Novel glass slide suitable for microscopic observation of plant root hair morphology
By designing root segment receiving grooves and drainage channels on the glass slide, the problem of disordered root segment distribution was solved, enabling efficient fixation and accurate measurement of root hair morphology, and improving the ease of operation and reliability of data in the experiment.
Patent Information
- Application Number
- CN202520651067.5
- Authority / Receiving Office
- CN · China
- Patent Type
- Utility models(China)
- Current Assignee / Owner
- Filing Date
- 2025-04-09
- Publication Date
- 2026-02-17
- Estimated Expiration
- 2035-04-09
AI Technical Summary
In existing technologies, conventional slides suffer from problems such as disordered root segment distribution, high observation difficulty, and large result errors in root hair morphology observation. Furthermore, traditional methods cannot effectively fix and arrange root segments in parallel, resulting in low observation efficiency and poor data accuracy.
A novel glass slide was designed, comprising a regular glass slide and an auxiliary slide. The auxiliary slide is equipped with a root segment receiving groove, a drainage channel, and a water outlet. The design of the root segment receiving groove avoids direct compression of the root segments, and the drainage channel and water outlet remove excess liquid, thereby achieving the fixation and parallel arrangement of multiple root segments and preventing the generation of air bubbles.
This method enables batch fixation and parallel arrangement of multiple root segments, avoiding root hair twisting and cortical shedding, improving the operability of observation and the accuracy of data, and reducing experimental errors.
Smart Images
Figure CN223926707U_ABST
Abstract
Description
TECHNICAL FIELD
[0001] The utility model relates to the field of glass slide technology, especially to a novel glass slide suitable for plant root hair morphology microscopic observation. BACKGROUND
[0002] Root hair is a tubular protruding structure on the surface of plant root system, and its main function is to expand the exploration area of root system to soil resources. Especially under low phosphorus and nitrogen conditions, the length and density of root hair are considered to directly affect the nutrient absorption capacity of plants. Based on the data statistics on the absorption fine root diameter and root hair length collected by the global fine root ecological database, the sample amount proportion of the two is 97.8% and 98.3% respectively when the value is less than 1 mm, which accounts for the majority of the total sample amount. At present, researchers mainly rely on microscope observation of root hair morphology, and scanning electron microscope can obtain fine root hair parameters, but its price is expensive, efficiency is very slow, and operation process is complex, so most scientific research and education units cannot afford such experiments. In addition, for plant ecology experiments, a large number of repeated samples need to be constructed, and the requirement for experimental efficiency is high. Therefore, plant ecology researchers usually use ordinary glass slides or special culture dishes for sample preparation, after staining, slicing, microscopic examination (ordinary optical microscope or stereoscopic microscope can be used), photographing (microscope combined with ocular digital camera) and picture analysis, the key traits such as root hair diameter, length and density can be determined.
[0003] In the prior art, the root hair morphology observation method based on glass slide or culture dish is mainly realized through the following process: first, the plant root section, usually absorption fine root, about 1 cm in length, is stained, such as pre-fixing with FAA fixing solution or directly staining fresh sample, then distilled water is added on the ordinary glass slide, the stained root section is manually arranged in the liquid drop and covered with a cover glass, or it is placed in a transparent culture dish and injected with sufficient distilled water to immerse the sample; after completing the sample placement, microscopic examination is carried out through optical microscope or stereoscopic microscope, and image is photographed by ocular digital camera, finally, image processing software is used to quantitatively analyze the root hair morphology parameters including length, diameter and density.
