Suspension cell culture device convenient for changing liquid
By designing a first and second motor drive screw system in the suspension cell culture device, the culture box moves along a cross path, solving the problem of the lack of a shaking function in the suspension cell culture device, achieving full fusion of culture medium and cells, and improving culture quality.
Patent Information
- Application Number
- CN202520027537.0
- Authority / Receiving Office
- CN · China
- Patent Type
- Utility models(China)
- Current Assignee / Owner
- Filing Date
- 2025-01-07
- Publication Date
- 2026-02-17
- Estimated Expiration
- 2035-01-07
AI Technical Summary
Common suspension cell culture devices that facilitate medium changes lack a shaking function, which prevents the new culture medium from fully mixing with the cells, easily leading to cell accumulation and affecting culture quality.
The first motor drives the first lead screw, and the second motor drives the second lead screw in synergy, causing the culture box to move along a cross path, which in turn shakes the culture medium to achieve full fusion with the cells.
This method achieves full fusion of the culture medium and cells, prevents cell accumulation, and improves the quality of suspension cell culture.
Smart Images
Figure CN223921426U_ABST
Abstract
Description
TECHNICAL FIELD
[0001] The utility model relates to the technical field of suspension cell culture, especially to a suspension cell culture device convenient for liquid change. BACKGROUND
[0002] Cell culture technology refers to the technology for maintaining cell growth under in vitro conditions, and according to whether the growth characteristics of cells depend on a support, cell culture can be divided into adherent cell culture and suspension cell culture. The diameter of most suspension cells is in the range of 10-20 um, and the survival of cells in cell suspension depends on the nutrients and environmental conditions provided by the culture medium. During the culture process, the culture medium needs to be replaced, that is, the cell suspension is discharged and new culture medium is added.
[0003] The common suspension cell culture device convenient for liquid change only includes a liquid change function and can change the liquid of the suspension cells, but lacks a shaking function, which cannot guarantee that the new culture medium can fully mix with the cells, and part of the cells may accumulate and cannot fully mix with the newly added culture medium, affecting the culture quality.
[0004] Therefore, in view of the above-mentioned problem that the common suspension cell culture device convenient for liquid change lacks a shaking function and cannot guarantee that the new culture medium can fully mix with the cells, a suspension cell culture device convenient for liquid change is designed. The first motor drives the first lead screw to rotate forward or reversely, the first lead screw drives the culture box to move left or right through the driving belt, at the same time, the second motor drives the second lead screw to rotate forward or reversely, the second lead screw drives the culture box to move forward or backward through the driving block, so that the culture box moves along a cross-shaped route, and the culture medium inside the culture box is shaken during the movement of the culture box, so that it fully mixes with the cells. SUMMARY
[0005] In order to overcome the problem that the common suspension cell culture device convenient for liquid change lacks a shaking function and cannot guarantee that the new culture medium can fully mix with the cells, a suspension cell culture device convenient for liquid change is designed.
[0006] The utility model discloses a technical scheme for a suspended cell culture device facilitating liquid replacement, comprising a support base plate, a left side strip, a right side strip, a driving strip, a first motor, a first screw rod, a rear side block, a front side block, a driving block, a second motor, a second screw rod and a sliding rod.
[0007] Preferably, the first motor drives the first screw rod to rotate forward or reversely, the first screw rod drives the culture box to move left or right through the driving strip, at the same time, the second motor drives the second screw rod to rotate forward or reversely, the second screw rod drives the culture box to move forward or backward through the driving block, so that the culture box moves along a cross route, and the culture medium in the culture box is shaken to fully mix with the cells, thereby solving the problem that the conventional suspended cell culture device facilitating liquid replacement only has the liquid replacement function and can replace the liquid of the suspended cells, but lacks the shaking function, cannot guarantee that the new culture medium fully mixes with the cells, and the part of the cells that is accumulated cannot fully mix with the newly added culture medium, thereby affecting the culture quality.
[0008] Preferably, the left end of the first screw rod is rotationally connected with the left side strip, and the rear end of the second screw rod is rotationally connected with the rear side block.
