Centrifugal device for DF1 cell infection experiment

Abstract

The utility model discloses a centrifugal device for DF1 cell infection experiment, which comprises a support frame and a support disc connected to the top of the support frame, the center of the support disc is rotatably connected with a centrifugal shaft, a support plate is connected with a spring I for supporting the support plate, the top of the centrifugal shaft is connected with a pressing mechanism for pressing a plurality of test tubes, and the spring I is connected with a spring II for supporting the support plate. The pressing mechanism comprises a connecting rod, a rotating cylinder, a movable disc, a sliding block, a second spring, a plurality of L-shaped plates and a plurality of glands, the two ends of each L-shaped plate are connected with the rotating cylinder and the corresponding glands, the sliding block is used for guiding the movable disc, and the second spring is used for driving the movable disc to move in the direction close to the centrifugal shaft. The top of the centrifugal shaft is connected with a locking mechanism used for locking the L-shaped plate. The stability of a plurality of glands can be improved, a plurality of test tubes can be synchronously pressed and fixed, the installation time of the test tubes is shortened, and the working efficiency is improved.

Description

Technical Field

[0001] This invention relates to the field of cell infection experimental technology, specifically to a centrifugation device for DF1 cell infection experiments. Background Technology

[0002] DF1 cells are a passageable chicken fibroblast cell line derived from ELL chicken embryos. These cells are free of avian leukosis virus and sarcoma virus endogenous genes, and are morphologically fibrous. The DF1 cell line is a stable, tumor-free, spontaneously proliferating cell line, widely used in animal virus research, vaccine development, cancer research, and many other fields. Centrifugation is required in DF1 cell infection experiments to separate components in liquid-solid particle or liquid-liquid mixtures.

[0003] The application, numbered 202320164213.2 and titled "An Antigen Raw Material Sample Centrifugation Device," mentions that "the limiting rod 14 can limit and fix the test tube 11 inside the insert 10, and the pin 20 extending out of the pin hole 18 can limit and fix the limiting rod 14." When fixing multiple test tubes 11, the corresponding limiting rods 14 need to be operated sequentially, thus prolonging the installation time of the test tubes. At the same time, when disassembling and assembling the limiting rod 14, the pin 20 needs to be pressed each time, and both hands need to be used for operation, which is time-consuming, laborious, and has poor applicability. Utility Model Content

[0004] The purpose of this invention is to provide a centrifugation device for DF1 cell infection experiments, which has the advantages of simultaneously clamping and fixing multiple test tubes, shortening the test tube installation time, and improving work efficiency, thus solving the problems mentioned in the background art.

[0005] To achieve the above objectives, this utility model provides the following technical solution: a centrifuge device for DF1 cell infection experiments, comprising a support frame and a support plate connected to the top of the support frame. A centrifuge shaft is rotatably connected to the center of the support plate. Multiple centrifuge tubes are connected to the outer side of the centrifuge shaft. A tray is connected inside each centrifuge tube. A spring is connected to the tray for support. A clamping mechanism for pressing multiple test tubes is connected to the top of the centrifuge shaft. The clamping mechanism includes a connecting rod, a rotating cylinder, a moving disk, a slider, a second spring, multiple L-shaped plates, and multiple pressure caps. The two ends of the L-shaped plates are connected to the rotating cylinder and the corresponding pressure caps. The connecting rod is rotatably connected to the centrifuge shaft, and the top of the connecting rod is connected to the moving disk. The slider is used to guide the moving disk. The second spring is used to drive the moving disk to move closer to the centrifuge shaft. A locking mechanism for locking the L-shaped plates is connected to the top of the centrifuge shaft.

[0006] Preferably, a motor is connected inside the support frame, and the power output end of the motor is connected to the centrifugal shaft.

[0007] Preferably, the clamping mechanism further includes a guide groove, an inner cavity, and an end cap. The guide groove and the inner cavity are both disposed inside the rotating cylinder, and the guide groove is connected to the inner cavity. The movable disk is slidably connected to the inner cavity, the slider is slidably connected to the guide groove, the slider is fixedly connected to the movable disk, and the end cap is used to drive the rotating cylinder to rotate.