[0004] The existing experimental method mainly focuses on surface observation and quantitative analysis of morphological characteristics, and no special device has been found to efficiently and stably measure and record the morphological changes. The existing observation method has many shortcomings: 1. When the cover glass is not used, the root section is easy to deviate in the distilled water, and the field of view needs to be constantly adjusted in the microscope, which increases the workload; 2. Since the root hair is a microstructure, the imaging clarity is required to be high, and the cover glass is not added to easily cause uneven focal length of the sample, which affects the observation effect; 3. The cover glass can solve the above two problems, but during the process of adding the cover glass, the root section sample lacks fixation, the root section moves in the liquid, it is difficult to arrange in parallel, and different root sections are often pasted together, which increases the difficulty and workload of the subsequent experiment; 4. The root section is deformed or even the cortex is peeled off after being pressed by the cover glass, which is also not conducive to the observation of the root hair; 5. The root hair is twisted or blocked after being pressed by the cover glass, which increases the experimental error of subsequent morphological measurement and counting. The improved root hair morphological observation slide device is proposed in the background, aiming at overcoming the defects in the prior art and improving the convenience and stability of experimental operation. Utility model content
[0005] The utility model provides a novel slide suitable for plant root hair morphological microscopic observation, to solve the technical problem of the conventional slide in the prior art when using the root section distribution confusion, the observation root hair is difficult and the observation result error is bigger, reaches the batch fixation of multiple root sections, avoids generating the air bubble through the drainage flow guide, improves the operability.
[0006] The utility model provides a novel slide suitable for plant root hair morphological microscopic observation, to solve the technical problem of the conventional slide in the prior art when using the root section distribution confusion, the observation root hair is difficult and the observation result error is bigger, reaches the batch fixation of multiple root sections, avoids generating the air bubble through the drainage flow guide, improves the operability.
[0007] Common slide;
[0008] Auxiliary sheet, fixedly arranged on one side of the common slide, a root section containing groove, a drainage flow guide channel and a water outlet are formed in the auxiliary sheet, a plurality of root section containing grooves are arrayed in the auxiliary sheet, the root section containing grooves are communicated through the drainage flow guide channel, and the drainage flow guide channel is communicated with the water outlet at one end close to the edge of the auxiliary sheet.
[0009] According to the novel slide suitable for plant root hair morphological microscopic observation provided by the utility model: the width of the auxiliary sheet is same with the size of the common slide, and the length of the auxiliary sheet is less than the length of the common slide.
[0010] According to the novel slide suitable for plant root hair morphological microscopic observation provided by the utility model: the root section containing groove includes a rectangular groove, a first limiter and a second limiter, and the first limiter and the second limiter are fixedly arranged on two opposite side walls of the rectangular groove.
[0011] The utility model provides a novel slide glass suitable for plant root hair morphology microscopical observation, the length of first stopper and second stopper is different, the first stopper and second stopper are fixedly arranged with the interval in root section containing groove one side wall, the second stopper and first stopper are fixedly arranged with the interval in root section containing groove opposite another side wall, and the sum of the length of first stopper and second stopper is less than the interval of the both side walls of root section containing groove.
[0012] The utility model provides a novel slide glass suitable for plant root hair morphology microscopical observation, the material quality of auxiliary sheet is transparent waterproof material quality.
[0013] The utility model discloses the beneficial effect that:
[0014] The utility model provides a novel slide glass suitable for plant root hair morphology microscopical observation, including ordinary slide glass and auxiliary sheet, and the root section containing groove, drainage flow channel and water outlet are seted up on the auxiliary sheet, and the direct compression of traditional cover glass to root section is avoided through the design of root section containing groove, and the technical problem of the greater difficulty of observing root hair and the greater error of observation result of root section distribution confusion when using conventional slide glass in the prior art is solved, and the purpose that the operability is improved is achieved.
[0015] The additional aspects and advantages of the utility model will be partly given in the following description, and some will become obvious from the following description or be understood through the practice of the utility model. BRIEF DESCRIPTION OF DRAWINGS
[0016] In order to more clearly illustrate the technical scheme in the utility model or the prior art, the following will briefly introduce the drawings needed to be used in the embodiment or the prior art description, and obviously, the drawings in the following description are some embodiments of the utility model, and for the ordinary skilled in the art, other drawings can also be obtained according to these drawings without paying creative labor.
[0017] Fig. 1 It is a kind of novel slide glass for plant root hair morphology microscopical observation three-dimensional structure schematic view;
[0018] Fig. 2 It is a kind of novel slide glass for plant root hair morphology microscopical observation plan view.