[0009] Preferably, the right end of the sliding rod is welded with the right side strip.
[0010] Preferably, the top of the driving block is provided with the culture box, four support legs are arranged at the bottom of the culture box, and universal wheels are arranged at the bottom end of the support legs.
[0011] Preferably, the bottom end of the universal wheel is attached to the top of the support base plate, and a liquid adding port is arranged at the right side of the middle of the top of the culture box.
[0012] Preferably, a liquid discharging port is arranged at the left side of the middle of the bottom of the culture box, and a liquid discharging hose is arranged at the bottom of the liquid discharging port.
[0013] Preferably, a filter plate is arranged in the middle of the culture box, and a flow guide triangular plate is arranged at the right side of the bottom end of the culture box.
[0014] The utility model discloses a technical scheme for a suspended cell culture device facilitating liquid replacement, comprising a support base plate, a left side strip, a right side strip, a driving strip, a first motor, a first screw rod, a rear side block, a front side block, a driving block, a second motor, a second screw rod and a sliding rod.
[0015] 1, by the first motor drive first screw forward or reverse rotation, the first screw drive strip drive culture box to move left or right, at the same time by the second motor drive second screw forward or reverse rotation, the second screw drive block drive culture box to move forward or backward, so that the culture box according to the cross route moves, in the process of culture box moves drive its inside culture medium liquid shake to make it and cell fully fused, to solve the common liquid exchange function of suspension cell culture device, only contains, can change liquid for suspension cell, but lack of shake evenly function, can not guarantee that the new culture medium liquid can be fully fused with cell, easy to appear part of the cell accumulation after can not fully fused with the new culture medium liquid, affect the culture quality problem. BRIEF DESCRIPTION OF DRAWINGS
[0016] Figure 1 The whole structure of the suspension cell culture device is shown.
[0017] Figure 2 The horizontal drive assembly structure of the suspension cell culture device is shown.
[0018] Figure 3 The vertical drive assembly structure of the suspension cell culture device is shown.
[0019] Figure 4 The culture box structure of the suspension cell culture device is shown.
[0020] Figure 5 The culture box structure of the suspension cell culture device is shown.
[0021] The drawing mark explanation: 1, support bottom plate; 2, left side strip; 3, right side strip; 4, drive strip; 5, first motor; 6, first screw; 7, rear block; 8, front block; 9, drive block; 10, second motor; 11, second screw; 12, slide; 13, culture box; 14, support leg; 15, universal wheel; 16, liquid inlet; 17, liquid outlet; 18, liquid discharge hose; 19, filter plate; 20, flow guide triangular plate. DETAILED DESCRIPTION
[0022] The utility model will be further explained in combination with the drawings and examples.
[0023] Please refer to Figures 1-5The utility model provides an embodiment: a kind of liquid-replacing convenient suspension cell culture device, including support base plate 1;Still including left side strip 2, right side strip 3, drive strip 4, first motor 5, first screw rod 6, rear side block 7, front side block 8, drive block 9, second motor 10, second screw rod 11 and slide 12, left side strip 2 is provided in the left side of support base plate 1 top, right side strip 3 is provided in the right side of support base plate 1 top, drive strip 4 is provided in the middle of support base plate 1 top, first motor 5 is provided in the front end of the right side of right side strip 3, and the output end of first motor 5 left side is connected with first screw rod 6 and penetrates right side strip 3, first screw rod 6 is connected with drive strip 4 by screw thread, rear side block 7 is provided in the rear side of drive strip 4 top, front side block 8 is provided in the front side of drive strip 4 top, drive block 9 is provided in the middle of drive strip 4 top, second motor 10 is provided in the front side center of front side block 8, and the output end of second motor 10 rear side is connected with second screw rod 11 and penetrates front side block 8, second screw rod 11 is connected with drive block 9 by screw thread, slide 12 is welded in the rear end of the right side of left side strip 2, and slide 12 is connected with drive strip 4 slidingly, first motor 5 drives first screw rod 6 to rotate forward or reversely, and first screw rod 6 drives culture box 13 to move left or right by drive strip 4, while second motor 10 drives second screw rod 11 to rotate forward or reversely, and second screw rod 11 drives culture box 13 to move forward or backward by drive block 9, so that culture box 13 moves according to the route of cross, culture medium liquid in culture box 13 is shaken in the process of moving to make it fully fused with cell, to solve the common liquid-replacing convenient suspension cell culture device, only include liquid-replacing function, can carry out liquid-replacing to suspension cell, but lack the function of shaking, cannot guarantee that new culture medium liquid can be fully fused with cell, prone to part of cell accumulation after new culture medium liquid is fully fused, affect the problem of culture quality.