[0008] Preferably, the second spring is located inside the inner cavity, and both ends of the second spring are connected to the movable disk and the end of the inner cavity.

[0009] Preferably, the end cap is connected to the top of the rotating cylinder.

[0010] Preferably, the locking mechanism includes a baffle, an L-shaped seat, a connecting groove, a stud, and a handle. The L-shaped seat and the baffle are connected to the top of the centrifugal shaft. The connecting groove is located on the top of the L-shaped seat and matches the L-shaped plate. The handle is used to drive the stud to rotate. The end of the stud matches a positioning groove located on one side of the L-shaped plate.

[0011] Preferably, the handle is fixed to the end of the stud, and the stud is threadedly connected to the baffle.

[0012] Preferably, the baffle is fixedly connected to the L-shaped seat, and the height of the baffle is greater than the height of the L-shaped seat.

[0013] Preferably, a limiting groove is provided inside the centrifuge tube, and the tray is slidably connected to the limiting groove.

[0014] Preferably, both ends of the spring are connected to the tray and the centrifuge cylinder.

[0015] Compared with the prior art, the beneficial effects of this utility model are as follows: This utility model is equipped with a pressing mechanism and a locking mechanism. The pressing mechanism includes a connecting rod, a rotating cylinder, a moving disk, a slider, a second spring, multiple L-shaped plates, multiple pressure caps, a guide groove, an inner cavity, and an end cap. The locking mechanism includes a baffle, an L-shaped seat, a connecting groove, a stud, and a handle. Multiple test tubes are inserted into their respective centrifuge tubes. Then, the rotating cylinder is rotated, causing the multiple L-shaped plates to rotate synchronously, thus rotating the multiple pressure caps. The multiple pressure caps are positioned directly above their respective centrifuge tubes. At this time, the L-shaped plates are aligned with the connecting grooves and fit against the baffle. Then, downward pressure is applied to the rotating cylinder, and the second spring is gradually compressed. The pressure caps are pressed against the test tubes and connected to the top of the centrifuge tubes. The support plate moves down, and the first spring is in a compressed state. The L-shaped plates are connected to the connecting grooves. Rotating the handle drives the stud to rotate, so that the end of the stud connects with the positioning groove, thereby locking the L-shaped plates. This improves the stability of the pressure caps, achieves synchronous pressing and fixing of multiple test tubes, shortens the test tube installation time, and improves work efficiency. Attached Figure Description

[0016] Figure 1 This is a perspective view of the present utility model;

[0017] Figure 2 This is a schematic diagram of the internal structure of the centrifuge tube of this utility model;

[0018] Figure 3 This is a schematic diagram of the L-shaped plate structure of this utility model;

[0019] Figure 4 This is a schematic diagram of the locking mechanism structure of this utility model;

[0020] Figure 5 This is a schematic diagram of the connection structure between the pressing mechanism and the centrifugal shaft of this utility model;

[0021] Figure 6 for Figure 5 Enlarged view of point A in the middle.

[0022] In the diagram: 1. Support frame; 2. Support plate; 3. Motor; 4. Centrifuge shaft; 5. Centrifuge cylinder; 6. Pressure cap; 7. Connecting rod; 8. Rotating cylinder; 9. End cap; 10. L-shaped plate; 11. Baffle; 12. Limiting groove; 13. Support plate; 14. Spring 1; 15. Positioning groove; 16. L-shaped seat; 17. Connecting groove; 18. Stud; 19. Handle; 20. Spring 2; 21. Guide groove; 22. Inner cavity; 23. Moving plate; 24. Slider. Detailed Implementation

[0023] The technical solutions of the present utility model will be clearly and completely described below with reference to the accompanying drawings of the embodiments. Obviously, the described embodiments are only some embodiments of the present utility model, and not all embodiments. Based on the embodiments of the present utility model, all other embodiments obtained by those of ordinary skill in the art without creative effort are within the protection scope of the present utility model.