[0019] Reference signs:
[0020] 1. Ordinary glass slide; 2. Auxiliary slide; 201. Root segment receiving groove; 2011. Rectangular groove; 2012. First limiter; 2013. Second limiter; 202. Drainage guide channel; 203. Water outlet. Detailed Implementation
[0021] To make the objectives, technical solutions, and advantages of this utility model clearer, the technical solutions of this utility model will be clearly and completely described below with reference to the accompanying drawings. Obviously, the described embodiments are only some embodiments of this utility model, not all embodiments. Based on the embodiments of this utility model, all other embodiments obtained by those skilled in the art without creative effort are within the protection scope of this utility model.
[0022] In the description of the embodiments of this utility model, it should be noted that the terms "upper," "lower," "left," "right," "vertical," "horizontal," "top," "bottom," "inner," and "outer," etc., indicate the orientation or positional relationship based on the orientation or positional relationship shown in the accompanying drawings. They are only for the convenience of describing the embodiments of this utility model and simplifying the description, and do not indicate or imply that the mechanism or element referred to must have a specific orientation, or be constructed and operated in a specific orientation. Therefore, they should not be construed as limitations on the embodiments of this utility model. In addition, the terms "first," "second," etc., are used for descriptive purposes only and should not be construed as indicating or implying relative importance.
[0023] In the description of the embodiments of this utility model, it should be noted that, unless otherwise explicitly specified and limited, the terms "connected" and "linked" should be interpreted broadly. For example, they can refer to a fixed connection, a detachable connection, or an integral connection; they can refer to a mechanical connection or an electrical connection; they can refer to a direct connection or an indirect connection through an intermediate medium. Those skilled in the art can understand the specific meaning of the above terms in the embodiments of this utility model based on the specific circumstances.
[0024] In this embodiment of the utility model, unless otherwise explicitly specified and limited, "above" or "below" the second feature can mean that the first feature is in direct contact with the second feature, or that the first feature is in indirect contact with the second feature through an intermediate medium. Furthermore, "above," "on top of," and "over" the second feature can mean that the first feature is directly above or diagonally above the second feature, or simply that the first feature is at a higher horizontal level than the second feature. "Below," "below," and "under" the second feature can mean that the first feature is directly below or diagonally below the second feature, or simply that the first feature is at a lower horizontal level than the second feature.
[0025] In the description of this specification, the references to terms such as "one embodiment," "some embodiments," "example," "specific example," or "some examples," etc., refer to specific features, structures, materials, or characteristics described in connection with that embodiment or example, which are included in at least one embodiment or example of the present invention. In this specification, the illustrative expressions of the above terms do not refer to the same embodiment or example. Furthermore, the specific features, structures, materials, or characteristics described may be combined in any suitable manner in one or more embodiments or examples. Moreover, without contradiction, those skilled in the art can combine and integrate the different embodiments or examples described in this specification, as well as the features of different embodiments or examples.
[0026] The following is combined Figs. 1-2 The embodiments shown illustrate the technical solution of this utility model:
[0027] This invention provides a novel glass slide suitable for microscopic observation of plant root hair morphology, comprising: a regular glass slide 1; and an auxiliary slide 2, which is fixedly disposed on one side of the regular glass slide 1. The auxiliary slide 2 has a root segment receiving groove 201, a drainage channel 202, and a water outlet 203. Multiple root segment receiving grooves 201 are arrayed on the auxiliary slide 2, and the root segment receiving grooves 201 are connected to each other through the drainage channel 202. The end of the drainage channel 202 near the edge of the auxiliary slide 2 is connected to the water outlet 203.
