Biological immobilized enzyme catalytic biochemical reactor

Abstract

The utility model discloses a biological immobilized enzyme catalytic biochemical reactor, and relates to the technical field of medical intermediates. A biological immobilized enzyme catalysis biochemical reactor comprises a reaction tank covered with a tank cover, and further comprises a sleeve rotationally connected to the tank cover, and a biological immobilized enzyme catalysis biochemical reactor arranged on the sleeve, the square column is connected into the sleeve in a sliding mode, and a square groove matched with the square column for use is formed in the sleeve; according to the utility model, the contact range between the immobilized enzyme and the substrate is effectively enlarged, so that the immobilized enzyme and the substrate can be more fully contacted and reacted, the problem of non-uniform mixing is greatly improved, and when the immobilized enzyme needs to be taken out, the containing net box is firstly arranged, and then the threaded connection between the containing net box and the connecting rod is disassembled, so that the immobilized enzyme can be taken out. By means of the design, operation is easy and convenient, and the problems that due to the fact that the immobilized enzyme is difficult to take out, clean and replace, the enzyme activity is reduced, and the reaction cost is increased are solved.

Description

Technical Field

[0001] This invention belongs to the field of pharmaceutical intermediates technology, specifically, it relates to a bio-immobilized enzyme catalytic biochemical reactor. Background Technology

[0002] In the field of pharmaceutical intermediates, bio-immobilized enzyme-catalyzed biochemical reactions are widely used in many production and research scenarios. Currently, stirred tank reactors are one of the commonly used devices for such reactions.

[0003] However, in terms of mixing effect, most existing stirred tank reactors use relatively simple stirring blades. This structure makes it difficult to achieve sufficient and uniform mixing when stirring the immobilized enzyme and substrate. Due to the limited range and mode of action of the stirring blades, the immobilized enzyme and substrate in some areas of the reaction system are not in sufficient contact, which affects the overall efficiency and effect of the reaction, making it difficult to achieve the ideal quality and yield of the reaction products.

[0004] Regarding the maintenance of immobilized enzymes, the design of existing stirred tank reactors is not convenient for removing, cleaning, and replacing immobilized enzymes. When the activity of immobilized enzymes decreases or they become contaminated after multiple reactions and require treatment, operators often need to spend a lot of time and effort to disassemble and install related components, which increases the complexity and difficulty of the operation. Moreover, the complex operation process may cause additional damage to the immobilized enzymes, further reducing their activity, and also increasing the cost and time of the reaction. In view of this, this utility model is proposed. Utility Model Content

[0005] The technical problem to be solved by this invention is to overcome the shortcomings of the prior art and provide a bio-immobilized enzyme catalytic biochemical reactor that can overcome or at least partially solve the above problems.

[0006] To solve the above-mentioned technical problems, the basic concept of the technical solution adopted by this utility model is as follows: a bio-immobilized enzyme catalytic biochemical reactor, including a reaction vessel covered with a lid, and further including: a sleeve rotatably connected to the lid; a square column slidably connected inside the sleeve, the sleeve having a square groove for cooperating with the square column; multiple rows of connecting rods equidistantly fixedly connected to the square column at its position inside the reaction vessel; a mesh box containing the immobilized enzyme, the opening of the mesh box being detachably connected to the end of the connecting rods by threads; a drive mechanism for driving the sleeve to rotate, mounted on the lid; and a lifting assembly for controlling the up-and-down movement of the square column, mounted on the lid.

[0007] Furthermore, the driving mechanism includes a motor, a first gear, and a second gear. The motor is fixedly connected to the can lid via a support plate, the first gear is fixedly connected to the output end of the motor, and the second gear is fixedly connected to the sleeve and meshes with the first gear.

[0008] Furthermore, the lifting assembly includes a telescopic spring, a support rod, a roller, and a triangular inclined block. The telescopic spring is disposed inside the upper end of the sleeve, and its two ends are fixedly connected to the sleeve and the square column, respectively. The sleeve has multiple through slots equidistantly spaced around its circumference. The support rod is slidably connected in the through slots and fixedly connected to the square column. The roller is installed at the end of the support rod away from the square column and rolls on the can lid. The triangular inclined block is fixedly connected to the can lid equidistantly around its circumference and works in conjunction with the roller.

[0009] To facilitate the smooth up-and-down movement of the immobilized enzyme particles on the substrate of the reaction vessel, the triangular inclined block is further described as an isosceles triangular block.

[0010] To facilitate the vibration of the fixed enzyme particles in the container, thereby allowing the clumped fixed enzyme particles to disperse, the triangular inclined block is further designed as a right-angled triangular block.