[0024] Please see Figures 1 to 6This utility model provides a centrifugation device for DF1 cell infection experiments, including a support frame 1 and a support plate 2 connected to the top of the support frame 1. A centrifuge shaft 4 is rotatably connected to the center of the support plate 2. Multiple centrifuge tubes 5 are connected to the outside of the centrifuge shaft 4. Four centrifuge tubes 5 can be provided, with an adjacent included angle of 90 degrees. A support plate 13 is connected inside the centrifuge tube 5. A spring 14 for supporting the support plate 13 is connected to the support plate 13. A clamping mechanism for clamping multiple test tubes is connected to the top of the centrifuge shaft 4. The clamping mechanism includes a connecting rod 7, a rotating cylinder 8, a moving disk 23, a slider 24, a second spring 20, multiple L-shaped plates 10, and multiple pressure caps 6. The two ends of the L-shaped plates 10 are connected to the rotating cylinder 8 and the corresponding pressure caps 6. The connecting rod 7 is rotatably connected to the centrifuge shaft 4, and the top of the connecting rod 7 is connected to the moving disk 23. The slider 24 is used to guide the moving disk 23. The second spring 20 is used to drive the moving disk 23 to move closer to the centrifuge shaft 4. A locking mechanism for locking the L-shaped plates 10 is connected to the top of the centrifuge shaft 4.

[0025] Multiple test tubes are inserted into their respective centrifuge tubes 5. Then, rotating the rotating cylinder 8 drives multiple L-shaped plates 10 to rotate synchronously, thereby rotating multiple pressure caps 6. The multiple pressure caps 6 are positioned directly above their respective centrifuge tubes 5. At this time, the L-shaped plates 10 are aligned with the connecting grooves 17 and adhere to the baffles 11. Then, downward pressure is applied to the rotating cylinder 8, and the second spring 20 is gradually compressed. The pressure caps 6 are pressed tightly against the test tubes and connected to the top of the centrifuge tubes 5. The support plate 13 moves down and the first spring 14 is in a compressed state. The L-shaped plates 10 are connected to the connecting grooves 17. Rotating the handle 19 drives the stud 18 to rotate, so that the end of the stud 18 is connected to the positioning groove 15, thereby locking the L-shaped plates 10 and improving the stability of the pressure caps 6. This achieves synchronous pressing and fixing of multiple test tubes, shortens the test tube installation time, and improves work efficiency.

[0026] When the test tube needs to be disassembled, the rotary handle 19 drives the stud 18 to rotate, causing the end of the stud 18 to separate from the positioning groove 15, thereby loosening the L-shaped plate 10. The compressed spring 20 quickly returns to its original position, which can lift the rotating cylinder 8, thereby driving multiple L-shaped plates 10 and the pressure cap 6 to rise and return to their original positions, so that the pressure cap 6 separates from the centrifuge cylinder 5. The compressed spring 14 drives the support plate 13 to move upward, and the support plate 13 drives the test tube to move upward, so that the top of the test tube protrudes from the top of the centrifuge cylinder 5. Then, the rotating cylinder 8 is rotated to drive multiple pressure caps 6 to rotate synchronously in the X direction, so that the pressure cap 6 is misaligned with the corresponding centrifuge cylinder 5, thereby making it easier to remove the test tube from the centrifuge cylinder 5.

[0027] A motor 3 is connected inside the support frame 1. The power output end of the motor 3 is connected to the centrifugal shaft 4. The motor 3 can drive the centrifugal shaft 4 to rotate, thereby driving the four centrifugal tubes 5 to rotate synchronously, so as to realize the centrifugation operation of the solvent in the test tube.

[0028] The clamping mechanism also includes a guide groove 21, an inner cavity 22, and an end cap 9. Both the guide groove 21 and the inner cavity 22 are located within the rotating cylinder 8, with the guide groove 21 communicating with the inner cavity 22. A movable disk 23 is slidably connected to the inner cavity 22, and a slider 24 is slidably connected to the guide groove 21. The slider 24 is fixedly connected to the movable disk 23. The end cap 9 is connected to the top of the rotating cylinder 8. The outer edge of the end cap 9 is concave-convex to facilitate rotation of the end cap 9, and the rotation of the end cap 9 synchronously drives the rotation of the rotating cylinder 8 in the same direction. The slider 24 provides good guidance for the movable disk 23, meaning that the rotating cylinder 8 can only move up and down relative to the connecting rod 7, but cannot rotate.