[0028] Understandably, this invention provides a novel glass slide suitable for microscopic observation of plant root hair morphology. By fixing an auxiliary slide 2 to the upper surface of a regular glass slide 1, multiple root segments are batch-fixed and arranged in parallel, preventing the root segments from moving or sticking together in the liquid. The design of the root segment receiving groove 201 avoids the direct pressure of traditional coverslips on the root segments, preventing root hair twisting, cortex shedding, and imaging obstruction. Simultaneously, a drainage channel 202 and a water outlet 203 are provided. Adding excess distilled water to the root segment receiving groove 201 and its drainage effect when the coverslip is applied prevents air bubbles from forming within the groove, improving operability. Through these technical improvements, a dedicated glass slide device for plant root hair morphology analysis is provided, offering convenient operation, reliable fixation, and non-destructive sample handling, significantly improving the accuracy and operability of experimental data.
[0029] The ordinary glass slide 1 is a common rectangular glass slide of 76 mm × 25 mm × 1.2 mm. The auxiliary slide 2 is fixedly set on the top surface of the ordinary glass slide 1. The size of the auxiliary slide 2 is smaller than that of the ordinary glass slide 1. The ordinary glass slide 1 and the auxiliary slide 2 can be integrally formed or separately formed and then fixedly connected by optical adhesive.
[0030] The auxiliary plate has a root segment receiving groove 201, a drainage channel 202, and a water outlet 203. The root segment receiving groove 201 is used to fix and arrange multiple root segments in batches, preventing the root segments from moving or sticking together in the liquid. The drainage channel 202 is connected to the root segment receiving groove 201 and is fixedly set on the lower side wall of the root segment receiving groove 201. The water outlet 203 is connected to the drainage channel 202 and is connected to the side wall of the auxiliary plate, which facilitates the addition of excess distilled water to the root segment receiving groove 201 and its drainage and removal function when the cover glass is applied, thus preventing the generation of air bubbles in the root segment receiving groove 201.
[0031] According to this utility model, a novel glass slide suitable for microscopic observation of plant root hair morphology is provided: the width of the auxiliary slide 2 is the same as that of the ordinary glass slide 1, and the length of the auxiliary slide 2 is less than that of the ordinary glass slide 1.
[0032] In this invention, the auxiliary sheet 2 has a size of 66 mm × 25 mm × 1 mm. The upper, lower, and right edges of the auxiliary sheet 2 and the ordinary glass slide 1 are aligned to ensure that the upper, lower, and right edges of the two are flat and fit together. If it is integrally formed, the overall strength of the glass slide can be improved.
[0033] According to the present invention, a novel glass slide suitable for microscopic observation of plant root hair morphology is provided: the root segment receiving groove 201 includes a rectangular groove 2011, a first limiter 2012 and a second limiter 2013, and the first limiter 2012 and the second limiter 2013 are fixedly provided on the two opposite side walls of the rectangular groove 2011.
[0034] According to the present invention, a novel glass slide suitable for microscopic observation of plant root hair morphology is provided: the first limiter 2012 and the second limiter 2013 have different lengths, the first limiter 2012 and the second limiter 2013 are fixedly arranged at intervals on one side wall of the root segment receiving groove 201, and the second limiter 2013 and the first limiter 2012 are fixedly arranged at intervals on the other side wall of the root segment receiving groove 201, and the sum of the lengths of the first limiter 2012 and the second limiter 2013 is less than the distance between the two side walls of the root segment receiving groove 201.
[0035] In this utility model, the five grooves arranged equidistantly and parallel along the length of the auxiliary piece 2 are the root segment receiving grooves 201, which are 22 mm long, 6 mm wide, 1 mm deep and 6 mm apart, and are used to receive plant root segments.
[0036] Each root segment receiving groove 201 is provided with three opposing first limiters 2012 and second limiters 2013 to prevent root segment slippage. The lengths of the first limiters 2012 and the second limiters 2013 are 2 mm and 3 mm, respectively, the widths are both 0.5 mm, and the heights are both 1 mm. The middle part of the root segment receiving groove 201 is a microscopic observation area.
[0037] The left side wall of the root segment receiving groove 201 is fixedly provided with a second limiter 2013, a first limiter 2012, and a second limiter 2013 in sequence from top to bottom. The left side wall of the root segment receiving groove 201 is fixedly provided with a first limiter 2012, a second limiter 2013, and a first limiter 2012 in sequence from top to bottom.