[0024] Please refer to Figures 2-5In the embodiment, the bottom end of the universal wheel 15 is attached to the top of the support bottom plate 1, the right side of the middle of the top of the culture box 13 is provided with a liquid adding port 16, the left side of the middle of the bottom of the culture box 13 is provided with a liquid discharging port 17, the bottom of the liquid discharging port 17 is provided with a liquid discharging hose 18, the middle of the inside of the culture box 13 is provided with a filter plate 19, the right side of the bottom end of the inside of the culture box 13 is provided with a flow guide triangular plate 20, the cell suspension is added into the inside of the culture box 13 through the liquid adding port 16, after the cell suspension is filtered by the filter plate 19, the suspended cells are retained on the top of the filter plate 19, and the cell suspension falls to the bottom end of the inside of the culture box 13, when it is necessary to change the liquid, the cell suspension is guided to the liquid discharging port 17 through the flow guide triangular plate 20 with a certain inclination, then the plug of the port of the liquid discharging hose 18 is opened, the cell suspension is discharged through the liquid discharging hose 18, after the cell suspension is discharged, the plug is plugged back into the port of the liquid discharging hose 18, then the new culture medium liquid is added into the inside of the culture box 13 through the liquid adding port 16, so that the liquid changing operation is completed.
[0025] Please refer to Figures 1-5 In the embodiment, the left end of the first lead screw 6 is rotationally connected with the left side strip 2, the rear end of the second lead screw 11 is rotationally connected with the rear side block 7, the right end of the slide rod 12 is welded with the right side strip 3, the top of the driving block 9 is provided with the culture box 13, the bottom of the culture box 13 is provided with four support legs 14, the bottom end of the support leg 14 is provided with the universal wheel 15, after the liquid changing is completed, the first lead screw 6 is driven by the first motor 5 to rotate forward or reversely, the culture box 13 is driven by the driving strip 4 to move left or right, at the same time, the second lead screw 11 is driven by the second motor 10 to rotate forward or reversely, the culture box 13 is driven by the driving block 9 to move forward or backward, so that the culture box 13 moves along the cross route, the culture medium liquid in the culture box 13 is shaken in the moving process, so that the culture medium liquid is fully mixed with the cells, the shaking function is realized, the problem that the culture quality is affected due to that the cells are not fully mixed with the new added culture medium liquid after the cells are accumulated is prevented.
[0026] In the process of work, the cell suspension is added into the inside of the culture box 13 through the liquid inlet 16, and after the filtration of the filter plate 19, the suspended cells are retained on the top of the filter plate 19, and the cell suspension falls to the bottom end inside the culture box 13, and the cells in the cell suspension survive depending on the nutrients and environmental conditions provided by the culture medium liquid. When it is necessary to change the liquid, the cell suspension is guided to the liquid outlet 17 through the guide triangle plate 20 with a certain inclination, then the plug at the port of the liquid outlet hose 18 is opened, and the cell suspension is discharged through the liquid outlet hose 18. After the cell suspension is discharged, the plug is plugged back into the port of the liquid outlet hose 18, and then the new culture medium liquid is added into the inside of the culture box 13 through the liquid inlet 16. After the liquid change is completed, the first motor 5 drives the first lead screw 6 to rotate forward or reversely, and the first lead screw 6 drives the culture box 13 to move left or right through the driving bar 4. At the same time, the second motor 10 drives the second lead screw 11 to rotate forward or reversely, and the second lead screw 11 drives the culture box 13 to move forward or backward through the driving block 9, so that the culture box 13 moves in a cross-shaped route. In the process of moving the culture box 13, the culture medium liquid inside the culture box 13 is shaken to fully mix with the cells, and when the cultured cells are taken out, the extraction pipe of the external extraction equipment is inserted into the inside of the liquid inlet 16, and then the cultured cells retained on the top of the filter plate 19 are extracted through the extraction pipe of the external extraction equipment, realizing the shaking function.