[0029] Spring 20 is located inside the inner cavity 22, and both ends of spring 20 are connected to the movable disk 23 and the end of the inner cavity 22.

[0030] The locking mechanism includes a baffle 11, an L-shaped seat 16, a connecting groove 17, a stud 18, and a handle 19. The L-shaped seat 16 and the baffle 11 are connected to the top of the centrifuge shaft 4. The connecting groove 17 is located on the top of the L-shaped seat 16 and matches the L-shaped plate 10. The handle 19 is used to drive the stud 18 to rotate, and the end of the stud 18 matches the positioning groove 15 located on one side of the L-shaped plate 10. During the process of fixing the test tube, rotating the rotating cylinder 8 can drive the four L-shaped plates 10 to rotate in the opposite direction of X. When the L-shaped plate 10 is in contact with the baffle 11, the four pressure caps 6 are located directly above the corresponding centrifuge tubes 5. That is, the baffle 11 limits the L-shaped plate 10, making it convenient for people to operate the rotating cylinder 8. At this time, the L-shaped plate 10 and the connecting groove 17 are aligned.

[0031] The handle 19 is fixed to the end of the stud 18, which is threadedly connected to the baffle 11. When the L-shaped plate 10 is connected to the connecting groove 17, the end of the stud 18 is aligned with the positioning groove 15.

[0032] The baffle 11 is fixedly connected to the L-shaped seat 16, and the height of the baffle 11 is greater than the height of the L-shaped seat 16.

[0033] A limiting groove 12 is provided inside the centrifuge cylinder 5, and the tray 13 is slidably connected to the limiting groove 12 to improve the stability of the tray 13 in raising and lowering.

[0034] The two ends of spring 14 are connected to tray 13 and centrifuge cylinder 5.

[0035] Working principle: Multiple test tubes are inserted into their respective centrifuge tubes 5, with their bottoms supported by the support plate 13. The rotating end cap 9 drives the rotating cylinder 8 and connecting rod 7 to rotate in the opposite direction of X, which in turn drives the rotation of multiple pressure caps 6. When the L-shaped plate 10 is attached to the baffle 11, the pressure cap 6 is directly above the corresponding centrifuge tube 5, applying downward pressure to the rotating cylinder 8. The moving disk 23 moves upward relative to the rotating cylinder 8, the slider 24 slides within the guide groove 21, and the second spring 20 is gradually compressed. When the L-shaped plate 10 is connected to the L-shaped seat 16, i.e., the L-shaped plate 10 is connected to the connecting groove 17, the pressure cap 6 presses tightly against the test tube, the support plate 13 moves downward, and the first spring 14 is compressed. The rotating handle 19 drives the stud 18 to rotate, connecting the end of the stud 18 to the positioning groove 15, thus locking the L-shaped plate 10 and improving the stability of the pressure cap 6, achieving synchronous pressing and fixing of multiple test tubes. Then, motor 3 drives centrifuge shaft 4 to rotate, causing multiple centrifuge tubes 5 to rotate synchronously, realizing the centrifugation of solvent in the test tubes. When centrifugation is completed, i.e., when the test tubes need to be disassembled, first turn handle 19 to drive stud 18 to rotate, so that the end of stud 18 separates from positioning groove 15. At this time, L-shaped plate 10 is in a loosened state, and compressed spring 20 quickly returns to its original position, which can lift rotating cylinder 8, so that multiple caps 6 rise and separate from centrifuge tubes 5. Then turn rotating cylinder 8 to drive multiple caps 6 to return to their original position synchronously. The rotation direction is X, so that caps 6 are misaligned with centrifuge tubes 5. Compressed spring 14 lifts support plate 13, realizing the rise of test tubes, i.e., the top of the test tube protrudes from the top of centrifuge tube 5, making it easy to remove from centrifuge tube 5. The operation is simple and flexible.