[0011] To facilitate the shaking of the container under vibration by the connecting rod, thereby further improving the uniformity of the contact reaction between the immobilized enzyme and the substrate, the connecting rod is further described as an elastic rod.

[0012] To facilitate the automatic lifting of the tank lid, multiple electric telescopic rods are fixedly connected to the outer side of the reaction vessel at equal intervals around the circumference, and the telescopic ends of the electric telescopic rods are fixedly connected to the tank lid.

[0013] After adopting the above technical solution, the present invention has the following beneficial effects compared with the prior art: The present invention allows the immobilized enzyme to rotate and move up and down in the reaction vessel, effectively increasing the contact range between the immobilized enzyme and the substrate, enabling the immobilized enzyme and the substrate to contact and react more fully, greatly improving the problem of uneven mixing, and improving the reaction efficiency and reaction effect. When it is necessary to remove the immobilized enzyme, first open the lid of the vessel, then remove the mesh box from the reaction vessel, and then remove the immobilized enzyme located in the mesh box for cleaning or replacement by disassembling the threaded connection between the mesh box and the connecting rod. This design is simple and convenient to operate, reducing the problems of reduced enzyme activity and increased reaction cost that may be caused by the difficulty in removing, cleaning and replacing the immobilized enzyme.

[0014] The specific embodiments of this utility model will be described in further detail below with reference to the accompanying drawings. Attached Figure Description

[0015] In the attached diagram:

[0016] Figure 1 This is a schematic diagram of the structure of the present invention. Figure 1 ;

[0017] Figure 2 This is a schematic diagram of the structure of the present invention. Figure 2 ;

[0018] Figure 3 This is a schematic diagram of the internal structure of the reaction vessel of this utility model;

[0019] Figure 4 This is a cross-sectional structural diagram of a portion of the present invention.

[0020] In the diagram: 1. Reaction vessel; 2. Vessel lid; 3. Electric telescopic rod; 4. Motor; 5. First gear; 6. Sleeve; 601. Through groove; 7. Second gear; 8. Square column; 9. Telescopic spring; 10. Support rod; 11. Roller; 12. Container for holding mesh; 13. Connecting rod; 14. Isosceles triangle block; 15. Right triangle block. Detailed Implementation

[0021] To make the objectives, technical solutions, and advantages of the embodiments of this utility model clearer, the technical solutions in the embodiments will be clearly and completely described below with reference to the accompanying drawings. The following embodiments are used to illustrate this utility model, but are not intended to limit the scope of this utility model.

[0022] Example 1:

[0023] Reference Figures 1-4 A bio-immobilized enzyme catalytic bioreactor includes a reaction vessel 1, with a lid 2 covering the reaction vessel 1. It also includes: a sleeve 6 rotatably connected to the lid 2; a square column 8 slidably connected inside the sleeve 6, with a square groove inside the sleeve 6 for use with the square column 8; multiple rows of connecting rods 13 equidistantly fixedly connected to the square column 8 at its position inside the reaction vessel 1; a mesh box 12 containing the immobilized enzyme, the opening of the mesh box 12 being detachably connected to the end of the connecting rods 13 via threads; a drive mechanism for driving the sleeve 6 to rotate, mounted on the lid 2; and a lifting assembly for controlling the up-and-down movement of the square column 8, mounted on the lid 2.

[0024] The drive mechanism includes a motor 4, a first gear 5, and a second gear 7. The motor 4 is fixedly connected to the can lid 2 via a support plate. The first gear 5 is fixedly connected to the output end of the motor 4. The second gear 7 is fixedly connected to the sleeve 6 and meshes with the first gear 5.

[0025] The lifting assembly includes a telescopic spring 9, a support rod 10, a roller 11, and a triangular inclined block. The telescopic spring 9 is located inside the upper part of the sleeve 6, and its two ends are fixedly connected to the sleeve 6 and the square column 8, respectively. The sleeve 6 has multiple through slots 601 equidistantly arranged in a circle. The support rod 10 is slidably connected in the through slots 601 and fixedly connected to the square column 8. The roller 11 is installed on the end of the support rod 10 away from the square column 8 and rolls on the can lid 2. The triangular inclined block is fixedly connected to the can lid 2 equidistantly in a circle and works in conjunction with the roller 11.

[0026] In use, first, the required immobilized enzyme is loaded into the holding net box 12 and the holding net box 12 is threadedly connected to the connecting rod 13. Then, the substrate to be catalyzed is placed into the reaction vessel 1. Finally, the vessel lid 2 is closed, and the reactor can be started to catalyze the substrate.