[0027] Through the above steps, the first motor 5 drives the first lead screw 6 to rotate forward or reversely, and the first lead screw 6 drives the culture box 13 to move left or right through the driving bar 4. At the same time, the second motor 10 drives the second lead screw 11 to rotate forward or reversely, and the second lead screw 11 drives the culture box 13 to move forward or backward through the driving block 9, so that the culture box 13 moves in a cross-shaped route. In the process of moving the culture box 13, the culture medium liquid inside the culture box 13 is shaken to fully mix with the cells, so as to solve the problem that the common suspended cell culture device with liquid changing function only contains the liquid changing function and can change the liquid of the suspended cells, but lacks the shaking function, cannot guarantee that the new culture medium liquid can fully mix with the cells, and is prone to the problem that part of the cells are accumulated and cannot fully mix with the newly added culture medium liquid, affecting the culture quality.
Claims
1. A device for culturing suspended cells, which facilitates liquid exchange, comprising a support base plate (1), characterized in that: It also includes the left bar (2), right bar (3), drive bar (4), the first motor (5), the first lead screw (6), rear block (7), front block (8), drive block (9), the second motor (10), the second lead screw (11) and slide rod (12), the left side of the top of the supporting bottom plate (1) is provided with left bar (2), the right side of the top of the supporting bottom plate (1) is provided with right bar (3), the middle of the top of the supporting bottom plate (1) is provided with drive bar (4), the front end of the right side of the right bar (3) is provided with the first motor (5), the output end of the left side of the first motor (5) is connected with the first lead screw (6) penetrating the right bar (3), the first lead screw (6) is in threaded connection with the drive bar (4), the rear side of the top of the drive bar (4) is provided with rear block (7), the front side of the top of the drive bar (4) is provided with front block (8), the middle of the top of the drive bar (4) is provided with drive block (9), the front side center of the front block (8) is provided with the second motor (10), the output end of the rear side of the second motor (10) is connected with the second lead screw (11) penetrating the front block (8), the second lead screw (11) is in threaded connection with the drive block (9), the rear end of the right side of the left bar (2) is welded with the slide rod (12), and the slide rod (12) is in sliding connection with the drive bar (4).
2. The suspended cell culture device of claim 1, wherein: The left end of the first lead screw (6) is rotatably connected with the left bar (2), and the rear end of the second lead screw (11) is rotatably connected with the rear block (7).
3. The suspended cell culture device of claim 1, wherein: The right end of the slide rod (12) is welded with the right bar (3).
4. The suspended cell culture device of claim 1, wherein: The top of the drive block (9) is provided with a culture box (13), and four supporting legs (14) are arranged at the bottom of the culture box (13).
5. The suspended cell culture device of claim 4, wherein: The bottom end of the universal wheel (15) is attached to the top of the supporting bottom plate (1), and the right side of the middle of the top of the culture box (13) is provided with a liquid inlet (16).
6. The suspended cell culture device of claim 5, wherein: The left side of the middle of the bottom of the culture box (13) is provided with a drainage outlet (17), and the bottom of the drainage outlet (17) is provided with a drainage hose (18).
7. The suspended cell culture device of claim 6, wherein: The middle of the inside of the culture box (13) is provided with a filter plate (19), and the right side of the inside bottom end of the culture box (13) is provided with a flow guide triangular plate (20).