[0036] Although embodiments of the present invention have been shown and described, it will be understood by those skilled in the art that various changes, modifications, substitutions and alterations can be made to these embodiments without departing from the principles and spirit of the present invention, the scope of which is defined by the appended claims and their equivalents.

Claims

1. A centrifuge device for DF1 cell infection experiments, characterized in that, The system includes a support frame (1) and a support plate (2) connected to the top of the support frame (1). A centrifugal shaft (4) is rotatably connected to the center of the support plate (2). Multiple centrifugal tubes (5) are connected to the outside of the centrifugal shaft (4). A tray (13) is connected inside the centrifugal tube (5). A spring (14) for supporting the tray (13) is connected to the tray (13). A clamping mechanism for clamping multiple test tubes is connected to the top of the centrifugal shaft (4). The clamping mechanism includes a connecting rod (7), a rotating cylinder (8), a moving disk (23), and a slider (2). 4) Spring 2 (20), multiple L-shaped plates (10), multiple pressure caps (6), the two ends of the L-shaped plates (10) are connected to the rotating cylinder (8) and the corresponding pressure caps (6), the connecting rod (7) is rotatably connected to the centrifugal shaft (4), and the top of the connecting rod (7) is connected to the moving disk (23), the slider (24) is used to guide the moving disk (23), the spring 2 (20) is used to drive the moving disk (23) to move closer to the centrifugal shaft (4), and the top of the centrifugal shaft (4) is connected to a locking mechanism for locking the L-shaped plates (10).

2. The centrifuge device for DF1 cell infection experiments according to claim 1, characterized in that, The support frame (1) is connected to a motor (3), and the power output end of the motor (3) is connected to the centrifugal shaft (4).

3. The centrifuge device for DF1 cell infection experiments according to claim 1, characterized in that, The clamping mechanism also includes a guide groove (21), an inner cavity (22), and an end cap (9). The guide groove (21) and the inner cavity (22) are both located inside the rotating cylinder (8), and the guide groove (21) is connected to the inner cavity (22). The movable disk (23) is slidably connected to the inner cavity (22), and the slider (24) is slidably connected to the guide groove (21). The slider (24) is fixedly connected to the movable disk (23), and the end cap (9) is used to drive the rotating cylinder (8) to rotate.

4. A centrifuge apparatus for DF1 cell infection experiments according to claim 3, characterized in that, The second spring (20) is located inside the inner cavity (22), and both ends of the second spring (20) are connected to the moving disk (23) and the end of the inner cavity (22).

5. A centrifuge apparatus for DF1 cell infection experiments according to claim 3, characterized in that, The end cap (9) is connected to the top of the rotating cylinder (8).

6. A centrifuge apparatus for DF1 cell infection experiments according to claim 1, characterized in that, The locking mechanism includes a baffle (11), an L-shaped seat (16), a connecting groove (17), a stud (18), and a handle (19). The L-shaped seat (16) and the baffle (11) are connected to the top of the centrifugal shaft (4). The connecting groove (17) is located on the top of the L-shaped seat (16) and matches the L-shaped plate (10). The handle (19) is used to drive the stud (18) to rotate. The end of the stud (18) matches the positioning groove (15) located on one side of the L-shaped plate (10).

7. A centrifuge apparatus for DF1 cell infection experiments according to claim 6, characterized in that, The handle (19) is fixed to the end of the stud (18), which is threadedly connected to the baffle (11).

8. A centrifuge apparatus for DF1 cell infection experiments according to claim 6, characterized in that, The baffle (11) is fixedly connected to the L-shaped seat (16), and the height of the baffle (11) is greater than the height of the L-shaped seat (16).

9. A centrifuge apparatus for DF1 cell infection experiments according to claim 1, characterized in that, The centrifuge tube (5) is provided with a limiting groove (12), and the tray (13) is slidably connected to the limiting groove (12).

10. A centrifuge apparatus for DF1 cell infection experiments according to claim 1, characterized in that, The two ends of the spring (14) are connected to the tray (13) and the centrifuge cylinder (5).

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