[0038] The drainage channel 202 is located in a groove 1 mm wide and 1 mm deep on one side edge of the auxiliary plate 2, which is used to guide excess liquid out.
[0039] The outlet 203 is located at the right end of the auxiliary slide 2 and is connected to the drainage channel 202, which facilitates the discharge of liquid to the outside of the glass slide.
[0040] According to this utility model, a novel glass slide suitable for microscopic observation of plant root hair morphology is provided: the auxiliary slide 2 is made of transparent and waterproof material.
[0041] The auxiliary piece 2 can be made of glass or other flat, waterproof materials.
[0042] The working principle of this invention is as follows: An auxiliary slide 2 with a pre-cut root segment receiving groove 201 is fixed on a regular glass slide 1. Then, tweezers are used to place the root segments one by one into the root segment receiving groove 201. A limiter ensures that the root segments are arranged in parallel and with consistent spacing. Excess distilled water is added to the root segment receiving groove 201, with the liquid level slightly higher than the groove opening. Surface tension forms a convex liquid layer. A coverslip is then slowly placed over the surface of the auxiliary slide 2 from top to bottom. During this process, excess liquid flows through the drainage channel 202 to the outlet 203 and is eventually absorbed by absorbent paper, preventing air bubbles from remaining. The slide is placed on the stage of an optical microscope. A suitable field of view is selected in the microscopic observation area, and the focus is adjusted under a 4x or 10x objective lens until the root hairs are clearly imaged.
[0043] Finally, it should be noted that the above embodiments are only used to illustrate the technical solutions of this utility model, and not to limit it. Although this utility model has been described in detail with reference to the foregoing embodiments, those skilled in the art should understand that modifications can still be made to the technical solutions described in the foregoing embodiments, or equivalent substitutions can be made to some of the technical features. Such modifications or substitutions do not cause the essence of the corresponding technical solutions to deviate from the spirit and scope of the technical solutions of the embodiments of this utility model.
Claims
1. A novel glass slide suitable for microscopic observation of plant root hair morphology, characterized in that, include: Ordinary glass slide (1); An auxiliary piece (2) is fixedly disposed on one side of the ordinary glass slide (1). The auxiliary piece (2) is provided with a root segment receiving groove (201), a drainage channel (202) and a water outlet (203). Multiple root segment receiving grooves (201) are arranged in an array on the auxiliary piece (2). The root segment receiving grooves (201) are connected to each other through the drainage channel (202). The end of the drainage channel (202) near the edge of the auxiliary piece (2) is connected to the water outlet (203).
2. The novel glass slide for microscopic observation of plant root hair morphology according to claim 1, characterized in that: The width of the auxiliary slide (2) is the same as the size of the ordinary glass slide (1), and the length of the auxiliary slide (2) is less than the length of the ordinary glass slide (1).
3. A novel glass slide for microscopic observation of plant root hair morphology according to claim 1, characterized in that: The root segment receiving groove (201) includes a rectangular groove (2011), a first limiter (2012) and a second limiter (2013). The first limiter (2012) and the second limiter (2013) are fixedly provided on the two opposite side walls of the rectangular groove (2011).
4. A novel glass slide for microscopic observation of plant root hair morphology according to claim 3, characterized in that: The first limiter (2012) and the second limiter (2013) have different lengths. The first limiter (2012) and the second limiter (2013) are fixedly arranged at intervals on one side wall of the root segment receiving groove (201). The second limiter (2013) and the first limiter (2012) are fixedly arranged at intervals on the other side wall of the root segment receiving groove (201). The sum of the lengths of the first limiter (2012) and the second limiter (2013) is less than the distance between the two side walls of the root segment receiving groove (201).
5. A novel glass slide for microscopic observation of plant root hair morphology according to claim 1, characterized in that: The auxiliary sheet (2) is made of a transparent and waterproof material.