate 53 cooperating with the support screw 51 and the nut 52, and the both ends of the plant stem 3 extend into the wall groove 44 of the cuvette 4; when the plant stem 3 rotates at a certain angular velocity, the centrifugal force makes the water in the bottom groove 44 of the cuvette 4 rise to the wall groove 44, and the both ends of the plant stem 3 are immersed.

[0064] S2: Set the instrument speed so that the tension T of the middle part of the plant stem 3 is 0.1 MPa, and take out the plant stem 3 from the instrument after centrifuging for 15 min:

[0065] S3: Measure the maximum water conductivity Kmax by using a low-pressure flowmeter; repeat the step and gradually increase the instrument speed, and measure the water conductivity Kh of the induced embolism under different tensions T by using a low-pressure flowmeter.

[0066] S4: According to the percentage loss of xylem hydraulic conductivity corresponding to different water potential PLC, a Weibull curve fitting is performed to obtain the vulnerability curve VC of the branch.

[0067] PLC / 100 = 1 - exp[1-(T / B) C ]

[0068] PLC / 100 = a{1-exp[1-(T / B1) C1 ]} + (1+a){1-exp[1-(T / B2) C2 ]}

[0069] In the formula, T is the tension, B and C are Weibull constants, and a is the proportion of the first component in the double Weibull distribution. The water potential P50 of the branch PLC is 50% is:

[0070] P50- = B[ln(2)] 1 / c .

[0071] The above is only an embodiment of the present application and is not intended to limit the present application. The present application can be variously changed and modified by those skilled in the art. Any modification, equivalent replacement, improvement, etc. made within the spirit and principle of the present application shall be included in the scope of the claims of the present application.

Claims

1. A rotor for measuring and analyzing plant xylem embolism vulnerability curve, comprising a rotor body (1), characterized in that: the rotor body (1) is provided with a containing cavity (2) for placing plant stem segments (3) to be measured and analyzed; the inside of the containing cavity (2) is provided with a plurality of cuvettes (4), the number of cuvettes (4) is even, and the cuvettes (4) comprise two vertically arranged bottom plates (41), the ends of the two bottom plates (41) are vertically fixedly connected inwardly with baffles (42), and the two sides of the two bottom plates (41) are fixedly connected with side plates (43), the cuvettes (4) form two vertical grooves (44) through the bottom plates (41), the side plates (43) and the baffles (42); both ends of the plant stem segment (3) are placed inside the corresponding cuvette (4); the inside of the containing cavity (2) is provided with a mounting assembly (5) for fixing a plurality of plant stem segments (3). The mounting assembly (5) comprises a plurality of support screws (51), the plurality of support screws (51) are threadedly mounted on the upper surface of the rotor body (101), and the support screws (51) are not located on the same diameter of the rotor body (1) as the cuvettes (4), and the support screws (51) form a passage a for the plant stem segments (3) to pass through; a plurality of pressing plates (53) can be sleeved on the support screws (51) corresponding to the number of plant stem segments (3), and the plant stem segments (3) and the pressing plates (53) are sequentially and repeatedly placed inside the containing cavity (2).

2. A rotor for measuring the embolism vulnerability curve of the xylem of a plant according to claim 1, characterized in that: The rotor body (1) comprises a rotor body (101), a ring-shaped side wall (102) and a rotor cover plate (103), the ring-shaped side wall (102) is fixedly installed on the edge of the upper surface of the rotor body (101), and the rotor cover plate (103) is detachably installed on the top of the ring-shaped side wall (102); the rotor body (101), the ring-shaped side wall (102) and the rotor cover plate (103) form the containing cavity (2) for placing the plant stem segments (3).

3. A rotor for measuring the embolism vulnerability curve of the xylem of a plant according to claim 2, characterized in that: A plurality of mounting grooves (104) for mounting the cuvettes (4) are formed on the upper surface of the rotor body (101).

4. A rotor for measuring the embolism vulnerability curve of the xylem of a plant according to claim 3, characterized in that: A fitting groove (105) is formed on the top of the ring-shaped side wall (102), the rotor cover plate (103) is installed inside the fitting groove (105), and the distance between the rotor cover plate (103) and the inner wall bottom of the taper hole groove (106) is equal to the height of the cuvette (4).

5. A rotor for measuring the embolism vulnerability curve of the xylem of a plant according to claim 3, characterized in that: A through hole is formed on the upper surface of the rotor cover plate (103) corresponding to the support screw (51), the support screw (51) extends outwardly through the through hole on the rotor cover plate (103), and the support screw (51) is fixed to the rotor cover plate (103) through a nut (52).

6. A rotor for measuring the embolism vulnerability curve of the xylem of a plant according to claim 4, characterized in that: The bottom of the rotor body (101) is provided with a taper hole groove (106) for connecting with a centrifuge driving mechanism (7), the top center of the rotor body (101) is provided with a locking groove (107) communicating with the taper hole groove (106), and the inside of the locking groove (107) is provided with a locking part (8) for locking the centrifuge driving mechanism (7).

7. A rotor for measuring the embolism vulnerability curve of the xylem of a plant according to claim 3, characterized in that: ​ 8. A rotor for measuring the embolism vulnerability curve of the xylem of a plant according to claim 7, characterized in that: The locking component (8) comprises a hexagonal sleeve (81) installed in the taper hole groove (106) by a screw, a butt joint hole (86) is formed in the center of the hexagonal sleeve (81) and is used for installing the centrifugal machine driving mechanism (7), an internal hexagonal bolt (82) is installed in the butt joint hole (86), and the internal hexagonal bolt (82) is screwed on the centrifugal machine driving mechanism (7) through the butt joint hole (86) and locks the centrifugal machine driving mechanism (7).

9. A rotor for measuring the embolism vulnerability curve of the xylem of a plant according to claim 8, characterized in that: A mounting groove for mounting the head of the internal hexagonal bolt (82) is formed in the top center of the hexagonal sleeve (81), and an elastic baffle ring (83), a dished washer (84) and an aluminum gasket (85) are sequentially mounted between the head of the internal hexagonal bolt (82) and the inner wall of the mounting groove.

10. A rotor for measuring the embolism vulnerability curve of the xylem of a plant according to claim 9, characterized in that: Further comprising a dismounting ejection block (9), the dismounting ejection block (9) comprises a driving part (92), a stress receiving part (91) and an ejection part (93), the ejection part (93) is matched with the butt joint hole (86) of the hexagonal sleeve (81), the outer side of the driving part (92) is provided with a matching thread with the inner wall of the mounting groove of the hexagonal sleeve (81), and the dismounting ejection block (9) is screwed with the mounting groove of the hexagonal sleeve (81) through the driving part (92), so that the ejection part (93) in the butt joint hole (86) extrudes and ejects the centrifugal machine driving mechanism (7) in the butt joint hole (86).

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