[0027] The reactor's drive mechanism consists of a motor 4, a first gear 5, and a second gear 7. The motor 4 is securely fixed to the tank cover 2 via a support plate, serving as the power source for the entire drive system. When the motor 4 is powered on, its output shaft begins to rotate, thereby driving the first gear 5, which is fixedly connected to the output end, to rotate synchronously. Since the first gear 5 and the second gear 7 mesh with each other, according to the principle of gear transmission, the rotation of the first gear 5 will cause the second gear 7 to also begin to rotate. The second gear 7 is fixedly connected to the sleeve 6, so the sleeve 6 will rotate circumferentially on the tank cover 2 as the second gear 7 rotates. This process realizes the transmission of power from the motor 4 to the sleeve 6, providing the basic power for the subsequent rotation of the immobilized enzyme.

[0028] The lifting assembly of the reactor includes a telescopic spring 9, a support rod 10, a roller 11, and a triangular inclined block. The telescopic spring 9 is located inside the upper part of the sleeve 6, and its two ends are fixedly connected to the sleeve 6 and the square column 8 respectively, playing the role of elastic support and reset. The sleeve 6 has multiple through slots 601 equidistantly opened in a circle. The support rod 10 is slidably connected in these through slots 601, and one end of the support rod 10 is fixedly connected to the square column 8, while the other end is equipped with a roller 11. The triangular inclined block is fixedly connected to the tank cover 2 in a circle equidistantly and works in conjunction with the roller 11.

[0029] When the sleeve 6 rotates under the drive of the drive mechanism, the roller 11 installed at the end of the support rod 10 will roll on the can lid 2 as the sleeve 6 rotates. When the roller 11 rolls onto the triangular inclined block, the triangular inclined block has a certain inclination angle, which will generate an upward thrust on the roller 11. This thrust is transmitted to the square column 8 through the support rod 10, so that the square column 8 overcomes the elastic force of the telescopic spring 9 and slides upward in the square groove inside the sleeve 6. At this time, the telescopic spring 9 is compressed. When the roller 11 rolls away from the triangular inclined block, the telescopic spring 9 is no longer compressed by the external force and begins to recover its deformation. Its elastic potential energy is converted into kinetic energy, pushing the square column 8 to move downward and return to the initial position.

[0030] Multiple rows of connecting rods 13 are fixedly connected at equal intervals to the square column 8 located inside the reaction vessel 1. The opening of the container 12 containing the fixed enzyme is detachably connected to the end of the connecting rod 13 by threads. Since there is a sliding connection between the square column 8 and the sleeve 6, and the sleeve 6 rotates under the action of the drive mechanism, while the square column 8 moves up and down under the action of the lifting component, the connecting rod 13 and the fixed enzyme in the container 12 connected to it will both rotate in a circle and reciprocate up and down in the substrate inside the reaction vessel 1.

[0031] Through this complex movement mechanism, the movement trajectory of the immobilized enzyme within reaction vessel 1 is greatly enriched, enabling it to come into contact with substrates at different locations within reaction vessel 1 more extensively. This significantly increases the contact range and frequency between the immobilized enzyme and the substrate, thereby promoting a more complete and efficient biocatalytic reaction.

[0032] This reactor allows the immobilized enzyme to rotate and move up and down within the reaction vessel 1, effectively increasing the contact range between the immobilized enzyme and the substrate. This allows for more thorough contact and reaction between the immobilized enzyme and the substrate, greatly improving the problem of uneven mixing and enhancing reaction efficiency and effect. When it is necessary to remove the immobilized enzyme, first open the vessel lid 2, then remove the mesh box 12 from the reaction vessel 1. By disassembling the threaded connection between the mesh box 12 and the connecting rod 13, the immobilized enzyme located in the mesh box 12 can be removed for cleaning or replacement. This design is simple and convenient to operate, reducing the problems of reduced enzyme activity and increased reaction costs that may result from the difficulty in removing, cleaning, and replacing the immobilized enzyme.

[0033] Example 2:

[0034] Reference Figures 1-4 A bio-immobilized enzyme catalytic bioreactor, which is basically the same as in Example 1, but further: the triangular inclined block is an isosceles triangular block 14.

[0035] By designing the triangular inclined block as an isosceles triangle, during the rotation of the square column 8 driven by the sleeve 6, the immobilized enzyme particles can be smoothly moved up and down in the substrate of the reaction vessel 1 with the help of the telescopic spring 9, further increasing the contact range between the immobilized enzyme particles and the substrate and improving the uniformity of the substrate reaction.

[0036] Example 3:

[0037] Reference Figures 1-4 A bio-immobilized enzyme catalytic bioreactor, basically the same as in Example 1, but with the following further difference: the triangular inclined block is a right-angled triangular block 15, such as... Figure 2 As shown, by designing the triangular inclined block as a right angle, it can not only hold the mesh box 12 in the substrate in the reaction vessel 1 and shake it up and down, but also vibrate the immobilized enzyme particles in the mesh box 12, so that the clumped immobilized enzyme particles can be dispersed, so that the immobilized enzyme particles can fully contact the substrate to react.

[0038] The connecting rod 13 is an elastic rod. By making the connecting rod 13 a flexible material, when the roller 11 leaves the right-angled triangular block 15 and falls, the connecting rod 13 can drive the holding net box 12 to shake under the action of vibration, thereby facilitating further improvement of the uniformity of the contact reaction between the fixed enzyme and the substrate and improving the reaction efficiency.

[0039] Example 4:

[0040] Reference Figures 1-4 A bio-immobilized enzyme catalytic biochemical reactor, which is basically the same as in Example 1, but further: multiple electric telescopic rods 3 are fixedly connected to the outer side of the reaction vessel 1 in a circumferentially equidistant manner, and the telescopic ends of the electric telescopic rods 3 are fixedly connected to the vessel cover 2.

[0041] With the electric telescopic rod 3, when it is necessary to open the tank cover 2 for adding materials or to remove part of the contents of the reaction tank 1, the electric telescopic rod 3 can be activated automatically, without the need for staff to manually lift and lower the tank cover 2, which effectively improves the ease of operation of the reactor.

[0042] The above description is only a preferred embodiment of the present utility model and is not intended to limit the present utility model in any way. Although the present utility model has been disclosed above with reference to a preferred embodiment, it is not intended to limit the present utility model.

Claims

1. A biocatalyst immobilized enzyme catalyzed biochemical reactor, characterized in that, The utility model relates to a kind of enzyme reaction device, including reaction tank (1), the tank cover (2) is covered on the reaction tank (1), further including: Sleeve (6) is rotatably connected on the tank cover (2); Square column (8) is slidably connected in the sleeve (6), square slot is set in the sleeve (6) and cooperates with square column (8); Multiple connecting rods (13) are equidistantly fixedly connected on the position of square column (8) in the reaction tank (1); Net box (12) is loaded with fixed enzyme, and the box mouth of the net box (12) is detachably connected with the end of connecting rod (13) by thread; Driving mechanism for driving the sleeve (6) to rotate is installed on the tank cover (2); Lifting assembly for controlling the square column (8) to move up and down is installed on the tank cover (2).

2. The bioimmobilized enzyme catalyzed biochemical reactor according to claim 1, characterized in that, The driving mechanism includes motor (4), first gear (5) and second gear (7), the motor (4) is fixedly connected on the tank cover (2) by support plate, the first gear (5) is fixedly connected on the output end of motor (4), the second gear (7) is fixedly connected on the sleeve (6), and is engaged with the first gear (5).

3. The bioimmobilized enzyme catalyzed biochemical reactor according to claim 1, wherein, The lifting assembly includes telescopic spring (9), support rod (10), roller (11) and triangular inclined block, the telescopic spring (9) is arranged on the inner upper end of sleeve (6), both ends of the telescopic spring (9) are fixedly connected with sleeve (6) and square column (8) respectively, a plurality of through grooves (601) are circumferentially equidistantly formed on the sleeve (6), the support rod (10) is slidably connected in the through groove (601), and is fixedly connected with the square column (8), the roller (11) is installed on the end of support rod (10) away from square column (8), and rolls on the tank cover (2), the triangular inclined block is circumferentially equidistantly fixedly connected on the tank cover (2), and cooperates with the roller (11).

4. The bioimmobilized enzyme catalyzed biochemical reactor according to claim 3, wherein, The triangular inclined block is isosceles triangular block (14).

5. The bioimmobilized enzyme catalyzed biochemical reactor according to claim 3, wherein, The triangular inclined block is right-angled triangular block (15).

6. The bioimmobilized enzyme catalyzed biochemical reactor according to claim 5, wherein, The connecting rod (13) is elastic rod.

7. The bioimmobilized enzyme catalyzed biochemical reactor according to claim 1, wherein, Multiple electric telescopic rods (3) are circumferentially equidistantly fixedly connected on the outside of the reaction tank (1), and the telescopic end of the electric telescopic rod (3) is fixedly connected with the tank cover (2). Multiple electric telescopic rods (3) are circumferentially equidistantly fixedly connected on the outside of the reaction tank (1), and the telescopic end of the electric telescopic rod (3) is fixedly connected with the tank cover (2).

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